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The Effect of CHO and PRO
Supplementation and the Timing of
Ingestion on Muscle Recovery and
Performance in Endurance Athletes
Written By: Lauren Luketic

EXPH 496
Outline
•

Introduction
– Background
– Thesis Question

Significance of the Problem
• Review of the Literature
•

– Pre-Competition
– During Competition
– Post-Competition
Outline: Cont.’d
Critical

Analysis of the Literature
Conclusion
Future Research
Questions
Introduction
Background

– Nutrition + Training = Performance
– Energy balance
– Nutritional supplementation
• Carbohydrate (CHO)
• Protein (PRO)

– Maintaining blood glucose
• CHO loading

– Exercise-induced muscle damage (EIMD)
Introduction: cont.’d
Thesis

Question:

– How does the timing of
carbohydrate and/or protein
supplementation affect
performance and muscle recovery
in endurance athletes?
Significance of the Problem
Benefits

athletes on all levels of
competition

Ultimate

goal:

◦ Optimize performance
◦ Increase rate of muscle recovery
Prevention

of serious consequences
Significance of the Problem: cont.’d
Consequences

◦ Negative energy balance
 Metabolic dysfunctions
 Loss of lean muscle mass

 ↓ Strength and endurance
◦ Female athletes
 Amenorrhea
 Osteoporosis

◦ Potential for success & overall health
would be negatively affected
Review of the Literature
Supplementation

type ↔ timing of

ingestion
Hypothesis:

◦ If ingestion of supplements before and
during exercise increases performance
and delays fatigue, then EIMD would be
less making supplementation after
exercise more efficient.
◦ ↓ EIMD = ↑ recovery rate
Review of the Literature: cont.’d
Pre-competition

◦ CHO loading = ↑ performance
During

competition

◦ Sports drinks (CHO + electrolytes)
◦ Delay on-set of fatigue
◦ Keep optimal pace
Post-competition

◦ PRO = ↑ rate of muscle recovery
Critical Analysis of the Literature
Conflicting

results between studies

◦ CHO+PRO (before, during, & after)
◦ High vs. Low GI meals
Lack

of female subjects
Conclusion
Best

supplement

◦ Pre-competition
◦ During competition
◦ Post-competition
Overall

best supplement

◦ Inconclusive!!!
Future Research
Include
Other

more women

ergogenic aids

◦ Caffeine
◦ Creatine
◦ Chocolate milk
Dose

and frequency ratio
Questions?
References
1.

2.

3.

4.

5.

6.

American Dietetic Association, Dietitians of Canada, American College of
Sports Medicine. Joint Position Statement: Nutrition and Athletic
Performance. Med Sci Sports Exerc. 41(3):709-731, 2009.
Berardi JM, et al. Postexercise muscle glycogen recovery enhanced with a
carbohydrate-protein supplement. Med Sci Sports Exerc. 38(6):1106-1113,
2006.
Berardi JM, Noreen EE, and Lemon PWR. Recovery from a cycling time trial
is enhanced with carbohydrate-protein supplementation vs. isoenergetic
carbohydrate supplementation. J Int Soc Sports Nutr. 5:24, 2008.

Betts JA, et al. Systemic indices of skeletal muscle damage and
recovery of muscle function after exercise: effect of combined
carbohydrate-protein ingestion. Appl Physiol Nutr Metab. 34(4):773784, 2009.
Blacker, et al. Carbohydrate vs protein supplementation for recovery
of neuromuscular function following prolonged load carriage. J Int
Soc Sports Nutr. 7:2, 2010.
Campbell C, et al. Carbohydrate-supplement form and exercise
performance. Int J Sport Nutr Exerc Metab. 18(2):179-190, 2008.
References: Cont.’d
7.

8.

9.

10.

11.

12.

Carrithers JA, et al. Effects of postexercise carbohydrate-protein
feedings on muscle glycogen restoration. J Appl Physiol. 88:19761982, 2000.
Chen YJ, et al. Effect of preexercise meals with different glycemic
indices and loads on metabolic responses and endurance running.
Int J Sport Nutr Exerc Metab. 18(3):281-300, 2008.
Chwalbiñska-Moneta J. Effect of creatine supplementation on
aerobic performance and anaerobic capacity in elite rowers in the
course of endurance training. Int J Sport Nutr Exerc Metab.
13(2):173-183, 2003.
Dadgostar H, et al. The relation between athletic sports and
prevalence of amenorrhea and oligomenorrhea in Iranian female
athletes. Sports Medicine, Arthroscopy, Rehabilitation, Therapy &
Technology. 1:16, 2009.
De Bock K, et al. Fiber type-specific muscle glycogen sparing due
to carbohydrate intake before and during exercise. J Appl Physiol.
102:183-188, 2007.
Desbrow B, et al. Carbohydrate-electrolyte feedings and 1 h time
cycling performance. Int J Sport Nutr Exerc Metab. 14(5):541-549,
2004.
References: Cont.’d
13.

