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MUHAMMED ASLAM
2014-12-127
Seed treatment refers to the application of fungicide , insecticide
or both to the seeds to disinfect (deep seated ) and disinfest
( over seed coat ) them from seed borne or soil borne pathogenic
organisms and storage insects.
Benefits of seed treatment
1.Prevention of spread of plant diseases both systemic
and non systemic
2.Protects seed from seed rot and seedling blights
3. Improves germination
4. Provides protection from storage insects and pests.
Dates back to 17th century when salt water was accidently
discovered to control bunt and stinking smut of wheat seed.
In 1755, M.D Tillet, a french botonist recommented the use of
lye and lime as a chemical treatment for wheat seed
Some 50 years later ,Prevost a Swiss botanist ,discovered the
use of copper fungicides as seed treatment.
New concepts of treating seeds with Organomercurials like
Ceresan and Semesan were introduced in 1920.
1.SYSTEMIC
DISEASES
2.NON
SYSTEMIC
DISEASES
3.SEED ROT
AND
SEEDLING
BLIGHTS
4. STORAGE
AND SOIL
INSECTS
The major diseases and insect pests that can be
controlled by seed treatment are
Stinking smut of wheat
Loose smut of wheat Helminthosporium stripe of barley
Loose and covered smut of oats
Helminthosporium spot of RICE and Wheat
Wire worms
Seed corn maggots
Hanson et al. [1] divided seed treatment methods into three
groups
MECHANICAL METHODS
PHYSICAL METHODS
CHEMICAL METHODS
OTHER METHODS
1. MECHANICAL METHODS
These are designed to remove infectious materials mixed
with seeds.
Seeds can be mechanically cleaned thoroughly before
seeding to remove pathogenic organisms from the seed
surfaces.
This often requires further treatment.
2. PHYSICAL METHODS
 Physical methods include hot-water and water-soak
treatments and ultraviolet, infrared, X-ray and other types of
irradiation.
 Do not protect seeds from soilborne organisms
3. CHEMICAL METHODS
Most commonly used method because of its
effectiveness and ease of handling.
IDEAL CHEMICAL would be;
Highly effective in controlling the pathogen
Harmless to the seed,animals and non toxic to people
Economic to use
Easy to apply
Non corrosive to machinery
Stable for long periods
A) Mercurial Compounds
1. Organo mercurials: Small grains , flax cotton and
safflower
Eg: PMA (Phenyl Mercuric acetate), MEMC (Methoxy
ethyl Mercury chloride), EMC (Ethyl mercuric chloride )
2.Inorganic mercurials: Mercuric chloride, mercurious
chloride and mercuric oxide
Tuber and root crops , Garden and vegetable crops
Chemicals used for Seed treatment
B) Non Mercurials
1.Organic non mercurials
 Less effective than the organic mercurials
 Less damaging to the seeds
 Less damaging to the persons handling the seeds
Over dose is not harmful and viability is not effected
Eg Thiram, Captan, Carbendazim, Metalaxyl
2. Inorganic Non Mercurials:
Copper carbonate, Copper sulphate – Bunt disease in
wheat
Cuprous oxide- seed decay and damping off in
vegetables
I. Chemical treatments to improve germination and
vigour potential
Soaking / treating the seeds with nutrients vitamins and
micronutrients etc
1.Paddy: Seeds can be soaked in 1 % KCl solution for 12 hours
to improve the germination and vigour potential.
2.Sorghum: Seeds could be soaked in NaCl2 (1 %) or KH2PO4
(1%) for 12 hours for improving the germination and vigour
potential.
3.Pulses : Seeds can be soaked in ZnSO4, MgSO4
and MnSO4 100 ppm solution for 4 hours to improve
the germination and vigour potential.
4. OTHER METHODS
ABCD specifically controls Fusarium, Alternaria, Drechslera,
Aspergillus flavus
It was also very effective in controlling the major stored grain
pests
ABCD was also found effective in reducing seedborne
diseases
Siddiqui and Agarwal [2] brought to light a
new material for seed treatment, ABCD
(attapulgite - based clay dust), developed
at the Indian Institute of Chemical
Technology (CSIR, Hyderabad) for seed
treatment and safe storage of seeds and
food grains.
