PRRSV Evolution Emerging Strains

PRRSV Evolution and New, Emerging Strains
11/20/2015
These producers are willing to share their premises IDs and
pathogen status in the interests of national disease control.
Regional Projects:
N212 MN, PA, and SE IA
Michael Murtaugh
Xiong Wang
Mohammad Alkhamis
Bob Morrison
Andres Perez
University of Minnesota

There are Two General Types of PRRSV
Type 1
(European)
Type 2
(North American)
ORF5 Dendrogram

PRRSV2/USA/MN17A/2012
PRRSV2/USA/MN17B/2013
PRRSV2/USA/MN16/2011
PRRSV2/USA/MN2/2012
PRRSV2/USA/1-2-3Yeske
PRRSV2/Illinois/XW073/2011/ORF5PRRSV2/Indiana/XW071/2010/ORF5PRRSV2/USA/IA12/2012
PRRSV2/USA/NADC30/2008
PRRSV2/CHN/HENAN-HEB
PRRSV2/Indiana/XW087/2013/ORF5
PRRSV2/USA/MN6/2012
PRRSV2/USA/MN7/2012
PRRSV2/USA/MN5/2012
PRRSV2/USA/MN4/2012
PRRSV2/Iowa/XW091/2013/ORF5
PRRSV2/USA/BI-Sample2
PRRSV2/USA/AL27
PRRSV2/USA/EP37
PRRSV2/USA/MN3/2012
PRRSV2/Michigan/XW090/2011/ORF5
PRRSV2/KOR/CA-2/2013
PRRSV2/USA/Iowa21/2015/ORF5
PRRSV2/USA/Hawkeye7_Before
PRRSV2/USA/MN184A
PRRSV2/USA/Biss_After
PRRSV2/USA/Hawkeye4_After
IAF-Klop CAN L1
PRRSV2/USA/NC174/2015/ORF5
PRRSV2/USA/MN1/2012PRRSV2/USA/Hawkeye2_Before
PRRSV2/USA/QUAL2_After
PRRSV2/Minnesota/XW095/2007/ORF5
PRRSV2/USA/BonHomme(Spronk)
PRRSV2/USA/JA1262
02SP3THAL1
98-3298L1
IAF-EXP91CANL1
FF41-7-2
HU1021012
PRRSV2/CHN/QY2010/2010
PRRSV2/CHN/SH1211/2012
FJ-1CHNL3
Jyn1JPNL5.1
A11-57151-5-2
PA8L5.1
MLVPRRS2-5-2
U-USPOLL5.1
LU534AUTL5.1
PRRSV2/Illinois/XW051/2011/ORF5PRRSV2/USA/ISU-P
PRRSV2/DEN/DK-2010-10-4-1/2010
PRRSV2/CHN/HZ-31/2012
PRRSV2/DEN/DK-2004-2-1/200430352-3MIL5.2
ISU55L5.2
PRRSV2/KOR/LMY/2002
NADC-8L5.2
PRRSV2/USA/MtEcho
34075NEL6
PrimePacNeb-1L7
5591 NCL9
PRRSV2/USA/CleenyWest
PRRSV2/USA/JZ8
PRRSV2/USA/JZ2
MN30100 1-4-2
2000-5424 L917198-6 L9
PRRSV2/Indiana/XW092/2013/ORF5PRRSV2/USA/NC16845/2006PRRSV2/Indiana/XW083/2010/ORF5
02-01676 L9
2000-52502 L8
98-6470-1 L9
NADC20
ATP PRRS 1-4-2
PRRSV2/USA/JA142/2004
98-31701-1 L8
Fostera PRRS 1-3-2
24901 GTM L8
PRRSV2/USA/E32
SDSU-73 L8
CH-1a L8
PRRSV2/CHN/Em2007/2007
PRRSV2/CHN/SHB/2005
PRRSV2/CHN/GD3/2005
PRRSV2/CHN/HB-1/2001
PRRSV2/CHN/HK4/2003
PRRSV2/CHN/NVDC-GD2-2011/2011
PRRSV2/VIE/SRV07/2007
PRRSV2/CHN/NVDC-JS2-2011/2011
PRRSV2/CHN/09HUB7/2009
PRRSV2/VIE/07QN/2007
PRRSV2/CHN/09HUN1/2009
PRRSV2/CHN/09BJ/2009
PRRSV2/CHN/09HEB/2009
PRRSV2/LAO/10-LW8-1/2010
PRRSV2/CHN/JX
PRRSV2/CHN/GD-2011/2011PRRSV2/VIE/10-10QN/2010
0.02
Lineage 1
Lineage 2Lineage 5.1
Lineage 5.2
Lineage 6,7
Lineage 9
Lineage 8
Type 2 PRRSV
Diversity ORF5

