EMD Millipore Corp. markets the SNAP i.d. 2.0 system, which uses vacuum-driven flow of reagents to streamline Western blotting and immunohistochemistry experiments. The system reduces handling time from hours to minutes by actively pulling reagents through membranes or slides, improving reagent distribution and binding. It allows processing of multiple blots or slides in parallel under consistent conditions. The document provides details on the system and demonstrates its ability to optimize antibody conditions and produce comparable results to traditional methods in a fraction of the time.
This document provides instructions for extracting DNA from a banana in a kitchen. The protocol involves:
1. Creating a saline solution and cutting the banana.
2. Adding the banana pieces to a plastic bag with saline, dish soap, and contact lens solution.
3. Mash the banana mixture and filter it into a test tube.
4. Transfer some of the filtered solution to a microtube using a pipette.
5. Add ethanol to the test tube and microtube to precipitate out the banana's DNA.
The document describes STRATEC Molecular's portfolio of nucleic acid purification kits and sample preparation solutions. It highlights the InviGenius automated nucleic acid purification system, which can process 12 samples directly from primary tubes to PCR-ready DNA/RNA. It also discusses the PSP SalivaGene DNA Kit for non-invasive saliva collection and DNA stabilization at room temperature for 12 months, and the PSP Spin Stool DNA Plus Kit for integrated stool sample collection, transport, and DNA purification.
Gel PCR/DNA fragments extraction kit(ydf) protocol v3.0 (1)BioStar Lifetech
This document provides instructions for using the HiYield Gel/PCR DNA Fragments Extraction Kit. It begins with safety precautions for handling reagents containing hazardous compounds. It then lists the contents of the kit and provides protocols for gel extraction and PCR clean up. The gel extraction protocol involves dissolving the gel slice, binding DNA to the column, washing, and eluting purified DNA. The PCR clean up protocol prepares the sample and then follows similar binding, washing, and elution steps. Troubleshooting tips are provided to address potential issues like low recovery, poor downstream performance, and difficult gel dissolution.
This document provides information on BioBall® strains that contain precise numbers of microorganisms for microbiological quality control testing. It lists various BioBall strains including their organism, strain identification, cell or spore type, number of tests, and shelf life. Strains include bacteria (e.g. E. coli, S. aureus), fungi (e.g. C. albicans, A. brasiliensis), and mixed kits for growth promotion, sterility assurance, and antimicrobial effectiveness testing. BioBall provides accredited reference materials within specified count ranges and standard deviations for reproducibility in microbiological tests.
chlorine, drinking water at home, elements, environment, filters for drinking water, greenfilter, health water, home water, industrial activities, ionicore salts during, light drinking water, light water, membrane, membranes, nanofiltration, osmosis, pure water, salts, salts rejection, semi-permeable.
We are a products, solutions and services wholesale supplier in water treatment. We provide only businesses companies such as distributors, retailers, manufacturers and assemblers.A catalog including carefully selected products, from the most prestigious brands in the Water Treatment market, operative website, personalized services, precise and efficient logistics, flexible organization, but also the basic importance that is attributed to human factors and relationships with partners, make of Sinergroup Srl a reference for many companies of the market. A continuous and consistent effort, the ability to anticipate the evolution of the market demand and orientation to the understanding and satisfaction of our customers has always characterizes us in our work. Born with the objective view in the exclusive distribution: Continue to be "Leader of the Brand Leader ", with activities and services of value. A choice of specialization that in recent years has allowed us to develop a unique knowledge and experience in the market. The thorough water treatment market understanding and high commercial performance, make us able to offer to our customers many products and services more personalized and appropriate to the individual end user. Our mission is to become your best business Partner!
chlorine, drinking water at home, elements, environment, filters for drinking water, greenfilter, health water, home water, industrial activities, ionicore salts during, light drinking water, light water, membrane, membranes, nanofiltration, osmosis, pure water, salts, salts rejection, semi-permeable.
We are a products, solutions and services wholesale supplier in water treatment. We provide only businesses companies such as distributors, retailers, manufacturers and assemblers.A catalog including carefully selected products, from the most prestigious brands in the Water Treatment market, operative website, personalized services, precise and efficient logistics, flexible organization, but also the basic importance that is attributed to human factors and relationships with partners, make of Sinergroup Srl a reference for many companies of the market. A continuous and consistent effort, the ability to anticipate the evolution of the market demand and orientation to the understanding and satisfaction of our customers has always characterizes us in our work. Born with the objective view in the exclusive distribution: Continue to be "Leader of the Brand Leader ", with activities and services of value. A choice of specialization that in recent years has allowed us to develop a unique knowledge and experience in the market. The thorough water treatment market understanding and high commercial performance, make us able to offer to our customers many products and services more personalized and appropriate to the individual end user. Our mission is to become your best business Partner!
This document provides instructions for extracting DNA from a banana in a kitchen. The protocol involves:
1. Creating a saline solution and cutting the banana.
2. Adding the banana pieces to a plastic bag with saline, dish soap, and contact lens solution.
3. Mash the banana mixture and filter it into a test tube.
4. Transfer some of the filtered solution to a microtube using a pipette.
5. Add ethanol to the test tube and microtube to precipitate out the banana's DNA.
The document describes STRATEC Molecular's portfolio of nucleic acid purification kits and sample preparation solutions. It highlights the InviGenius automated nucleic acid purification system, which can process 12 samples directly from primary tubes to PCR-ready DNA/RNA. It also discusses the PSP SalivaGene DNA Kit for non-invasive saliva collection and DNA stabilization at room temperature for 12 months, and the PSP Spin Stool DNA Plus Kit for integrated stool sample collection, transport, and DNA purification.
