CellFree Sciences offers a cell-free protein expression platform using wheat germ extracts to rapidly produce proteins without cells. Their technology allows scalable and automated protein production from micrograms to grams, and expression of proteins difficult to produce in cell-based systems. They provide reagents, protein synthesizers, and contract research services to support protein research and production needs.
Agscientific one can find HRV 3C Protease,Cysteine Protease, PreScission Site with the sizes 1mg and 10mg at most affordable prices $242.00 and $1857.60 respectively. HRV 3C Protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.
Tools for improved Protein Mass Spec Sample preparation by PromegaMourad FERHAT, PhD
Proteases, glycosidases, antibody characterization tools, IdeS, IdeZ, mass spec reference protein digests, and new solutions for mass spectrometry analysis. Découvrez nos solutions pour vos analyses en spectrométrie de Masse.
Mourad FERHAT, Ph.D
Chef de Produit Analyse cellulaire
mourad.ferhat@promega.com
Promega France
10 elisa kits for giant cell tumor of bone researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer and bone diseases. It provides the product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect target proteins within given concentration ranges with sensitivities down to the picogram or nanogram per milliliter level. The kits can be used to analyze levels of proteins including TP53, TNF-α, IL-6, CDKN2A, TGF-β1, TNFSF11, MMP-9, PTEN, EGFR, IFN-γ, CTNNβ1, VEGF-A, FGFR3, BMP-2, IGF-1, PDGFRα, and
02 elisa kits for malignant pleural effusion researchElabscience
Malignant pleural effusion is a condition in which cancer causes an
abnormal amount of fluid to collect between the thin layers of tissue
(pleura) lining the outside of the lung and the wall of the chest
cavity. Lung cancer and breast cancer account for about 50-65% of
malignant pleural effusions. Other common causes include pleural
mesothelioma and lymphoma.More info visit: http://www.elabscience.com
BioROIS Co., Ltd is a Japanese venture company established in 2006 to commercialize bio-related technologies developed at the National Institute of Genetics. The company developed the Auxin Inducible Degron (AID) system, which allows for rapid degradation of target proteins in mammalian cells through the addition of auxin. The AID system allows target proteins to be degraded within 30 minutes, much faster than other gene expression control systems. BioROIS licenses the AID system technology and sells AID system kits to support life science research.
The document describes using peptidomics to monitor protease inhibition in vivo by analyzing peptides as surrogates for protease activity. Peptidomics allows the comprehensive analysis of endogenous peptides from biological samples. The study demonstrates inhibiting different proteases in rats, including DPPIV and FXA, and analyzing changes in peptide levels. Several novel protease substrates were identified, including an ITM2B peptide for DPPIV. Collagen peptide levels were also strongly affected by DPPIV inhibition, indicating its importance in collagen metabolism. Peptidomics can thus non-invasively monitor protease activity and inhibition in vivo by analyzing surrogate peptide markers.
Elisa kits for children urinary tract infections researchElabscience
A urinary tract infection (UTI) is an infection that affects part of the urinary tract. When it affects the lower urinary tract it is known as a bladder infection (cystitis) and when it affects the upper urinary tract it is known as kidney infection (pyelonephritis). The most common cause of infection is Escherichia coli, and other bacteria or fungi may rarely be the reason.More info visit: http://www.elabscience.com
09.elisa kits for lower limb pain during pregnancy researchElabscience
Lower limb pain during pregnancy refers to the leg or foot convulsive of pregnant in the late pregnancy. It is mainly caused by inadequacy of blood due to pregnancy. The increasing of uterus in the late pregnancy also affects the blood run of lower limb.More info visit: http://www.elabscience.com
Agscientific one can find HRV 3C Protease,Cysteine Protease, PreScission Site with the sizes 1mg and 10mg at most affordable prices $242.00 and $1857.60 respectively. HRV 3C Protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.
Tools for improved Protein Mass Spec Sample preparation by PromegaMourad FERHAT, PhD
Proteases, glycosidases, antibody characterization tools, IdeS, IdeZ, mass spec reference protein digests, and new solutions for mass spectrometry analysis. Découvrez nos solutions pour vos analyses en spectrométrie de Masse.
Mourad FERHAT, Ph.D
Chef de Produit Analyse cellulaire
mourad.ferhat@promega.com
Promega France
10 elisa kits for giant cell tumor of bone researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer and bone diseases. It provides the product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect target proteins within given concentration ranges with sensitivities down to the picogram or nanogram per milliliter level. The kits can be used to analyze levels of proteins including TP53, TNF-α, IL-6, CDKN2A, TGF-β1, TNFSF11, MMP-9, PTEN, EGFR, IFN-γ, CTNNβ1, VEGF-A, FGFR3, BMP-2, IGF-1, PDGFRα, and
02 elisa kits for malignant pleural effusion researchElabscience
Malignant pleural effusion is a condition in which cancer causes an
abnormal amount of fluid to collect between the thin layers of tissue
(pleura) lining the outside of the lung and the wall of the chest
cavity. Lung cancer and breast cancer account for about 50-65% of
malignant pleural effusions. Other common causes include pleural
mesothelioma and lymphoma.More info visit: http://www.elabscience.com
BioROIS Co., Ltd is a Japanese venture company established in 2006 to commercialize bio-related technologies developed at the National Institute of Genetics. The company developed the Auxin Inducible Degron (AID) system, which allows for rapid degradation of target proteins in mammalian cells through the addition of auxin. The AID system allows target proteins to be degraded within 30 minutes, much faster than other gene expression control systems. BioROIS licenses the AID system technology and sells AID system kits to support life science research.
The document describes using peptidomics to monitor protease inhibition in vivo by analyzing peptides as surrogates for protease activity. Peptidomics allows the comprehensive analysis of endogenous peptides from biological samples. The study demonstrates inhibiting different proteases in rats, including DPPIV and FXA, and analyzing changes in peptide levels. Several novel protease substrates were identified, including an ITM2B peptide for DPPIV. Collagen peptide levels were also strongly affected by DPPIV inhibition, indicating its importance in collagen metabolism. Peptidomics can thus non-invasively monitor protease activity and inhibition in vivo by analyzing surrogate peptide markers.
Elisa kits for children urinary tract infections researchElabscience
A urinary tract infection (UTI) is an infection that affects part of the urinary tract. When it affects the lower urinary tract it is known as a bladder infection (cystitis) and when it affects the upper urinary tract it is known as kidney infection (pyelonephritis). The most common cause of infection is Escherichia coli, and other bacteria or fungi may rarely be the reason.More info visit: http://www.elabscience.com
09.elisa kits for lower limb pain during pregnancy researchElabscience
Lower limb pain during pregnancy refers to the leg or foot convulsive of pregnant in the late pregnancy. It is mainly caused by inadequacy of blood due to pregnancy. The increasing of uterus in the late pregnancy also affects the blood run of lower limb.More info visit: http://www.elabscience.com
The document discusses how AP-2α induces apoptosis in cancer cells. It finds that AP-2α activates the intrinsic apoptosis pathway by binding to the Bcl-2 promoter and repressing its transcription. This allows Bax to translocate to mitochondria, disrupt membrane potential, and release cytochrome c, activating caspase-9 and caspase-3. Downregulation of anti-apoptotic Bcl-2 is important for AP-2α-induced apoptosis. Overexpressing AP-2α enhances cancer cell chemosensitivity to various drugs.
13.elisa kits for aplastic anemia researchElabscience
Aplastic anemia, also spelled aplastic anaemia, is a rare disease in which the bone marrowand the hematopoietic stem cells that reside there are damaged. This causes a deficiency of all three blood cell type: red blood cells , white blood cells,and platelets . Aplastic anemia can be caused by exposure to certain chemicals, drugs, radiation, infection, immune disease; in about half the cases, the cause is unknown.More info visit: http://www.elabscience.com
18 elisa kits for mediastinal tumor researchElabscience
The mediastinum is the cavity that separates the lungs from the rest of the chest. It contains many important organs, such as heart, esophagus, trachea, thymus, aorta, fat, nerve and lymphatic tissue. Mediastinal tumor refers to tumors caused by abnormal developmental or acquired cyst.More info visit: http://www.elabscience.com
Proteintech offers a wide range of markers
and related antibodies for cardiovascular research.
