Pattarawut Sopha has extensive education and research experience in biological sciences. He received his Ph.D. in Biological Sciences from Nara Institute of Science and Technology in Japan, and holds an M.Sc. in Molecular Genetics and Genetic Engineering from Mahidol University in Thailand and a B.Sc. in Microbiology from Srinakarinwirot University in Thailand. His research has focused on protein homeostasis, the unfolded protein response, and ER protein quality control. He has worked as a postdoctoral researcher at the University of North Carolina studying ER stress responses.
Keynote presentation, 4th February 2015, León, México - part of the 2015 Genomics Research on Plant-Parasite Interactions to Increase Food Production UK-MX Workshop.
Keynote presentation, 4th February 2015, León, México - part of the 2015 Genomics Research on Plant-Parasite Interactions to Increase Food Production UK-MX Workshop.
Dr. Ravi S Pandey-Resume for Assistant Professor/ Research Scientist in Bioch...Dr. Swami Gyan Prakash
Dear Member of Recruiter Committee,
I am writing this letter for a position of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology. In brief, I am PhD in Medicinal Biochemistry and completed several successful projects as a Postdoctoral Scientist in different discipline of Biochemistry like Molecular Biology of Aging, Alternative splicing in human diseases, Epigenetic regulation in liver and breast cancer from India and USA. I came back to India in April 2010 and worked as a CSIR-Pool Scientist in the area of chromatin remodeling in breast cancer. My tenure has been completed recently. Thus, I am looking for a faculty position of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology.
I am highly interested to trend the graduate students in Biochemistry, Biotechnology and Molecular Biology. Besides, I am very much motivated to lead projects in the area of Cancer Biology. Thus, I respectfully submit this letter of application, for I believe my experiences and commitment for teaching and research make me well qualified to meet the needs of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology.
I am well acquainted with the molecular techniques associated with DNA, RNA and proteins research. Besides, I am expertise in planning and execution of experiments, mentoring PhD students, interpreting data, as well as writing and data preparation for manuscript publication. During my doctorate and post doctorate time, I taught graduate and postgraduate students on behalf of my mentors. I am well organized, goal oriented; self motivated research scientist and committed to train the student in area of Clinical Biochemistry and Molecular Biology along with lead research work in the area of Cancer Biology. I would appreciate for an interview opportunity to discuss about my background, qualification and expertise that may fit for the position.
Nanoparticle of plant extract: A Novel approach for cancer therapyroshan telrandhe
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel Approach For Cancer Treatment. Sponsered By Indian Councial Of Medical Research, Delhi. OrganisedBy KamlaNehru College Of Pharmacy, Butibori, Nagpur441108, Maharastra, India. ON 25 & 26 March 2017
Spectroscopic Characterization of Biofield Treated Metronidazole and TinidazoleMahendra Kumar Trivedi
Metronidazole and tinidazole are widely used antimicrobial drugs against Gram-negative and Gram-positive anaerobic bacteria. The present study was aimed to evaluate the impact of biofield treatment on metronidazole and tinidazole using FT-IR and UV spectroscopy. The study was carried out in two groups i.e. control and treatment. Treatment groups were subjected to Mr. Trivedi’s biofield treatment while no treatment was given to control group. FT-IR spectrum of treated metronidazole showed the impact of biofield treatment on frequency of characteristic functional groups such as C=C (imidazole ring) stretching was appeared at lower frequency i.e. from 1600 cm-1 to 1553 cm-1. Likewise, NO2 asymmetric stretching and C-N symmetric stretching were appeared at higher wave number i.e. 1479 cm-1 to 1501 cm-1 and 1070 cm-1 to 1077 cm-1, respectively. FT-IR spectrum of tinidazole showed shifting in absorption peak of C-N stretching to higher wavenumber from 1002 cm-1 (control) to 1022 cm-1. The wavenumber of aromatic C=C bond (in imidazole) was shifted to lower frequency, which could be due to increases in conjugation effect. Further, increases in wavenumber of NO2 and C-N in treated sample suggested the increased force constant and bond strength as compared to control. Because of higher conjugation effect and increased bond strength, the molecule supposed to be more stable. The UV spectra of both metronidazole and tinidazole showed the similar patterns of lambda max (λmax) with respect to their control. The FT-IR results of both drugs suggest that there was an impact of biofield treatment on atomic level of metronidazole and tinidazole, as compared to control.
