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Water relations
Presented by: Syeda Saba Kazmi Dated: 3/9/2015
OUTLINE
Water
• Hydrosphere
•Water structure
•Phases
•Properties
•Water
movement
cont ….
• Cell water related
terminologies
• SPAC
• Water potential
• Tonicity
• Aquaporins
cont ….
• Osmoregulation
• Hofler diagram
• Hydroulic
conductivity
• Psychrometry
• References
Earth: The Biosphere
Hydrosphere Lithosphere Atmosphere
%age of Water on Earth
3/4th of earth surface is
covered by Water
Phases of Water
Gaseous phase: vapor
and clouds in the sky
Liquid Phase: seawater
and rain etc.
Solid Phase: icebergs in
the polar oceans,
glaciers
Water in Cell (Cytoplasm)
•fluid like material
between the cell
membrane and the
nucleus (Cytoplasm)
•over 80 % water
Water in Plants
Water constitutes
80-90% of the fresh
weight of
herbaceous plants,
and more than 50%
of woody species.
Structure of Water
Chemical formula H2O:
one molecule of water
has two hydrogen atoms
covalently bonded to a
single oxygen atom.
Physical & Chemical properties of water
Taste, Odour & color Transmission of Light
Chemical and Physical properties of water
 Surface Tension:
 Boiling Point: 100
degrees Celsius
 Density of Water:
Liquid Phase more
dense
The Universal Solvent Polarity of Water:
Capillary action:
The tendency of water to
move up a narrow tube against
the force of gravity.
E.g. vascular plants, such as
trees.
hydrogen bonding
Each water molecule is
connected to another
water molecule through
weak bonding known as
hydrogen bonding
The Soil Plant
Atmosphere
Continuum(SPAC)
The Soil-Plant-
Atmosphere
Continuum (SPAC) is
the pathway for water
moving from soil
through plants to the
atmosphere.
Cell Water related terminologies
Cohesion: water
molecules stick together, a
process known as
cohesion.
Adhesion: When water
molecules form hydrogen
bonds with other
molecules.
Cell Water related terminologies ( cont …)
Cohesion
•The attraction
water to itself –
H bonds
Cell Water related terminologies ( cont …)
Adhesion
The attraction of water
to other
surfaces/substances
Cell Water related terminologies ( cont …)
Osmosis: Movement of
water from higher
concentration to lower
concentration through
membrane.
Active transport: Reverse
of osmosis, energy is
utilized
Hydrophilic- attracts water & Hydrophobic- repels water
Cell Water related terminologies ( cont …)
Evapotranspiration
•Transpiration is the
water vapor given off
plants
•Evaporation is the
water vapor lost from
the soil
•Evapotranspiration is
the sum of both (Et)
Water pathway through the leaf
water potential.
•Water potential is the property of water that determines
which way it will flow, which depends on the concentration
of solutes in the water.
•Greek letter psi, which looks like this: Ψ.
Components of Water Potential
•Pressure potential (ψp).
-Difference between pressure of the water in cell
and that of the atmosphere= turgor pressure
•Osmotic pressure (solute potential) (ψs).
-Affect of concentrations of solutes inside vs outside
the cell that influence osmosis
•Vapor potential
•Gravitational potential
Pressure Potential
•Turgid cell (left) have a high
positive pressure potential.
•Cells that are flaccid or
plasmolysed (right) have a
pressure potential of zero.
Tonicity
Water Movement Between Plant Cells
• The path through the
plasmodesmata is called the
symplast
• Apoplast is a path through cell
walls and intracellular spaces.
Absorption of water
• Active absorption
• It is the absorption of water by
roots with the help of metabolic
energy generated by the root
respiration.(types)
• Active osmotic water
absorption
• Active non osmotic water
absorption
• Passive absorption
• This mechanism is carried out
without utilization of metabolic
energy
Aquaporin
• Aquaporins are integral
membrane proteins from a
larger family of major intrinsic
proteins (MIP) that form pores
in the membrane of biological
cells.
Discovery
•2003 Nobel Prize in Chemistry
was awarded jointly to Peter
Agre for the discovery of
aquaporin
• Roderick MacKinnon for his
work on the structure and
mechanism of potassium
channels
Function
• Aquaporins are "the plumbing
system for cells," said Agre.
• Also known as water channels
• Aquaporins selectively conduct
water molecules in and out of
the cell
Beta-Barrel
Alpha Helix
Amphipathic Helix
Fatty acid
Chain
GPI-Anchored
Peripheral
Types of Membrane Proteins
Structure
Aquaporin proteins are made up of six transmembrane α-
helices arranged in a right-handed bundle, with the amino and
the carboxyl terminals.