Slideshow includes:
Age-associated Changes in Cardiovascular Tissues
mTOR in Cardiac Physiology and Disease
Cardiac Troponin T Antibody
Cardio-related Antibodies
This document summarizes a study investigating the relationship between activation of the alpha7 nicotinic acetylcholine receptor (α7nAChR) and the nuclear factor erythroid 2–related factor 2 (Nrf2) in antagonizing renal ischemia-reperfusion injury. The study examined the effects of a selective α7nAChR agonist on apoptosis and apoptotic signaling in renal epithelial cells subjected to hypoxia-reoxygenation injury. It was found that the agonist attenuated apoptosis and modulated levels of apoptotic proteins like caspase-3 and Bax. Inhibition of Nrf2 partially blocked these effects, indicating Nrf2 mediates the anti-apoptotic effects of α7nAChR activation
Elisa kits for congenital heart disease researchElabscience
Congenital heart defect (CHD), also known as a congenital heart anomaly or congenital heart
disease, is a problem in the structure of the heart that is present at birth.Signs and
symptoms depend on the specific type of problem. Symptoms can vary from none to life-
threatening.The cause of a congenital heart defect is often unknown.Most congenital heart
problems do not occur with other diseases. Complications that can result from heart defects
include heart failure.More info visit: http://www.elabscience.com
ELISA Kits for Signal Transduction ResearchElabscience
Signal transduction plays an important role in activating cellular functions, cell growth, cell differentiation and cell proliferation. Elabscience provides hundreds of ELISA Kits coated with signal transduction targets for you to chose, which may help you in the related research fields. More info visit: http://www.elabscience.com
37 elisa kits for renal carcinoma researchElabscience
Renal carcinoma (RC) is a kidney cancer that originates in the lining of the proximal convoluted tubule, a part of the very small tubes in the kidney that transport waste molecules from the blood to the urine. Symptoms include haematuria, flank pain , abnormal mass, malaise, anemia, erythrocytosis, varicocele and hypertension, etc.More info visit: http://www.elabscience.com
01 elisa kits for malignant ascites researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer. For each kit, it provides the product code, protein name being detected, detection range, sensitivity, and test method. The kits use ELISA (enzyme-linked immunosorbent assay) to detect proteins including TP53, CDKN2A, KRAS, EGFR, CCND1, VEGF-A, IL-6, PDGFRβ, ALB, BRAF, JAK2, ERα, MMP-2, BRCA2, TM, MMP-9, and BRCA1 within certain concentration ranges with good sensitivity. All kits employ a sandwich ELISA test method.
April 2014 - Antibodies Newsflash - BBI SolutionsBBISolutions
This month’s newsflash focuses on our recombinant Fab antibodies. Find out more about their clinical relevance and see new performance data for Cystatin C, Myeloperoxidase and C Reactive Protein recombinant Fab antibodies.
Oral cancer, also known as mouth cancer, is a type of head and neck
cancer and is any cancerous tissue growth located in the oral cavity.
Oral or mouth cancer most commonly involves the tongue. It may also
occur on the floor of the mouth, cheek lining, gingiva (gums), lips, or
palate (roof of the mouth). Oncogenes are activated as a result of
mutation of the DNA. Risk factors that predispose a person to oral
cancer have been identified in epidemiological (epidemiology)
studies.More info visit: http://www.elabscience.com
Elisa kits for upper respiratory tract infection researchElabscience
Upper respiratory tract infections (URI or URTI) are illnesses caused by an acute infection which involves the upper respiratory tract including the nose, sinuses, pharynx or larynx. This commonly includes tonsillitis, pharyngitis, laryngitis, sinusitis, otitis media, and the common cold. More info visit: http://www.elabscience.com
Clinical Samples & Disease State Plasma Newsflash October 2016BBISolutions
This document provides information on the inventory and capabilities of BBI Biobank, a company that collects and sells clinical samples and disease state plasma. It lists various plasma and serum products available for diseases like infectious diseases (HIV, HCV, HBV), autoimmune/non-infectious diseases (anti-GBM, CRP), and depleted serum products. It also describes the company's custom sample collection and depletion services. Key capabilities highlighted include direct collection from blood banks and hospitals, comprehensive characterization of samples, and operating under ISO standards.
Elisa kits for infectious mononucleosis researchElabscience
The document lists various ELISA kits for detecting human proteins and their specifications. It provides the product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect the target protein within a given concentration range, with sensitivities ranging from below 1 ng/mL to 8,000 pg/mL. The kits can be used to detect proteins involved in processes like inflammation, cell signaling, neurobiology, and extracellular matrix remodeling.
19 elisa kits for spinal tumor researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in oncology. It provides the product code, product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect proteins within given concentration ranges, with sensitivities down to the picogram or nanogram per milliliter level. The kits can be used to detect levels of tumor-related proteins including p53, TNF-alpha, PTEN, CDKN2A, CTNNB1, BDNF, GDNF, EGFR, AR, IL-6, PDGFR-alpha, VEGF-A, IGF-2, TNFRSF1A, TNFRSF1B, KR
11 elisa kits for trophoblastic tumor malignant researchElabscience
Tumors differentiated from embryonic trophoblast cells are called
trophoblastic tumor, such as hydatidiform mole, invasive hydatidiform
mole and choriocarcinoma, etc. Invasive hydatidiform mole and
choriocarcinoma are malignant trophoblastic tumor. Common symptoms
include vaginal bleeding, hemoptysis, abdominal pain and abdominal
mass.More info visit: http://www.elabscience.com
04..elisa kits for peyronie disease researchElabscience
Prostatitis is inflammation of the prostate gland. Prostatitis is classified into acute, chronic, asymptomatic inflammatory prostatitis, and chronic pelvic pain syndrome. Acute prostatitis is a bacterial infection of the prostate gland that requires urgent medical treatment. Chronic prostatitis is a pelvic paincondition in men, formerly known as prostatodynia (painful prostate).More info visit: http://www.elabscience.com
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
A bone tumor, (also spelled bone tumour), is a neoplastic growth of tissue in bone. The most common symptom of bone tumors is pain, which will gradually increase over time. Additional symptoms may include fatigue, fever, weight loss, anemia, and/or unexplained bone fractures. More info visit: http://www.elabscience.com
48 elisa kits for thyroid adenoma researchElabscience
A thyroid adenoma is a benign tumor of the thyroid gland. A thyroid adenoma may be clinically silent ("cold" or "warm" adenoma), or it may be a functional tumor, producing excessive thyroid hormone ("hot" adenoma). In this case, it may result in symptomatic hyperthyroidism, and may be referred to as a toxic thyroid adenoma.More info visit: http://www.elabscience.com
This document discusses in silico proteolysis of food proteins. It begins by defining in silico, in vitro, and in vivo testing. In silico proteolysis is performed using computer simulation to analyze protein digestion. Several online databases and tools for in silico peptide digestion and prediction are described, including BIOPEP, UniProt, MEROPS, PROSPER, BLAST, and ExPASy tools like PeptideCutter and PeptideMass. Previous studies have identified bioactive peptides with effects like ACE inhibition from proteins in meats, fish, and plant sources. In silico methods allow large-scale, low-cost simulation of protein digestion to discover bioactive peptides for developing functional foods.
Protein Thermal Shift™ Solution Using Applied Biosystems Real-Time PCR SystemsThermo Fisher Scientific
Life Technologies Product Manager for Real-time PCR Systems, Levente Egry, provides an overview of Protein Thermal Shift™ technology, products and applications at the PepTalk 2012 in San Diego, California.