With the DNA sequences of more than 90 genomes completed, as well as a draft sequence of the human genome, a major challenge in modern biology is to understand the expression, function, and regulation of the entire set of proteins encoded by an organism—the aims of the new field of proteomics. This information will be invaluable for understanding how complex biological processes occur at a molecular level, how they differ in various cell types, and how they are altered in disease states. The term proteomics describes the study and characterization of a complete set of proteins present in a cell, organ, or organism at a given time.
In general, proteomic approaches can be used (a) for proteome profiling, (b) for comparative expression analysis of two or more protein samples, (c) for the localization and identification of posttranslational modifications, and (d) for the study of protein-protein interactions. The human genome harbours 26000–31000 protein-encoding genes; whereas the total number of human protein products, including splice variants and essential posttranslational modifications (PTMs), has been estimated to be close to one million. It is evident that most of the functional information on the genes resides in the proteome, which is the sum of multiple dynamic processes that include protein phosphorylation, protein trafficking, localization, and protein-protein interactions. Moreover, the proteomes of mammalian cells, tissues, and body fluids are complex and display a wide dynamic range of proteins concentration one cell can contain between one and more than 100000 copies of a single protein.
A rapidly emerging set of key technologies is making it possible to identify large numbers of proteins in a mixture or complex, to map their interactions in a cellular context, and to analyze their biological activities. Mass spectrometry has evolved into a versatile tool for examining the simultaneous expression of more than 1000 proteins and the identification and mapping of posttranslational modifications. High-throughput methods performed in an array format have enabled large-scale projects for the characterization of protein localization, protein-protein interactions, and the biochemical analysis of protein function. Finally, the plethora of data generated in the last few years has led to approaches for the integration of diverse data sets that greatly enhance our understanding of both individual protein function and elaborate biological processes.
POTENTIAL OF CURCUMIN IN PYRAMID CELL NECROSIS OF MICE (MUS MUSCULUS) DUE TO ...IAEME Publication
Mercury pollution in the aquatic environment can cause intoxication of marine
organisms and the formation of free radicals in the human body if consumed. Curcumin
is a natural ingredient that contains extracellular antioxidants to overcome the
formation of free radicals in the body. The purpose of the study was to determine the
effect of administering curcumin to the number of pyramid cells that were necrotic in
mice (Mus musculus) exposed to methylmercury. This experiment used a completely
randomized design with 4 groups of mice, i.e. 0.5 ml distilled water, 0.056 mg/kg
methylmercury, 0.0056 mg/kg methylmercury + 150 mg/kg curcumin and 0.0056 mg/kg
methylmercury + 300 mg/kg curcumin. Examination of total pyramid cell necrosis was
observed with a 400x magnification light microscope. In this study, there was a
decrease in the number of pyramid cell necrosis in mice. The decrease in the number
of necrotic cells was directly proportional to the increase in dose given. The treatment
results as positive controls showed the highest value with the number of pyramid cells
experiencing necrosis of 23.28. The highest decreases in the number of each treatment
were 10.32 in 150 mg/kgBW curcumin and 5.80 in 300 mg/kgBW curcumin. Curcumin
can reduce the number of pyramid cells that experience necrosis due to exposure to
methylmercury.
Dr. Ravi S Pandey-Resume for Assistant Professor/ Research Scientist in Bioch...Dr. Swami Gyan Prakash
Dear Member of Recruiter Committee,
I am writing this letter for a position of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology. In brief, I am PhD in Medicinal Biochemistry and completed several successful projects as a Postdoctoral Scientist in different discipline of Biochemistry like Molecular Biology of Aging, Alternative splicing in human diseases, Epigenetic regulation in liver and breast cancer from India and USA. I came back to India in April 2010 and worked as a CSIR-Pool Scientist in the area of chromatin remodeling in breast cancer. My tenure has been completed recently. Thus, I am looking for a faculty position of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology.