Gel PCR/DNA fragments extraction kit(ydf) protocol v3.0 (1)BioStar Lifetech
This document provides instructions for using the HiYield Gel/PCR DNA Fragments Extraction Kit. It begins with safety precautions for handling reagents containing hazardous compounds. It then lists the contents of the kit and provides protocols for gel extraction and PCR clean up. The gel extraction protocol involves dissolving the gel slice, binding DNA to the column, washing, and eluting purified DNA. The PCR clean up protocol prepares the sample and then follows similar binding, washing, and elution steps. Troubleshooting tips are provided to address potential issues like low recovery, poor downstream performance, and difficult gel dissolution.
This document provides information on BioBall® strains that contain precise numbers of microorganisms for microbiological quality control testing. It lists various BioBall strains including their organism, strain identification, cell or spore type, number of tests, and shelf life. Strains include bacteria (e.g. E. coli, S. aureus), fungi (e.g. C. albicans, A. brasiliensis), and mixed kits for growth promotion, sterility assurance, and antimicrobial effectiveness testing. BioBall provides accredited reference materials within specified count ranges and standard deviations for reproducibility in microbiological tests.
chlorine, drinking water at home, elements, environment, filters for drinking water, greenfilter, health water, home water, industrial activities, ionicore salts during, light drinking water, light water, membrane, membranes, nanofiltration, osmosis, pure water, salts, salts rejection, semi-permeable.
We are a products, solutions and services wholesale supplier in water treatment. We provide only businesses companies such as distributors, retailers, manufacturers and assemblers.A catalog including carefully selected products, from the most prestigious brands in the Water Treatment market, operative website, personalized services, precise and efficient logistics, flexible organization, but also the basic importance that is attributed to human factors and relationships with partners, make of Sinergroup Srl a reference for many companies of the market. A continuous and consistent effort, the ability to anticipate the evolution of the market demand and orientation to the understanding and satisfaction of our customers has always characterizes us in our work. Born with the objective view in the exclusive distribution: Continue to be "Leader of the Brand Leader ", with activities and services of value. A choice of specialization that in recent years has allowed us to develop a unique knowledge and experience in the market. The thorough water treatment market understanding and high commercial performance, make us able to offer to our customers many products and services more personalized and appropriate to the individual end user. Our mission is to become your best business Partner!
chlorine, drinking water at home, elements, environment, filters for drinking water, greenfilter, health water, home water, industrial activities, ionicore salts during, light drinking water, light water, membrane, membranes, nanofiltration, osmosis, pure water, salts, salts rejection, semi-permeable.
We are a products, solutions and services wholesale supplier in water treatment. We provide only businesses companies such as distributors, retailers, manufacturers and assemblers.A catalog including carefully selected products, from the most prestigious brands in the Water Treatment market, operative website, personalized services, precise and efficient logistics, flexible organization, but also the basic importance that is attributed to human factors and relationships with partners, make of Sinergroup Srl a reference for many companies of the market. A continuous and consistent effort, the ability to anticipate the evolution of the market demand and orientation to the understanding and satisfaction of our customers has always characterizes us in our work. Born with the objective view in the exclusive distribution: Continue to be "Leader of the Brand Leader ", with activities and services of value. A choice of specialization that in recent years has allowed us to develop a unique knowledge and experience in the market. The thorough water treatment market understanding and high commercial performance, make us able to offer to our customers many products and services more personalized and appropriate to the individual end user. Our mission is to become your best business Partner!
The document describes the BD FACSJazz cell sorter. It is a compact, affordable benchtop cell sorter that incorporates design features to simplify operation for researchers with limited flow cytometry experience. Key features include factory-optimized settings, intuitive alignment, real-time video monitoring, and BD FACS Accudrop technology. The BD FACSJazz requires less than 2 square feet of bench space and can be configured with up to three lasers and eight parameters to support a variety of applications. It aims to make commonly used cell sorting applications more accessible to researchers.
1. The document describes methods developed to assess brown rot resistance in peach fruits, including an orchard spray-based method and a laboratory drop-based method.
2. Phenotypic data on infection probability and rot progression was collected from multiple populations over two years using these methods. Sub-traits associated with resistance like cuticular conductance, stomatal density, and fruit size effects were also analyzed.
3. The results provide insights on factors influencing infection probability and rot progression to inform breeding for improved brown rot resistance by combining favorable sub-traits.
R-Water's TK60 disinfectant was tested by Microchem Laboratory and shown to be effective against common pathogens like Staphylococcus aureus and Pseudomonas aeruginosa with a rapid 60 second contact time. The disinfectant is generated on-site from salt, water and electricity, and regulated by the EPA as a pesticide device rather than a pesticide. It offers advantages over traditional disinfectants in terms of cost, convenience of on-site generation, and environmental friendliness.
Use of single-use technology in Aseptic processing of vaccines: Application s...Dr. Priyabrata Pattnaik
This document discusses the use of single-use technologies in aseptic processing of vaccines. It notes the growing adoption of these technologies driven by factors like new facility design, retrofitting existing operations, and a growing range of applications. Some key challenges with balancing product and operator safety are discussed. The document also covers regulatory requirements and considerations for validation of these systems. Examples of using single-use technologies for activities like formulation, filling, filtration and powder handling are provided.
This document provides safety information for Liqtro Extremo 10W motor oil. It contains additives to improve lubrication as well as refined mineral base oil. No significant health hazards are expected from exposure. In the event of skin contact, wash with soap and water; eye contact should be flushed for 15 minutes. The product is combustible and protective equipment should be worn in the event of a fire. Spills should be cleaned up with absorbent materials and disposal procedures followed. When handling and storing, containers should be kept closed in a cool, well-ventilated area.
Cowin Biotech is a Chinese biotech company founded in 2007 that provides molecular diagnostic products and services. The company has R&D centers in Jiangsu, Beijing, and Boston and produces over 1,000 products through independent research. Cowin's core capabilities include viral transport media, nucleic acid extraction kits, PCR reagents, and enzymes. It aims to provide a complete solution for clinical diagnostic applications.