To learn more about the solutions in this presentation, visit: www.appliedbiosystems.com/proteinmelt
The document discusses how AP-2α induces apoptosis in cancer cells. It finds that AP-2α activates the intrinsic apoptosis pathway by binding to the Bcl-2 promoter and repressing its transcription. This allows Bax to translocate to mitochondria, disrupt membrane potential, and release cytochrome c, activating caspase-9 and caspase-3. Downregulation of anti-apoptotic Bcl-2 is important for AP-2α-induced apoptosis. Overexpressing AP-2α enhances cancer cell chemosensitivity to various drugs.
13.elisa kits for aplastic anemia researchElabscience
Aplastic anemia, also spelled aplastic anaemia, is a rare disease in which the bone marrowand the hematopoietic stem cells that reside there are damaged. This causes a deficiency of all three blood cell type: red blood cells , white blood cells,and platelets . Aplastic anemia can be caused by exposure to certain chemicals, drugs, radiation, infection, immune disease; in about half the cases, the cause is unknown.More info visit: http://www.elabscience.com
18 elisa kits for mediastinal tumor researchElabscience
The mediastinum is the cavity that separates the lungs from the rest of the chest. It contains many important organs, such as heart, esophagus, trachea, thymus, aorta, fat, nerve and lymphatic tissue. Mediastinal tumor refers to tumors caused by abnormal developmental or acquired cyst.More info visit: http://www.elabscience.com
Proteintech offers a wide range of markers
and related antibodies for cardiovascular research.
Slideshow includes:
Age-associated Changes in Cardiovascular Tissues
mTOR in Cardiac Physiology and Disease
Cardiac Troponin T Antibody
Cardio-related Antibodies
This document summarizes a study investigating the relationship between activation of the alpha7 nicotinic acetylcholine receptor (α7nAChR) and the nuclear factor erythroid 2–related factor 2 (Nrf2) in antagonizing renal ischemia-reperfusion injury. The study examined the effects of a selective α7nAChR agonist on apoptosis and apoptotic signaling in renal epithelial cells subjected to hypoxia-reoxygenation injury. It was found that the agonist attenuated apoptosis and modulated levels of apoptotic proteins like caspase-3 and Bax. Inhibition of Nrf2 partially blocked these effects, indicating Nrf2 mediates the anti-apoptotic effects of α7nAChR activation
Elisa kits for congenital heart disease researchElabscience
Congenital heart defect (CHD), also known as a congenital heart anomaly or congenital heart
disease, is a problem in the structure of the heart that is present at birth.Signs and
symptoms depend on the specific type of problem. Symptoms can vary from none to life-
threatening.The cause of a congenital heart defect is often unknown.Most congenital heart
problems do not occur with other diseases. Complications that can result from heart defects
include heart failure.More info visit: http://www.elabscience.com
ELISA Kits for Signal Transduction ResearchElabscience
Signal transduction plays an important role in activating cellular functions, cell growth, cell differentiation and cell proliferation. Elabscience provides hundreds of ELISA Kits coated with signal transduction targets for you to chose, which may help you in the related research fields. More info visit: http://www.elabscience.com
37 elisa kits for renal carcinoma researchElabscience
Renal carcinoma (RC) is a kidney cancer that originates in the lining of the proximal convoluted tubule, a part of the very small tubes in the kidney that transport waste molecules from the blood to the urine. Symptoms include haematuria, flank pain , abnormal mass, malaise, anemia, erythrocytosis, varicocele and hypertension, etc.More info visit: http://www.elabscience.com
01 elisa kits for malignant ascites researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in cancer. For each kit, it provides the product code, protein name being detected, detection range, sensitivity, and test method. The kits use ELISA (enzyme-linked immunosorbent assay) to detect proteins including TP53, CDKN2A, KRAS, EGFR, CCND1, VEGF-A, IL-6, PDGFRβ, ALB, BRAF, JAK2, ERα, MMP-2, BRCA2, TM, MMP-9, and BRCA1 within certain concentration ranges with good sensitivity. All kits employ a sandwich ELISA test method.
April 2014 - Antibodies Newsflash - BBI SolutionsBBISolutions
This month’s newsflash focuses on our recombinant Fab antibodies. Find out more about their clinical relevance and see new performance data for Cystatin C, Myeloperoxidase and C Reactive Protein recombinant Fab antibodies.
Oral cancer, also known as mouth cancer, is a type of head and neck
cancer and is any cancerous tissue growth located in the oral cavity.
Oral or mouth cancer most commonly involves the tongue. It may also
occur on the floor of the mouth, cheek lining, gingiva (gums), lips, or
palate (roof of the mouth). Oncogenes are activated as a result of
mutation of the DNA. Risk factors that predispose a person to oral
cancer have been identified in epidemiological (epidemiology)
studies.More info visit: http://www.elabscience.com
Elisa kits for upper respiratory tract infection researchElabscience
Upper respiratory tract infections (URI or URTI) are illnesses caused by an acute infection which involves the upper respiratory tract including the nose, sinuses, pharynx or larynx. This commonly includes tonsillitis, pharyngitis, laryngitis, sinusitis, otitis media, and the common cold. More info visit: http://www.elabscience.com
Clinical Samples & Disease State Plasma Newsflash October 2016BBISolutions
This document provides information on the inventory and capabilities of BBI Biobank, a company that collects and sells clinical samples and disease state plasma. It lists various plasma and serum products available for diseases like infectious diseases (HIV, HCV, HBV), autoimmune/non-infectious diseases (anti-GBM, CRP), and depleted serum products. It also describes the company's custom sample collection and depletion services. Key capabilities highlighted include direct collection from blood banks and hospitals, comprehensive characterization of samples, and operating under ISO standards.
Elisa kits for infectious mononucleosis researchElabscience
The document lists various ELISA kits for detecting human proteins and their specifications. It provides the product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect the target protein within a given concentration range, with sensitivities ranging from below 1 ng/mL to 8,000 pg/mL. The kits can be used to detect proteins involved in processes like inflammation, cell signaling, neurobiology, and extracellular matrix remodeling.
19 elisa kits for spinal tumor researchElabscience
The document lists various ELISA kits for detecting levels of proteins involved in oncology. It provides the product code, product name, detection range, sensitivity, and test method for each kit. All kits use a sandwich ELISA method to detect proteins within given concentration ranges, with sensitivities down to the picogram or nanogram per milliliter level. The kits can be used to detect levels of tumor-related proteins including p53, TNF-alpha, PTEN, CDKN2A, CTNNB1, BDNF, GDNF, EGFR, AR, IL-6, PDGFR-alpha, VEGF-A, IGF-2, TNFRSF1A, TNFRSF1B, KR
11 elisa kits for trophoblastic tumor malignant researchElabscience
Tumors differentiated from embryonic trophoblast cells are called
trophoblastic tumor, such as hydatidiform mole, invasive hydatidiform
mole and choriocarcinoma, etc. Invasive hydatidiform mole and
choriocarcinoma are malignant trophoblastic tumor. Common symptoms
include vaginal bleeding, hemoptysis, abdominal pain and abdominal
mass.More info visit: http://www.elabscience.com
04..elisa kits for peyronie disease researchElabscience
Prostatitis is inflammation of the prostate gland. Prostatitis is classified into acute, chronic, asymptomatic inflammatory prostatitis, and chronic pelvic pain syndrome. Acute prostatitis is a bacterial infection of the prostate gland that requires urgent medical treatment. Chronic prostatitis is a pelvic paincondition in men, formerly known as prostatodynia (painful prostate).More info visit: http://www.elabscience.com
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
A bone tumor, (also spelled bone tumour), is a neoplastic growth of tissue in bone. The most common symptom of bone tumors is pain, which will gradually increase over time. Additional symptoms may include fatigue, fever, weight loss, anemia, and/or unexplained bone fractures. More info visit: http://www.elabscience.com
48 elisa kits for thyroid adenoma researchElabscience
A thyroid adenoma is a benign tumor of the thyroid gland. A thyroid adenoma may be clinically silent ("cold" or "warm" adenoma), or it may be a functional tumor, producing excessive thyroid hormone ("hot" adenoma). In this case, it may result in symptomatic hyperthyroidism, and may be referred to as a toxic thyroid adenoma.More info visit: http://www.elabscience.com
This document discusses in silico proteolysis of food proteins. It begins by defining in silico, in vitro, and in vivo testing. In silico proteolysis is performed using computer simulation to analyze protein digestion. Several online databases and tools for in silico peptide digestion and prediction are described, including BIOPEP, UniProt, MEROPS, PROSPER, BLAST, and ExPASy tools like PeptideCutter and PeptideMass. Previous studies have identified bioactive peptides with effects like ACE inhibition from proteins in meats, fish, and plant sources. In silico methods allow large-scale, low-cost simulation of protein digestion to discover bioactive peptides for developing functional foods.
