“Lenoil” SHP is a biological product (oil destructor) designed to eliminate the effects of oil spills and oil products on soils and water bodies.

1. LENOIL
Biological product for the
elimination of oil pollution
of soil and water surfaces

2. The largest enterprises, which mining oil and
produce in large volumes petrochemicals,
inevitably waste substances which contain
hazardous, including:
•ground contaminated with petroleum products,
•sludges containing crude oil,,
•soil contaminated with crude oil,
At present, the problem of their utilization is
urgent, and developed technologies are not
excellent. We have designed technological
methods of biological treatment of objects,
contaminated with oil and petroleum products
using the biological product "LENOIL"

3. The purification technology of oil pollution is based on the use of microorganisms
actively utilizing petroleum hydrocarbons. Preparation "Lenoil" allows you to
recreate the natural biological processes of oil-contaminated soil region.

4. • The biological product is a
consortium of microorganisms
Bacillus brevis and Arthrobacter
species

5. Oxidizing ability of
microorganisms of "Lenoil"
biological product
№ Substrate
Oxidative activity mg CO2/g of substrate
Athrobacter, sp Bacillus brevis Lenoil
1 Oil 84 112 144
2 Diesel fuel 60 138 163
3 Lubricating oil
99 104
120
4 Benzene 85 69 96
5 Phenol 36 30 43
6 Paraffin 11 127 77
7 Ceresin 14 18 21
8 Naphthalene
88 53
92
9 Heptane 77 113 170
10 Dean 33 104 140
11 Undecane 94 161 175
12 Dodecane 116 153 181
13 Hexadecanol
91 215
219
14 Cyclohexane
99 90
110
15 Toluene 93 81 89
16 O-xylen 76 92 100
17 Isopropanol
62 71
89
18 Sunflower oil
104 116
128
19 Beef-oil 148 173 176
20 Pork fat 63 172 170

6. – After the expiry of biological product, in the
soils treated from oil, remains easily digestible
vegetable organic residue and
environmentally friendly neutral oil oxidation
products, contributing to the development of
natural microflora ecosystem.

7. As a result of biological product LENOIL on the water
surface, within a month the oil becomes foamy
structure of brown color with a specific smell, later,
settling to the bottom as sludge, which in turn, is
processed plankton ponds

8. • The most effective way of product
application is to spray the soil with
it in the form of suspension with
mineral additives. Furthermore, as
one of the components used
supplement containing bioactive
substances that enhance the
activity of microorganisms, oil
destructors.

9. • Many years of experience in
remediation works show
that in summer season the
level of oil destruction in the
soil reaches 85%.

10. Lenoil effectiveness in comparison withLenoil effectiveness in comparison with
other biological productsother biological products
Product Name
Degree of pollution, %
The level of oil biodegradation
soil turf
Lenoil
10,0 85,3 84,3
20,0 70,2 66,9
30,0 47,9 45,0
Devoroil
10,0 80,6 79,6
20,0 61,8 59,4
30,0 44,2 34
Destroil
10,0 72,9 73,0
20,0 56,4 58,9
30,0 26,8 23,6
Bacispecin
10,0 63,3 57,8
20,0 47,8 38,0
30,0 31,6 30,0

