News, Events, Publications, Videos, Trends, Frequently Asked Questions, Innovation and Technology - All surrounding the field of Primary Cells, used in disease research and drug discovery.

1. Traffic Jam at the Blood–Brain Barrier Promotes Greater Accumulation of
Alzheimer’s Disease Amyloid-β Proteins in the Cerebral Vasculature
Molecular Pharmaceutics, 10:1557–1565Highlight Study
Primary Cell Biology News
Issue 18 Jun 2016
Primary Cells Media & Reagents Cell Biology
Starting Material Matters - Cell signaling pathway strength may vary among donors, so researchers normally
must set aside time to screen cells for sensitivity to specific stimuli. Investigators can save valuable
resources and improve the validity and reproducibility of results through the use of pre-screened cells.
Bovine Brain Microvascular Cells (BBMVEC) were used in this study
Endothelial cells can be pre-screened to demonstrate stimulation-
dependent angiogenesis and key endothelial cell signaling
pathways. Vascular Endothelial Growth Factor Receptor
(VEGFR-2)-mediated signaling plays a critical role in endothelial
cell proliferation, differentiation, movement and survival, as well as
vessel formation. In this pathway, VEGF-induced receptor
dimerization triggers activation of VEGFR-2 tyrosine kinase,
leading to activation of downstream signaling molecules.
Pre-screening helps normalize cell signaling responsiveness to target stimuli
BBMVEC
Human Endothelial Cells:
1. High-Content Screening and Analysis in Cell-Based
Assays for Drug Toxicity and Genotoxicity Testing
Using automated high-res microscopy & analytical software to visualize and
quantify cell responses, HCS determines how compounds affect cell
morphology in a rapid, quantitative, high-throughput manner
2. An Intro To HCS: Imaging Technology, Assay Development
and Data Analysis in Biology & Drug Discovery
A collaborative and interdisciplinary approach covering HCS concepts,
principles, training and tutorials for pharmaceutical industry and academia
3. Assay Development Guidelines for Image-Based High
Content Screening, Analysis and Imaging
Immunofluorescence, confocal and wide-field microscopy with automation,
image analysis and quantitative information to detect multiple changes in single
cells. Used for target ID, compound profiling & development. Primary cell
assays exploit natural genetics, reactions, assembly & physiology.
4. Image-Based High-Content Screening in Drug Discovery
Automated, microscope-based HCS looks at many features
simultaneously in complex biology systems. HCS is used in the
pharmaceutical drug discovery pipeline to validate drug targets &
compounds, predict toxicity, and suggest pathways or molecular targets.
High Content Screening
Browse
Past Issues
21
3 4
Pre-Screened for Angiogenesis & VEGF Signaling
The Figure at right shows activation of VEGFR-2 signaling in
endothelial cells from human aorta (HAOEC), umbilical vein
(HUVEC) and microvessels (HMVEC) by 100 ng/ml VEGF for 0, 5
or 15 minutes. VEGFR-2 autophosphorylation is probed with
phospho-VEGFR-2 specific antibody, while Akt activation is
detected by a phospho-Akt. A phospho-p44/42 MAP kinase
(T202/Y204) antibody is used to demonstrate kinase activation.
The bottom panel illustrates that pre-screened HUVEC & HMVEC
express key endothelial markers Tie2, eNOS, Axl and Etk/Bmx.
Amyloid-β40 (Aβ40) deposition in the brain vasculature results in cerebral amyloid
angiopathy (CAA), which occurs in Alzheimer’s disease. Dutch Mutation of Aβ40
(E22Q) in carriers promotes accumulation in the endothelial cells of the blood–brain-
barrier (BBB). The resulting permeation barrier prevents Amyloid clearance,
promotes deposits in microvessels, and leads to severe CAA. These findings
uncover pathophysiological events that promote CAA and suggest clearing vascular
endothelium of Aβ proteins may halt or even reverse CAA.