Protein Thermal Shift™ Solution Using Applied Biosystems Real-Time PCR SystemsThermo Fisher Scientific
Life Technologies Product Manager for Real-time PCR Systems, Levente Egry, provides an overview of Protein Thermal Shift™ technology, products and applications at the PepTalk 2012 in San Diego, California.
To learn more about the solutions in this presentation, visit: www.appliedbiosystems.com/proteinmelt
Recombinant protein expression and purification Lecturetest
The document discusses recombinant protein expression and engineering. It describes:
1) Cloning or synthesizing the gene of interest, making an expression construct, transfecting cells, purifying the recombinant protein.
2) Factors to consider like the protein's origin (prokaryotic/eukaryotic), required post-translational modifications, and available expression systems.
3) A case study expressing recombinant human alpha-1-acid glycoprotein in E. coli, including vector construction, periplasmic extraction, affinity purification, and yield.
Improved Reagents & Methods for Target Enrichment in Next Generation Sequencing, presented by Dr Mark Behlke, Chief Scientific Officer at Integrated DNA Technologies
The Role of BPOG Extractables Data in the Effective Adoption of Single-Use Sy...Merck Life Sciences
The successful adoption of single-use technologies in a biopharmaceutical process largely relies on confidently selecting the right components for use in the fluid path of a product, within a specific process. An important step in choosing such components requires generating an extractables profile, which can be done by carefully selecting the solvent streams and extraction conditions to model the product and process steps complemented with the right analytical strategy.
In this webinar, you will learn:
● An approach to adopt the BioPhorum Operations Group (BPOG) extractables protocol as a baseline testing strategy.
● How to apply extractables data to a specific process followed by a systematic, risk-based safety assessment approach used for comparing known safety concern thresholds.
● The important stages in the risk assessment process as demonstrated by case studies from typical drug manufacturing processes where single-use components were used.
This document describes Protea's laboratory and mass spectrometry capabilities for proteomics sample analysis. It offers a variety of products for sample preparation prior to mass spectrometry, including tools for gel-based and solution-based proteomics workflows. Products cover sample isolation, fractionation, digestion, separation, and analysis steps. Services include protein identification and characterization using the company's mass spectrometers.
In this webinar, Edward LeCluyse, PhD, presents a novel, all-human hepatic triculture system and discusses how it addresses the need for a convenient and human-relevant long-term hepatic culture.
The use of primary human hepatocytes in preclinical pharmacological and toxicological applications has been restricted in part by the lack of a suitable culture platform that is convenient, supports a multitude of donor lots, and maintains structural and functional properties of the cells over prolonged periods of time. The sandwich system and previous co-cultures have addressed some of these issues; however, some limitations remain including the short- to mid-term loss of cell polarity and metabolic functions. Animal models are an option, but there are marked species differences in hepatic metabolic and receptor-signaling pathways. The new triculture system from LifeNet Health LifeSciences was developed to offer researchers a convenient all-human hepatic system that maintains both hepatocellular morphology and metabolic function over several weeks.
This new system offers a superior combination of human relevance, reproducibility, longevity, and convenience. It contains 100% all-human cryopreserved hepatocytes and feeder cells with optimized media. Hepatocytes are pre-qualified in the system to meet minimum specifications for morphology, architecture, and basic hepatic functionality for at least two weeks. The system gives researchers control over experimental timelines and workflow in a standard plate format. It can be set up in a matter of hours and tested in multiple applications. These appealing features make this new triculture a suitable model for hepatocyte and liver studies – including metabolic clearance, drug-induced liver injury, risk assessment, disease modeling, and high content imaging – in addition to distinguishing it significantly from current alternative hepatocyte culture systems.
Key Topics Include:
- The LifeNet Health LifeSciences all-human hepatic triculture model accurately and consistently mimics native liver tissue architecture and key hepatocellular functions over several weeks.
- Hepatocytes are pre-qualified to reduce variability and enable a broad range of pharmacological and toxicological applications.
- This hepatic system gives researchers the flexibility to choose from a selection of hepatocyte lots that enables a broad population diversity.
- The system is convenient, offering the simplicity of a 2D model, along with the robust levels of data that typically accompany 3D models.
This white paper describes SABiosciences' Oligo GEArray DNA microarray system. Key points:
1) Oligo GEArrays profile gene expression of focused gene panels (113-440 genes) related to biological pathways or disease states, providing a more economical alternative to genome-wide microarrays.
2) The system uses flexible target labeling kits, membrane arrays, and a simple chemiluminescent detection method, allowing it to be easily adopted in any laboratory.
3) Data shows the Oligo GEArray has high sensitivity (can detect 10 fM targets), a wide dynamic range, and is highly reproducible, with technical replicates yielding CVs less than 10%.
The document summarizes Biotest's blood bank product offerings, including their traditional blood bank reagents and the Tango automated blood bank analyzer. It provides an overview of Biotest's history in transfusion diagnostics and leadership. Evaluation studies showed Biotest's rare antisera outperformed competitors' products in many cases. The Tango system offers flexibility, ease of use, and on-board reagent storage for up to seven days.
The document describes STRATEC Molecular's portfolio of nucleic acid purification kits and sample preparation solutions. It highlights the InviGenius automated nucleic acid purification system, which can process 12 samples directly from primary tubes to PCR-ready DNA/RNA. It also discusses the PSP SalivaGene DNA Kit for non-invasive saliva collection and DNA stabilization at room temperature for 12 months, and the PSP Spin Stool DNA Plus Kit for integrated stool sample collection, transport, and DNA purification.
Target enrichment enables researchers to focus their next generation sequencing (NGS) efforts on regions of interest, allowing them to obtain more sequencing data relevant to their study. In-solution target capture is a method of enrichment using oligonucleotide probes directed to specific regions within a genome. Target capture can be used to enrich multiple samples simultaneously, reducing the cost per sample, while using individually synthesized probes allows researchers to construct gene panels that can be optimized over time.
Long Acting Injectables - A New Dimension for Proteins and PeptidesMilliporeSigma
This webinar discusses long-acting injectable microparticle formulations using SynBiosys® technology for sustained release of proteins and peptides. Case studies are presented on the sustained release of exenatide, a sensitive peptide, and a monoclonal antibody from biodegradable microparticles. Both proteins showed intact structure and biological activity for over a month in vitro and in animal studies, demonstrating the potential of this platform for long-term drug delivery of biologics.
Long Acting Injectables - A New Dimension for Proteins and PeptidesMerck Life Sciences
Access the recording: https://bit.ly/2xAaMba
Abstract:
Long acting injectables (LAI) have been around for decades for the delivery of small molecules and peptides to treat chronic and site-specific diseases. However, when it comes to more sensitive biological therapeutics the classical polylactide and polylactide/glycolide based systems suffer from several limitations (e.g. uncontrolled release kinetics, in situ pH drop, protein degradation) making them unsuitable. The SynBiosys® biodegradable polymeric microparticle technology combines all the features required for LAI formulations for biologics. In two case studies we will showcase sustained release formulations for peptides and proteins and demonstrate their potential via extensive in vitro and in vivo characterization.
This document provides a summary of Solis BioDyne's product catalogue for 2015. It highlights the company's 20 years in business providing quality PCR and qPCR reagents. All of Solis BioDyne's products are stable at ambient temperature due to a proprietary modification called Stability TAG. This allows reagents to be shipped and stored without ice or refrigeration. The document provides specifications for the company's qPCR mixes and lists their compatibility with major real-time PCR platforms. It also summarizes the quality control testing done on products to ensure high performance.