I am highly interested to trend the graduate students in Biochemistry, Biotechnology and Molecular Biology. Besides, I am very much motivated to lead projects in the area of Cancer Biology. Thus, I respectfully submit this letter of application, for I believe my experiences and commitment for teaching and research make me well qualified to meet the needs of Assistant Professor/ Research Scientist in Biochemistry, Clinical-Biochemistry, Biotechnology and Molecular Biology.
I am well acquainted with the molecular techniques associated with DNA, RNA and proteins research. Besides, I am expertise in planning and execution of experiments, mentoring PhD students, interpreting data, as well as writing and data preparation for manuscript publication. During my doctorate and post doctorate time, I taught graduate and postgraduate students on behalf of my mentors. I am well organized, goal oriented; self motivated research scientist and committed to train the student in area of Clinical Biochemistry and Molecular Biology along with lead research work in the area of Cancer Biology. I would appreciate for an interview opportunity to discuss about my background, qualification and expertise that may fit for the position.
Nanoparticle of plant extract: A Novel approach for cancer therapyroshan telrandhe
Presented in NATIONAL CONFERENCE ;A Phytomedicine : A Novel Approach For Cancer Treatment. Sponsered By Indian Councial Of Medical Research, Delhi. OrganisedBy KamlaNehru College Of Pharmacy, Butibori, Nagpur441108, Maharastra, India. ON 25 & 26 March 2017
Spectroscopic Characterization of Biofield Treated Metronidazole and TinidazoleMahendra Kumar Trivedi
Metronidazole and tinidazole are widely used antimicrobial drugs against Gram-negative and Gram-positive anaerobic bacteria. The present study was aimed to evaluate the impact of biofield treatment on metronidazole and tinidazole using FT-IR and UV spectroscopy. The study was carried out in two groups i.e. control and treatment. Treatment groups were subjected to Mr. Trivedi’s biofield treatment while no treatment was given to control group. FT-IR spectrum of treated metronidazole showed the impact of biofield treatment on frequency of characteristic functional groups such as C=C (imidazole ring) stretching was appeared at lower frequency i.e. from 1600 cm-1 to 1553 cm-1. Likewise, NO2 asymmetric stretching and C-N symmetric stretching were appeared at higher wave number i.e. 1479 cm-1 to 1501 cm-1 and 1070 cm-1 to 1077 cm-1, respectively. FT-IR spectrum of tinidazole showed shifting in absorption peak of C-N stretching to higher wavenumber from 1002 cm-1 (control) to 1022 cm-1. The wavenumber of aromatic C=C bond (in imidazole) was shifted to lower frequency, which could be due to increases in conjugation effect. Further, increases in wavenumber of NO2 and C-N in treated sample suggested the increased force constant and bond strength as compared to control. Because of higher conjugation effect and increased bond strength, the molecule supposed to be more stable. The UV spectra of both metronidazole and tinidazole showed the similar patterns of lambda max (λmax) with respect to their control. The FT-IR results of both drugs suggest that there was an impact of biofield treatment on atomic level of metronidazole and tinidazole, as compared to control.
With the DNA sequences of more than 90 genomes completed, as well as a draft sequence of the human genome, a major challenge in modern biology is to understand the expression, function, and regulation of the entire set of proteins encoded by an organism—the aims of the new field of proteomics. This information will be invaluable for understanding how complex biological processes occur at a molecular level, how they differ in various cell types, and how they are altered in disease states. The term proteomics describes the study and characterization of a complete set of proteins present in a cell, organ, or organism at a given time.
In general, proteomic approaches can be used (a) for proteome profiling, (b) for comparative expression analysis of two or more protein samples, (c) for the localization and identification of posttranslational modifications, and (d) for the study of protein-protein interactions. The human genome harbours 26000–31000 protein-encoding genes; whereas the total number of human protein products, including splice variants and essential posttranslational modifications (PTMs), has been estimated to be close to one million. It is evident that most of the functional information on the genes resides in the proteome, which is the sum of multiple dynamic processes that include protein phosphorylation, protein trafficking, localization, and protein-protein interactions. Moreover, the proteomes of mammalian cells, tissues, and body fluids are complex and display a wide dynamic range of proteins concentration one cell can contain between one and more than 100000 copies of a single protein.
