Leukocytes

1. White Cell Precursors
(Myeloid)
Prepared By : A . Moghaddam
Hematology Supervisor at
Pars Hospital Lab

3. Granulocytic Maturation Diagram
340 h (14 day)

5. Myeloblast

6. The least mature precursor in granulopoiesis
is the myeloblast, which is released into the blood stream
only under pathological conditions. A large myeloblast is
shown with a fine reticular nuclear structure and
a narrow layer of slightly basophilic cytoplasm without
granules (Honey Pattern).

7. Myeloblast and neutrophilic granulocytes with
segmented nuclei (blood smear from a patient
with AML).

8. Auer Rods

9. Promyelocyte

10. Myeloblast (1), which
shows the start of
azurophilic granulation
(arrow),
Promyelocyte (2)
with copious large
azurophilic granules,
typically in a perinuclear
location.

12. Myelocyte

13. • Myelocyte , (Early myelocyte)
The chromatin structure is denser than that of promyelocytes.
The granules do not lie over the nucleus (as can be seen by
turning the fine focus adjustment of the microscope to and
fro). The blood smear is from a case of sepsis, hence the
intensive granulation.

14. Metamyelocyte

15. 1) This Metamyelocyte is distinguished from a myelocyte by
incipient lobe formation.
2) Transitional form between a metamyelocyte and a
band cell.
2
1

16. Band (Stab)

18. 1) Band cells can also occur as aggregates.
2) Segmented neutrophilic granulocytes.
1 2

19. Segmented
Neutrophil

21. 1) Large Promyelocyte
2) myelocyte
3) metamyelocyte
4) polychromatic erythroblast
(4)

22. 1- Typical myelocyte
2- segmented Neutrophil.

23. • Hyper segmented neutrophilic granulocyte (six
or more segments).
There is an accumulation of these cells in Megaloblastic anemia.
• Drumstick (arrow 1) as an appendage with a thin filament
bridge to the nucleus (associated with the X-chromosome),
adjoined by a thrombocyte (arrow 2).

27. Reactive state with Toxic granulation of the
neutrophilic granulocytes, more visibly expressed in the
cell on the left (1) than the cell on the right (2) (compare
with non activated cells.

28. 1) Copious granulation in a band cell (1) (toxic
granulation) next to band cells (2) with Döhle bodies
(arrows).
2) band cells.
2

30. • Reactive state with Toxic granulation of the
neutrophilic granulocytes, more visibly expressed in the cell
on the left (1) than the cell on the right (2) (compare with non
activated cells.
• Sepsis with toxic granulation, cytoplasmic vacuoles, and
Döhle bodies (arrows) in band cells (1) and a monocyte (2).

31. Eosinophil

32. Basophil

33. LYMPHOCYTES
&
MONOCYTES

35. •Lymphoblast.

36. •Lymphoblast.

43. •Prolymphocytes.

44. •Prolymphocytes.

45. •lymphocytes.

46. • Hairy Cell Leukemia

47. Reactive lymphocyte

49. • Reactive large lymphocyte with moderately
abundant gray-blue cytoplasm (1000×).

50. • Smudge cell. Nuclear remnant
from a damaged/broken white cell

51. • Broken cell. A broken cell of the myeloid
series with ruptured cell membrane an
disintegration of cytoplasmic contents
(1000×).

52. • Ruptured cell.
Ruptured/disintegrating leukocyte
(1000×).

53. •Basket cells (1000×).

54. • Lymphocyte. This is a benign reactive lymphocyte
with moderately abundant pale gray cytoplasm
hugging the surrounding red cells and distinct
separation of chromatin/parachromatin (1000×).

55. • Large granular lymphocyte with
azurophilic cytoplasmic granules
(Wright– Giemsa, 1000×)

56. • Plasma cell. Eccentric round nucleus
with clumped nuclear chromatin and
moderate amount of basophilic cytoplasm
with prominent nuclear, or clear zone
(1000×).

57. • Plasma cells with abundant basophilic
cytoplasm, eccentric nucleus, and perinuclear
clear Golgi zone (Wright– Giemsa, 1000×).

61. Monocyte

67. Thanks