This document provides guidance on what constitutes an annotated bibliography and how to write annotations. An annotated bibliography contains bibliographic citations followed by paragraphs summarizing each source. Annotations include a description of the source's main ideas, the author's credentials, intended audience, relevance to the topic, and how it compares to other sources. The document also shows a sample annotated citation in MLA format and suggests databases for finding author biographies.
This document provides instructions for submitting supplemental monthly detail information required for the Utah Municipal Telecommunications License Tax return. Filers must submit the information electronically in a comma-delimited file with a specific naming convention and file format. The file should be uploaded to a designated FTP site and subdirectory. Examples of properly formatted file records and instructions for filing amended returns are also provided.
The document is about various topics related to banking, investment, economy and finance in Georgia. It contains articles on the Georgian economy, World Bank activities in Georgia, banking sector trends, interviews with leaders of companies operating in Georgia like Finca Bank and Georgian Industrial Group. There are also sections on microfinance, insurance, investment opportunities, state budget details and economic indicators. The interview with Joerg Matthies of Georgian Industrial Group discusses their activities in areas like power generation, coal mining and real estate, and future plans to develop renewable energy and increase electricity exports.
Jean Pasfield has over 20 years of experience in management, administration, and project coordination roles, specializing in Aboriginal housing and community development. She holds qualifications in government management, project management, and Excel/Access. Her experience includes managing tenancy databases and funding as Business Development Manager for Mid Lachlan Aboriginal Housing Co-op, coordinating large projects at the Aboriginal Housing Office including a $36M housing program, and providing secretariat support for a national indigenous housing committee. She has strong communication, reporting, and database skills.
Addison Camomile has over one year of experience as a medical records specialist. She has excellent problem solving and communication skills, and is capable of helping to resolve issues with medical records and billing. Camomile has skills in Microsoft Office, EPIC software, and customer service.
This document is a resume for Brandon Jones providing his contact information, objective, work experience, education, and references. Jones has over 10 years of experience in maintenance, shipping, receiving, stocking, and merchandising roles. His most recent role was as a Maintenance and Shipping Coordinator at Keystone Management from 2012 to present. He seeks a stable position with a well-established organization.
El documento describe los problemas de drenaje y salinidad en la costa peruana. La mayoría de los problemas se deben a características geológicas y a causas antropogénicas como obras de drenaje incompletas o mal mantenidas. Se han perdido más de 90,000 hectáreas de tierras fértiles debido a la salinización. Se necesitan inversiones para mejorar los sistemas de drenaje y prevenir más pérdidas de tierras agrícolas.
Знаем Как и Искаме Да Ти Помогнем Да Постигнеш Мечтите Си! - Семинари Я. НиколовTeodora Ivanova
Абонирайте се сега и за безплатните ни обучения и TV канала ни в YouTube: http://bit.ly/YNVideo
Включете се в благотворителните ни кампании ФДБ:
http://bulgarianchildren.org
Уебсайтът на Ясен Николов и 7 дни сила е тук, посетете го:
http://yasennikolov.com
1. This document outlines BioHackathon 2015 which was held in Nagasaki, Japan.
2. It provides a brief history of Nagasaki and Nagasaki University, including its origins as a trading port and the establishment of its medical school.
3. It discusses challenges in gene hunting for rare and undiagnosed diseases even with new sequencing technologies, and proposes that semantic web technologies can help address these challenges by facilitating knowledge sharing across databases.
ENSAYO Ventajas y desventajas de los ambientes educativos para una misma herr...Gabriella Hernandez
Este documento describe las ventajas y desventajas de los ambientes educativos para el uso de herramientas digitales. Entre las ventajas se encuentran que las actividades son más motivadoras, los estudiantes aprenden a usar la tecnología y hay más atención. Las desventajas incluyen la falta de cobertura tecnológica, problemas técnicos y de seguridad, y barreras económicas. El documento concluye que aunque la tecnología tiene beneficios, también existen desafíos, y se requiere esf
Kapildeb Karmakar is an Indian national with over 5 years of experience in the automotive domain. He has expertise in requirements engineering, system testing, embedded software development, and validation engineering. Some of his technical skills include C/C++, CANoe, CAPL, DOORS, and Synergy. He has worked on projects involving instrument clusters, camera systems, and infotainment systems for clients such as GM, Valeo, Renault, and Ferrari. He holds a B.E. in Information Technology from Bengal Engineering & Science University.
This document provides instructions for configuring profitability analysis in SAP. It discusses defining characteristics, value fields, operating concerns, and other key structures. Characteristics represent dimensions for analysis, like customer or product, while value fields are the metrics like sales or costs. An operating concern defines which characteristics and value fields to use for a specific analysis. The document walks through creating new characteristics and value fields, then defining an operating concern with ID 9100 that uses the Middle Eastern currency. It also assigns a controlling area and activates the configuration. This provides the basic structures for profitability analysis in SAP.
Targeted resumes focus on highlighting an applicant's soft skills like communication, confidence, and teamwork in a profile summary and listing hard skills and professional history to pass applicant tracking systems and land more interviews. Tailoring resumes to specific jobs helps decision makers better understand an applicant's worth and increases the chances of employers contacting applicants for 2,100 resumes submitted per job each week.
The document describes the BD FACSJazz cell sorter. It is a compact, affordable benchtop cell sorter that incorporates design features to simplify operation for researchers with limited flow cytometry experience. Key features include factory-optimized settings, intuitive alignment, real-time video monitoring, and BD FACS Accudrop technology. The BD FACSJazz requires less than 2 square feet of bench space and can be configured with up to three lasers and eight parameters to support a variety of applications. It aims to make commonly used cell sorting applications more accessible to researchers.
1. The document describes methods developed to assess brown rot resistance in peach fruits, including an orchard spray-based method and a laboratory drop-based method.
2. Phenotypic data on infection probability and rot progression was collected from multiple populations over two years using these methods. Sub-traits associated with resistance like cuticular conductance, stomatal density, and fruit size effects were also analyzed.
3. The results provide insights on factors influencing infection probability and rot progression to inform breeding for improved brown rot resistance by combining favorable sub-traits.