A rapidly emerging set of key technologies is making it possible to identify large numbers of proteins in a mixture or complex, to map their interactions in a cellular context, and to analyze their biological activities. Mass spectrometry has evolved into a versatile tool for examining the simultaneous expression of more than 1000 proteins and the identification and mapping of posttranslational modifications. High-throughput methods performed in an array format have enabled large-scale projects for the characterization of protein localization, protein-protein interactions, and the biochemical analysis of protein function. Finally, the plethora of data generated in the last few years has led to approaches for the integration of diverse data sets that greatly enhance our understanding of both individual protein function and elaborate biological processes.
POTENTIAL OF CURCUMIN IN PYRAMID CELL NECROSIS OF MICE (MUS MUSCULUS) DUE TO ...IAEME Publication
Mercury pollution in the aquatic environment can cause intoxication of marine
organisms and the formation of free radicals in the human body if consumed. Curcumin
is a natural ingredient that contains extracellular antioxidants to overcome the
formation of free radicals in the body. The purpose of the study was to determine the
effect of administering curcumin to the number of pyramid cells that were necrotic in
mice (Mus musculus) exposed to methylmercury. This experiment used a completely
randomized design with 4 groups of mice, i.e. 0.5 ml distilled water, 0.056 mg/kg
methylmercury, 0.0056 mg/kg methylmercury + 150 mg/kg curcumin and 0.0056 mg/kg
methylmercury + 300 mg/kg curcumin. Examination of total pyramid cell necrosis was
observed with a 400x magnification light microscope. In this study, there was a
decrease in the number of pyramid cell necrosis in mice. The decrease in the number
of necrotic cells was directly proportional to the increase in dose given. The treatment
results as positive controls showed the highest value with the number of pyramid cells
experiencing necrosis of 23.28. The highest decreases in the number of each treatment
were 10.32 in 150 mg/kgBW curcumin and 5.80 in 300 mg/kgBW curcumin. Curcumin
can reduce the number of pyramid cells that experience necrosis due to exposure to
methylmercury.
Dedicated Research Scientist with experience in bacteriology and virology research and patient service. . Possessing excellent communication skills and able to work as a part of a team, with proven ability to work in tight schedules and adherence to deadlines. I grew keen interest in viral research during this period. Two research articles have been prepared by me during this period. One manuscript entitled," Prevalence of Hepatitis A and Hepatitis E in West Bengal, India: a tertiary care hospital based study" has been accepted in JEMDS and will be published in next issue.
The second one which is on dengue intervention is under consideration for publication in a very reputed journal.
My Ph.D. is on biomedical device associated biofilm infections where I have studied incidence, antibiotic tolerance of bacteria within biofilm and tried to find out some intervention strategies. I have published seven original research article in this topic and few others are in queue.
Bacteria within biofilm shows extremely high resistance towards antimicrobials used than its free floating counterparts (approx. 1000 fold). I would like to investigate the causes of this high resistance.
1. Pattarawut Sopha 2008-2012 Ph.D., Biological Sciences
Nara institute of science and technology, Japan
2002-2005 M.Sc., Molecular Genetics and Genetic Engineering
Mahidol University,Thailand
1998-2002 B.Sc., Microbiology
Srinakarinwirot University,Thailand
CurriculumVitae
Education
Program of Applied Biological Sciences,
Chulabhorn Graduate Institute
54 Kamphaeng Phet 6,Talat Bang Khen, Lak Si,
Bangkok 10210,THAILAND
pattarawut@cgi.ac.th, (+66)25541900 ext. 2643
Date of Birth: January 22nd, 1980
Nationality:Thai
Research Experience
DepartmentofBiology,FacultyofScience,
SrinakarinwirotUniversity,Bangkok,Thailand
Undergraduatestudent,Advisor:Dr.PrawatAungpraphapornchai
ThestudyofADIfragmentsofLactobacillusspp.