PRRSV2/USA/MN2/2012
PRRSV2/Illinois/XW073/2011/ORF5PRRSV2/Indiana/XW071/2010/ORF5PRRSV2/USA/IA12/2012
PRRSV2/USA/MN6/2012
PRRSV2/USA/MN7/2012
PRRSV2/USA/MN5/2012
PRRSV2/USA/MN4/2012
PRRSV2/Iowa/XW091/2013/ORF5
PRRSV2/USA/AL27
PRRSV2/USA/EP37
PRRSV2/USA/MN3/2012
PRRSV2/USA/Iowa21/2015/ORF5
PRRSV2/USA/MN184A
IAF-Klop CAN L1
PRRSV2/USA/NC174/2015/ORF5
PRRSV2/USA/MN1/2012PRRSV2/USA/Hawkeye2_Before
PRRSV2/Minnesota/XW095/2007/ORF5
PRRSV2/USA/JA1262
02SP3THAL1
98-3298L1
IAF-EXP91CANL1
FF41-7-2
HU1021012
FJ-1CHNL3
Jyn1JPNL5.1
A11-57151-5-2
PA8L5.1
MLVPRRS2-5-2
U-USPOLL5.1
LU534AUTL5.1
PRRSV2/Illinois/XW051/2011/ORF5PRRSV2/USA/ISU-P
PRRSV2/DEN/DK-2010-10-4-1/2010
PRRSV2/DEN/DK-2004-2-1/200430352-3MIL5.2
ISU55L5.2
PRRSV2/KOR/LMY/2002
NADC-8L5.2
PRRSV2/USA/MtEcho
34075NEL6
PrimePacNeb-1L7
5591 NCL9
PRRSV2/USA/JZ8
PRRSV2/USA/JZ2
MN30100 1-4-2
2000-5424 L917198-6 L9
PRRSV2/Indiana/XW092/2013/ORF5PRRSV2/USA/NC16845/2006PRRSV2/Indiana/XW083/2010/ORF5
02-01676 L9
2000-52502 L8
98-6470-1 L9
NADC20
ATP PRRS 1-4-2
98-31701-1 L8
Fostera PRRS 1-3-2
24901 GTM L8
PRRSV2/USA/E32
SDSU-73 L8
CH-1a L8
PRRSV2/CHN/SHB/2005
PRRSV2/CHN/GD3/2005
PRRSV2/CHN/HB-1/2001
PRRSV2/CHN/HK4/2003
PRRSV2/CHN/JX
PRRSV2/CHN/GD-2011/2011PRRSV2/VIE/10-10QN/2010
0.02
Lineage 1
Lineage 2Lineage 5.1
Lineage 5.2
Lineage 6,7
Lineage 9
Lineage 8
1-8-4
1-7-4
2-5-2
1-4-2
1-4-2
HP PRRS
Type 2 PRRSV
Diversity ORF5

