Which of the following is not a premise of the Koch’s postulates? A. The same microbe must be present in all cases of the disease in question. B. The microbe must be isolated from the infected organisms and grown in a pure culture. C. A test animal inoculated with the microbe from a pure culture should develop the disease. D. Several microbe species must be isolated in a pure culture from the samples of the test animal. Solution D. Several microbe species must be isolated in a pure culture from the samples of the test animal. Explanation: According to Koch the microorganism must be reisolated from the inoculated, diseased experimental host and identified as being identical to the original specific causative agent..