Pilot Study Results

1. Transdermal Delivery of Carbon
Dioxide Boosts Microcirculation
Pilot Study Results
"There are a lot of possibilities here and we are really excited about
conducting future studies to determine what D`OXYVA may be able to
provide to people with microvascular disease."
Presentation by Judy M. Delp, Ph.D.
Associate Professor, Department of Physiology and Functional Genomics,
University of Florida, USA
InvisiDerm D`OXYVA Scientific Advisory Board Member
July 2013

2. Oxygen Delivery Depends on:
1. Heart
2. Arteries
3. Arterioles
4. Capillaries
Arteriole
Smooth muscle cells
Decreased
resistance
Smooth
muscle
relaxation
Normal
resting
tone
Smooth
muscle
contraction
Increased
resistance
Smooth
muscle
cell
Interior
of arteriole

3. Study Design:
Subjects:
6 subjects with diabetes
8 subjects without diabetes
Treatment:
The subject’s thumb was inserted into
the D`Oxyva® device and “bathed” in
CO2 gas for 5 minutes.
Measurements:
Brachial blood pressure
A graph displays pressure and perfusion
during cuff deflation and indicates the
pressure at which skin perfusion is found to
return.
SensiLase® System
Skin perfusion pressure in the toe
•5 minutes pre-treatment
•5 min post-treatment
•30 minutes post-treatment
•60 minutes post-treatment
•120 minutes post-treatment
•240 minutes post-treatment

4. BASELINE MEASUREMENTS
Diabetic Non-Diabetic
SystolicBP(mmHg)
0
20
40
60
80
100
120
140
Diabetic Non-Diabetic
DiastolicBP(mmHg)
0
20
40
60
80
100
Blood Pressure
Diabetic Non-Diabetic
SPP(mmHg)
0
20
40
60
80
100
Skin Blood Flow

5. Transdermal delivery of CO2 to the thumb
promotes sustained blood flow at the foot.
Time (minutes)
-5 5 30 60 120 240
ChangeinSPP(mmHg)
0
10
20
30
40
50 Diabetic (n =6)
Non-Diabetic (n=8)
*
*
* *
*
B

6. How does transdermal CO2 delivered at the
thumb cause blood flow to the foot to increase?
1. Neural Signals
 Metaboreflex
2. Hormonal Signals
 Epinephrine
 Angiotensin
3. Tissue Signals
 Adenosine
 Potassium }

7. A FEW BENEFITS OF IMPROVED
MICROVASCULAR FUNCTION
• Better Healing
• Pain Reduction
• Increased Metabolism
• Reduction of Fatigue