Caroline Hennings. NATURSTOFF-TECHNIK GMBH, Alemania.

1. BioTRANSformation of by-products from fruit and
vegetable processing industry into valuable
BIOproducts
A European Project supported within the Seventh Framework Programme for Research and Technological Development
SSF FROM LAB TO FINAL APPLICATION
Caroline Hennigs, Naturstoff-Technik GmbH
03.11.2015, Final Conference TRANSBIO, Vitoria-Gasteiz (Spain)

2. Introduction
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Where is NST
NST locations
• Dellstedt
• Bremerhaven
• Osterholz-Scharmbeck
Germany

3. Introduction
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Who is NST
● founded 01.10.1979 in Ritterhude
● take over 01.07.2008 from Mr. Holmer Wöhlk
● Opening of an R&D department in Bremerhaven in April 2009
● contract manufacturing in
– Drying (Fluid Bed Dryer)
– Agglomeration
– Spraygranulation
– Encapsulation / Coating
– Mixing
– Milling
– Sieving
– Process development (Bremerhaven)
● 2 employees at 01.07.2008 / currently 7 employees + temporary staff
● production area 1100 qm
● Plants: - WSG 5 / 60 / 120 / 500
- continuous belt dryer/cooler (1-2 t/h)
- Solid State Fermentation plant (solid state fermentation in Dellstedt)

4. • Possibility for tests in technical
scale in a good, fast and cheap way.
NST R&D department www.bio-nord.de
4
Introduction

5. General Information
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Solid-state fermentation is used for the production of
microbial products such as
• Feed
• Fuel
• Food
• Industrial chemicals
• Pharmaceutical products

6. Solid state fermentation at lab scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development
SSF at lab scale: Koji production process
.
Washing
Soaking
Rice steaming
Inoculation
Koji cultivation
Raw product
soaking
steaming
Inoculation
cultivation

7. Solid state fermentation at lab scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development

8. Koji- Rice
Koji Soft drink
Vinegar
Baby
mush
Application SSF products
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Probiotic grain drink
Koji Rice wine (Sake)
Animal food (Agricolia)
Sweet white miso
Sojasoße
Black garlic
Medicine
(immunsystem)
Eggs
Amazake

9. Koji production
A European Project supported within the Seventh Framework Programme for Research and Technological Development
SSF = Solid State Fermenter
Tray fermentation systemKoji made by hand since 2000years

10. Lab- scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development
A better aeration is possible
by bioreactor upscale
Try- bioreactor 0.5 – 1.0 kg
Fermenter prototype

11. Tray fermentation process
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Reactor funktion (Lab scale)
•Chamber is incubated in a temperature-controlled room
•Water saturated air is added from the top.
•The trays fit inside the reactor and conditioned air is forced in at
the lower left portion of the reactor.
•The reactor is equipped with thermocouples, flow meter, oxygen
sensor and relative humidity sensor.
.

12. A European Project supported within the Seventh Framework Programme for Research and Technological Development
Listing of Substrates,
Microorganisms and
Enzymes produced

13. Advantages SSF
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Advantages compared to submerged fermentation (General)
• Low production cost due to
• saving of water and energy
• less waste effluent problem
• Improved product stability due to lower dilution in the medium
• Various applications of fermentation products
Food
Feed
Pharmaceuticals
Industrial detergents
 Holistic utilisation of the product = increased sustainability

14. Up- scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Washing and soaking of
rice grains
Rice grain
steaming
Cool down and
Inocculation with
spores
Scale up to SSF at technical scale (600kg)

15. Up- scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Scale up to SSF at technical scale (600kg)
SensorSpinning plateStirrer
Raw product inlet
Air cooling unit

16. Comparison Lab/ Technical scale
=> Koji rice ferment’s enzyme activity is extremely high
=> Activity was even significantly increased by scale‐up
Lab scale (50 kg) Technical scale (600 kg)
Yield 108 – 110% 113 – 115%
Protease activity [U/mg] 106 1785
Lipase activity [U/mg] 30 800
Amilase [U/mg] 0,02 0,17
Up- scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development

17. Up- scale
A European Project supported within the Seventh Framework Programme for Research and Technological Development
Scale up to SSF at industrial scale (1.200kg)
 By numbering up

18. A European Project supported within the Seventh Framework Programme for Research and Technological Development
Thank you very much for your attention!
Caroline Hennigs
Naturstoff-Technik GmbH
Marie-Curie Str. 11
27711 Osterholz-Scharmbeck
Germany