PRRSV2/CHN/DC/2010
PRRSV2/CHN/GX10-48/2006
PRRSV2/CHN/10-10BJ-1/2010
PRRSV2/CHN/10-10SD/2010PRRSV2/CHN/09JS/2009PRRSV2/CHN/09HEN1/2009PRRSV2/CHN/GD-2011/2011PRRSV2/CHN/10HD-GD/2010PRRSV2/CHN/10-10JX/2010
PRRSV2/CHN/BJ1102/2011
PRRSV2/CHN/WUH3/2008
PRRSV2/CHN/09DB2/2009
PRRSV2/CHN/10-10JL/2010
PRRSV2/CHN/11GZ-GD/2011
PRRSV2/CHN/SX-1/2009
PRRSV2/CHN/09SD/2009
PRRSV2/CHN/09SC/2009
PRRSV2/CHN/SX2009/2009
PRRSV2/CHN/SD0901
PRRSV2/CHN/ZP-1/2009
PRRSV2/CHN/BJ/2007
PRRSV2/CHN/XJu-1/2012
PRRSV2/CHN/BJsy06/2006
PRRSV2/CHN/NX06
PRRSV2/CHN/SD16/2012
PRRSV2/CHN/07HEN/2007
PRRSV2/CHN/HUN2/2006
PRRSV2/CHN/JXM80/2008
PRRSV2/CHN/HEB1/2006
PRRSV2/CHN/SD-CXA/2009
PRRSV2/CHN/GX1002/2010
PRRSV2/CHN/JL-0412/2012
PRRSV2/CHN/KP
PRRSV2/CHN/YD/2009
PRRSV2/VIE/10-10QN/2010
PRRSV2/CHN/LN/2006
PRRSV2/CHN/HV
PRRSV2/CHN/AH0701/2007PRRSV2/CHN/GDQJ/2007PRRSV2/CHN/08SDWF/2008
PRRSV2/CHN/JXA1/2006
PRRSV2/CHN/Henan-A8/2013
PRRSV2/CHN/SDA3/2011
PRRSV2/CHN/CWZ-1-F3/2008
PRRSV2/CHN/CG/2007
PRRSV2/CHN/GDQY/2007
PRRSV2/CHN/NJ-1106/2012
PRRSV2/CHN/NT0801/2008
PRRSV2/CHN/GD3/2005
PRRSV2/CHN/HK4/2003
PRRSV2/CHN/SHB/2005
PRRSV2/CHN/HK12/2004
PRRSV2/CHN/HB-1(sh)/2001
PRRSV2/CHN/LN1101
PRRSV2/CHN/CH-1a/1999
PRRSV2/CHN/CH-1R
PRRSV2/CHN/GM2/2011
PRRSV2/USA/MN30100
PRRSV2/USA/NC16845/2006
PRRSV2/USA/Ingelvac_ATP
PRRSV2/USA/INGELVAC_ATP
PRRSV2/USA/MtEcho
PRRSV2/USA/NVSL97-7985/1997
PRRSV2/USA/SDSU73PRRSV2/USA/E32
PRRSV2/USA/JZ1
PRRSV2/USA/JZ8
PRRSV2/USA/JZ2
PRRSV2/USA/FF4_After
PRRSV2/USA/FF3_Before
PRRSV2/USA/Flagship_Before
PRRSV2/USA/Prime_Pac/1996
PRRSV2/KOR/LMY/2002
PRRSV2/USA/ISU-P
PRRSV2/DEN/DK-2004-2-1/2004
PRRSV2/DEN/DK-2008-10-1-3/2008
PRRSV2/DEN/DK-2003-2-3/2003PRRSV2/CHN/HN1
PRRSV2/DEN/DK-2010-10-4-1/2010
PRRSV2/DEN/DK-2011-88005-A8-Pl/2011
PRRSV2/DEN/DK-2012-01-11-3/2012
PRRSV2/USA/QUAL1_Before
PRRSV2/USA/1784(IA)
PRRSV2/DEN/DK-1997-19407B/1997
PRRSV2/KOR/PL97-1/1997
PRRSV2/USA/VR2332/1995
PRRSV2/USA/SD95-21/1995
PRRSV2/USA/VR2332
PRRSV2/CHN/YN-2011/2011
PRRSV2/USA/MLV
PRRSV2/CHN/GS2004/2004
PRRSV2/CHN/SD1-100
PRRSV2/CHN/DY/2007
PRRSV2/HUN/Hungary102/2012
PRRSV2/USA/Biss_Before
PRRSV2/USA/Lewis_After
PRRSV2/USA/Lewis_Before
PRRSV2/USA/MFF_After
PRRSV2/USA/MN184B
PRRSV2/USA/MN/MN184C
PRRSV2/USA/MN184APRRSV2/USA/FF2_AfterPRRSV2/USA/Flagship_AfterPRRSV2/USA/Iowa21/2015 15036 lfv_free
PRRSV2/USA/North_Carolina59/2015 15059 lfv_free
PRRSV2/USA/K9/2009
PRRSV2/CHN/HENAN-XINX/2013
PRRSV2/USA/JA1262
PRRSV2/USA/IA12/2012
PRRSV2/USA/IL8/2012
PRRSV2/USA/MN2/2012
PRRSV2/USA/MN1/2012
PRRSV2/USA/MN3/2012
PRRSV2/USA/MN7/2012
PRRSV2/USA/AL27
PRRSV2/USA/EP37
PRRSV2/USA/MN4/2012
PRRSV2/USA/K10/2010
PRRSV2/USA/MN5/2012
0.02
Type 2 PRRSV Diversity
Whole Genome
HP PRRS
Ingelvac MLV
Reisolates Conclusions: Genbank sampling is
highly biased. No naming system.

