VITAMIN B12 production

1. Submitted to:
Dr.S.Viswanathan,
Head of the department,
Department of Microbiology,
Sri paramakalyani college.
Alwakurichi-627412
INDUSTRIAL MICROBIOLOGY
SEMINAR PRESENTATION
IV- SEMESTER
Topic –VITAMIN B12

3. Content
• Introduction
• Source and requirement
• Microbial production
• Steps involve in production process
• Fermentation of process vitamin
• Media preparation
• Purification
• Application

4. Introduction
 Vitamin B12,also known as cobalamin.
 It was first isolated from liver cells of animals in 1942
by Rickes and Smith.
 It is a water soluble vitamin involved in the
metabolism of cells of the human body.
 It is one of eight B vitamins.
 It is a cofactor in DNA synthesis,and both fatty acid
and amino acid metabolism.
 It is important in Normal functioning of the brine and
nervous system as well as in the formation of the
blood

5. Requirements
The Reference Daily Intake (RDI) is about 2.4 mcg and little
higher consumption is required for pregnant woman as well
as breast feeder.
Deficiency may cause Pernicious anemia (not enough healthy
red blood cells).
Strict vegetarians are at the risk of its deficiency, as the food
of non-animal origins is free from cobalamine unless
contaminated by bacteria.

6.  It was first isolated from liver cells of animals in 1942 by Rickes and
Smith. These vitamins are entirely manufactured through the biosynthetic
fermentation process with the help of handpicked and genetically optimized
micro-organisms.
 Almost all microbes are capable of synthesizing Vitamins and other
growth regulating substances required for their cells. But, only a few of
them produce vitamins in larger proportions.
 Due to these reasons,Propionibacterium shermanii and Pseudomonas
denitrificans strains are used for the industrial production of Vitamin
B12. However, P. denitrificans are widely used and, it is believed that we can
achieve 300 mg/l of efficiency while using P.denitrificans.
Microbial production

7. •Some of the bacteria associating with the production of
vitamin B12 are
• Aerobacter,
• Agrobacterium,
• Alcaligenes,
• Azotobacter,
• Bacillus
• Clostridium,
• Salmonella,
• Corynebacterium,
• Flavobacterium,
• Micromonospora,
• Mycobacterium,
• Nocardia,
• Propionibacterium,
• Protaminobacter,
• Proteus,

25. APPLICATION
Cyanocobalamine has the following uses;
• It’s a food preservative
• It’s a co- factor
• It’s a protective medicine