usingdegeneratedprimers
2001 - 2002
IMBG,MahidolUniversity,
NakornPathom,Thailand
Researchassistant,Advisor:Dr.WitoonTirasophon
CharacterizationofchimericyeastIre1pinSaccharomycescerevisiae
IMBG,MahidolUniversity,
NakornPathom,Thailand
Graduatestudent,Advisor:Dr.WitoonTirasophon
InvestigationofcatalyticpropertiesofhumanIre1αpinyeastmodel
DepartmentofBiochemistry,FacultyofScienceMahidolUniversity,
Bangkok,Thailand
Researchassistant,Advisor:Dr.MathurosePonglikitmongkol
Transcriptionalactivationanalysisofhumanpapillomavirus(HPV)oncoproteins(E6andE7),
mitochondrialDNAGeneticanalysisofThailandHornbills,
thestudyoftumorsuppressioneffectsinCurcumacomosaextracts
Instituteofmolecularbiologyandgenetics(IMBG),MahidolUniversity,
NakornPathom,Thailand
Researchassistant,Advisor:Dr.WitoonTirasophon
Thestudyofunfoldedproteinresponse(UPR)ininsectcell
underdenguevirus-persistentinfection
Narainstituteofscienceandtechnology,
Nara,Japan
Ph.D.student,Supervisor:Dr.KenjiKohno
StudyonanovelmammalianERtransmembraneJ-protein,DNAJB14
DepartmentofCellBiologyandPhysiology,SchoolofMedicine,
UniversityofNorthCarolina,ChapelHill,NorthCarolina,USA
PostdoctoralResearch,Supervisor:Dr.DouglasM.Cyr
Studyontheregulationofthetransmembrane
cheperonesunderacuteERstress
2003
2002 -2005
2005 - 2006
2006 - 2008
2008 - 2012
2013 - 2016
Protein Homeostasis,
Cell Stress Response and Regulation,
Endoplasmic Reticulum (ER) Protein Homeostasis,
Chaperone Expression and Function during Cell Stress,
Transmembrane Protein Quality Control,
Cancer Cell Stress Response
ResearchInterests
Lecturer/Researcher
2. References
Publications
The ER-transmembrane Hsp40 DNAJB12 is sensitive to acute ER stress and degraded before ER stressinduced
apoptosis. Pattarawut Sopha, HongYu Ren, and Douglas M. Cyr (Manuscript in prep.)
Restoration of R117H CFTR Folding and Function in Human Airway CellsThrough Combination
Treatment withVX-809 andVX-770. Martina Gentzsch, HongYu Ren, Scott A. Houck, Nancy L. Quinney, Deborah
M. Cholon, Pattarawut Sopha, Chaudhry IG, Jhuma Das, NikolayV. Dokholyan, Scott H. Randell, and Douglas M.
Cyr. Am J Physiol Lung Cell Mol Physiol. 2016. DOI: 10.1152/ajplung.00186.2016
VX-809 corrects folding defects in cystic fibrosis transmembrane conductance regulatorprotein through
action on membrane-spanning domain 1. HongYu Ren, Diane E. Grove, Oxana De La Rosa, Scott A. Houck,
Pattarawut Sopha, FredrickVan Goor, Beth J. Hoffman, and Douglas M. Cyr. Mol Biol Cell. 2013. 24(19): 3016-24.
A novel mammalian ER-located J-protein, DNAJB14, can accelerate ERAD of misfolded membrane
proteins. Pattarawut Sopha, Hiroshi Kadokura,Yo-heiYamamoto, MasatoTakeuchi, Michiko Saito, AkioTsuru,
and Kenji Kohno. Cell Struct. Funct. 2012. 37: 177-187.
Domain compatibility in Ire1 kinase is critical for the unfolded protein response. Juthakorn Poothong,
Pattarawut Sopha, Randal J. Kaufman, andWitoonTirasophon. FEBS Lett. 2010. 584: 3203 - 3208.
Isolation of lactic acid bacteria from fermented foods which have potential for tetramethylpyrazine
production. Prawat Aungpraphapornchai, Nat-hatai Sangobpun. and Pattarawut Sopha. Srinakarinwirot Sci. J.