Mlu I HincII Sac II
1 8 4
1 18 2
2 5 2
1 26 2
ORF5
0 100 200 300 400 500 600
Here are some common PRRSV RFLP types

Table 1. PRRS virus Restriction Fragment Length Polymorphism (RFLP) cut sites
MluI Digestion = A^CGCGT HincII Digestion = GT(CT)^(AG)AC SacII Digestion = CCGC^GG
RFLP # Cut Sites RFLP # Cut Sites RFLP # Cut Sites
1 - 1 - 1 -
2 408 2 88 2 24
3 336 3 219 3 555
4 532 4 88, 219 4 24, 555
5 360 5 24, 582
6 88, 360 6 24, 310
7 88, 219, 360 7 24, 357
8 88, 219, 381 8 24, 310, 555
9 219, 515
10 219, 360
11 360, 515
12 219, 381
13 88, 219, 508
14 88, 291
15 88, 219, 291, 381, 515
16 88, 381
17 88, 502
18 88, 508
19 381, 508
20 360, 596
21 88, 219, 360, 381
22 219, 502
23 219, 360, 381
24 88, 381, 508
25 88, 219, 596
26 508
27 515
28 88, 508, 523
29 88, 172, 219
30 381
31 88, 135, 219
32 88, 219, 360, 515
33 88, 219, 515
34 291, 360
35 219, 523
36 88, 219, 360, 596
37 219, 508
38 219, 502, 523
39 502
40 88, 219, 291
41 88, 502, 515
42 94, 219
43 88, 219, 360, 523
44 88, 219, 558
45 88, 219, 381, 596
46 88, 360, 508
47 219, 596
48 88, 135
49 88, 509
50 94,508
51 88, 219, 282
52 172, 360
53 88, 360, 523
54 103, 360
55 88, 219, 360, 508
Year 2010
1-4
1-55
1-8