Aquaporins In plants
The presence of aquaporins in the cell membranes seems to serve
to facilitate the transcellular symplastic pathway for water transport
Types(in plants)
Aquaporins in plants are separated into five main homologous
subfamilies, or groups:
1: Plasma membrane Intrinsic Protein (PIP)
2: Tonoplast Intrinsic Protein (TIP)
3: Nodulin-26 like Intrinsic Protein (NIP)
4: Small basic Intrinsic Protein (SIP)
5: X Intrinsic Protein (XIP)
Hofler Diagram: Analysis of change in
torgor and osmotic potential
• Small changes in cell volume give large changes
in ψp
• Hofler slop is actually the study of elastic
modulus(rigidity) of cell wall
Pressure volume curve
Osmoregulation
Osmoregulation is the active regulation of the
osmotic pressure of an organism fluids
Regulators and conformers
Osmoregulation in plants
While there are no specific osmoregulatory organs in higher
plants, the stomata are important in regulating water loss
through evapotranspiration.
• Xerophytes
• Hydrophytes
• Mesophytes
Hydraulic conductivity
Hydraulic conductivity is a property of vascular plants, soils and
rocks, that describes the ease with which a fluid (usually water)
can move through pore spaces or fractures.
Methods of determination
There are two broad categories of determining hydraulic
conductivity:
• Empirical approach by which the hydraulic conductivity is
correlated to soil properties like pore size and particle size
(grain size) distributions and soil texture.
• Experimental approach by which the hydraulic conductivity is
determined from hydraulic experiments.
Psychrometry
Psychrometry is the science of study of various
properties of air, method of controlling its temperature
and moisture content or humidity and its effect on various
materials and human beings.
Composition of Air
Psychrometric Properties of Air
Psychrometry is the science of air.
Study of psychrometric properties of air like:
• Temperature,
• Relative humidity
• dew point temperature
• Composition of air
References
• http://www.tutorvista.com/content/biology/biology-iv/plant-water-relations/absorption-water-by-
plants.php
• http://www.springerlink.com/content/qw62632354v4nm77/
• http://www.ictinternational.com.au/appnotes/ICT101.htm
• http://www.jstor.org/stable/4353618
• http://www.wiziq.com/tutorial/70692-Biology-XI-11-Transport-in-plants-4-Mechanism-of-Water-Absorption
• http://www.uoguelph.ca/~mgoss/four/410_N05.html
• www.slideworld.com/.../Water-relation-Soil-Plant-Atmosphere-continu
• https://frank.itlab.us/learning/xylem.ppt
• www.prairiesoilsandcrops.ca/articles/volume-6-11-screen.pdf
• https://pilgrimapbio.files.wordpress.com/2011/11/water-potential.ppt
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water relations

  • 1.
  • 2.
  • 3. Water relations Presented by: Syeda Saba Kazmi Dated: 3/9/2015
  • 4. OUTLINE Water • Hydrosphere •Water structure •Phases •Properties •Water movement cont …. • Cell water related terminologies • SPAC • Water potential • Tonicity • Aquaporins cont …. • Osmoregulation • Hofler diagram • Hydroulic conductivity • Psychrometry • References
  • 5. Earth: The Biosphere Hydrosphere Lithosphere Atmosphere
  • 6. %age of Water on Earth 3/4th of earth surface is covered by Water
  • 7.
  • 8. Phases of Water Gaseous phase: vapor and clouds in the sky Liquid Phase: seawater and rain etc. Solid Phase: icebergs in the polar oceans, glaciers
  • 9. Water in Cell (Cytoplasm) •fluid like material between the cell membrane and the nucleus (Cytoplasm) •over 80 % water
  • 10. Water in Plants Water constitutes 80-90% of the fresh weight of herbaceous plants, and more than 50% of woody species.
  • 11. Structure of Water Chemical formula H2O: one molecule of water has two hydrogen atoms covalently bonded to a single oxygen atom.
  • 12. Physical & Chemical properties of water Taste, Odour & color Transmission of Light
  • 13. Chemical and Physical properties of water  Surface Tension:  Boiling Point: 100 degrees Celsius  Density of Water: Liquid Phase more dense
  • 14. The Universal Solvent Polarity of Water:
  • 15. Capillary action: The tendency of water to move up a narrow tube against the force of gravity. E.g. vascular plants, such as trees.
  • 16. hydrogen bonding Each water molecule is connected to another water molecule through weak bonding known as hydrogen bonding
  • 17. The Soil Plant Atmosphere Continuum(SPAC) The Soil-Plant- Atmosphere Continuum (SPAC) is the pathway for water moving from soil through plants to the atmosphere.
  • 18. Cell Water related terminologies Cohesion: water molecules stick together, a process known as cohesion. Adhesion: When water molecules form hydrogen bonds with other molecules.