1. MicroRNA
suppresses SMC
differentiation involved
in heart disease
Useful Links and Downloads
Dr. Anna Wakeland joins CAI as Director of Technical Sales
Now Trending:
3. Cellular Signaling2. IEEE Transactions Nanotech1. PLoS ONE
3. Downstream
signaling pathways of
common growth
factor described.
Excessive vascular smooth muscle cell proliferation
contributes to pulmonary blockage and hypertension.
MicroRNA suppresses gene signals involved in
differentiation of human pulmonary artery SMCs.
2. Real-time biological
cell sensing system
based on carbon
nanofibers
Non-invasive, label-free measurement of cell
behavior & response: Changes in cell morphology &
integrity provide clues for toxicity, disease diagnosis,
environmental monitoring & drug effectiveness.
Hepatocyte Growth Factor isoform induces cell
motility & survival: HGF Isoform NK2 was studied
in primary Human Bronchial Epithelial Cells
HPASMC
HBEpC
BAOSMC
Primary Cells:
Human PBMCFemoral Artery CellsNeural Stem Cells
MicroRNA, Biological Cell Sensing, Growth Factor Signaling
May 23, 2016 - Cell Applications, Inc. is pleased to announce that Anna Wakeland,
PhD, has joined the company as Director of Technical Sales. In this key role, Dr.
Wakeland will leverage her strong research and presentation skills to grow direct
sales, providing solution-centric, consultative approaches to the customer experience.
Most recently, Anna conducted cell-based research at the University of California,
San Diego, including an interim role as Clinical Research Associate. At UCSD, she
mastered comprehensive human stem cell culture techniques, developed protocols
for differentiation of human pluripotent stem cells and analyzed trophoblast lineage
specification. Prior roles included a research internship at Sandia National
Laboratories. Dr. Wakeland earned her PhD in Biomedical Sciences from UCSD and
completed a Micro-MBA Certificate Program at the Rady School of Management.
She also participated in a Biotechnology Life Science Business Immersion Program
through the BIOCOM Institute. “We are excited about the scientific and professional
skills Dr. Wakeland brings to the commercial life science setting," says Daniel
Schroen, Vice President of Sales and Marketing at CAI. "With her business acumen
and deep knowledge of primary cell biology, Anna will be able to help lab researchers
select optimal products and services to successfully complete even the most intricate,
large scale projects.”
CD34+ Progenitors
Cytofect SMC
Trasnfection Kit
and
Anna Wakeland, PhD
and
Bronchial /
Tracheal Epitheal
Cell Medium
Blood-Brain Barrier
Digestive & Liver Disease, June 2014
PLoS ONE, May 2016
cellapplications.com
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Bovine Brain Microvascular
Endothelial Cells (BBMVEC)
What are integration-
free Human iPSC?
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A Classic System to Study the
Blood-Brain Barrier (BBB)
Integration-free Human Induced Pluripotent Stem
Cells (HiPSC) rely on strategies to enforce the
expression of reprogramming factors in the host
cells that do not imply the insertion of vector DNA
in the host genome. Specifically, the Sendai virus
is an RNA virus that never goes through a DNA
phase, thus there is no risk of random integration
of viral genetic material in the host genome.
The pioneering work describing the generation of
HiPSCs utilized retroviruses to transfer the
reprogramming factors to donor skin fibroblasts.
An effective strategy, the use of retrovirus implies
that the vriuses’ genetic material is integrated in
host genome, along with the transgenes. This
somewhat uncontrolled, random integration could
potentially cause genotoxic stress or affect cell
behavior genes. Thus, integration-free HiPSC can
help ensure an observed phenotype is the result of
a specific genetic mutation or drug.
The Blood-Brain Barrier in 3D
Media Contact
Daniel Schroen, PhD
VP, Sales & Marketing
(858) 453-0848
d.schroen@cellapplications.com
Rat Brain Microvascular
Endothelial Cells (RBMVEC)
Expires June 30, 2016