Table 1. PRRS virus Restriction Fragment Length Polymorphism (RFLP) cut sites
MluI Digestion = A^CGCGT HincII Digestion = GT(CT)^(AG)AC SacII Digestion = CCGC^GG
RFLP # Cut Sites RFLP # Cut Sites RFLP # Cut Sites
1 - 1 - 1 -
2 408 2 88 2 24
3 336 3 219 3 555
4 532 4 88, 219 4 24, 555
5 360 5 24, 582
6 88, 360 6 24, 310
7 88, 219, 360 7 24, 357
8 88, 219, 381 8 24, 310, 555
9 219, 515
10 219, 360
11 360, 515
12 219, 381
13 88, 219, 508
14 88, 291
15 88, 219, 291, 381, 515
16 88, 381
17 88, 502
18 88, 508
19 381, 508
20 360, 596
21 88, 219, 360, 381
22 219, 502
23 219, 360, 381
24 88, 381, 508
25 88, 219, 596
26 508
27 515
28 88, 508, 523
29 88, 172, 219
30 381
31 88, 135, 219
32 88, 219, 360, 515
33 88, 219, 515
34 291, 360
35 219, 523
36 88, 219, 360, 596
37 219, 508
38 219, 502, 523
39 502
40 88, 219, 291
41 88, 502, 515
42 94, 219
43 88, 219, 360, 523
44 88, 219, 558
45 88, 219, 381, 596
46 88, 360, 508
47 219, 596
48 88, 135
49 88, 509
50 94,508
51 88, 219, 282
52 172, 360
53 88, 360, 523
54 103, 360
55 88, 219, 360, 508
March 30
2015 1-4 1-114 1-12
November 17
2015 1-5 1-125 1-13

RFLP pattern does not identify
genetic relationships
JZ1 None 142
CleenyWest None 132
JZ2 None 142
JZ8 None 142
E32 None 124
NVSL AF325691 144
Mt.Echo None 132
JA142 AY424271.1 142
NVSL 97 AY545985 142
Juan ATP EF532801.1 142
JZ4 None 142
Juan MN30100 EF536000.1 142
CH1a AY032626 141
P129 AF494042 132
Fostera NONE 132
FlagshipBefore EF532805.1 172
Flagship3(FF3) EF532808.1 172
Flagship4(FF4) EF532809.1 172
Hawkeye4Before EF532813.1 144
Quality2(After) EF536002.1 144
Juan VR2332 EF536003.1 262
Juan ISU EF532816.1 152
16244b AF046869 152
Quality1(Before) EF536001.1 252
1784(IA) None 252
PA8 AF176348 252
MLV AF066183 252
Hawkeye7Sow1 DQ459471 252
VR2332a AY150564.1 252
VR2332b PRU87392 252
AL27 None 144
EP37 None 144
BI-Sample1 None 144
BI-Sample2 None 144
1-2-3Yeske None 123
K10(Kingston2010) None 144
K12(B)(Kingston2012) None 144
Hawkeye2After EF532810.1 1182
Hawkeye2Before EF532811.1 144
JA1262 None 1262
BonHomme(Spronk) None 1182
Hawkeye4After(H4New) EF532812.1 184
Hawkeye7sow2(After) EF532814.1 184
Hawkeye7Dlab(Before) EF532815.1 184
MFFOLD(After) EF532819.1 184
BissonetteAfter(BA) EF532802.1 184
BissonetteBefore(BB) EF532803.1 184
LewisAfter(WindmillAfter) EF532817.1 184
LewisBefore(WindmillBefore) EF532818.1 184
FlagshipAfter EF532804.1 184
FlagshipFitzsimmons2(FF2) EF532807.1 184
FlagshipFitzsimmons1(FF1) EF532806.1 184
MFFNEW(Before) EF535999.1 184
Hungary2
0.02
1-4-4 RFLP shows up in 3
widely divergent families

0%
10%
20%
30%
40%
50%
60%
70%
80%
90%
100%
2001 2002 2003 2004 2005 2006 2007 2008 2009 2010
percentageofPRRSvirussequencesfittinganRFLPpattern
Figure 1. Distribution of major PRRS virus RFLP cut patterns from
University of Minnesota Veterinary Diagnostic Laboratory
case submissions sequenced from 2001-2010
2 5 2
ATP
1 4 2
1 8 4
1 4 4
1 2 2
1 18 2
1 26 2
Ecology of PRRSV