11. 
 Developed the technology of recycling waste hydrocarbon-petrochemical industryDeveloped the technology of recycling waste hydrocarbon-petrochemical industry
with the ability to use complex microbiological preparations "Lenoyl", "azole" andwith the ability to use complex microbiological preparations "Lenoyl", "azole" and
"Batsispetsin" and structurants (typical black soil, sawdust, phosphogypsum). The"Batsispetsin" and structurants (typical black soil, sawdust, phosphogypsum). The
implementation of the proposed technology significantly reduces the amount ofimplementation of the proposed technology significantly reduces the amount of
residual hydrocarbons in recultivated substrates for one growing season.residual hydrocarbons in recultivated substrates for one growing season.
 The biotechnology successfully tested in field conditions of the remediation works atThe biotechnology successfully tested in field conditions of the remediation works at
the site of "Orsknefteorgsintez", as well as on the territory of the oil spill in the OGPUthe site of "Orsknefteorgsintez", as well as on the territory of the oil spill in the OGPU
"Buguruslanneft" "Orenburgneft.“"Buguruslanneft" "Orenburgneft.“
 The field research showed that the joint use of biologics "Lenoil" and “Azole" forThe field research showed that the joint use of biologics "Lenoil" and “Azole" for
bioremediation of oil-contaminated in typical soil make it possible to reduce thebioremediation of oil-contaminated in typical soil make it possible to reduce the
content of petroleum hydrocarbons in the soil for 120 days at 85.7 wt. %.content of petroleum hydrocarbons in the soil for 120 days at 85.7 wt. %.
 Achieved high performance of complex application of biological products "Lenoil",Achieved high performance of complex application of biological products "Lenoil",
“Azole" and "Batsispetsin" to clean typical soil from oil pollution. The degree of“Azole" and "Batsispetsin" to clean typical soil from oil pollution. The degree of
biodegradation of petroleum hydrocarbons for 90 days amounted to 91.5 wt. %.biodegradation of petroleum hydrocarbons for 90 days amounted to 91.5 wt. %.

12. Biodegradation of petroleum products in theBiodegradation of petroleum products in the
recultivated bleaching earth,%recultivated bleaching earth,%

13. ФитотоксичностьФитотоксичность
отбеливающей земли,отбеливающей земли,
всхожесть семян, %всхожесть семян, %
Вариант опытаВариант опыта Laboratory researchLaboratory research Field researchField research
Terms of sampling, daysTerms of sampling, days
CheckCheck 4545±±5,15,1 4141±±1,81,8
bleaching earth+Azolenbleaching earth+Azolen 8686±±6,26,2 5555±±5,25,2
bleaching earth+Lenoilbleaching earth+Lenoil 8888±±8,98,9 6767±±3,33,3
bleachingbleaching
earth+Lenoil+Azolenearth+Lenoil+Azolen
9696±±4,94,9 8585±±1,21,2

14. The level of oil biodegradation in soil contaminatedThe level of oil biodegradation in soil contaminated
with oil (SCO) (% of initial content)with oil (SCO) (% of initial content)
Check
SCO+A
SCO+LSCO+A+L
0
10
20
30
40
50
60
70
80
90
100
30
90
150
4,7
13,8
16,6
10,9
25,7
37,6
27,2
63,7
70,1
27,5
54,9
85,7
%
Days
Л – «Ленойл»
А – «Азолен»
0
10
20
30
40
50
60
70
80
90
100
30
90
150
0
1,5
6,9
6,1
13
22,330
60
70,7
39,2
70,7
76,9
51,5
56,7
66,1
53
75,3
82,3
46,1
60
66,9
39,2
60,7
91,5
%
Days
L – «Lenoil»
А – «Azolen»
B – «Batsispetsin»
Field research Laboratory research

15. Substratum Cultivation time, h
t,
º С
Percent of utilization,
%
Olive oil 24 35 100
Sunflower oil 24 35 79
Cooking oil 48 35 81
Pork fat 48 35 64,2
Mutton fat 120 35 32,6
Olive oil 24 40 90,7
Sunflower oil 24 40 94,5
Cooking oil 48 40 87,5
Pork fat 24 40 70
Mutton fat 48 40 58,4
Utilization of fat by biologicalUtilization of fat by biological
preparation "Lenoil"preparation "Lenoil"
Microorganisms of "Lenoil" biological product are producers of lipolytic
enzymes. The biological product can be used for the local wastewater
treatment of the food industry from fats, oils, removing fat deposits in the
sewer systems of enterprises and fat utilization in grease traps.