14.

15.

16.

17.

18.
19.

Etheridge T, Philp A, Watt PW. A single protein meal increases
recovery of muscle function following an acute eccentric exercise
bout. Appl Physiol Nutr Metab. 33(3):483-488, 2008.
Green MS, et al. Carbohydrate-protein drinks do not enhance
recovery from exercise-induced muscle injury. Int J Sport Nutr
Exerc Metab. 18(1):1-18, 2008.
Howarth KR, et al. Coingestion of protein with carbohydrate during
recovery from endurance exercise stimulates skeletal muscle
protein synthesis in humans. J Appl Physiol. 106:1394-1402,
2009.
Howatson G, van Someren KA. The prevention and treatment of
exercise-induced muscle damage. Sports Med. 38(6):483-503,
2008.
Ivy JL, et al. Early postexercise muscle glycogen recovery is
enhanced with a carbohydrate-protein supplement. J Appl Physiol.
93:1137-1344, 2002.
Kerksick C, et al. International Society of Sports Nutrition position
stand: Nutrient timing. J Int Soc Sports Nutr. 5:17, 2008.
Kern M, Heslin CJ, Rezende RS. Metabolic and performance
effects of raisins versus sports gel as pre-exercise feedings in
cyclists. J Strength Cond Res. 21(4):1204-1207, 2007.
References: Cont.’d
20.

21.

22.

23.

24.

25.

26.

Khanna GL, Manna I. Supplementary effect of carbohydrateelectrolyte drink on sports performance, lactate removal &
cardiovascular response of athletes. Indian J Med Res. 121:665669, 2005.
Martínez-Lagunas V, et al. Added protein maintains efficacy of a
low-carbohydrate sports drink. J Strength Cond Res. 24(1):48-59,
2010.
Moore LJ, et al. Effect of the glycaemic index of a pre-exercise
meal on metabolism and cycling time trial performance. J Sci Med
Sport. 13(1):182-188, 2010.
Pedersen DJ, et al. High rates of muscle glycogen resynthesis
after exhaustive exercise when carbohydrate is coingested with
caffeine. J Appl Physiol. 105:7-13, 2008.
Rauch HGL, et al. A signalling role for muscle glycogen in the
regulation of pace during prolonged exercise. Br J Sports Med.
39:34–38, 2005.
Rollo I, Williams C. Influence of ingesting a carbohydrateelectrolyte solution before and during a 1-hr running performance
test. Int J Sport Nutr Exerc Metab. 19(6):645-658, 2009.
Sewell DA, Robinson TM, Greenhaff PL. Creatine supplementation
does not affect human skeletal muscle glycogen content in the
absence of prior exercise. J Appl Physiol. 104:508-512, 2008.
References: Cont.’d
27.

28.

29.

30.

Skillen RA, et al. Effects of an amino acid carbohydrate
drink on exercise performance after consecutive-day
exercise bouts. Int J Sport Nutr Exerc Metab. 18(5):473492, 2008.
Slivka D, et al. Caffeine and carbohydrate
supplementation during exercise when in negative energy
balance: effects on performance, metabolism, and salivary
cortisol. Appl Physiol Nutr Metab. 33(6):1079-1085, 2008.
Stellingwerff T, et al. Carbohydrate supplementation
during prolonged cycling exercise spares muscle glycogen
but does not affect intramyocellular lipid use. Eur J
Physiol. 454:635-647, 2007.
Stevenson EJ, et al. Influence of high-carbohydrate mixed
meals with different glycemic indexes on substrate
utilization during subsequent exercise in women. Am J
Clin Nutr. 84:354–60, 2006.
References: Cont.’d
31.

32.

33.

34.

35.

Valentine RJ, et al. Influence of carbohydrate-protein
beverage on cycling endurance and indices of muscle
disruption. Int J Sport Nutr Exerc Metab. 18(4):363-378,
2008.
White JP, et al. Effect of carbohydrate-protein supplement
timing on acute exercise-induced muscle damage. J Int
Soc Sports Nutr. 5:5, 2008.
Wong SH, et al. Effect of glycemic index meals on
recovery and subsequent endurance capacity. Int J
Sports Med. 30(12):898-905, 2009.
Wong SH, et al. Effect of preexercise glycemic-index meal
on running when CHO-electrolyte solution is consumed
during exercise. Int J Sport Nutr Exerc Metab. 19(3):222242, 2009.
Yeo SE, et al. Caffeine increases exogenous
carbohydrate oxidation during exercise. J Appl Physiol.
99:844-850, 2005.

