The Veterinary Health Management Of The Life Cycle1
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This document outlines the management of the life cycle stages of the Japanese medaka fish (Oryzias latipes) in order to develop a bioassay system for testing waterborne carcinogens. It describes maintaining a breeding colony of 20 medaka tanks and protocols for the different life stages - eggs, embryos, larvae, fry, and adults. Water quality is carefully managed by monitoring chemistry, temperature, and microbial levels. The conclusion states that under specific chemical-free conditions controlling water chemistry, photo-period, and nutrition, a medaka breeding colony can be maintained for optimal growth.
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Organochlorine pesticides were measured across the food web of Lagos Lagoon, Nigeria. Sediment had the highest total concentrations of organochlorine pesticides, ranging from 0.00 to 1673.57 μg/kg. Certain species, such as the shrimp Melicertus keraturus, accumulated high levels of pesticides, posing health risks to humans. Pesticide concentrations in biota were influenced by habitat, feeding habits, and lipid composition. Continued monitoring is needed to assess and address pesticide pollution in the lagoon.
Annual environment and health conference 2018 speaker4 thuy do_stoppingantibi...
Annual environment and health conference 2018 speaker4 thuy do_stoppingantibi...Environmental Protection Agency, Ireland
This document summarizes a study on antibiotic resistance in wastewater treatment plant (WWTP) effluent. The study analyzed microbial indicators, antibiotic resistance genes, bacterial communities, potential pathogens, and plasmids in effluent from several WWTPs. Results showed the presence of antibiotic resistant bacteria, resistance genes, and multidrug resistant plasmids in WWTP effluent, which poses a risk to the environment and human health. The work was funded by the EPA and Irish Water and conducted by researchers at the Antimicrobial Resistance and Microbiome Project laboratory.Microbial and chemical analysis of potable water
This document presents the results of a study analyzing the microbial and chemical composition of potable water sources within Lagos University in Nigeria. Water samples were collected from four tap sources on campus and tested for various microbes using membrane filtration and agar plating. Tests found generally acceptable chemical quality according to WHO standards, with the mean numbers of microbial species and selected chemical properties reported for the water distribution system. The study aimed to evaluate water safety and suggest methods for reducing water-borne disease incidence on campus.
Genotoxicity
The document provides information about genotoxicity testing. It discusses:
1) The origins and development of genetic toxicology as a field of science and the major guidelines for testing like OECD, ICH, and Schedule Y.
2) Standard tests for genotoxicity including the Ames test, micronucleus assay, and comet assay.
3) Mechanisms of action by which genotoxic substances can damage DNA and mechanisms like point mutations and chromosomal mutations.
4) The importance of genotoxicity studies in identifying substances that can damage genetic material.
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Selection of Biological Methods for Industrial Effluents Assessments
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The Veterinary Health Management Of The Life Cycle1
- 1. The Veterinary HealthThe Veterinary Health Management Of The LifeManagement Of The Life Cycle Stages of TheCycle Stages of The Japanese medaka (Japanese medaka ( OryziasOryzias latipeslatipes)) Horace E. Walcott, DVM, MSPH, MScHorace E. Walcott, DVM, MSPH, MSc Scientific Fellow Zoological Society ofScientific Fellow Zoological Society of LondonLondon Brooklyn TechBrooklyn Tech
- 2. The BlackThe Black BoxBox InIn The LMMThe LMM
- 3. Carcinogen Concentration (ppt) Log Of RISK ? ?????? ??????
- 4. ObjectivesObjectives • Develop a bioassay system for waterDevelop a bioassay system for water borne carcinogensborne carcinogens • Toxicological Pathology StudiesToxicological Pathology Studies • Maintain a breeding colony of 20 tanks ofMaintain a breeding colony of 20 tanks of medakamedaka
- 5. SCcFESCcFE
- 6. Chemicals?Chemicals? • Methylene BlueMethylene Blue • Sea SaltSea Salt • MS222 (Tetracaine)MS222 (Tetracaine)
- 7. Concentrations/DosagesConcentrations/Dosages • MB 1 ml Stock Solution:1LMB 1 ml Stock Solution:1L of Waterof Water • MS222 50 – 130 mg/L ofMS222 50 – 130 mg/L of Water & 1 g/L of waterWater & 1 g/L of water
- 10. Life Cycle StagesLife Cycle Stages • EggEgg • EmbryoEmbryo • LarvaLarva • FryFry • AdultAdult
- 11. Aquatic Medicine & BiologyAquatic Medicine & Biology ProtocolProtocol Egg & Embryo Breeding Adults Larva & Frey Juveniles
- 12. Water Quality ManagementWater Quality Management Chemistry Profile Temperature Microbial Culture
- 13. ConditionsConditions • pH = 6.8pH = 6.8 ±± 1.21.2 • Tank Temperature = 21.6Tank Temperature = 21.6 ±± 6.06.0 °°CC
- 14. ConclusionConclusion A breeding colony of medakaA breeding colony of medaka can be maintained undercan be maintained under specific chemical freespecific chemical free (carcinogen) conditions, in(carcinogen) conditions, in which water chemistrywhich water chemistry parameters, photo-periodparameters, photo-period and nutrition contribute toand nutrition contribute to optimal growth.optimal growth.
- 15. AcknowledgementsAcknowledgements Dr. William R. HartleyDr. William R. Hartley Dr. William A. Toscano, JrDr. William A. Toscano, Jr Dr. Asaf AbdelghaniDr. Asaf Abdelghani Dr. Mary B. AndersonDr. Mary B. Anderson Ms. Jay BeeMs. Jay Bee Dr. Chad RoyDr. Chad Roy Dr. Chad NelsonDr. Chad Nelson
- 16. Best Aquatic Rat?Best Aquatic Rat? Oryzias latipes