2007. 23: 94 – 108
Presentation
Poster presentation:
The ER-transmembrane Hsp40 DNAJB12 mediates cross-talk between ERQC and apoptotic signaling
pathways to protect cells from acute proteotoxic stress. Federation of American Societies for Experimental Biology
(FASEB) – Summer Research Conferences (SRC) : From Unfolded Proteins in the Endoplasmic Reticulum to
Disease. June 2015 Saxtons river,Vermont, USA
Study of a novel mammalian transmembrane J-protein, DNAJB14. Federation of American Societies for
Experimental Biology (FASEB) – Summer Research Conferences (SRC) : From Unfolded Proteins in the
Endoplasmic Reticulum to Disease. June 2011 Saxtons river,Vermont, USA
in vivo characterization of chimeric yeast IRE1 in Saccharomyces cerevisiae. 29th Congress on Science and
Technology ofThailand. October 2003 Khonkaen University, Khonkaen,Thailand
Oral presentation:
ER Stress-dependent Degradation of theTransmembrane Hsp40 Sensitizes cells to Apoptosis. Cell Biology
and Physiology (CBP) Fusion Seminar. May 2015 UNC-Chapel Hill, NC, USA
Study of a novel transmembrane J-protein, DNAJB14. NAIST Global COE International StudentWorkshop
(Joint seminar: NAIST, UC-Davis, CAS-IGDB). November 2010 Osaka, Japan
Study of a novel transmembrane J-protein, DNAJB14.The Joint Seminar (NAIST-MU-NU) on Biosciences
& Biotechnology, February 2010 Mahidol University, Bangkok,Thailand
#1
Douglas M. Cyr, Professor of Cell Biology
University of North Carolina – Chapel Hill
Department of Cell Biology and Physiology, School of Medicine
Room 4312B, MBRB
Chapel Hill, NC 27516 USA
(+1) 919-843-4805
dmcyr@med.unc.edu
Relationship: Postdoc Supervisor
#2
Patrick J. Brenwald, Professor of Cell Biology
University of North Carolina – Chapel Hill
Department of Cell Biology and Physiology, School of Medicine
Room 4312C, MBRB
Chapel Hill, NC 27516 USA
(+1) 919-843-4995
pjbrennw@med.unc.edu
#3
Kadokura Hiroshi, Professor of Biochemistry
Tohoku University
2-1-1 Katahira, Aoba-ku, Sendai 980-8577 JAPAN
(+81) 022-217-5605
kadokura@tagen.tohoku.ac.jp
Relationship: PhD thesis committee
#4
Kohno Kenji, Professor of Molecular and Cell Genetics
Nara Institute of Science andTechnology,
8916-5 Ikoma, Nara 630-0192 JAPAN
(+81) 0743-72-5642
kkouno@bs.naist.jp
Relationship: Advisor
#5
Mathurose Ponglikitmongkol, Professor of Biochemistry
Mahidol University
Department of Biochemistry, Faculty of Science
272 Room B314, Rama 6 Rd., Ratchadevi, Bangkok 10400THAILAND
(+66) 2-201-5455
mathurose.pon@mahidol.ac.th
Relationship: Advisor
General molecular biology techniques e.g. gene cloning,
PCR and related applications; nucleotide analysis using
southern and northern blotting;protein purification and analysis;
bacterial, yeast and mammalian cell genetic manipulations;
polyclonal antibody production; Primary Lung Cell Culture
2001 Department of Biology, Faculty of Science,
Srinakarinwirot University, Bangkok,Thailand
Teaching assistant, Participating in laboratory lectures,
and preparing laboratory materials
2008 NAIST international scholarship
2004Thailand Pfizer foundation scholarship program for human
life science research.
Pfizer foundation, Bangkok,Thailand.
2003 The Royal Bangkok Sport Club (RBSC) scholarship for
graduate students.
The Royal Bangkok Sport Club, Bangkok,Thailand.
TheThailand Research Fund (TRF) granted from
Prof. Dr. Sakol Panyim.
The personal financial support from Prof. Dr. Sakol Panyim.
IMBG, Mahidol University, Nakorn Pathom,Thailand.
Scholarships
TeachingExperience
TechnicalSkills