Gel Company provides products with superior performance that are simple, safe and environmentally friendly. Gel Company offers accessories for the following Applied Biosystems products.
GenoThreat / GenoGUARD -- open source biosecurity solution for the gene synth...madalladam
DNA sequence screening software that implements the best match method recommended by the federal government.
Publication: Adam L et al, Strengths and limitations of the federal guidance on synthetic DNA, Nature Biotechnology (2011) 29, 208–210 doi:10.1038/nbt.1802
US Department of Health and Human Services voluntary guidelines “Screening Framework Guidance for Synthetic Double-Stranded DNA Providers” November 2009.
Software: http://sourceforge.net/projects/genothreat/
Antibiotic-detection and Antimicrobial residuesManeSachin1
This document discusses monitoring veterinary drug residues in milk and milk products in India. It summarizes several testing methods available, including microbial inhibitor tests, enzyme inhibition tests, and immune receptor tests. Microbial inhibitor tests are reliable and cost-effective, but require several hours for incubation. The document also discusses developing a novel test using bacterial spores as biosensors that can detect residues in under 3 hours and has been patented. Commercial tests based on enzyme inhibition and immune receptor principles are also briefly described.
Hydrolysate Characterization Technical Presentation Webinar11 2009Mason Williams
1) The document discusses developing a chemically defined hydrolysate alternative to mitigate risks associated with undefined natural hydrolysates.
2) The approach involves fractionating multiple hydrolysates using RP-HPLC and identifying bioactive fractions that stimulate cell growth and protein production in CHO cells.
3) A new product called EX-CELL CD Hydrolysate Fusion was developed that contains synthetic components identified from bioactive fractions and provides 80-100% of the performance of natural hydrolysates.
Why You Should Replace Windows 11 with Nitrux Linux 3.5.0 for enhanced perfor...SOFTTECHHUB
The choice of an operating system plays a pivotal role in shaping our computing experience. For decades, Microsoft's Windows has dominated the market, offering a familiar and widely adopted platform for personal and professional use. However, as technological advancements continue to push the boundaries of innovation, alternative operating systems have emerged, challenging the status quo and offering users a fresh perspective on computing.
One such alternative that has garnered significant attention and acclaim is Nitrux Linux 3.5.0, a sleek, powerful, and user-friendly Linux distribution that promises to redefine the way we interact with our devices. With its focus on performance, security, and customization, Nitrux Linux presents a compelling case for those seeking to break free from the constraints of proprietary software and embrace the freedom and flexibility of open-source computing.
Maruthi Prithivirajan, Head of ASEAN & IN Solution Architecture, Neo4j
Get an inside look at the latest Neo4j innovations that enable relationship-driven intelligence at scale. Learn more about the newest cloud integrations and product enhancements that make Neo4j an essential choice for developers building apps with interconnected data and generative AI.
Alt. GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using ...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Enchancing adoption of Open Source Libraries. A case study on Albumentations.AIVladimir Iglovikov, Ph.D.
Presented by Vladimir Iglovikov:
- https://www.linkedin.com/in/iglovikov/
- https://x.com/viglovikov
- https://www.instagram.com/ternaus/
This presentation delves into the journey of Albumentations.ai, a highly successful open-source library for data augmentation.
Created out of a necessity for superior performance in Kaggle competitions, Albumentations has grown to become a widely used tool among data scientists and machine learning practitioners.
This case study covers various aspects, including:
People: The contributors and community that have supported Albumentations.
Metrics: The success indicators such as downloads, daily active users, GitHub stars, and financial contributions.
Challenges: The hurdles in monetizing open-source projects and measuring user engagement.
Development Practices: Best practices for creating, maintaining, and scaling open-source libraries, including code hygiene, CI/CD, and fast iteration.
Community Building: Strategies for making adoption easy, iterating quickly, and fostering a vibrant, engaged community.
Marketing: Both online and offline marketing tactics, focusing on real, impactful interactions and collaborations.
Mental Health: Maintaining balance and not feeling pressured by user demands.
Key insights include the importance of automation, making the adoption process seamless, and leveraging offline interactions for marketing. The presentation also emphasizes the need for continuous small improvements and building a friendly, inclusive community that contributes to the project's growth.
Vladimir Iglovikov brings his extensive experience as a Kaggle Grandmaster, ex-Staff ML Engineer at Lyft, sharing valuable lessons and practical advice for anyone looking to enhance the adoption of their open-source projects.
Explore more about Albumentations and join the community at:
GitHub: https://github.com/albumentations-team/albumentations
Website: https://albumentations.ai/
LinkedIn: https://www.linkedin.com/company/100504475
Twitter: https://x.com/albumentations
GraphSummit Singapore | The Art of the Possible with Graph - Q2 2024Neo4j
Neha Bajwa, Vice President of Product Marketing, Neo4j
Join us as we explore breakthrough innovations enabled by interconnected data and AI. Discover firsthand how organizations use relationships in data to uncover contextual insights and solve our most pressing challenges – from optimizing supply chains, detecting fraud, and improving customer experiences to accelerating drug discoveries.
Goodbye Windows 11: Make Way for Nitrux Linux 3.5.0!SOFTTECHHUB
As the digital landscape continually evolves, operating systems play a critical role in shaping user experiences and productivity. The launch of Nitrux Linux 3.5.0 marks a significant milestone, offering a robust alternative to traditional systems such as Windows 11. This article delves into the essence of Nitrux Linux 3.5.0, exploring its unique features, advantages, and how it stands as a compelling choice for both casual users and tech enthusiasts.
Generative AI Deep Dive: Advancing from Proof of Concept to ProductionAggregage
Join Maher Hanafi, VP of Engineering at Betterworks, in this new session where he'll share a practical framework to transform Gen AI prototypes into impactful products! He'll delve into the complexities of data collection and management, model selection and optimization, and ensuring security, scalability, and responsible use.
Pushing the limits of ePRTC: 100ns holdover for 100 daysAdtran
At WSTS 2024, Alon Stern explored the topic of parametric holdover and explained how recent research findings can be implemented in real-world PNT networks to achieve 100 nanoseconds of accuracy for up to 100 days.
Removing Uninteresting Bytes in Software FuzzingAftab Hussain
Imagine a world where software fuzzing, the process of mutating bytes in test seeds to uncover hidden and erroneous program behaviors, becomes faster and more effective. A lot depends on the initial seeds, which can significantly dictate the trajectory of a fuzzing campaign, particularly in terms of how long it takes to uncover interesting behaviour in your code. We introduce DIAR, a technique designed to speedup fuzzing campaigns by pinpointing and eliminating those uninteresting bytes in the seeds. Picture this: instead of wasting valuable resources on meaningless mutations in large, bloated seeds, DIAR removes the unnecessary bytes, streamlining the entire process.
In this work, we equipped AFL, a popular fuzzer, with DIAR and examined two critical Linux libraries -- Libxml's xmllint, a tool for parsing xml documents, and Binutil's readelf, an essential debugging and security analysis command-line tool used to display detailed information about ELF (Executable and Linkable Format). Our preliminary results show that AFL+DIAR does not only discover new paths more quickly but also achieves higher coverage overall. This work thus showcases how starting with lean and optimized seeds can lead to faster, more comprehensive fuzzing campaigns -- and DIAR helps you find such seeds.
- These are slides of the talk given at IEEE International Conference on Software Testing Verification and Validation Workshop, ICSTW 2022.