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"The Effect of Carbohydrate and Protein Supplementation and the Timing of Ingestion on Muscle Recovery and Performance in Endurance Athletes"

  • 1. The Effect of CHO and PRO Supplementation and the Timing of Ingestion on Muscle Recovery and Performance in Endurance Athletes Written By: Lauren Luketic EXPH 496
  • 2. Outline • Introduction – Background – Thesis Question Significance of the Problem • Review of the Literature • – Pre-Competition – During Competition – Post-Competition
  • 3. Outline: Cont.’d Critical Analysis of the Literature Conclusion Future Research Questions
  • 4. Introduction Background – Nutrition + Training = Performance – Energy balance – Nutritional supplementation • Carbohydrate (CHO) • Protein (PRO) – Maintaining blood glucose • CHO loading – Exercise-induced muscle damage (EIMD)
  • 5. Introduction: cont.’d Thesis Question: – How does the timing of carbohydrate and/or protein supplementation affect performance and muscle recovery in endurance athletes?
  • 6. Significance of the Problem Benefits athletes on all levels of competition Ultimate goal: ◦ Optimize performance ◦ Increase rate of muscle recovery Prevention of serious consequences
  • 7. Significance of the Problem: cont.’d Consequences ◦ Negative energy balance  Metabolic dysfunctions  Loss of lean muscle mass  ↓ Strength and endurance ◦ Female athletes  Amenorrhea  Osteoporosis ◦ Potential for success & overall health would be negatively affected
  • 8. Review of the Literature Supplementation type ↔ timing of ingestion Hypothesis: ◦ If ingestion of supplements before and during exercise increases performance and delays fatigue, then EIMD would be less making supplementation after exercise more efficient. ◦ ↓ EIMD = ↑ recovery rate
  • 9. Review of the Literature: cont.’d Pre-competition ◦ CHO loading = ↑ performance During competition ◦ Sports drinks (CHO + electrolytes) ◦ Delay on-set of fatigue ◦ Keep optimal pace Post-competition ◦ PRO = ↑ rate of muscle recovery
  • 10. Critical Analysis of the Literature Conflicting results between studies ◦ CHO+PRO (before, during, & after) ◦ High vs. Low GI meals Lack of female subjects
  • 11. Conclusion Best supplement ◦ Pre-competition ◦ During competition ◦ Post-competition Overall best supplement ◦ Inconclusive!!!
  • 12. Future Research Include Other more women ergogenic aids ◦ Caffeine ◦ Creatine ◦ Chocolate milk Dose and frequency ratio
  • 14. References 1. 2. 3. 4. 5. 6. American Dietetic Association, Dietitians of Canada, American College of Sports Medicine. Joint Position Statement: Nutrition and Athletic Performance. Med Sci Sports Exerc. 41(3):709-731, 2009. Berardi JM, et al. Postexercise muscle glycogen recovery enhanced with a carbohydrate-protein supplement. Med Sci Sports Exerc. 38(6):1106-1113, 2006. Berardi JM, Noreen EE, and Lemon PWR. Recovery from a cycling time trial is enhanced with carbohydrate-protein supplementation vs. isoenergetic carbohydrate supplementation. J Int Soc Sports Nutr. 5:24, 2008. Betts JA, et al. Systemic indices of skeletal muscle damage and recovery of muscle function after exercise: effect of combined carbohydrate-protein ingestion. Appl Physiol Nutr Metab. 34(4):773784, 2009. Blacker, et al. Carbohydrate vs protein supplementation for recovery of neuromuscular function following prolonged load carriage. J Int Soc Sports Nutr. 7:2, 2010. Campbell C, et al. Carbohydrate-supplement form and exercise performance. Int J Sport Nutr Exerc Metab. 18(2):179-190, 2008.
  • 15. References: Cont.’d 7. 8. 9. 10. 11. 12. Carrithers JA, et al. Effects of postexercise carbohydrate-protein feedings on muscle glycogen restoration. J Appl Physiol. 88:19761982, 2000. Chen YJ, et al. Effect of preexercise meals with different glycemic indices and loads on metabolic responses and endurance running. Int J Sport Nutr Exerc Metab. 18(3):281-300, 2008. Chwalbiñska-Moneta J. Effect of creatine supplementation on aerobic performance and anaerobic capacity in elite rowers in the course of endurance training. Int J Sport Nutr Exerc Metab. 13(2):173-183, 2003. Dadgostar H, et al. The relation between athletic sports and prevalence of amenorrhea and oligomenorrhea in Iranian female athletes. Sports Medicine, Arthroscopy, Rehabilitation, Therapy & Technology. 1:16, 2009. De Bock K, et al. Fiber type-specific muscle glycogen sparing due to carbohydrate intake before and during exercise. J Appl Physiol. 102:183-188, 2007. Desbrow B, et al. Carbohydrate-electrolyte feedings and 1 h time cycling performance. Int J Sport Nutr Exerc Metab. 14(5):541-549, 2004.
