Sex, guts and healthy bones
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This document discusses a study examining the links between intestinal inflammation, sex, and bone health in mice. The study found that male and female mice responded differently to intestinal inflammation caused by the bacteria Helicobacter hepaticus, with males experiencing greater bone loss. The study also found that the probiotic bacterium Lactobacillus reuteri reduced intestinal inflammation and bone loss in male mice, indicating sex determines the intestinal and bone responses to both harmful and beneficial bacteria.
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Sex, guts and healthy bones
- 1. Sex, guts and healthy bones Laura McCabe, Ph.D. Departments of Physiology and Radiology February 26, 2016
- 2. Sex, guts and healthy bones Laura McCabe, Ph.D. Departments of Physiology and Radiology, MSU Inflammation GEORGE JEN Intestinal TNF
- 3. IL-10 -/- ♂ ♀ Adult mice *** *** 0 0.7 1.4 Male BoneVolume/ BodyMass Control H. hep Control H. hep *** ** 2-way ANOVA Gender p ≤ 0.001 H. hep p ≤ 0.001 Gender X H.hep p ≤ 0.001 MALEFEMALEControl H.hep Femalele A ** Control H.hep FemaleMale A B 2-wayANOVA Gender p≤0.05 H.hep p≤0.001 Sex determines intestinal and bone responses to “bad” bacteria H. Hepaticus Bonedensity
- 4. BoneDensity Intestinal Inflammation is Linked to Bone Loss Colon TNFa levels r = -0.79 p < 0.0001 0 30 60 0 5 10 Intestinal Pathology Score r = -0.76 p = 0.002 0 30 60 0 12 24
- 5. • In milk, meat, human gut • Used to treat colic • Produces anti-microbials • Anti-inflammatory activity 0 8 16 24 32 MALEBonedensity * Control +Lr * 47% MALEIntestinal TNFα/HPRT 0 0.2 0.4 0.6 0.8 1 control lacto Control +Lr Control ↓E ↓E+Lr Vertebrae 0 10 20 FEMALE Bonedensity Lactobacillus reuteri (LR): Sex determines intestinal and bone responses to “good” bacteria
- 6. Inflammation Immune cells Bacteria + products Blood Intestinal EpithelialBarrier Tight junctions Gut environment modulators: Probiotics, Prebiotics, Antibiotics, New Compounds Other signals: Incretins, Serotonin, Neural, Distal Inflammation Working Model Bone marrow
Editor's Notes
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- Mice 14-18 week, 109 cfus Inhibits gram- bacteria Humans sometimes need supplementation to maintain high levels in gut http://www.siliconvalleyfit.com/blog/bid/347252/Can-L-Reuteri-Lower-Your-LDL-Cholesterol Gram postive Biogsis owns several strains of this Lactic acid producing bacteria Find in food, meat and milk products L. Reuteri found in human stool in 1960s but also in sheep, chickens, pigs and rodents Host-Specific strains for each aniaml Produces reuterin, reutericin 6 and reutericyclin – antimicrobials Can inhibit gram neg bacteria Humans don’t always have l reuteri and need supplementation to maintain high levels in gut Tested in children, healthy adutls and immunosuppressed HIV patients without any adverse effects with 10 to 10 dose units per day. Found to reudce motor complex and motility Very potent at reducing gut infections Very effective against colic – not clear why Commerical turkeys have increased weight when give LR…However, other studies have revealed it can also help when the growth depression is caused entirely by a lack of dietary protein, and not by contagious disease.[51] This raises the possibility that L. reuteri somehow improves the intestines' ability to absorb and process nutrients.[12] Low GC content Journal of Pediatr Gastroenterol Nutr. 2009 Sep;49(3):349-54 Lactobacilli in breast milk and detected in infants The probiotic that I’ll be focusing on today is…Currently, we have established genetic tools in the lab that will aid in investigating the beneficial functions of L. reuteri. And lastly, there have been demonstrated immunomodulatory activity for this strain.