Core principle Target of vaccine Blocking fusion proteins Steps of vaccine design Testing Results

1. S U P E R V A C C I N E F O R A L L
T Y P E S O F C O R O N A V I R U S
BY A S I F N AWA Z
M P H I L ( M I C R O B I O LO G Y ) 4 T H
AU S T

2. TALK SEQUENCE
• Core principle
• Target of vaccine
• Blocking fusion proteins
• Steps of vaccine design
• Testing
• Results

3. SUPER VACCINE
• Inexpensive
• Produced in 2 to 3 weeks
• Target: Non mutant, constant but critical in function
• Against all strains and variants
• Covers all corona virus
• Mild infection
• Old technology

4. STABLE AND CRITICAL PART OF VIRUS
• Fusion protein of S2 domain on spike.
• 93% constant
• Phagocytosed virus

5. BLOCKED FUSION PROTEIN
• Virus is viable but can’t get enter into cell

6. VACCINE DESIGN
• First step:
• Modified E.coli
• Bacteria is harmless
• Tokyo metropolitan university
• Slow growth
• Less surface proteins
• Plasmid of interest is inserted

7. AUTOTRANSPORTERS
• Second step
• Channels that bring up protein of interest to express on surface
• Modified transporter having SARS COV-2 fusion proteins
• Antigen presented
• Immune response/ attack.

8. PLASMIDS
• Third step
• Create plasmids to express transporters
• Two types
1. Produce SARS COV-2 fusion protein
2. Produce Alpha corona virus (PEDV)

9. CONTINUED..
Fourth step
• Add plasmid to bacteria
Fifth step
• Bottle up vaccine

10. SIXTH STEP
Testing
Group 1: SARS COV-2 fusion protein Vaccine
Group 2: Alpha corona
Group 3: Control

11. CHALLENGE WITH ANTIGEN
• Seventh step
• Covid 19 exposure to piglets
• All protected
• No severe disease

12. RESULTS
• Primarily T cell response
• Less B cells
• Pathway cytotoxic
• IFN gamma
• All strains covered up
• Cross protection
• No sterilization

13. REFERENCES
• https://www.pnas.org/content/118/18/e2025622118
• https://weather.com/en-IN/india/coronavirus/news/2021-04-21-scientists-invent-
new-vaccine-that-can-fight-all-coronaviruses

14. THANKS