1. Seed disinfection: It refers to eradication of
fungal spores present within the seed coat or
more deep seated tissues
2. Seed disinfestations: It refers to the
destruction of surface borne organisms that
contaminated the seed surface but not
infected the seed.
3. Seed protection: To protect the seed and
young seedling from organisms in the soil
which might otherwise cause delay of the
seed before germination.
Seed Treatment Fungicides
Equipments for seed treatment
1 .Slurry treaters
 A slurry is prepared by mixing the
chemical with water.
 The treatment material to be
applied as slurry is accurately
measured through a simple
mechanism comprised of a slurry cup
and seed dump pan.
 The cup introduces a given amount
of slurry with each dump of seed into
a mixing chamber where the seeds are
mixed thoroughly.
2.Direct treater
 Panogen and Mist o matic treater
The "mist-o-matic" treater applies chemical as a mist
directly to the seed.
The treater is equipped with a large treatment tank, a
pump and a return that maintains the level in the small
reservoir from which the seed is fed.
 A simple mixer can be made
by running a pipe through a
drum at an angle.
 The drum is then mounted
onto a stand.
 The seed and treatment are
placed in the drum and it is
rotated slowly until all the seeds
are cornered with the chemicals.
3. Drum Mixer
Liquid materials can be dripped on the seed as they
enter a grain auger or straw conveyer.
By the time seeds have left the auger the chemical is
spread on the seeds.
4. Grain Auger
5. Shovel
Seeds are spread on a clean dry surface 10-15 cm in depth.
The proper amount of chemical is diluted with water and
sprinkled over the seed.
Mixing is done with shovel or sloop turning the seed at
least 20 times.
1. SEED
COATING
Seed coating is the application of useful
materials to the seed without changing its
general size or shape.
Seed coating has been utilised to ameliorate environmental
stresses such as drought and flooding
Seed coating with peroxide compounds provide oxygen to
seeds under anoxic soil conditions
Hydrophilic polymers maintain a high water potential
around germinating seeds
2. SEED PELLETING
Pelleting is enclosing of seed in a filler material using an
adhesive with bioactive chemicals to ensure singling of seed and
size increases for easy handling.
The binders for pelleting include gum arabic ,gelatin, starch
and the commonly used fillers are Lime stone, gypsum, talc,
vermiculite, peat etc.
The pellet may act as a physical barrier as well as mechanical
barrier.
3. ENCAPSULATION OF SEEDS
Natural seeds also can be
encapsulated using hydrogels
Redenbaugh et al.[3] mixed
propagules with sodium alginate
solutions and then transferred
the coated propagules to a
calcium salt to form a soft
capsule.
This technology was developed to form
capsules of somatic embryos by gelation to
enhance their stability
4. FLUID DRILLING AND SEED PRIMING
It consist of germinating
seeds in aerated water until
radicle emergence
The seeds are then mixed in
a viscous gel and sown with
an appropriate drill.
The gel prevents seeding
injury to the emerging
radicle and maintains seed
moisture
Seed priming allows for the regulation of the water content in
the seed, either by soaking the seeds in water or in a solute; or, by
exposing the seeds to water vapor.
Osmoticums such as PEG and inorganic salts have
been used as a priming agents
o Hydropriming
o Osmotic priming.
o Solid matrix priming.
o Drum priming
The seeds imbibe water for a time
interval. After the time interval, the process
is halted right before the first root, called
the radicle, emerges from the seed.
With priming the germination process is
activated so that seeds germinate more
rapidly and emerge more uniformly
5. BIOLOGICAL SEED
TREATMENTS
Seed treatment with beneficial microorganisms like
Rhizobium spp. to treat leguminous seeds for nitrogen
fixation and Azospirillum and other nitrogen –fixing
bacteria has long been known.
