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Optimization of process parameters
1. By:
Sumit Pannu
Roll No. : 15001511009
M.Tech-3rd
Sem
Optimization of process parameters for maximum
production of Tannase using Plackett-Burman method
Department of Biomedical Engineering
DCRUST-Murthal
Under the Guidance of:
Mr. Saravjeet Singh
Asst. Prof., Deptt. of BME
D.C.R.U.S.T.
Sonipat-Haryana
2. Table of contentsof contents
โข Introduction
โข Plackett-Burman method
โข Tannase production
โข Uses of Tannase
โข Work plan
โข References
3. Introduction
โข We get the significant factors for maximum production
of Tannase using Plackett-Burman method .
โข Tannase has great commercial significance in the feed,
drink and medical industries.
โข Tannase is an enzyme catalyzing the hydrolysis of the
tannic ( C76H52O46 ) , that is present in many different
parts of plants, to produce glucose and gallic acid
(C6H2(OH)3COOH) or Tri-Hydroxy-Benzoic Acid.
5. Plackett โBurman MethodPlackett โBurman Method
โข Developed in 1946 by statisticians Robin L. Plackett &
J.P. Burman.
โข It is an efficient screening method to identify the
active factors using as few experimental runs as
possible.
โข With an 8-Run Plackett-Burman design, we can look at
up to 7 factors; with a 12-Run design, up to 11 factors
and with a 16-Run design up to 15 factors.
6. When to Use Plackett-Burman Design ?
It is particularly helpful to use Plackett-Burman design:
โขIn screening of process parameters
โขIn two-level multi-factor experiments
โขWhen there are more than four factors (if there are between
two to four variables, a full factorial can be performed)
โขTo economically detect large main effects
For N = 12, 20, 24, 28 and 36 (where N = the number of
experiments)
7. Analysis of Experiment Results
โข Once the experiment is run and the samples measured, the
data from the experiment are used to calculate the effects
and determine the statistical significance of those effects
by two way ANOVA(analysis of variance) for different
factors and different levels.
โข There is no way to know if the effects are due to changes
made in the factor levels or due to common cause
variation in the process unless their statistical significance
is tested.
8. Production of Tannase
โข Submerged fermentation: It is the process of producing
enzyme(tannase) with the help of micro-organism.
Micro-organism(bacteria erwinia) breakdown the
nutrients and release desired amount of enzyme in the
solution.
โข Micro-organism seeded in liquid(tannic acid ,other
sources of tannic acid,H2O,KCLand NaNO3 ) for some
time(20-24 hours for tannase) to produce tannase enzyme.
โข Enzyme activity โ moles of substrate converted per unit
time (unit per ml).
9. Process parameters for tannase productionProcess parameters for tannase production
โข PH - 2-8
โข Temperature - 30-35ยฐ c
โข Incubation time - 20-24 hours
โข Metal ions - k++
and Na++
โข Nitrogen and carbon sources
10. Substrate for Tannase productionSubstrate for Tannase production
Substrate Tannase activity
(Unit/ml)
Pomegranate peel 79.5
Grape seed 40
Pine bark 64
Oak bark 46
11. Uses of TannaseUses of Tannase
โข Bioconversion of tannic acid by enzyme tannase to
produce gallic acid(Tri-hydroxy benzoic acid).
โข Gallic acid is used for preparation of :
- antibacterial drugs
- vitamin supplements
- fruit juice
- coffee flavoured soft drink
โข Gallic acid has anti-cancer properties(prevent cellular
mutations) and anti-fungal properties.
12. Work planWork plan
Work Expected time
Experimental work 2-3 months
Statistical work 15-20 days
Other 10-15 days
13. ReferencesReferences
[1].Sahira N. Muslim,Alaa N. Mahammed,Hadeel K. Musafer,Israa
M. S. AL_Kadmy,and Shatha A. Shafiq, Sraa N. Muslim โDetection
of the Optimal Conditions for Tannase Productivity and Activity by
Erwinia Carotovoraโ, Journal of Medical and Bioengineering Vol. 4,
No. 3, June 2015.
[2]. Swaran Nandini1, Nandini KE2 and Krishna Sundari S2, โFood
and Agriculture Residue (FAR): A Potential Substrate for Tannase
and Gallic Acid Production using Competent Microbesโ , Nandini,
et al., J Bioproces Biotech 2014.
[3]. QualityTrainingPortal6,2000-2016 Resource Engineering, Inc.
All rights reserved.
[4]. Manikandan jayakumar,When and How to Use Plackett-Burman
Experimental Design ยฉ Copyright iSixSigma 2000-2016.