GridMate - End to end testing is a critical piece to ensure quality and avoid...ThomasParaiso2
End to end testing is a critical piece to ensure quality and avoid regressions. In this session, we share our journey building an E2E testing pipeline for GridMate components (LWC and Aura) using Cypress, JSForce, FakerJS…
zkStudyClub - Reef: Fast Succinct Non-Interactive Zero-Knowledge Regex ProofsAlex Pruden
This paper presents Reef, a system for generating publicly verifiable succinct non-interactive zero-knowledge proofs that a committed document matches or does not match a regular expression. We describe applications such as proving the strength of passwords, the provenance of email despite redactions, the validity of oblivious DNS queries, and the existence of mutations in DNA. Reef supports the Perl Compatible Regular Expression syntax, including wildcards, alternation, ranges, capture groups, Kleene star, negations, and lookarounds. Reef introduces a new type of automata, Skipping Alternating Finite Automata (SAFA), that skips irrelevant parts of a document when producing proofs without undermining soundness, and instantiates SAFA with a lookup argument. Our experimental evaluation confirms that Reef can generate proofs for documents with 32M characters; the proofs are small and cheap to verify (under a second).
Paper: https://eprint.iacr.org/2023/1886
UiPath Test Automation using UiPath Test Suite series, part 5DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 5. In this session, we will cover CI/CD with devops.
Topics covered:
CI/CD with in UiPath
End-to-end overview of CI/CD pipeline with Azure devops
Speaker:
Lyndsey Byblow, Test Suite Sales Engineer @ UiPath, Inc.
UiPath Test Automation using UiPath Test Suite series, part 6DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 6. In this session, we will cover Test Automation with generative AI and Open AI.
UiPath Test Automation with generative AI and Open AI webinar offers an in-depth exploration of leveraging cutting-edge technologies for test automation within the UiPath platform. Attendees will delve into the integration of generative AI, a test automation solution, with Open AI advanced natural language processing capabilities.
Throughout the session, participants will discover how this synergy empowers testers to automate repetitive tasks, enhance testing accuracy, and expedite the software testing life cycle. Topics covered include the seamless integration process, practical use cases, and the benefits of harnessing AI-driven automation for UiPath testing initiatives. By attending this webinar, testers, and automation professionals can gain valuable insights into harnessing the power of AI to optimize their test automation workflows within the UiPath ecosystem, ultimately driving efficiency and quality in software development processes.
What will you get from this session?
1. Insights into integrating generative AI.
2. Understanding how this integration enhances test automation within the UiPath platform
3. Practical demonstrations
4. Exploration of real-world use cases illustrating the benefits of AI-driven test automation for UiPath
Topics covered:
What is generative AI
Test Automation with generative AI and Open AI.
UiPath integration with generative AI
Speaker:
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
UiPath Test Automation using UiPath Test Suite series, part 6
Accelerating Protein Research
1. 7/18/2013 Matthias Harbers 1
Dr. Matthias Harbers – Vice President Sales and Marketing Division
Accelerating Protein Research
CellFree Sciences
Reagents – Protein Synthesizers – Protein Expression Services
CellFree Sciences 7/2013
2. 7/18/2013 Matthias Harbers 2
CellFree Sciences offers a cell-free protein expression platform
technology to rapidly deliver proteins for a broad range of
applications:
- ENDEXT® Technology -
What We Can Offer
Our Platform Technology Is Accelerating Protein Research!
3. 7/18/2013 Matthias Harbers 3
The Company
CellFree Sciences Co., Ltd.
Based on ENDEXT® Technology
developed in the laboratory of
Prof. Y. Endo
Protein expression reagents
Protein synthesizers
Protein expression services
Contract research
Technology licensing
Yokohama Business Headquarters
Matsuyama Headquarters
( Ehime University)
Established July 2002 at Ehime University, Japan
4. 7/18/2013 Matthias Harbers 4
Cell-free protein synthesis is the preferable protein preparation method for:
• Rapid protein production directly from DNA template
• Scalable protein production and automation (g to g amounts)
• Expression of difficult to express proteins (membrane proteins, toxic proteins)
• Studies on protein-protein, protein-DNA, or protein-RNA interactions
• Functional analysis of mutations, truncations, protein domains
• Protein engineering and use of artificial amino acids
• Protein labeling with stable isotopes (MS, NMR, crystallography)
• Production of antigens
~40% of all scientists in life science work on or otherwise use proteins for their studies!
Why Cell-Free Protein Synthesis?
5. 7/18/2013 Matthias Harbers 5
Our Technology
CellFree Sciences holds IP on special production methods for high activity
wheat germ extracts removing inhibiting activities from the seed:
Before washing steps After washing steps
0
10
20
30
40
50
60
70
80
90
100
0 2 4 6 8 10 12 14
YieldGFPinmg/ml
Time in days
(Data provided by Prof. Y. Endo)
“washed”
“unwashed”
8-10mg/ml
GFP
6. 7/18/2013 Matthias Harbers 6
Committed to Continuous Innovation
Publication Ehime University
(new wheat germ extract preparation)2000
CFS releases WEPRO1240H/G
(Optimized for His- and GTS-fusion proteins)2005
CFS releases WEPRO1240
(Standard wheat germ extract)2004
CFS releases WEPRO7240 Series
(High performance wheat germ extracts)2009
CFS releases WEPRO9240 Series
(All-in-one ready setup reagents)2013
7. 7/18/2013 Matthias Harbers 7
System Tag High Yield Low Yield Failed No Data
CFS Wheat germ (in vitro) 5’ GST 48 2 - -
CFS Wheat germ (in vitro) 3’ His 41 5 4 -
E. coli (in vitro) 5’ GST 28 2 20 -
E. coli (in vitro) 3’ His 6 - 42 2
E. coli (in vivo) 3’ His 18 13 18 1
DM2 Cells (cell culture) 3’ His 27 5 18 -
CHO Cells (cell culture) 3’ TAP 23 2 14 11
Protein expression test using 26 “soluble human proteins” and 24 “membrane human proteins”
Table prepared using data from Supplementary Figure 3: “Comparison of activities of various
protein expression systems”: N. Goshima et al., Nature Methods, 5, 1011-1017 (2008)
How does it compare?
Human Protein Factory compared expression of 50 proteins in different systems:
8. 7/18/2013 Matthias Harbers 8
Products and Services for Protein Production
Providing complete solutions to serve customer needs
cDNA Template
mRNA
Expression
Protein
Expression
Protein
Purification
Cloning services SP6 RNA polymerase Standard GST-tag
Gene synthesis RNase inhibitor High-Performance His-tag
PCR services NTP mix GST-tag Tag removal
Sequencing services His-tag
Vectors Protein labeling
Primers - Automated Protein Synthesizers -
9. 7/18/2013 Matthias Harbers 9
pEU-E01 Vector Sets
SP6 promoter for in vitro transcription
E01 enhancer optimized for wheat germ system
Available for 5’ and 3’ tag fusion
Available for PreScisson and/or TEV protease
Do not require 5’ Cap structure or polyadenylation
(GST and His vectors are not offered in the USA and Canada until 2014) (DHFR = dihydrofolate reductase)
Standard Cloning His-TEVGST-TEV/GST-Pre Positive Control
10. 7/18/2013 Matthias Harbers 10
High-Throughput Expression: Split-PCR
ORF5’ 3’
Reverse Primer
Gene Primer
deSP6E01
SPU
ORFE01SP6
Allows for direct use of PCR products in protein synthesis!
ORF5’ 3’
Reverse Primer
5’ 3’
1st PCR
2nd PCR
Split-Primer Pair
~ 1,500 bp
11. 7/18/2013 Matthias Harbers 11
Transcription
SP6 RNA polymerase
Translation
Linked Protein Expression Reactions
The wheat germ system uses separate transcription and translation reactions:
Optimal yields for each reaction means better overall protein yields!
Protein yields depend on RNA quality and amount.
12. 7/18/2013 Matthias Harbers 12
“Bilayer” Translation Reactions
Standard reaction format to allow for extended reaction times and higher yields:
Translation reaction can also be done by dialysis, or automated “Filter-Feed”.
0
1000000
2000000
3000000
4000000
5000000
6000000
7000000
8000000
0 4 8 12 16 20 24
16˚C Plasmid Bilayer 10 DO 16˚C Plasmid Batch 10 OD
26˚C Plasmid Bilayer 10 DO 26˚C Plasmid Batch 10 OD
Optimal reaction temperature at 16℃ for 24h reaction time and high yields.