Fathey [5] noted that an endophytic bacterium,
Clavibacter xylii, when introduced into corn seeds is
transformed to produce an insecticidal protien (§-endo
toxin) from Bacillus thuringenesis which is active against
the european corn borer
As a warning that the seeds
have been treated .
The colour also indicate the
uniformity of the chemical
coverage.
Coloring of seeds prevents
inadvertant use of treated
seeds for food or feed
purpose.
Coloring of seeds
 Never used for animal or human consumption.
 The treated seeds must be properly labelled.
 Care must be taken to treat the seeds at correct dosage.
 Do not treat the seed with concentrated liquid
products.
 The technique must be economical and practical for the
specific crop, and above all the materials used should be
environmentally safe
Precautions for seed treatment!!!
Name of
Crop
Pest/Disease Seed Treatment
Sugarcane Root rot, wilt Carbendazim (0.1%) 2 gm/kg seed
Trichoderma spp. 4-6 gm/kg. seed
Rice Root rot disease
other
insects/pests
Bacterial shealth
blight
Trichoderma 5-10 gm/kg. seed
(before transplanting)
Chloropyriphos 3gm/kg seed.
Pseudomonas flourescens0.5% W.P.
10 gm/kg.
Chillies Anthracnose spp.
Damping off
Seed treatment with
Trichoderma viride 4g/kg,
Carbandazim @ 1g/100 gm seed.
Tomato Soil borne
infection of
fungal disease
Early blight
Damping offWilt
T. viride @ 2 gm/100gm seed.
Captan 75 WS @ 1.5 to 2.0 gm a.i./litre
for soil drenching.Pseudomonas
fluorescens and V. clamydosporium @
10gm/kg as seed dresser.
Crucifero
us veget
ables
(Cabbage
Cauliflo
wer,
Broccoli,
Knol-
khol,
radish)
Soil /
Seed borne diseas
es (Damping
off)Root knot
nematode
Seed treatment with Trichoderma
viridi @ 2 g / 100 g seeds
Captan 75% WS @ 1.5 to 2.5 gm
a.i./litre for soil
drenching.Pseudomonas fluorescens
and Verlicillium clamydosporium @
10gm/kg seed as seed dresser
Seed treatment by Muhammed Aslam COH,Thrishur

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Seed treatment by Muhammed Aslam COH,Thrishur

  • 2. Seed treatment refers to the application of fungicide , insecticide or both to the seeds to disinfect (deep seated ) and disinfest ( over seed coat ) them from seed borne or soil borne pathogenic organisms and storage insects. Benefits of seed treatment 1.Prevention of spread of plant diseases both systemic and non systemic 2.Protects seed from seed rot and seedling blights 3. Improves germination 4. Provides protection from storage insects and pests.
  • 3. Dates back to 17th century when salt water was accidently discovered to control bunt and stinking smut of wheat seed. In 1755, M.D Tillet, a french botonist recommented the use of lye and lime as a chemical treatment for wheat seed Some 50 years later ,Prevost a Swiss botanist ,discovered the use of copper fungicides as seed treatment. New concepts of treating seeds with Organomercurials like Ceresan and Semesan were introduced in 1920.
  • 4. 1.SYSTEMIC DISEASES 2.NON SYSTEMIC DISEASES 3.SEED ROT AND SEEDLING BLIGHTS 4. STORAGE AND SOIL INSECTS The major diseases and insect pests that can be controlled by seed treatment are
  • 6. Loose smut of wheat Helminthosporium stripe of barley Loose and covered smut of oats
  • 7. Helminthosporium spot of RICE and Wheat
  • 8.
  • 10. Hanson et al. [1] divided seed treatment methods into three groups MECHANICAL METHODS PHYSICAL METHODS CHEMICAL METHODS OTHER METHODS
  • 11. 1. MECHANICAL METHODS These are designed to remove infectious materials mixed with seeds. Seeds can be mechanically cleaned thoroughly before seeding to remove pathogenic organisms from the seed surfaces. This often requires further treatment.