R-Water's TK60 disinfectant was tested by Microchem Laboratory and shown to be effective against common pathogens like Staphylococcus aureus and Pseudomonas aeruginosa with a rapid 60 second contact time. The disinfectant is generated on-site from salt, water and electricity, and regulated by the EPA as a pesticide device rather than a pesticide. It offers advantages over traditional disinfectants in terms of cost, convenience of on-site generation, and environmental friendliness.
Use of single-use technology in Aseptic processing of vaccines: Application s...Dr. Priyabrata Pattnaik
This document discusses the use of single-use technologies in aseptic processing of vaccines. It notes the growing adoption of these technologies driven by factors like new facility design, retrofitting existing operations, and a growing range of applications. Some key challenges with balancing product and operator safety are discussed. The document also covers regulatory requirements and considerations for validation of these systems. Examples of using single-use technologies for activities like formulation, filling, filtration and powder handling are provided.
This document provides safety information for Liqtro Extremo 10W motor oil. It contains additives to improve lubrication as well as refined mineral base oil. No significant health hazards are expected from exposure. In the event of skin contact, wash with soap and water; eye contact should be flushed for 15 minutes. The product is combustible and protective equipment should be worn in the event of a fire. Spills should be cleaned up with absorbent materials and disposal procedures followed. When handling and storing, containers should be kept closed in a cool, well-ventilated area.
Cowin Biotech is a Chinese biotech company founded in 2007 that provides molecular diagnostic products and services. The company has R&D centers in Jiangsu, Beijing, and Boston and produces over 1,000 products through independent research. Cowin's core capabilities include viral transport media, nucleic acid extraction kits, PCR reagents, and enzymes. It aims to provide a complete solution for clinical diagnostic applications.
This document provides guidance on what constitutes an annotated bibliography and how to write annotations. An annotated bibliography contains bibliographic citations followed by paragraphs summarizing each source. Annotations include a description of the source's main ideas, the author's credentials, intended audience, relevance to the topic, and how it compares to other sources. The document also shows a sample annotated citation in MLA format and suggests databases for finding author biographies.
This document provides instructions for submitting supplemental monthly detail information required for the Utah Municipal Telecommunications License Tax return. Filers must submit the information electronically in a comma-delimited file with a specific naming convention and file format. The file should be uploaded to a designated FTP site and subdirectory. Examples of properly formatted file records and instructions for filing amended returns are also provided.
The document is about various topics related to banking, investment, economy and finance in Georgia. It contains articles on the Georgian economy, World Bank activities in Georgia, banking sector trends, interviews with leaders of companies operating in Georgia like Finca Bank and Georgian Industrial Group. There are also sections on microfinance, insurance, investment opportunities, state budget details and economic indicators. The interview with Joerg Matthies of Georgian Industrial Group discusses their activities in areas like power generation, coal mining and real estate, and future plans to develop renewable energy and increase electricity exports.
Jean Pasfield has over 20 years of experience in management, administration, and project coordination roles, specializing in Aboriginal housing and community development. She holds qualifications in government management, project management, and Excel/Access. Her experience includes managing tenancy databases and funding as Business Development Manager for Mid Lachlan Aboriginal Housing Co-op, coordinating large projects at the Aboriginal Housing Office including a $36M housing program, and providing secretariat support for a national indigenous housing committee. She has strong communication, reporting, and database skills.
Addison Camomile has over one year of experience as a medical records specialist. She has excellent problem solving and communication skills, and is capable of helping to resolve issues with medical records and billing. Camomile has skills in Microsoft Office, EPIC software, and customer service.
This document is a resume for Brandon Jones providing his contact information, objective, work experience, education, and references. Jones has over 10 years of experience in maintenance, shipping, receiving, stocking, and merchandising roles. His most recent role was as a Maintenance and Shipping Coordinator at Keystone Management from 2012 to present. He seeks a stable position with a well-established organization.
El documento describe los problemas de drenaje y salinidad en la costa peruana. La mayoría de los problemas se deben a características geológicas y a causas antropogénicas como obras de drenaje incompletas o mal mantenidas. Se han perdido más de 90,000 hectáreas de tierras fértiles debido a la salinización. Se necesitan inversiones para mejorar los sistemas de drenaje y prevenir más pérdidas de tierras agrícolas.
Знаем Как и Искаме Да Ти Помогнем Да Постигнеш Мечтите Си! - Семинари Я. НиколовTeodora Ivanova
Абонирайте се сега и за безплатните ни обучения и TV канала ни в YouTube: http://bit.ly/YNVideo
Включете се в благотворителните ни кампании ФДБ:
http://bulgarianchildren.org
Уебсайтът на Ясен Николов и 7 дни сила е тук, посетете го:
http://yasennikolov.com
1. This document outlines BioHackathon 2015 which was held in Nagasaki, Japan.
2. It provides a brief history of Nagasaki and Nagasaki University, including its origins as a trading port and the establishment of its medical school.
3. It discusses challenges in gene hunting for rare and undiagnosed diseases even with new sequencing technologies, and proposes that semantic web technologies can help address these challenges by facilitating knowledge sharing across databases.
ENSAYO Ventajas y desventajas de los ambientes educativos para una misma herr...Gabriella Hernandez
Este documento describe las ventajas y desventajas de los ambientes educativos para el uso de herramientas digitales. Entre las ventajas se encuentran que las actividades son más motivadoras, los estudiantes aprenden a usar la tecnología y hay más atención. Las desventajas incluyen la falta de cobertura tecnológica, problemas técnicos y de seguridad, y barreras económicas. El documento concluye que aunque la tecnología tiene beneficios, también existen desafíos, y se requiere esf
Kapildeb Karmakar is an Indian national with over 5 years of experience in the automotive domain. He has expertise in requirements engineering, system testing, embedded software development, and validation engineering. Some of his technical skills include C/C++, CANoe, CAPL, DOORS, and Synergy. He has worked on projects involving instrument clusters, camera systems, and infotainment systems for clients such as GM, Valeo, Renault, and Ferrari. He holds a B.E. in Information Technology from Bengal Engineering & Science University.
