Casey Molecular Diagnostic Laboratory has developed a new next-generation sequencing panel method using simplex PCR reactions run on a chip with over 5,000 individual wells, with each primer set duplicated to avoid allele dropout. This new approach offers unmatched target enrichment of over 98% and average coverage over 500x. Every novel variation is manually checked to avoid false positives or negatives. While other labs sequence more broadly, Casey believes their targeted approach focusing on relevant genes optimizes mutation detection.