  • 19. Cell Water related terminologies ( cont …) Cohesion •The attraction water to itself – H bonds
  • 20. Cell Water related terminologies ( cont …) Adhesion The attraction of water to other surfaces/substances
  • 21. Cell Water related terminologies ( cont …) Osmosis: Movement of water from higher concentration to lower concentration through membrane. Active transport: Reverse of osmosis, energy is utilized
  • 22. Hydrophilic- attracts water & Hydrophobic- repels water
  • 23. Cell Water related terminologies ( cont …) Evapotranspiration •Transpiration is the water vapor given off plants •Evaporation is the water vapor lost from the soil •Evapotranspiration is the sum of both (Et)
  • 25. water potential. •Water potential is the property of water that determines which way it will flow, which depends on the concentration of solutes in the water. •Greek letter psi, which looks like this: Ψ.
  • 26. Components of Water Potential •Pressure potential (ψp). -Difference between pressure of the water in cell and that of the atmosphere= turgor pressure •Osmotic pressure (solute potential) (ψs). -Affect of concentrations of solutes inside vs outside the cell that influence osmosis •Vapor potential •Gravitational potential
  • 27. Pressure Potential •Turgid cell (left) have a high positive pressure potential. •Cells that are flaccid or plasmolysed (right) have a pressure potential of zero.
  • 28.
  • 30. Water Movement Between Plant Cells • The path through the plasmodesmata is called the symplast • Apoplast is a path through cell walls and intracellular spaces.
  • 31. Absorption of water • Active absorption • It is the absorption of water by roots with the help of metabolic energy generated by the root respiration.(types) • Active osmotic water absorption • Active non osmotic water absorption • Passive absorption • This mechanism is carried out without utilization of metabolic energy
  • 32. Aquaporin • Aquaporins are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells.
  • 33. Discovery •2003 Nobel Prize in Chemistry was awarded jointly to Peter Agre for the discovery of aquaporin • Roderick MacKinnon for his work on the structure and mechanism of potassium channels
  • 34. Function • Aquaporins are "the plumbing system for cells," said Agre. • Also known as water channels • Aquaporins selectively conduct water molecules in and out of the cell
  • 35. Beta-Barrel Alpha Helix Amphipathic Helix Fatty acid Chain GPI-Anchored Peripheral Types of Membrane Proteins
  • 36. Structure Aquaporin proteins are made up of six transmembrane α- helices arranged in a right-handed bundle, with the amino and the carboxyl terminals.
  • 37. Aquaporins In plants The presence of aquaporins in the cell membranes seems to serve to facilitate the transcellular symplastic pathway for water transport
  • 38. Types(in plants) Aquaporins in plants are separated into five main homologous subfamilies, or groups: 1: Plasma membrane Intrinsic Protein (PIP) 2: Tonoplast Intrinsic Protein (TIP) 3: Nodulin-26 like Intrinsic Protein (NIP) 4: Small basic Intrinsic Protein (SIP) 5: X Intrinsic Protein (XIP)
  • 39. Hofler Diagram: Analysis of change in torgor and osmotic potential • Small changes in cell volume give large changes in ψp • Hofler slop is actually the study of elastic modulus(rigidity) of cell wall
  • 40.
  • 41.
  • 43. Osmoregulation Osmoregulation is the active regulation of the osmotic pressure of an organism fluids Regulators and conformers
  • 44. Osmoregulation in plants While there are no specific osmoregulatory organs in higher plants, the stomata are important in regulating water loss through evapotranspiration. • Xerophytes • Hydrophytes • Mesophytes
  • 45. Hydraulic conductivity Hydraulic conductivity is a property of vascular plants, soils and rocks, that describes the ease with which a fluid (usually water) can move through pore spaces or fractures.
  • 46.
  • 47. Methods of determination There are two broad categories of determining hydraulic conductivity: • Empirical approach by which the hydraulic conductivity is correlated to soil properties like pore size and particle size (grain size) distributions and soil texture. • Experimental approach by which the hydraulic conductivity is determined from hydraulic experiments.
  • 48. Psychrometry Psychrometry is the science of study of various properties of air, method of controlling its temperature and moisture content or humidity and its effect on various materials and human beings.
  • 50. Psychrometric Properties of Air Psychrometry is the science of air. Study of psychrometric properties of air like: • Temperature, • Relative humidity • dew point temperature • Composition of air
  • 51. References • http://www.tutorvista.com/content/biology/biology-iv/plant-water-relations/absorption-water-by- plants.php • http://www.springerlink.com/content/qw62632354v4nm77/ • http://www.ictinternational.com.au/appnotes/ICT101.htm • http://www.jstor.org/stable/4353618 • http://www.wiziq.com/tutorial/70692-Biology-XI-11-Transport-in-plants-4-Mechanism-of-Water-Absorption • http://www.uoguelph.ca/~mgoss/four/410_N05.html • www.slideworld.com/.../Water-relation-Soil-Plant-Atmosphere-continu • https://frank.itlab.us/learning/xylem.ppt • www.prairiesoilsandcrops.ca/articles/volume-6-11-screen.pdf • https://pilgrimapbio.files.wordpress.com/2011/11/water-potential.ppt