This document provides instructions for configuring profitability analysis in SAP. It discusses defining characteristics, value fields, operating concerns, and other key structures. Characteristics represent dimensions for analysis, like customer or product, while value fields are the metrics like sales or costs. An operating concern defines which characteristics and value fields to use for a specific analysis. The document walks through creating new characteristics and value fields, then defining an operating concern with ID 9100 that uses the Middle Eastern currency. It also assigns a controlling area and activates the configuration. This provides the basic structures for profitability analysis in SAP.
Targeted resumes focus on highlighting an applicant's soft skills like communication, confidence, and teamwork in a profile summary and listing hard skills and professional history to pass applicant tracking systems and land more interviews. Tailoring resumes to specific jobs helps decision makers better understand an applicant's worth and increases the chances of employers contacting applicants for 2,100 resumes submitted per job each week.
The document outlines an 8-phase process for appropriately tuning automated transaction monitoring scenarios to balance identifying suspicious activity while maximizing efficiency. It involves using statistical techniques like clustering to segment transaction data into groups accounting for outliers. Transaction parameters are translated into "events" across clusters to establish threshold bands for qualitative assessment. The goal is to set an initial baseline threshold at the 85th percentile to define normal vs unusual activity, then refine it through investigation.
This document introduces several automated nucleic acid isolation systems produced by Solison Biotechnology Corp. The SolMag® series uses magnetic bead separation and liquid handling automation to quickly purify nucleic acids from biospecimens. The core technologies use switchable magnetic bead surfaces and nano-scale modifications to optimize sample collection and extraction. The SolMag®12 can process 1-12 samples simultaneously in 35 minutes while the SolMag®24 increases throughput to 24 samples per run. Both systems offer pre-filled reagent cartridges and easy to use touchscreen interfaces.
This document introduces several automated nucleic acid isolation systems produced by Solison Biotechnology Corp. The SolMag® series uses magnetic bead separation and liquid handling automation to quickly purify nucleic acids from biospecimens. The core technologies use switchable magnetic bead surfaces and nano-scale modifications to optimize sample collection and extraction. The SolMag®12 can process 1-12 samples simultaneously in 35 minutes while the SolMag®24 increases throughput to 24 samples per run. Both systems offer pre-filled reagent cartridges and easy to use touchscreen interfaces.
The BenchMark Special Stains Product Guide includes product specific information about BenchMark Special Stains kit components and reagents, a visual demonstration of the protocol options for each assay, and information about optimizing staining performance for the BenchMark Special Stains automated staining platform.
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
The ThinPrep 5000 processor is a fully automated system that can process up to 20 gynecological or cytology samples per batch. It utilizes proven ThinPrep technology to disperse, collect, and transfer cells from patient samples onto microscope slides in a controlled manner. The system ensures chain-of-custody by matching barcodes between each sample vial and slide to avoid mix-ups.
This document provides information on immunohistochemistry (IHC), including:
- IHC is a method to identify specific antigens within tissue sections using antigen-specific antibodies.
- Key steps include preparing the sample, retrieving the antigen, blocking background, detecting the target, and visualizing the sample under a microscope.
- It discusses common IHC reagents like DAB kits, primary and secondary antibodies, and buffers, as well as the basic procedure for running IHC on the BenchMark XT automated slide stainer.
Leica BOND-III is a fully automated IHC and ISH stainer that provides quality, speed, and efficiency. It connects easily to common laboratory information systems and sample tracking. A range of Novocastra reagents are optimized for it. It offers unrivaled service and support programs. The stainer reduces work and potential errors for improved diagnostic confidence and patient care.
The document describes Zepto, an innovative disposable device for cataract surgery that uses precision-pulse technology to create an accurate and reproducible capsulotomy quickly and safely. It discusses studies showing Zepto results in minimal temperature change in the eye and stronger capsulotomy edges compared to other methods. The document also outlines Dymedex's market analysis projecting rapid adoption of Zepto as it addresses the needs of high-risk patient populations and its ease of use could make it the standard of care.
BioStar Lifetech Genomic DNA extraction kit(rbp96 b) protocolBioStar Lifetech
This document provides protocols for using a 96-well genomic DNA extraction kit to purify DNA from blood, tissues, and other samples in a high-throughput format. The kit uses proteinase K and chaotropic salts to lyse cells and degrade proteins, then binds DNA to a glass fiber plate. DNA is washed and eluted in less than an hour for use in downstream applications such as PCR. The document describes vacuum and spin column protocols for blood, tissues, and other sample types.
The GeneDisc Rapid Microbiology System provides microbial test results in as little as two hours using quantitative PCR technology. The system consists of a DNA Extractor that prepares samples, GeneDisc plates that are loaded into a GeneDisc Cycler for analysis. It tests for key pathogens in biopharmaceuticals, foods, and water. The system offers simplicity, speed, security of results, and cost savings compared to traditional methods.
The STA Compact Max 3 coagulation analyzer offers improved performance through its EPC module, which detects hemolyzed, icteric, and lipemic samples to ensure quality results without additional sample volume or affecting throughput. It can process up to 96 capped, uncapped, or microtubed samples randomly for efficient STAT management and fast turnaround times. The STA Compact Max 3 also simplifies reagent management with 45 cooled positions, pre-calibration functions, and the ability to manage multiple reagent lots simultaneously.
This document provides an overview of the molecular diagnostics market and SolMag aNAPs, an automated nucleic acid purification system. The molecular diagnostics market is growing rapidly due to factors like increasing infectious diseases and cancer. PCR and real-time PCR represent the majority of the molecular diagnostic market. SolMag aNAPs is an automated system that uses magnetic bead technology to quickly purify nucleic acids from samples in 1-12 samples simultaneously in under 45 minutes. Its advantages over competitors include being lighter weight, lower cost per sample, and the ability to integrate with downstream automation. The target markets for SolMag aNAPs are hospitals, research institutes, and countries with high infectious disease rates.