  • 16. References: Cont.’d 13. 14. 15. 16. 17. 18. 19. Etheridge T, Philp A, Watt PW. A single protein meal increases recovery of muscle function following an acute eccentric exercise bout. Appl Physiol Nutr Metab. 33(3):483-488, 2008. Green MS, et al. Carbohydrate-protein drinks do not enhance recovery from exercise-induced muscle injury. Int J Sport Nutr Exerc Metab. 18(1):1-18, 2008. Howarth KR, et al. Coingestion of protein with carbohydrate during recovery from endurance exercise stimulates skeletal muscle protein synthesis in humans. J Appl Physiol. 106:1394-1402, 2009. Howatson G, van Someren KA. The prevention and treatment of exercise-induced muscle damage. Sports Med. 38(6):483-503, 2008. Ivy JL, et al. Early postexercise muscle glycogen recovery is enhanced with a carbohydrate-protein supplement. J Appl Physiol. 93:1137-1344, 2002. Kerksick C, et al. International Society of Sports Nutrition position stand: Nutrient timing. J Int Soc Sports Nutr. 5:17, 2008. Kern M, Heslin CJ, Rezende RS. Metabolic and performance effects of raisins versus sports gel as pre-exercise feedings in cyclists. J Strength Cond Res. 21(4):1204-1207, 2007.
  • 17. References: Cont.’d 20. 21. 22. 23. 24. 25. 26. Khanna GL, Manna I. Supplementary effect of carbohydrateelectrolyte drink on sports performance, lactate removal & cardiovascular response of athletes. Indian J Med Res. 121:665669, 2005. Martínez-Lagunas V, et al. Added protein maintains efficacy of a low-carbohydrate sports drink. J Strength Cond Res. 24(1):48-59, 2010. Moore LJ, et al. Effect of the glycaemic index of a pre-exercise meal on metabolism and cycling time trial performance. J Sci Med Sport. 13(1):182-188, 2010. Pedersen DJ, et al. High rates of muscle glycogen resynthesis after exhaustive exercise when carbohydrate is coingested with caffeine. J Appl Physiol. 105:7-13, 2008. Rauch HGL, et al. A signalling role for muscle glycogen in the regulation of pace during prolonged exercise. Br J Sports Med. 39:34–38, 2005. Rollo I, Williams C. Influence of ingesting a carbohydrateelectrolyte solution before and during a 1-hr running performance test. Int J Sport Nutr Exerc Metab. 19(6):645-658, 2009. Sewell DA, Robinson TM, Greenhaff PL. Creatine supplementation does not affect human skeletal muscle glycogen content in the absence of prior exercise. J Appl Physiol. 104:508-512, 2008.
  • 18. References: Cont.’d 27. 28. 29. 30. Skillen RA, et al. Effects of an amino acid carbohydrate drink on exercise performance after consecutive-day exercise bouts. Int J Sport Nutr Exerc Metab. 18(5):473492, 2008. Slivka D, et al. Caffeine and carbohydrate supplementation during exercise when in negative energy balance: effects on performance, metabolism, and salivary cortisol. Appl Physiol Nutr Metab. 33(6):1079-1085, 2008. Stellingwerff T, et al. Carbohydrate supplementation during prolonged cycling exercise spares muscle glycogen but does not affect intramyocellular lipid use. Eur J Physiol. 454:635-647, 2007. Stevenson EJ, et al. Influence of high-carbohydrate mixed meals with different glycemic indexes on substrate utilization during subsequent exercise in women. Am J Clin Nutr. 84:354–60, 2006.
  • 19. References: Cont.’d 31. 32. 33. 34. 35. Valentine RJ, et al. Influence of carbohydrate-protein beverage on cycling endurance and indices of muscle disruption. Int J Sport Nutr Exerc Metab. 18(4):363-378, 2008. White JP, et al. Effect of carbohydrate-protein supplement timing on acute exercise-induced muscle damage. J Int Soc Sports Nutr. 5:5, 2008. Wong SH, et al. Effect of glycemic index meals on recovery and subsequent endurance capacity. Int J Sports Med. 30(12):898-905, 2009. Wong SH, et al. Effect of preexercise glycemic-index meal on running when CHO-electrolyte solution is consumed during exercise. Int J Sport Nutr Exerc Metab. 19(3):222242, 2009. Yeo SE, et al. Caffeine increases exogenous carbohydrate oxidation during exercise. J Appl Physiol. 99:844-850, 2005.