RFU
Time
13. 7/18/2013 Matthias Harbers 13
Dedicated Wheat Germ Extracts
Crude Protein Yields for Standard Expression Reactions
Application General Use H-Series G-Series Labeling
Product name: WEPRO1240 WEPRO1240H WEPRO1240G WEPRO2240(H)
Protein
analysis
“Standard” His-tag
Fusion Protein
For GST-tag
Fusion Protein
No amino acids
Product name: WEPRO7240 WEPRO7240H WEPRO7240G WEPRO8240(H)
Protein
production
“High
Performance”
His-tag
Fusion Protein
GST-tag
Fusion Protein
No amino acids
Reaction Size WEPRO1240 WEPRO7240 (WEPRO9240)
226 l 15 g 30 g 30 g
1,200 l 100 g 200 g 200 g
6,000 l 500 g 1,000 g 1,000 g
(Data for expression of GFP)
14. 7/18/2013 Matthias Harbers 14
1: WEPRO® 1240 (Purified protein)
2: WEPRO® 7240 (Purified protein)
Up to two-fold higher yield for WEPRO7240!
Comparison WEPRO1240/WEPRO7240
15. 7/18/2013 Matthias Harbers 15
Versions for His- and GST-tagged Proteins
Purified His-tagged protein Purified GST-tagged protein
S: WEPRO® 1240 H: WEPRO® 1240H G: WEPRO® 1240G
(kDa)
97
66
45
30
20
Purity 67% 82%
S G
(kDa)
97
66
45
30
20
Purity 30% 70%
S H
Optimal for the expression and purification of fusion proteins!
(Standard extract) (Pre-cleared extract for His) (Pre-cleared extract for GST)
17. 7/18/2013 Matthias Harbers 17
Premium PLUS Entry Kits (WEPRO9240)
New WEPRO9240 Reagent Kits released in 2013!
CFS developed WEPRO9240 series to address common needs for protein research:
• Optimal for frequent protein synthesis (226 l reactions, up to 30 g protein)
• Cloning vector and Split PCR primers included
• Transcription reagents for 8 reactions included
• Translation reagents for 8 reactions included
• Control template pEU-E01-DHFR included
• Easy to use for setting up cell-free protein synthesis!
18. 7/18/2013 Matthias Harbers 18
Premium PLUS Kit for MS
• Up to 99% labeling efficiency for
incorporating [13C, 15N]-L-Lys and
[13C, 15N]-L-Arg
• Wheat germ cell-free protein
expression (WEPRO9240), proven
for the synthesis of a wide range of
proteins.
• Premixed reagents for rapid
reaction setup of two-step process
• Kit includes cloning vector, primers,
and positive control
Preparation of stable-isotope-labeled protein standards for
quantitative mass spectrometry (SRM/MRM)
Transcription reaction
37℃, 1-4 hours
Translation reaction by Bilayer Method
16℃, 20-24 hours
DNA template
preparation in
pEU-E01 vector
DNA template
preparation by
split-primer
PCR method
or
Cambridge Isotope Laboratories, Inc.
3 Highwood Drive, Tewksbury, MA, 01876, USA.
PH: 800.322.1174
Website: http://www.isotope.com
19. 7/18/2013 Matthias Harbers 19
Scaling Cell-Free Protein Synthesis
WEPRO9240 Reaction Kits 226µl bilayer reactions: <30 µg
WEPRO7240 on Protemist® XE 10-100ml filter-feed reactions: <1g
WEPRO7240 on Protemist® DTII/DTII-S 6ml bilayer reactions: <1000 µg
New dialysis based Products
And Application Protocols
Customer needs/applications
New dialysis based Products
And Application Protocols
Customer needs/applications
20. 7/18/2013 Matthias Harbers 20
Automated Protein Synthesizers
Bilayer
GenDecoder®
HTP Screening
384 samples
Repeat Batch
Protemist®
Mass Production
8 samples
Bilayer
Protemist® DT II/DT II-S
All-in-one Solution
24 samples: Screening
6 samples: Production
Filter-and-Feed
(VivaFlow)
Protemist ® XE
Large-Scale Production
1 sample
(sold out)
Operated for in-house protein synthesis services at CFS!
21. 7/18/2013 Matthias Harbers 21
Protemist® DT II/DT II-S
Tabletop instrument for automated protein synthesis
Performs transcription and translation reactions
Can prepare 24 proteins in parallel on 1.2 ml scale
(Protemist DTII only)
Can prepare 6 proteins in parallel on 6 ml scale
Purification of GST- and His-tagged proteins from 6 ml
reactions
Used for protein screening and large-scale production
Running time: 35 h including protein purification
Used in CFS protein production services
1.2 ml Reactions:
Up to 200 g crude protein
6 ml Reactions:
Up to 1 mg crude protein
Up to 500 g purified protein
(GST or His tag)
22. 7/18/2013 Matthias Harbers 22
Protein Yield Purity
GFP 559 μg 85%
GUS 535 μg 82%
DHFR 549 μg 89%
P38 604 μg 87%
JNK2 490 μg 85%
M: molecular weight
marker
N: negative control
C: crude (3 μl)
E: eluate (0.5 μl)
(kDa)
20 -
25 -
30 -
40 -
50 -
60 -
80 -
100 -
150 -
250 -
C E
GFP BSA (ng)
50 100 250 500M N C E
GUS
C E
DHFR
C E
p38
C E
JNK2
(kDa)
20 -
25 -
30 -
40 -
50 -
60 -
80 -
100 -
150 -
250 -
C E
GFP BSA (ng)
50 100 250 500M N C E
GUS
C E
DHFR
C E
p38
C E
JNK2
Protein Yield Purity
GFP 510 μg 91%
GUS 377 μg 83%
DHFR 520 μg 91%
P38 551 μg 86%
JNK2 383 μg 82%
His-tag protein expression with WEPRO7240H
GST-tag protein expression with WEPRO7240G
M: molecular weight
marker
N: negative control
C: crude (3 μl)
E: eluate (0.5 μl)
Protemist® DT II/DT II-S: Example Data
Protemist DTII is used at CFS to produce >1,500 different proteins every year!
23. 7/18/2013 Matthias Harbers 23
Protemist® XE
Large-scale automated protein synthesis
Using filter-feed method for reagent supply
Up to 10 mg crude protein per ml possible
Can perform 10 to 100 ml reactions
Run time from 24 to 48 h depending on scale
No automated protein purification
Used in CFS protein production services
10 ml Reaction:
Up to 100 mg crude protein
100 ml Reaction:
Up to 1 g crude protein
24. 7/18/2013 Matthias Harbers 24
GenDecoder®
cDNA
Clone Collection
Split-PCR
cDNA
Templates
Transcription Translation
Protein
Samples
384 gene products/over night
Steps performed by GenDecoder®
25. 7/18/2013 Matthias Harbers 25
High-Throughput Screening Assays
ScreeningSplit-PCR
GenDecoder®
(other robotic systems)
384 genes / day
cDNA Libraries
Clone Collections
Protein
Engineering
Protein Expression
384 proteins / day
(GenDecoder®)
384 reactions / few hours
(ALPHA screen™)
384 reactions / day
(FCS)
ALPHA screen™ and FCS analysis equipment are commercially available from PerkinElmer and Olympus respectively.
Feedback for evolutionary design
modification of desired proteins
Examples:
Protein kinase
・ALPHA screen™
Nuclear receptor
・FCS
Vaccine candidates
・ALPHA screen™
・FCS
26. 7/18/2013 Matthias Harbers 26
Protein Production Services
Customer Question/Objective
Cloning resources for template generation
Cell free protein synthesis
QC and production report
Covering all steps from the DNA template to the expressed protein.
Cell-Free expression and automated protein synthesizers allow for fast turnaround times!