0.05
6774 PRRSV isolates
1998-2015
Type 1
N=107 (1.6%)
15 years of PRRSV
Diagnostic
Sequencing in USA
5 Large US Swine
Producers
98.4% are Type 2
Conclusions:
 Large outbreaks with little
genetic change are frequent
 Control programs are effective
 Genbank does not show reality

PRRSV2/CHN/DC/2010
PRRSV2/CHN/10-10FUJ-1/2010
PRRSV2/CHN/10-10GX-5/2010
PRRSV2/CHN/10-10BJ-1/2010PRRSV2/CHN/10-10SD/2010PRRSV2/CHN/09JS/2009PRRSV2/CHN/BB0907/2009PRRSV2/CHN/JN-HS2008PRRSV2/CHN/09HEN1/2009PRRSV2/CHN/10HD-GD/2010PRRSV2/CHN/GD-2011/2011
PRRSV2/CHN/10-10HEB-3/2010
PRRSV2/CHN/10-10JX/2010
PRRSV2/CHN/JX
PRRSV2/CHN/HLJHL/2009
PRRSV2/CHN/09DB2/2009
PRRSV2/CHN/10-10JL/2010
PRRSV2/CHN/Henan-1
PRRSV2/CHN/SY0608/2006
PRRSV2/CHN/SCwhn09CD/2009
PRRSV2/CHN/SX-1/2009
PRRSV2/CHN/09SD/2009
PRRSV2/CHN/ZP-1/2009
PRRSV2/CHN/SD0901
PRRSV2/CHN/09SC/2009
PRRSV2/CHN/SX2009/2009
PRRSV2/CHN/Henan-A8/2013
PRRSV2/CHN/XJu-1/2012
PRRSV2/CHN/BJsy06/2006
PRRSV2/CHN/NX06
PRRSV2/CHN/BJ/2007
PRRSV2/CHN/11GZ-GD/2011
PRRSV2/CHN/XH-GD
PRRSV2/CHN/JSyx/2006
PRRSV2/CHN/GDBY1/2008
PRRSV2/CHN/SD16/2012
PRRSV2/CHN/07HEN/2007
PRRSV2/CHN/07HEBTJ/2007
PRRSV2/CHN/07NM/2007
PRRSV2/CHN/Jiangxi-3
PRRSV2/CHN/HUN2/2006
PRRSV2/CHN/GD/2006
PRRSV2/CHN/JXM80/2008
PRRSV2/CHN/HEB1/2006PRRSV2/CHN/SD-CXA/2009PRRSV2/CHN/NVDC-JS2-2011/2011
PRRSV2/CHN/GX1002/2010
PRRSV2/CHN/JL-0412/2012
PRRSV2/CHN/KPPRRSV2/CHN/YD/2009
PRRSV2/VIE/10-10QN/2010
PRRSV2/CHN/LN/2006
PRRSV2/CHN/HV
PRRSV2/CHN/SHH/2006
PRRSV2/CHN/SY0909/2009
PRRSV2/CHN/AH0701/2007
PRRSV2/CHN/GDQJ/2007
PRRSV2/CHN/TP/2006
PRRSV2/CHN/HPBEDV
PRRSV2/CHN/JXA1/2006
PRRSV2/CHN/08SDWF/2008
PRRSV2/CHN/SDA3/2011
PRRSV2/CHN/HUN4
PRRSV2/CHN/HN-HW/2006
PRRSV2/CHN/GD/2007
PRRSV2/CHN/CWZ-1-F3/2008
PRRSV2/CHN/08HuN/2008
PRRSV2/CHN/CG/2007
PRRSV2/CHN/GDQY/2007
PRRSV2/CHN/NJ-1106/2012
