The document discusses several fluorescence-based assay readout formats for measuring ATP conversion that are validated for multimode readers and provide good signal stability over 8 hours. It recommends using the enzyme at 60-85% of its saturating concentration to accurately determine IC50 values directly from raw fluorescence data without needing a standard curve, and provides equations to calculate optimal antibody concentration based on ATP concentration between 0.1 to 1000 μM.