This document discusses urban science and sustainability research at the USDA. It outlines several areas of focus for the USDA including forest inventory and management, ecosystem services, human health and well-being, urban sustainability, green infrastructure, water and watersheds, and urban long-term research. It provides examples of specific projects studying urban stewardship networks in Baltimore and tools created by the USDA Urban Agriculture Working Group to support urban agriculture through resources on business planning, land access, soil quality, water access, and more. In closing, it notes the USDA supports best practices through urban research on topics like nutrition interventions, crop production methods like vertical farming, and social science.