Shilpy Joshi is a cancer biologist seeking a position in clinical cancer research and drug development. She has over 6 years of postdoctoral experience working with human cancer samples and mouse models. Her research has focused on cancer metabolism and autophagy, including projects elucidating metabolic differences between kidney tumor and normal cells, and studying the effects of mitochondrial dysfunction on tumorigenesis. She has strong skills in molecular biology, cell culture, animal studies, and collaborative multi-institute projects.

1. Shilpy Joshi, Ph.D
84 Cedar Lane, Apt-A, Highland Park, New Jersey 08904
(732) 289-7262, shilpy2010@hotmail.com; joshis1@cinj.rutgers.edu
Work Eligibility- United States Permanent Resident (Green Card)
Objective:
I am a driven, organized and innovative multidisciplinary cancer researcher who can rapidly understand complex
information in order to drive scientific results. I am a Cancer biologist by training with extensive expertise in
dealing of human patient samples, primary cells, cancer cell lines and mouse tumor models. I have broad
experience in running multiple collaborative projects to successful completion. Objective is to implement the
knowledge and the expertise for clinical cancer research and drug development.
Profile:
• 6+ years Postdoctoral experience
• Accomplished Cancer biologist with focus on translational cancer research, metabolism and autophagy
• Strong background in working with human cancer patient samples, primary cells, cancer cell lines, mouse
tumor models, 3D cultures
• Extensive experience in isotope tracer studies, Seahorse OCAR & ECAR and mitochondrial ETC enzymatic
assays
• Comprehensive training in CRISPR-Cas9(n), RNAi & shRNA (lentiviral) genome editing
• Preclinical drug efficacy studies in lung tumor mouse model
• Validation of targets for tumorigenesis/autophagy modulation
• Hybridoma, T cell response, cytokine profiling
• Presented research at scientific meetings, wrote grants, published scientific papers in peer reviewed journals
• Mentored junior scientists
Professional Experience:
Research Associate at Rutgers Cancer Institute of New Jersey (RCINJ), New Jersey, June 2015-present,
Mentor: Dr. Eileen White; resulting in an oral presentation award in NJCCR/RCINJ, an oral
presentation in Tristate Cancer Metabolism meeting, Princeton University, NJ
• Elucidation of metabolic differences between human renal oncocytoma vs normal kidney cells (in
collaboration with Joshua Rabinowitz lab, Princeton, NJ) : Ongoing project
• Effect of mitochondrial respiration defects on tumorigenesis by means of chemical inhibition
and/or Crispr KO of electron transport chain components : Ongoing project
NJCCR Postdoctoral fellow at RCINJ, New Jersey, May 2013-May 2015, Mentor: Dr. Eileen White;
resulting in travel grant award from International Keystone Symposia & a first author publication in
Cell Reports
• Genomic and biochemical analysis of human renal oncocytoma (in collaboration with MSKCC,
New York) : Completed project

2. Postdoctoral fellow at RCINJ, New Jersey, Feb 2011-May 2013, Mentor: Dr. Eileen White; resulting in
a 2 year NJCCR grant award of $100,000; Gallo award for scientific excellence by NJCCR/RCINJ ; first
author publication in Oncogene
• 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (PFKFB4) as a novel autophagy
regulator (in collaboration with Abraham lab, Pfizer and David Sabatini lab, MIT) :
Completed project
Education:
Ph.D. in Cell Biology and Immunology from Institute of Microbial Technology, Chandigarh, affiliate
of Jawaharlal Nehru University, New Delhi, India, 2003-2008
• Studies on cell envelope components of Mycobacteria: immunology of host pathogen interactions
Technical Skills:
• Models: Human patient samples, primary cell cultures, human/ mouse cancer cell lines, 3D- matrigel cultures,
Hybridoma, mouse tumor models.
• Cell biology: Seahorse (OCAR/ECAR), Flow cytometry, Incucyte, clonogenic, viability, cell based
reporter/functional assays, autophagy flux assays, drug treatment, Immunofluorescence assay.
• Molecular Biology: CRISPR-Cas9 mediated genome editing, RNAi lentiviral mediated stable knockdown of
targets, cloning, PCR and other general molecular biology techniques.
• Protein Biochemistry: Protein engineering, Purification, SDS PAGE, WB, IP, ChIP, ELISA, mitochondrial
enzymatic assays.
• Immunology: Hybridoma, T cell response, cytokine profiling
• Tissue analysis: Immunohistochemistry and Co-Immunostaining.
• Animal training: Adenocre nasal inhalation, oral gavage, subcutaneous, intraperitoneal injections, allograft
tumor assays, genotyping, pre-clinical drug efficacy studies in mice.
Professional skills:
§ Excellent analytical and problem-solving skills, team leader, innovative & persuasive.
§ Carried out independent/multi-institute collaborative projects to successful completion, mentored
junior scientists.
§ Well-organized in data collection, documentation (dossier/s) and good project management
strategies.
§ Good oral skills, presented research at national/international scientific meetings, wrote grants,
published scientific papers in peer- reviewed journals.
Most Recent peer-reviewed publications:
1. Kumar, N., Srivillibhuthur, M., Joshi, S., Walton, K. D., Zhou, A., Faller, W.J., Perekatt, A.O., Sansom, O. J.,
Gumucio, D.L., Xing, J., Bonder, E.M., Gao, N., White, E. and Verzi, M. (2016). A YY1-dependent increase in
aerobic metabolism is indispensable for intestinal organogenesis. Development. Manuscript accepted for
publication.
2. Joshi, S*., Tolkunov, D*., Aviv, H., Hakimi, A. A., Yao, M., Karsli-Uzunbas, G., Hsieh, J. J., Ganesan, S., Chan, C.
S., White, E (2015). The Genomic Landscape of Renal Oncocytoma Identifies a Metabolic Barrier to
Tumorigenesis. Cell Reports, Published online: November 19, 2015 (*Authors have equal contribution)
3. Strohecker, A.M*., Joshi, S*., Possemato, R., Abraham, R.T., Sabatini, D.M., White, E (2015). Identification of 6-
phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB4) as a Novel Autophagy Regulator by High
Content shRNA Screening. Oncogene. 2015 Nov 5; 34 (45): 5662-76.doi: 10.1038/onc.2015.23 Epub 2015 Ma16.
(*Authors have equal contribution)