27. 7/18/2013 Matthias Harbers 27
Customer Needs Template Preparation Protein Expression
Sequence data? Gene synthesis Solubility testing
cDNA clone? Sub-cloning cDNAs/ORFs Yield testing
cDNA fragments? Cloning of ORF regions QC report
Mutations? Introduction of mutations Optimization
Gene fusions? Cloning of fusion genes Up scaling production
Others… Split-PCR Purification
Inquire about Contract Research at CFS:
Functional Protein Analysis and Screening Assay Development!
Protein Production Services
Template
Preparation
Test
Expression
Production
on different scales
28. • 19,713 coding clones
• Each Clone unique
RefSeq ID
• 14,384 known genes
• 5,329 unknown genes
(as of Nov. 2012)
7/18/2013 Matthias Harbers 28
Annotated Clone Set: Human Gene and Protein Database (HGPD): http://hgpd.lifesciencedb.jp
Human ORF Clones
CFS licensed the right to use the AIST/JBIC Human ORF Clones for commercial purpose.
Input ID or keyword (e.g. “p53”)
→→ output: sequence, protein expression, solubility data, and so on.
“Nearly all proteins synthesized in the wheat germ cell-free expression system
yield soluble fractions”
N. Goshima et al., Human protein factory for converting the transcriptome into an in vitro
expressed proteome, Nature Methods, 5, 1011-1017 (2008)
29. 7/18/2013 Matthias Harbers 29
Human Protein Active Array
• Specific interactions with ~14,000 human proteins can be screened by PAA
platform
• All human proteins are expressed at CFS our wheat germ system
• Use of GST-tag to immobilize proteins on magnetic beads
• Proteins stay in in solution at all times
• No protein denaturation during array production
• Detailed annotation data on human proteins available
PAA Screening Services are available for Contract Research at CFS.
Analyzing the human proteome using comprehensive AIST ORF cDNA collection:
30. 7/18/2013 Matthias Harbers 30
Protein Active Array (PAA)
Assembled PAA Plate
Reaction plate
Magnetic plate
Protein Active Array (PAA) allows screening of entire Human ORF Clone set.
1,536 well format
31. 7/18/2013 Matthias Harbers 31
PAA Standard Protocol
1. Incubate for 1 h with your antibody (concentration 1μg/ml) *
2. Wash the plate with washing buffer
3. Incubate for 1 h with 2nd antibody conjugated with horseradish peroxidase
4. Wash the plate with washing buffer
5. Incubate with chemiluminescence reagent
6. Detect positive proteins by a high sensitive chemiluminescence analyzer
Reagent injection/incubation Detect positive wells by
Image analyzer
Image
Analyzer
Result (image)
* In total 400μg antibody required for entire profiling process
32. 7/18/2013 Matthias Harbers 32
Chemiluminescence image data
14 positive signals on array
(All human proteins are arrayed in duplicate)
Specific Profiling of anti-HA-tag mAB
HA-tag: Synthetic peptide from influenza hemagglutinin epitope: YPYDVPDYA
Screening human protein set for reactivity to commercial anti-HA-tag monoclonal antibody
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
220
160
120
100
90
80
70
60
50
40
30
25
20
15
Lane Entry_Clone_ID kDa
1 FLJ40958AAAF 52.06
2 FLJ10511AAAF 31.28
3 FLJ40876AAAF 25.49
4 FLJ40075AAAF 42.89
5 FLJ92933AAAF 29.41
6 FLJ20516AAAF 34.52
7 FLJ82655AAAF 89.09
8 FLJ10734AAAF 74.67
9 FLJ10882AAAF 68.62
10 FLJ81155AAAF 11.40
11 FLJ31145AAAF 41.99
12 FLJ54287AAAF 25.65
13 FLJ82936AAAF 133.56
14 FLJ83815AAAF 57.51
15 Negative Control
13 antigens out of 14 positive proteins were also detected by Western Blotting.
33. 7/18/2013 Matthias Harbers 33
Detection of Autoantibodies in Cancer
Bladder
cancers
Lung
cancers
Detected only in lung cancers
Detected only in bladder cancersNormal
Detected in both cancers and normal
This research was supported by METI grant No.21R3007C.
34. 7/18/2013 Matthias Harbers 34
Contract Research
CFS is an active partner working closely together with academic research
centers, as well as Biotech and Pharmaceutical Companies on protein related
joint research and development projects:
• Functional protein analysis
• High-throughput compound screening
• Assay development for individual proteins and applications
• New human Protein Active Arrays (PAA) for
Biomarker discovery
Profiling autoimmune antibodies
Antibody specificity screens
Contact us for more information on how we can support your R&D projects.
35. 7/18/2013 Matthias Harbers 35
Basic to Technology (Description of the technology)
Takai, T., Sawasaki, S., and Endo, Y. (2010) Practical cell-free protein synthesis system using purified wheat embryos,
Nat. Protoc., 5, 227-238.
X-ray Crystallography (Using CellFree Sciences Products)
Beebe, E.T., Makino, S., Nozawa, A., Matsubara, Y., Frederick, R.O., Primm, J.G., Goren, M.A., and Fox, B.G. (2010)
Robotic large-scale application of wheat cell-free translation to structural studies including membrane proteins. New
Biotechnol., 28, 239-249
Guild, K., Zhang, Y., Stacy, R., Mundt, E., Benbow, S., Green, A., and Myer, P.J. (2011) Wheat germ cell-free expression
system as a pathway to improve protein yield and solubility for the SSGCID pipeline. Acta Crystallogr. F., 67, 1027–
1031
Mass Spectrometry (Using CellFree Sciences Products)
Singh, S., Springer, M., Steen, J., Kirschner, M.W., and Steen, H. (2009) FLEXIQuant: A novel tool for the absolute
quantification of proteins, and the simultaneous identification and quantification of potentially modified peptides. J
Proteome Res., 8, 2201 - 2210
Biotinylation (Using CellFree Sciences Products)
Matsuoka, K., Komori, H., Nose, M., Endo, Y., and Sawasaki, T. (2010) Simple screening method for autoantigen
proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. J Proteime Res., 9,
4264-4273
Selected References
36. 7/18/2013 Matthias Harbers 36
iPS Cell Reseach (Using CellFree Sciences Products)
Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, Ichisaka T, Kawamura Y, Mochizuki H, Goshima N,
Yamanaka S. Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature. 2011
Jun 8;474(7350):225-9. doi: 10.1038/nature10106.
Expression of Membrane Proteins (Using CellFree Sciences Products)
Nozawa, A., Ogasawara, T., Matsunaga, S., Iwasaki, T., Sawasaki, T., and Endo, Y. (2011) Production and partial
purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. BMC
Biotechnol., 11:35
Jarecki BW, Makino S, Beebe ET, Fox BG, Chanda B. Function of Shaker potassium channels produced by cell-free
translation upon injection into Xenopus oocytes.
Sci Rep. 2013;3:1040. doi: 10.1038/srep01040. Epub 2013 Jan 8.
Recent Reviews on Cell-Free Protein Synthesis
Carlson ED, Gan R, Hodgman CE, Jewett MC. Cell-free protein synthesis: applications come of age.
Biotechnol Adv. 2012 Sep-Oct;30(5):1185-94.
Casteleijn MG, Urtti A, Sarkhel S. Expression without boundaries: cell-free protein synthesis in pharmaceutical
research. Int J Pharm. 2013 Jan 2;440(1):39-47. doi: 10.1016/j.ijpharm.2012.04.005. Epub 2012 Apr 11.
Selected References
37. 7/18/2013 Matthias Harbers 37
Learn more about CellFree Sciences
Follow us on LinkedIn: http://www.linkedin.com/company/3139350?trk=tyah
Visit our homepage at: http://www.cfsciences.com/eg/index.html
Reagents for cell-free protein expression
Protein synthesizers
Protein expression services
Protein Active Array
Contract research for protein studies
38. 7/18/2013 Matthias Harbers
Thank you for working with CFS!
38
Dr. Matthias Harbers
E-mail: m-harbers@cfsciences.com
Tel.:+81-45-500-2119 FAX:+81-45-500-2117