PRRSV2/CHN/NT0801/2008
PRRSV2/CHN/GD3/2005
PRRSV2/CHN/HK4/2003
PRRSV2/CHN/SHB/2005
PRRSV2/CHN/HK12/2004
PRRSV2/CHN/HB-1(sh)/2001
PRRSV2/CHN/LN1101
PRRSV2/CHN/CH-1a/1999
PRRSV2/CHN/CH-1R
PRRSV2/CHN/GM2/2011
PRRSV2/CHN/QYYZ/20011
PRRSV2/USA/MN30100PRRSV2/USA/NC16845/2006
PRRSV2/USA/INGELVAC_ATP
PRRSV2/USA/MtEchoPRRSV2/USA/NVSL97-7985/1997PRRSV2/USA/SDSU73
PRRSV2/USA/E32
PRRSV2/USA/JZ1
PRRSV2/USA/JZ8
PRRSV2/USA/JZ2
PRRSV2/USA/Flagship_Before
PRRSV2/USA/Prime_Pac/1996
PRRSV2/KOR/LMY/2002PRRSV2/USA/ISU-P
PRRSV2/DEN/DK-2004-2-1/2004
PRRSV2/DEN/DK-2008-10-1-3/2008
PRRSV2/DEN/DK-2003-2-3/2003
PRRSV2/CHN/HN1
PRRSV2/DEN/DK-2011-88005-A8-Pl/2011
PRRSV2/DK/DK-2010-10-13-1/2010
PRRSV2/DEN/DK-2010-10-4-1/2010
PRRSV2/DEN/DK-2012-01-11-3/2012
PRRSV2/USA/QUAL1_Before
PRRSV2/USA/1784(IA)
PRRSV2/DEN/DK-1997-19407B/1997
PRRSV2?/KOR/PL97-1/1997
PRRSV2/KOR/PL97-1-LP1/1997
PRRSV2/CHN/PRRSV03
PRRSV2/USA/VR2332/1995
PRRSV2/USA/A2MC2
PRRSV2/USA/VR2332a
PRRSV2/USA/SD95-21/1995
PRRSV2/USA/VR2332
PRRSV2/CHN/YN-2011/2011
PRRSV2/CHN/CC-1
PRRSV2/USA/MLV
PRRSV2/CHN/GS2004/2004
PRRSV2/CHN/BJ-4/2000
PRRSV2/CHN/SD1-100
PRRSV2/CHN/DY/2007
PRRSV-2_Hungary_102_2012PRRSV2/KOR/CA-2/2013
PRRSV2/USA/Biss_Before
PRRSV2/USA/Lewis_After
PRRSV2/USA/Lewis_Before
PRRSV2/USA/MFF_AfterPRRSV2/USA/MN184BPRRSV2/USA/MN/MN184CPRRSV2/USA/MN184A
PRRSV2/USA/MFF_Before
PRRSV2/USA/Flagship_After
PRRSV2/USA/K9/2009
PRRSV2/CHN/HENAN-XINX/2013
PRRSV2/USA/JA1262
PRRSV2/USA/IA12/2012
PRRSV2/USA/IL8/2012
PRRSV2/USA/MN2/2012
PRRSV2/USA/MN1/2012
PRRSV2/USA/MN3/2012
PRRSV2/USA/MN6/2012
PRRSV2/USA/MN7/2012
PRRSV2/USA/AL27
PRRSV2/USA/EP37
PRRSV2/USA/MN4/2012
PRRSV2/USA/MN5/2012PRRSV2/USA/K10/2010
PRRSV2/USA/K11/2011
PRRSV2/USA/K12/2012
0.02
Chinese
HP-PRRSV
2006-2009
>99% identical
2006-2011
>98% identical

