Tuesday theme 1 1415 1430 large briefing room hannele

1. APA, Addis, October, 2016
Trait Observation and Discovery Network for Potato
Hannele Lindqvist-Kreuze, Greg Forbes,
Kaiyun Xie, Awais Khan, Britta Kowalski
and Merideth Bonierbale

2. Background problem
● Potato yields of 10-15t/ha in CIP target
regions are far lower than the attainable
yield
● Process of breeding and release of potato
varieties is slow, can take 12-15 years
● Faster genetic gains are required to
improve the yields

3. Project purpose
Faster
genetic
gains
New
trait
sources
New
methods
and tools
Trait
data
• Panel of diverse elite
potato lines distributed to
NARS
• Capacity building of
NARS researchers
• Trait evaluation
protocols
• Data analysis and data
management tools
• Large scale on site selection of
candidate varieties and trait sources
• Decentralized population improvement
through systematic data collection
• User preferences
• Observations of other risks and
constraints of local importance
• Baseline trait levels
with standard
protocols
• Genotyping
• GWAS in multiple
target environments

4. Project partners
Click to edit Master subtitle style
CIP
Max Planck
EIAR
ARARI
YAAS
HAAS
GAU
Funding:
• BMZ (2015-2017)
• RTB
• Partner
complementary
projects

5. Diverse panel of elite potato clones (367)
B3: Resistance to late blight;
adaptation to highland tropics
tuberosum
andigena
demissum
phureja
neo tuberosum
LTVR: Resistance to PVY, PVX,
PLRV; Heat tolerance, adaptation to
lowland tropics
LTVR – B3:
Late blight
and virus
resistance
Highland tropics late blight
resistant populations
A and B1
B3-HT:
Late blight resistant, heat
tolerant

6. Country/Region Agro-ecologies Maturity Drought Virus
Late
blight
Ethiopia
Adet
Mid, highland
x x
Holetta x x x
China
Yunnan
lowland &
highland
x x x x
Heilongjiang
Temperate, long
day
x x
Gansu Dry land x x
Peru
La Molina Lowland sub-
tropics
x x x
Ica x
Oxapampa
Highland
tropics
x
Trait Evaluations

7. Standardized protocols…examples
Lateblightresistance
• Symptom
descriptions
• Experimental
design suggestions
• Trait evaluation
protocol
• Recoding and
analyzing data
• Field book template
Tuberbulking
• Trait description:
tuber growth,
physiology
• Experimental
design
• Evaluation
parameters
• Data analysis
• Field book template

8. Yunnan late blight resistance trial
Histogram distribution of the sAUDPC
values of CIPs advanced potato lines in
Yunnan, China, July 2015.
0
10
20
0 5 10
SAUDPC
count
Yunnan SAUDPC histogram, Desiree=
C88 Desiree

9. Yunnan late blight trial
Boxplot of the sAUDPC values of CIPs
advanced lines in the different breeding
populations.
0.0
2.5
5.0
7.5
10.0
12.5
A B1 B3 B3-HT B3-LTVR BW LTVR LTVR-B3 PREBREDVARIETY
factor(population_group)
SAUDPC
Yunnan SAUDPC/breeding population
C88

10. SNP markers Chr SolCAP 8K array GBS
0 243 5,474
1 299 11,880
2 343 6,648
3 228 10,157
4 297 10,391
5 211 8,828
6 253 8,089
7 294 8,569
8 219 8,678
9 246 9,429
10 163 7,608
11 204 6,216
12 192 9,714
3,192 111,681
SNP
screened 8,300 330,448

11. Population sub-structuring

12. Marker-trait associations after Bonferroni correction
Yunnan 2015 Peru 2014 Peru 2012

13. Variety preference and gender
• Important to understand quality traits preferred by
users to allow for more gender responsive targeting
• Building on the RTB work on integrating gender into
Participatory Varietal Selection (PVS) for potato in
Ethiopia and RTB Database of user preferences
• 2016 test for stakeholder expressed preference
using new potato genotypes

14. PVS trial in Adet 2016
• 15 clones from the diversity panel planted in the field in Adet in
July 18
• First evaluation done at the flowering stage on September 23:
Participants: Farmers=34 (12 Female +22 Male)
Women ranking the
best crop parameters
Crop parameters
for variety
selection by the
women group
Farmers ranking the clones based
on the crop parameters

15. Summary
Base
populations
Standardized
protocols
Data analysis
tools
Trait
evaluationsGenotyping
Trait-
marker
association
PVS

16. Research team:
CIP-Lima:
Merideth Bonierbale
Hannele Lindqvist-Kreuze
Awais Khan
CIP-Ethiopia:
Britta Kowalski
Hirut Getinet
Mikias Yeshitila
CIP-China:
Greg Forbes
Kaiyun Xie
Junhong Qin
Germany:
Karin Koehl Max Planck
Ethiopia:
Gebremedhin Woldegiorgis EIAR
Alemu Worku ARARI
Tesfaye Desta ARARI
China:
Xianping Li YAAS
Junlian Zhang GAU
Wangmin Sheng HAAS

17. Thanks for your attention