  • 12. 2. PHYSICAL METHODS  Physical methods include hot-water and water-soak treatments and ultraviolet, infrared, X-ray and other types of irradiation.  Do not protect seeds from soilborne organisms
  • 13. 3. CHEMICAL METHODS Most commonly used method because of its effectiveness and ease of handling. IDEAL CHEMICAL would be; Highly effective in controlling the pathogen Harmless to the seed,animals and non toxic to people Economic to use Easy to apply Non corrosive to machinery Stable for long periods
  • 14. A) Mercurial Compounds 1. Organo mercurials: Small grains , flax cotton and safflower Eg: PMA (Phenyl Mercuric acetate), MEMC (Methoxy ethyl Mercury chloride), EMC (Ethyl mercuric chloride ) 2.Inorganic mercurials: Mercuric chloride, mercurious chloride and mercuric oxide Tuber and root crops , Garden and vegetable crops Chemicals used for Seed treatment
  • 15. B) Non Mercurials 1.Organic non mercurials  Less effective than the organic mercurials  Less damaging to the seeds  Less damaging to the persons handling the seeds Over dose is not harmful and viability is not effected Eg Thiram, Captan, Carbendazim, Metalaxyl 2. Inorganic Non Mercurials: Copper carbonate, Copper sulphate – Bunt disease in wheat Cuprous oxide- seed decay and damping off in vegetables
  • 16. I. Chemical treatments to improve germination and vigour potential Soaking / treating the seeds with nutrients vitamins and micronutrients etc 1.Paddy: Seeds can be soaked in 1 % KCl solution for 12 hours to improve the germination and vigour potential.
  • 17. 2.Sorghum: Seeds could be soaked in NaCl2 (1 %) or KH2PO4 (1%) for 12 hours for improving the germination and vigour potential.
  • 18. 3.Pulses : Seeds can be soaked in ZnSO4, MgSO4 and MnSO4 100 ppm solution for 4 hours to improve the germination and vigour potential.
  • 19. 4. OTHER METHODS ABCD specifically controls Fusarium, Alternaria, Drechslera, Aspergillus flavus It was also very effective in controlling the major stored grain pests ABCD was also found effective in reducing seedborne diseases Siddiqui and Agarwal [2] brought to light a new material for seed treatment, ABCD (attapulgite - based clay dust), developed at the Indian Institute of Chemical Technology (CSIR, Hyderabad) for seed treatment and safe storage of seeds and food grains.
  • 20. 1. Seed disinfection: It refers to eradication of fungal spores present within the seed coat or more deep seated tissues 2. Seed disinfestations: It refers to the destruction of surface borne organisms that contaminated the seed surface but not infected the seed. 3. Seed protection: To protect the seed and young seedling from organisms in the soil which might otherwise cause delay of the seed before germination. Seed Treatment Fungicides
  • 21. Equipments for seed treatment
  • 22. 1 .Slurry treaters  A slurry is prepared by mixing the chemical with water.  The treatment material to be applied as slurry is accurately measured through a simple mechanism comprised of a slurry cup and seed dump pan.  The cup introduces a given amount of slurry with each dump of seed into a mixing chamber where the seeds are mixed thoroughly.
  • 23. 2.Direct treater  Panogen and Mist o matic treater The "mist-o-matic" treater applies chemical as a mist directly to the seed. The treater is equipped with a large treatment tank, a pump and a return that maintains the level in the small reservoir from which the seed is fed.
  • 24.
  • 25.  A simple mixer can be made by running a pipe through a drum at an angle.  The drum is then mounted onto a stand.  The seed and treatment are placed in the drum and it is rotated slowly until all the seeds are cornered with the chemicals. 3. Drum Mixer
  • 26. Liquid materials can be dripped on the seed as they enter a grain auger or straw conveyer. By the time seeds have left the auger the chemical is spread on the seeds. 4. Grain Auger 5. Shovel Seeds are spread on a clean dry surface 10-15 cm in depth. The proper amount of chemical is diluted with water and sprinkled over the seed. Mixing is done with shovel or sloop turning the seed at least 20 times.
  • 27.
