2. A Protocol for Computer-Based Protein Structure and Function
Prediction
A unified platform for automated protein structure
I-TASSER Server is an internet service for protein
structure and function predictions. It allows academic
users to automatically generate high-quality
predictions of 3D structure and biological function
of protein molecules from their amino acid
sequences.
3. I-Tasser helps in:
• Protein Structure Prediction
• Struture-based Function Annotation
• Protein Design
• Modeling of G Protein-Coupled Receptor and Ligand-Receptor Interactions
• Amyloid Diseases and Fiber Aggregation
• Modeling of Protein-Protein Interactions
• RNA Alternative Splicing
• Ligand Screening and Structure-Based Drug Design
4. • It follows the sequence-to-structure-to-function paradigm.
• Procedure for structure and function modeling involves :
Template identification by LOMETS
Fragment Structure reassembly by
replica-exchange Monte-Carlo simulations
Atomic level model
construction of structure refinement
Structure based function
interpretations using COFACTOR
5. How to submit a query sequence?
Step 1: Open I-TASSER Server
6. Step 2: Enter Sequence and mandatory fields then Run I-TASSER
