Swamy ibp
- 1. Breeding for ImprovingDrought Tolerance in Rice Mega Varieties: Progress at IRRI Integrated plant breeding Platform annual meeting June 1-3, 2011, Wageningen Netherlands B.P. Mallikarjuna Swamy
- 2. “I challenge thenext generation to use new scientific tools and techniques to address the problems that plague the world’s poor.” Dr. Norman Borlaug hastens the process of development of Commercial Products
- 3. Drought at reproductivestage is more devastating, causes severe reduction in the yield 7000 Donor Yield Yield 6000 Yield of cultivar (kg ha-1) Stress Non- stress 5000 kg ha-1 4000 MTU1001 312 5825 3000 Madhuri line 9 312 4957 2000 Nidhi 356 4963 1000 IR64 278 4685 0 Mahamaya 318 4344 Severe Moderate Control Swarna 312 5822 stress level Mahamaya Sambha Mahsuri
- 4. Major goal Development ofdrought tolerant rice varieties with an yield advantage of 1.0 to 1.2 tha over the control • High yield under normal situation • Tolerant to blast, brown spot, bacterial leaf blight • Good grain quality/quality maintenance under drought • High framers’ preference
- 5. Drought Research atIRRI: Strategy Conventional approaches • Use improved pre-breeding lines as donors • Direct selection for grain yield • Combine high yield potential with good yield under drought • Confirm performance in multi location testing in target environment-Drought breeding network Molecular approaches • Use traditional/wild donors in mapping populations • Identify major drought yield QTLs • Introgress QTLs in improved drought susceptible varieties by MAB • Physiological and molecular mechanism of QTLs drought tolerance
- 6. Some initial success • During 2009-11, 6 varieties: Sahbhagi dhan-IR74371-70-1-1 in India, Tarharra 1 -IR 80411-B-49-1, Sukha dhan 1- IR74371-46-1-1, Sukha dhan 2- IR74371-54-1-1, Sukha dhan 3- IR74371-70-1-1 in Nepal and Sahod Ulan 1 - IR74371-54-1-1 in Philippines released. • Release of IR74371-70-1-1 in Bangladesh under process. • These provide 0.8-1.2 t ha -1 yield advantage under moderate to severe Sahbhagi dhan in India drought while maintaining high yield under normal rainfall situation. Tarharra 1 in Nepal Sahod Ulan 1 in Philippines
- 7. Promising lowland droughttolerant lines at IRRI DESIGNATION PARENTAGE GYNS GYS % IMP IR 84859-B-94-2-3 IR 70181-3-PMI 1--/IR 72667-16-1-B-B-3 6308 2233 93 IR 83894-B-B-46-4 IR 70213-10-CPA 4-2-2-2/IRRI 122 6175 2213 92 IR 84878-B-60-4-1 IR 71700-247-1-1/IRRI 123 6137 2419 109 IR 84850-B-27-1-1 IR 70181-32-PMI 1-/IR 71700-247-1-1 6091 2364 105 IR 84850-B-27-1-2 IR 70181-32-PMI 1-/IR 71700-247-1-1 6075 2299 99 IR 83383-B-B-129-4 IR 72022-46-2-/IR 57514-PMI 5 5761 2167 88 IR 83383-B-B-141-1 IR 72022-46-2-/IR 57514-PMI 5 5689 2417 109 SWARNA VASISTHA/MAHSURI 6179 672 -42 SAMBHA MAHSURI SAMBHA MAHSURI 5581 272 -76 PSBRc 80 IR 50401-77-2-1-3/IR 42068-22-3-3-1-3 4669 1155 SED 540 298
