2. WHAT IS FILTRATION?
• Filtration separates particles based on size difference through the pores of a
membrane known as a filter.
• The fluid or particle that is smaller than the size of the pores in the filter
passes through a filter (filtrate or permeate) while the bigger particles will
be trapped by the filter (retentate).
3. TYPES OF FILTRATION
Filtration can be performed in one of two operational modes
• Normal Flow Filtration
• Tangential Flow Filtration
4. NORMAL FLOW FILTRATION
• Dead End Filtration
• Direct Flow Filtration (DFF)
• Feed flows in perpendicular to the
to the filter membrane.
7. WHAT DOES A TFF DO?
• Purification (separates components by size, removes impurities)
• Concentration (reduces volume, de-waters)
• Buffer Exchange or diafiltration (formulation)
9. APPLICATIONS OF TANGENTIAL FLOW FILTRATION
• Separation of large and small biomolecules, clarification and/or removal of
contaminants.
• Purify and/or recover plasmid DNA from cell culture or cDNA from blood.
• Cell harvesting (bacteria, yeast, mammalian cells.)
• Reduce Bioburden and/or clean up water, solutions, buffers, and media
(remove and/or reduce endotoxin load.)
• Recover and or remove virus particles and/or vaccines.