Type 2 PRRSV
Type 1 PRRSV One System
160 ORF5 sequences
+ References

Apr 1, 2012–Jun 17, 2013
1-4-4
1-4-4
1-4-4
1-4-4
1-4-4 1-4-4
1-4-4 Outbreaks

May 21, 2014–March 31, 2015
1-7-4
1-7-4
1-7-4
1-7-4
1-7-4 Outbreaks

May 21, 2014–March 31, 2015
1-7-4
1-7-4
1-7-4
1-7-41-4-41-6-4
1-6-4
1-7-3
1-21-4
1-7-4 Outbreaks
Same virus – Multiple RFLP patterns

1-7-4-PRRSV Family Story
241 1-7-4 family
Jan 2014 -Mar 2015
0
.0
5
6774 sequences
1998-2015

Anatomy of Disaster
1-7-4 Outbreaks Investigation
Jun 2014
Jun 2014
May 2014
Apr 2014
Jun 2014
Sep 2014
Dec 2014
Jan 2015
Jan 2015

IA-N 20150224 Q
IA-N 20150309 W
IA-N 20150223 O
IA-N 20150128 I
IA-N 20150128 H
IA-N 20150204 K
IA-N 20150306 S
SO 20150206 M
NC 20140520 174
OH-20140408-A
IA-N 20140923 D
IA-N 20150105 G
IA-N 20141218 E
IA-N 20150102 F
MN-20140630-C
NC-20150223-P
NC-20150129-J
NC-20150310-U
NC-20150309-T
NC-20150310-V
IA E 20140601 B
IA-S-20150303-R
IA-S-20150206-L
IA-S-20150220-N
J (1-18-2) 2008
D13-046045 1-73-2
AS (1-16-3) 2013
PRRSV2/USA/MN184A
2009012092 1-18-4
A13-13598 1-4-4
ISU55 L5.2
PrimePac(2)
02-01676 L9
NADC20
PRRSV2/USA/E32
CH-1a CHN L8
VIE/10-10QN/2010
JXAI CHN L8
IA-N20150224Q
IA-N20150309W
IA-N20150223O
IA-N20150128I
IA-N20150128H
IA-N20150204K
IA-N20150306S
SO20150206 M
NC 20140520 174
OH-20140408-A
IA-N 20140923 D
IA-N 20150105 G
IA-N 20141218 EIA-N20150102F
MN-20140630-C
NC-20150223-P
NC-20150129-J
NC-20150310-U
NC-20150309-T
NC-20150310-V
IAE20140601B
IA-S-20150303-R
IA-S-20150206-L
IA-S-20150220-N
J(1-18-2)2008
D13-0460451-73-2
AS(1-16-3)2013PRRSV2/USA/MN184A2009012092 1-18-4
A13-13598 1-4-4
ISU55 L5.2
PrimePac(2)
02-01676 L9
NADC20
PRRSV2/USA/E32
CH-1aCHNL8
VIE/10-10QN/2010
JXAICHNL8
0.02
The Devil’s Spawn
Distant Offspring of the 1-8-4 Family

nsp2 Deletions are Unremarkable
No other distinctive features are apparent

Signal Peptide Ectodomain 1 TM Ectodomain 2
Proteins are Essentially the Same
Envelope Glycoprotein 5 (GP5)
No evidence of directed evolution or selection during outbreak

Bayesian Phylodynamics
Skyline Plot of Estimated Population Size over Time
Bayesian phylogenetic methods incorporate associated epidemiological
information (e.g. geographic location, time of isolation)

History of Spread Between Systems

Outbreaks are Concentrated in Sow Farms

Shields and Mathews (2003). Interstate Livestock Movements. USDA-Economic Research Service Outlook Report
www.ers.usda.gov/publicatons/ldp/jun03/ldpm10801/ldpm10801.pdf.
A B
Swine outflows from North Carolina Swine inflows to Minnesota
Interstate pig movements in the U.S.A. in 2001

Take Home Messages
Large-scale PRRS outbreaks in the history of type 2 PRRSV
Genetic change during outbreaks is limited
Bayesian phylodynamics helps understand disease spread
PRRSV goes where pigs go

PRRSV Evolution Emerging Strains

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