Athenese-Dx specializes in the research, development, manufacture, and marketing of in vitro diagnostic tests and Instruments. Our state-of-the-art facility is based in Chennai, India. Continuous innovation drives us forward. Our dedicated scientists are developing high quality IVD tests in Clinical Chemistry, Rapid Test, ELISA, Fluorescence Immunoassay, Molecular Diagnostics, and Instruments. Our R&D Unit is recognized by the Department of Scientific and Industrial Research (DSIR)
ADX has grown from “ importing for India”, “Made in India for India”, to “Made in India for world”. Our ultimate goal is to bring innovative diagnostic tests at the highest quality to the world market. We reach our customers by combining the skills of our sales force with a network of distributors and strategic partners. We have proven track records of delivering quality products and empowering clinicians to make healthcare decision faster and more accurate.
ShuntCheck is an aid to the detection of flow in implanted cerebrospinal fluid (CSF) shunts. ShuntCheck includes Micro-Pumper, a component which may be used to temporarily increase CSF flow in suspected non-flowing, patent shunts during the ShuntCheck test. ShuntCheck cannot alone diagnose CSF shunt function or malfunction. The clinical diagnosis of CSF shunt function or malfunction, incorporating the flow information from ShuntCheck, should be made only by a qualified neurosurgeon.
Parvovirus Filtration Best Practices - 25 Years of Hands-On ExperienceMilliporeSigma
In this webinar, you will learn:
- how to measure filter performance and capacity,
- how to optimize filter virus removal capability,
- and avoid potential pit-falls
Detailed description:
This webinar will cover all aspects of parvovirus filtration best practices: process development/ optimization, pilot scale-up, and validation and explain the important connections between these activities. The rationale for the recommended best practices will be explained by discussing the underlying mechanisms that control filter performance.
Parvovirus Filtration Best Practices - 25 Years of Hands-On ExperienceMerck Life Sciences
In this webinar, you will learn:
- how to measure filter performance and capacity,
- how to optimize filter virus removal capability,
- and avoid potential pit-falls
Detailed description:
This webinar will cover all aspects of parvovirus filtration best practices: process development/ optimization, pilot scale-up, and validation and explain the important connections between these activities. The rationale for the recommended best practices will be explained by discussing the underlying mechanisms that control filter performance.
This document provides instructions for performing a peptide radioimmunoassay (RIA). It describes the basic principles and components of the RIA kits including a radiolabeled tracer, standards, antiserum, and secondary antibodies. Detailed multi-day protocols are provided for setting up the assay, including reconstituting and diluting components, incubating samples and standards with antiserum and tracer, adding secondary antibodies and serum, and counting and analyzing results. Safety precautions are also described when handling radioactive materials.
This document discusses using photo-controlled chemicals to direct cardiac tissue growth and function. Specifically, it examines:
1) Using azobenzene derivatives that suppress cardiac excitation in response to light, allowing control of propagation speed and patterning of waves.
2) Creating engineered cardiac tissues on nanofiber scaffolds for alignment and precise positioning of cells.
3) Developing 3D cardiac tissues and whole heart preparations that demonstrate reversible light-controlled excitation, as well as tissues derived from pluripotent stem cells.
20160219 - M. Agostini - Nuove tecnologie per lo studio del DNA tumorale libe...Roberto Scarafia
Nano Inspired Biomedicine Laboratory
1 Department of Surgical, Oncological and Gastroenterological Sciences, University of Padua, Italy.
2 Istituto di Ricerca Pediatrica- Città della Speranza, Padova, Italy.
20160219 - M. Agostini - Nuove tecnologie per lo studio del DNA tumorale libe...
SNAP 2 Brochure
1. EMD Millipore Corp. is a subsidiary of Merck KGaA, Darmstadt, Germany
Just SNAP and go!
SNAP i.d.®
2.0 system for
Western blotting and IHC
2. There’s so much room for experimental variability in traditional
immunodetection workflows. For your peace of mind – and ours –
we designed the SNAP i.d.® 2.0 system to streamline your Western
blot and immunohistochemistry experiments.
The concept is simple: a vacuum-driven flow of blocking, antibody,
and washing solutions reduces slide and membrane handling. That
means a lot less shaking, dipping, pouring and waiting.
And now you can process multiple blots and slides in parallel, so it’s
easy to apply consistent conditions across experiments.
Take protein detection to new dimensions: www.emdmillipore.com/SNAP
2
Multiple slides, multiple blots,
multiple conditions.
3. 3
SNAP i.d.®
2.0 Protein Detection
System for Western Blotting
Unlike conventional Western blotting, where diffusion is the
primary means of reagent transport, the SNAP i.d.® 2.0
system applies a vacuum to actively drive reagents through
the membrane. This advanced technology promotes antigen
binding and thorough washing, enabling you to better
optimize your Western blotting conditions.
Key Benefits
• Faster results
• Faster testing of different antibodies
• Higher throughput of Western blots each day
How does the SNAP i.d.® 2.0 system work?
The vacuum-driven SNAP i.d.® 2.0 system fully exploits
three-dimensional reagent distribution and decreases the
immunodetection time from hours to minutes using the
following mechanisms:
1. The system increases local antibody concentrations
at binding sites by using vacuum filtration, driving
the antibody – antigen binding reaction forward and
shortening incubation times.
2. Vacuum pulls any residual, unbound antibody out of
the membrane, lowering background signal.
Advantages of the SNAP i.d.® 2.0 System’s
vacuum transport feature
• Draws reagents through blotting membrane
• Minimizes over-blocking
• Thoroughly flushes membranes instead of just rinsing
• Reduces incubation times
Faster Blots, Better
Signals. Comparison of the
traditional Western blotting
protocol relative to SNAP
i.d.®
2.0 system’s 30 minute
protocol.