- 8. Promising upland droughttolerant lines at IRRI DESIGNATION DTF GYI GYD >Apo% IR 81063-B-94-U 3-2 70 4273 3324 124 IR 74371-46-1-1 71 4586 3050 106 IR 79913-B-20-B-2 71 3807 2895 95 IR 79913-B-33-B-4 74 3721 2881 95 IR 79971-B-93-B-3 61 3712 2839 92 IR 81063-B-94-U 3-1 76 4579 2627 77 IR 81044-B-112-U 4-2 73 3604 2464 66 IR 78875-131-B-1-1 76 3537 2451 65 IR 81429-B-31 67 4061 2325 57 IR 78875-190-B-1-3 76 4323 2247 52 IR 81039-B-173-U 3-3 76 3911 2234 51 APO 87 3888 1481 0 SED 2 354 423
- 9. DT lines inAfrica- Mozambique- On-farm PVS Nicoadal Variety Mucelo 1 Mucelo 2 a Licuar Ceramica Mean IR77080-B-34-3 1583 4072 2876 2502 2379 2682 IR80411-B-49-1 1951 3533 2826 748 2439 2299 IR80416-B-32-3 1460 3579 3778 2447 2131 2679 IR81187-B-B-110-1 1520 3748 3729 2075 2570 2728 IR81192-B-B-124-1 1199 3060 3338 2096 2434 2425 IR81313-154-1-2-1-3 1829 2948 2026 1496 1904 2041 IR81314-52-3-3-2-3 2253 3351 3102 722 2110 2308 IR81496-17-2-2-3 2255 3575 3605 2693 2481 2922 IR82004-13-3-2 1626 3246 2226 1402 2230 2146 IR82024-40-1-3-B 1066 2692 3461 1357 1773 2070 IR82077-B-B-71-3 1476 3136 2657 1754 1843 2173 IR82288--B-B-95-4 1591 3302 3618 1697 1762 2394 IR82292-B-B-107-3 1663 3341 4114 2011 2833 2793 IR82574-543-3-1 1494 4225 3035 2170 2505 2686 IR82578-640-3-3 2893 3167 2927 1052 2638 2535 IR84129-18-B 1417 3381 2871 2524 2019 2442 ITA312 1733 2866 2906 1035 2091 2126 Nene 815 1598 4121 786 S. Surapong, 1617 767 2010
- 10. On-farm performance, acceptance Genotypes Yield Duration Height Mean Preference (t ha-1) (days) (cm) Score Rank BR7358-40-2-3-2 2.24 98 88 -0.124 2nd (-) IR83614-427-B 3.93 101 87 -0.046 3rd (-) NERICA 4 1.97 107 102 -0.138 1st (-) BR7873-5*(Nils) 3.47 122 110 0.011 IR83376-B-B-130-2 4.06 113 113 0.016 IR83381-B-B-6-2 4.37 113 111 0.047 IR83381-B-B-6-1 4.49 114 108 0.065 3rd (+) IRRI 123 4.92 122 106 0.074 2nd (+) IR83388-B-B-8-3 4.11 118 103 -0.042 IR83376-B-B-150-1 3.88 117 108 -0.037 IR83377-B-B-93-3 4.97 119 108 0.118 1st (+) BR7465-1-2-4 4.27 129 93 -0.002 BRRI dhan49 (ck) 4.62 133 102 0.043 Guti Swarna (ck) 4.77 140 109 0.014 BRRI dhan 33 (ck) 3.97 111 106 0.005 Biswajit, BRRI, Rajshahi, Bangladesh and Stephan, IRRI 2010, STRASA
- 11. Major drought yieldQTLs in background of improved popular varieties Genetic Additive Background QTLs Ecosystem effect % mean Vandna DTY12.1 Upland 47 IR64 DTY1.1 Lowland 32 IR64 DTY9.1 Lowland 27 IR64 DTY10.1 Lowland 22 IR64 DTY2.1 Lowland 13 IR64 DTY4.1 Lowland 14 Swarna DTY1.1 Lowland 25 Swarna DTY2.1 Lowland 19 Swarna DTY3.1 Lowland 25 Swarna DTY8.1 Lowland 16 MTU1010 DTY1.1 Lowland 17