  • 28. 1. SEED COATING Seed coating is the application of useful materials to the seed without changing its general size or shape. Seed coating has been utilised to ameliorate environmental stresses such as drought and flooding Seed coating with peroxide compounds provide oxygen to seeds under anoxic soil conditions Hydrophilic polymers maintain a high water potential around germinating seeds
  • 29. 2. SEED PELLETING Pelleting is enclosing of seed in a filler material using an adhesive with bioactive chemicals to ensure singling of seed and size increases for easy handling. The binders for pelleting include gum arabic ,gelatin, starch and the commonly used fillers are Lime stone, gypsum, talc, vermiculite, peat etc. The pellet may act as a physical barrier as well as mechanical barrier.
  • 30. 3. ENCAPSULATION OF SEEDS Natural seeds also can be encapsulated using hydrogels Redenbaugh et al.[3] mixed propagules with sodium alginate solutions and then transferred the coated propagules to a calcium salt to form a soft capsule. This technology was developed to form capsules of somatic embryos by gelation to enhance their stability
  • 31. 4. FLUID DRILLING AND SEED PRIMING It consist of germinating seeds in aerated water until radicle emergence The seeds are then mixed in a viscous gel and sown with an appropriate drill. The gel prevents seeding injury to the emerging radicle and maintains seed moisture
  • 32. Seed priming allows for the regulation of the water content in the seed, either by soaking the seeds in water or in a solute; or, by exposing the seeds to water vapor. Osmoticums such as PEG and inorganic salts have been used as a priming agents o Hydropriming o Osmotic priming. o Solid matrix priming. o Drum priming The seeds imbibe water for a time interval. After the time interval, the process is halted right before the first root, called the radicle, emerges from the seed. With priming the germination process is activated so that seeds germinate more rapidly and emerge more uniformly
  • 33. 5. BIOLOGICAL SEED TREATMENTS Seed treatment with beneficial microorganisms like Rhizobium spp. to treat leguminous seeds for nitrogen fixation and Azospirillum and other nitrogen –fixing bacteria has long been known. Fathey [5] noted that an endophytic bacterium, Clavibacter xylii, when introduced into corn seeds is transformed to produce an insecticidal protien (§-endo toxin) from Bacillus thuringenesis which is active against the european corn borer
  • 34.
  • 35. As a warning that the seeds have been treated . The colour also indicate the uniformity of the chemical coverage. Coloring of seeds prevents inadvertant use of treated seeds for food or feed purpose. Coloring of seeds
  • 36.  Never used for animal or human consumption.  The treated seeds must be properly labelled.  Care must be taken to treat the seeds at correct dosage.  Do not treat the seed with concentrated liquid products.  The technique must be economical and practical for the specific crop, and above all the materials used should be environmentally safe Precautions for seed treatment!!!
  • 37. Name of Crop Pest/Disease Seed Treatment Sugarcane Root rot, wilt Carbendazim (0.1%) 2 gm/kg seed Trichoderma spp. 4-6 gm/kg. seed Rice Root rot disease other insects/pests Bacterial shealth blight Trichoderma 5-10 gm/kg. seed (before transplanting) Chloropyriphos 3gm/kg seed. Pseudomonas flourescens0.5% W.P. 10 gm/kg. Chillies Anthracnose spp. Damping off Seed treatment with Trichoderma viride 4g/kg, Carbandazim @ 1g/100 gm seed.
  • 38. Tomato Soil borne infection of fungal disease Early blight Damping offWilt T. viride @ 2 gm/100gm seed. Captan 75 WS @ 1.5 to 2.0 gm a.i./litre for soil drenching.Pseudomonas fluorescens and V. clamydosporium @ 10gm/kg as seed dresser. Crucifero us veget ables (Cabbage Cauliflo wer, Broccoli, Knol- khol, radish) Soil / Seed borne diseas es (Damping off)Root knot nematode Seed treatment with Trichoderma viridi @ 2 g / 100 g seeds Captan 75% WS @ 1.5 to 2.5 gm a.i./litre for soil drenching.Pseudomonas fluorescens and Verlicillium clamydosporium @ 10gm/kg seed as seed dresser