SNAP i.d.®
2.0 system in the Western blotting workflow
SNAP i.d.® system actively
pulls the reagents through
the membrane
Traditional Western blotting
relies on diffusion
Antigen
embedded in
membrane
Antigen
trapped in
membrane
SNAP i.d.®
2.0 Steps:
30 min
Sample Prep
= Wash
Electrophoresis Membrane Transfer Blocking
Primary Antibody
Secondary Antibody
Detection
4. 4
Equivalent performance, a fraction of the time.
You frequently need to optimize antibody conditions using multiple antibody dilutions, across multiple sample types
and matrices. With the SNAP i.d.® 2.0 system, you can turn around your optimization experiments in a fraction of the
time it takes for traditional Western blot detection.
Human brain samples from an Alzheimer’s disease patient
and from a healthy donor were lysed in CytoBuster™
Protein Extraction Reagent (Cat. No. 71009). Samples
were serially diluted and separated by SDS-PAGE. Gels
were transferred to Immobilon®-P membrane. Blots were
processed in the SNAP i.d.® 2.0 system using MultiBlot,
Mini, and Midi frames with their corresponding blot
MultiBlot, Mini Blot,
and Standard Western blot detection
Lane Concentration (μg)
1 20
2 10
3 5
4 2.5
5 1.25
Midi Blot
Lane Concentration (μg)
1 20
2 10
3 5
4 2.5
5 1.25
6 0.63
7 0.31, 0.16 and 0.08
8 0.16
9 0.08
SNAP i.d.® 2.0 MultiBlot SNAP i.d.® 2.0 Mini Blot SNAP i.d.® 2.0 Midi Blot
Standard Western blot
detection
MW
Blocking 0.5% NFDM for 20 sec 0.5% NFDM for 20 sec 0.5% NFDM for 20 sec 0.5% NFDM for 1 hr
Primary
Antibody
Anti-Tau1 1: 1,000 for
10 min
Anti-Tau1 1: 1,000 for
10 min
Anti-Tau1 1: 1,000 for 10 min Anti-Tau1 1: 5,000 for 1 hr
Secondary
Antibody
Goat anti-Mouse 1: 10,000
for 10 min
Goat anti-Mouse 1: 10,000
for 10 min
Goat anti-Mouse 1: 10,000 for 10 min Goat anti-Mouse 1: 50,000
for 1 hr
Total Time 30 min 30 min 30 min 3 hr 30 min
Western blot optimization of anti-Tau-1 antibody in Alzheimer’s disease brain and healthy brain samples
Alzheimer’s brain Healthy brain
188
98
62
49
38
28
14
6
3
1 2 3 4 5 1 2 3 4 5
Alzheimer’s brain Healthy brain
188
98
62
49
38
28
14
6
3
1 2 3 4 5 1 2 3 4 5
Alzheimer’s brain Healthy brain
188
98
62
49
38
28
14
6
3
1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9
Alzheimer’s brain Healthy brain
188
98
62
49
38
28
14
6
3
1 2 3 4 5 1 2 3 4 5
Blot Holder Specifications
Midi Mini Each half of MultiBlot
Dimensions (cm) 17.8 x 10.2 12.7 x 9.1 11.4 x 6.4
Max Blot Size (cm) 8.5 x 13.5 7.5 x 8.4 4.5 x 8.5
holders. A control blot was processed by standard
immunodetection. All blots were blocked with 0.5% NFDM
and probed with primary anti-Tau1 (Cat. No. MAB3420)
and secondary HRP-conjugated goat anti-mouse
(Cat. No. AP124P) using the conditions indicated above.
Blots were incubated with Luminata™ Forte HRP substrate
and exposed to X-ray film for 15 minutes.
5. 5
Product Description Cat. No. Qty/Pk
Base System
The SNAP® i.d. 2.0 Systems contain everything you need to get started, including the detection base, 2 blot holding frames, 2 blot
holders, 2 antibody collection trays, a blot roller and rolling pad, 2 wetting trays, vacuum tubing, and a Quick Start User Guide.
SNAP® i.d. 2.0 System - Mini (7.5 x 8.4 cm) SNAP2MINI 1
SNAP® i.d. 2.0 System - Midi (8.5 x 13.5 cm) SNAP2MIDI 1
SNAP® i.d. 2.0 System - MultiBlot (4.5 x 8.4 cm) SNAP2MB3 1
SNAP® i.d. 2.0 System - Mini and Midi (7.5 cm and 8.5 x 13.5 cm) SNAP2MM 1
SNAP® i.d. 2.0 System - Mini and MultiBlot SNAP2MB1 1
SNAP® i.d. 2.0 System - Midi and MultiBlot SNAP2MB2 1
Components for Western Blotting Procedures
SNAP i.d.® 2.0 MultiBlot Holding Frame SNAP2FRMB01 1
SNAP i.d.® 2.0 Mini Blot Holding Frame (single pack) SNAP2FRMN01 1
SNAP i.d.® 2.0 Mini Blot Holding Frames (double pack) SNAP2FRMN02 1
SNAP i.d.® 2.0 Midi Blot Holding Frame (single pack) SNAP2FRMD01 1
SNAP i.d.® 2.0 Midi Blot Holding Frames (double pack) SNAP2FRMD02 1
SNAP i.d.® 2.0 MultiBlot Holders (includes 2 well blanks) SNAP2BHMB050 50
SNAP i.d.® 2.0 Mini Blot Holders SNAP2BHMN0100 100
SNAP i.d.® 2.0 Midi Blot Holders SNAP2BHMD0100 100
SNAP i.d.® 2.0 Antibody Collection Tray SNAPABTR 20
SNAP i.d.® Blot Roller SNAP2RL 1
Blotting Membranes
Immobilon®-P PVDF, 0.45 μm, 26.5 x 3.75 cm roll IPVH00010 1
Immobilon®-P PVDF, 0.45 μm, 7 x 8.4 cm sheet IPVH07850 50
Immobilon®-P PVDF, 0.45 μm, 8.5 x 13.5 cm sheet IPVH08130 10
Immobilon®-FL PVDF, 0.45 μm, 26.5 x 3.75 cm roll IPFL00010 1
Immobilon®-FL PVDF, 0.45 μm, 7 x 8.4 cm sheet IPFL07810 10
Immobilon®-PSQ PVDF, 0.2 μm, 7 x 8.4 cm sheet ISEQ07850 50
Immobilon®-P PVDF Sandwich, 0.45 μm, 7 x 8.4 cm IPSN07852 20
Immobilon®-P PVDF Sandwich, 0.45 μm, 8.5 x 13.5 cm IPSN08132 20
Reagents for Western Blotting
Luminata™ Forte Western HRP Substrate WBLUF0500 500 mL
Luminata™ Crescendo Western HRP Substrate WBLUR0500 500 mL