- 12. Improved IR64 introgressionlines Line GS QTLs GY Drought GY Control (%) DS09 DS10 DS09 WS10 IR 87729-69-B-B-B DTY9.1, DTY2.1, DTY10.1, DTY4.1 2006 2011 6936 4627 94.4 IR 87728-102-B-B DTY9.1, DTY10.1,DTY4.1 2440 1160 6059 5462 92.9 IR 87707-186-B-B-B DTY2.1, DTY10.1,DTY4.1 3200 2068 6289 6737 96.9 IR 87707-446-B-B-B DTY2.1, DTY4.1 3624 2556 6005 6076 97.0 IR 87707-445-B-B-B DTY2.1, DTY4.1 3639 2555 8006 5565 96.9 IR 87707-118-B-B-B DTY2.1, DTY4.1 3264 2273 6096 4617 95.8 IR 87705-21-13-B DTY2.1 2223 4785 6231 95.8 IR 87705-6-8-B DTY4.1 2152 5399 5576 95.5 IR 87728-395-B-B DTY9.1 1122 5500 5457 93.4 IR 87705-36-3-B DTY10.1 2062 5052 7211 95.3 IR64 567 636 4151 5811 M. Swamy, IRRI, 2011
- 13. Quality traits ofIR64 introgression lines LINES QTLs DTF PH AC GT MP CS IR 87729-69-B-B-B DTY9.1, DTY2.1, DTY10.1, DTY4.1 86 98 20.7 I 1 1 IR 87728-102-B-B DTY9.1, DTY10.1,DTY4.1 86 101 20.1 I 1 1 IR 87707-186-B-B-B DTY2.1, DTY10.1,DTY4.1 82 107 21.6 I 2 1 IR 87707-446-B-B-B DTY2.1, DTY4.1 81 106 22.2 I 1 1 IR 87707-445-B-B-B DTY2.1, DTY4.1 83 111 22.3 I 1 1 IR 87707-118-B-B-B DTY2.1, DTY4.1 83 108 20.7 I 1 1 IR 87705-21-13-B DTY2.1 82 86 21 I 2 1 IR 87705-6-8-B DTY4.1 80 85 21 I/L 2 1 IR 87728-395-B-B DTY9.1 86 100 20.2 I 1 2 IR 87705-36-3-B DTY10.1 87 84 20.3 I 1 1 IR64 82 105 21.8 I/L 1 1 M. Swamy, IRRI
- 14. IR 64 introgressionlines with DTY QTLs + QTL - QTL IR64 IR64+DTY QTLs Parents- 2007 Introgressions under drought- 2010 DTY IR 64 introgressed line Similar to IR64 grain quality traits of Product - 2011 introgressed lines
- 15. Line with DTY QTLs IR64
- 16. DTY3.1, DTY2.1 inSwarna, Swarna sub1 Swarna lLs (DTY +Sub1) Swarna M. Swamy, IRRI
- 17. DTY1.1, DTY3.1, DTY2.1in Swarna, Swarna sub1 BC4F1 Swarna lines (three QTLs +Sub1) *2 in 1 rice for drought and submergence prone areas
- 18. Development of improvedVandana with DTY12.1 Grain yield (Kgha-1) % Lines Generation DTF PHT USS UMS UNS BG A IR 84984-83-15-110-B BC2F2:4 299 1514 4855 54 124 92.4 IR 84984-83-15-481-B BC2F2:4 175 1300 4196 55 120 94.1 IR 84984-83-15-862-B BC2F2:4 238 1114 4018 58 121 94.1 Vandana 72 825 3556 54 120 Way Rarem 11 212 1610 81 122 B IR 90019:17-156-B BC3F2:3 522 1487 4712 61 106 98.3 IR 90019:17-159-B BC3F2:3 461 1930 5236 62 103 97.5 IR 90019:17-15-B BC3F2:3 565 2341 4534 65 107 98.3 IR 90020:22-265-B BC3F2:3 446 2090 4233 60 115 96.6 IR 90020:22-283-B BC3F2:3 415 1224 5950 58 100 94.9 Vandana 179 1049 4061 56 104 Way Rarem 0.1 500 2878 81 103 Dixit, Shalabh, IRRI
- 19. Differences in graintype of donor parent (Way Rarem), Recipient parent (Vandana), NIL (IR90019:17- 156-B) and pre NIL (IR90019:17-15-B)
- 20. Future needs • • Highthroughput, automated and cheaper genotyping services • Enough information and support to apply new breeding methodologies • Large scale drought screening facilities in wet season • Enough support to disseminate the newly released varieties
- 22.