Luminata™ Classico Western HRP Substrate WBLUC0500 500 mL
Immobilon® Western HRP Substrate WBKLS0500 500 mL
Calbiochem® SignalBoost™ Immunoreaction Enhancer Kit 407207 1 kit
Re-Blot™ Plus Strong Antibody Stripping Solution, 10X 2504 50 mL
bløk®-CH Buffer WBAVDCH01 500 mL
bløk®-FL Buffer WBAVDFL01 500 mL
bløk®-PO Buffer WBAVDP001 500 mL
Ordering Information
6. 6
Key Benefits
• Eliminates the need for pap pens
• Antibodies can be collected and reused
• Slide handling time is significantly decreased
• Less time spent on wash steps
• Parallel processing of multiple slides
Key Features
• Flexibility of multiple slide configurations enables
the processing of 1 to 24 slides at a time
• Compatible with standard IHC slides and protocols
• Compatible with diverse tissue preparations
including formalin-fixed or fresh frozen samples
• Intuitive format
• Incorporates blocking, washing, and antibody
incubation and labeling steps
• Systematizes handling multiples slides without
the cost of automation
• Test tracker feature on frame cover helps keep track
of IHC steps
SNAP i.d.® 2.0 Protein Detection System for
Immunohistochemistry (IHC) introduces a new
capability to the innovative, vacuum-driven SNAP i.d.®
2.0 system. The IHC frame and slide holders allow you to
block, probe, and stain up to 12 tissue slides per frame.
Reduced handling time and multiple-slide processing
make this system ideal for antibody and protocol
optimization.
How does the SNAP i.d.® 2.0 Protein
Detection System for IHC work?
With two individually controlled sides, the system base
allows for independent, vacuum-driven processing of
either one or two IHC frames. Each of the IHC frames
can process between 1 to 12 glass slides through
independent vacuum ports.
Each slide holder has an injection/recovery port that
enables the manual addition, as well as the removal and
recovery, of small volumes of antibodies or reagents;
reagents can also be flushed using the vacuum feature if
conservation is not a priority.
SNAP i.d.®
2.0 IHC System
Immunohistochemistry
workflow includes blocking,
antibody incubations,
labeling and wash steps, all
of which can be streamlined
using the SNAP i.d.® 2.0
Protein Detection System
for IHC.
SNAP i.d.®
2.0 Steps
Sample Prep
= Wash
Sample Mounting Antigen Recovery (if required) Blocking
Antibody Incubation
Labeling
Staining
Mounting Visualization
7. 7
Comparable performance to
traditional methods, even in
archival tissue
The SNAP i.d.® 2.0 IHC System produces comparable
staining to traditional protocols even in archival tissue.
In the first example below, the system was used to detect
Aquaporin 1 in archival human kidney tissue. Note the
characteristic differential staining of kidney proximal
Detection of Aquaporin
1 in human kidney tissue
(formalin-fixed and
paraffin-embedded (FFPE):
SNAP i.d.® 2.0 IHC System
(sections 1–13) vs. Standard
IHC protocol (section M1).
Fifteen tissue sections (5
μm) were assembled on
FisherBiotech® ProbeOn
Plus™ slides. Slides were
rehydrated and antigen
retrieved (heat-induced
epitope retrieval, HIER) using
Reveal Decloaker (Biocare
Medical, LLC) in a pressure
cooker for 15 minutes at
110 °C. Thirteen slides were
then processed using the
SNAP i.d.® IHC system, and
one was processed using the
manual protocol. Blocking
was performed by incubating
10 min with Punisher™
reagent (Biocare). After
three washes with TBST,
slides were incubated 2 h
with Anti-Aquaporin 1 (Cat.
No. AB2219, 1:2,000). After
three more TBST washes,
slides were incubated 10 min
with Anti-Rabbit Secondary
Antibody (Biocare). Signal
was detected using a HRP-
DAB detection kit (Biocare).
Slides were washed three
times with TBST and
were counterstained with
hematoxylin for 1 min.
After three final washes,
slides were dehydrated with
four 5-minute changes of
100% ethanol, cleared with
three changes of xylene and
coverslipped with Ecomount
medium (Biocare).
tubule epithelium and renal corpuscle. The second
example shows NeuN signal in human brain, localized
as expected to neuronal nuclei. Despite processing 12
slides in parallel and shortening the handling time and
protocol, the staining is robust and consistent with no
blotchy artifacts that sometimes plague autostainers.
There is no apparent tissue degradation as compared
with traditional protocols. Classic histological stains such
as hematoxylin can be applied using the same system.
1 6 11
2 7 12
3 8 13
4 9 M1
5 10
Detection of NeuN in human
cerebral cortex (FFPE): SNAP
i.d.® 2.0 IHC System (left)
vs. standard manual IHC
protocol (right). Anti-NeuN
(Cat. No. MAB377, 1:2,000)
was used to stain sections of
human cerebral cortex using
the protocols described in
the previous figure.
SNAP i.d.® IHC System Manual IHC