This document discusses lung cancer and the role of the protein WDR72. It describes the two main types of lung cancer as non-small cell lung cancer (NSCLC), which accounts for 80-85% of cases, and small cell lung cancer (SCLC), which spreads faster but responds better to chemotherapy. The objective is to investigate how WDR72 influences lung cancer cell stemness and provide new treatment strategies. Methods like luciferase assays, RT-qPCR, western blot, and apoptosis assays are used to quantify protein and gene expression levels. Knocking down WDR72 expression suppresses lung cancer growth and metastasis via the AKT/HIF-1α signaling pathway, suggesting WDR72 could be a new

1. THIRD SEMESTER - MEDICINE
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2. What is a lung cancer
● Its when the cells start growing without any control in the lungs, the American Cancer Association
describes two types of lung cancer no small cell lung cancer(NSCLC) and small cell lung cancer
● NSCLC
○ Its 80% to 85% of lung cancer
○ The main subtypes of NSCLC are adenocarcinoma, squamous cell carcinoma, and large cell
carcinoma.
● SCLC
○ Its the 10% to 15% of all lung cancer
○ Its spread faster but responds better to chemoterapy and radiation terapy

3. SIGNAL RUTE
https://pubmed.ncbi.nlm.nih.gov/30106145/

4. OBJECTIVE
Investigate the influence of WDR72 on the stemness of lung cancer cells and provide a new
treatment strategy for lung cancer

5. methods
○ 2,3 Luciferase Assay. Signal Finder 10-Pathway
Reporter: it’s a method that determinates the presence
of ATP, Mg and O2
○ 2,5 RT-qPCR.: its a real time methods how have the
objective of measurement of WDR72, OCT4, CD44,
CD133, Cyclin A1, Cyclin B1, E-cadherin, Ncadherin,Bax,
and Bcl-2.

6. methods
○ 2,7 Western Blot and Immunohistochemistry (IHC): its a
technique that has the reason of quantify the
expression of a given number of proteins
○ 2,9 Cell Apoptosis Assay its a technique that
detects and quantify apoptosis associated events
https://www.cancer.gov/types/lung/patie
nt/non-small-cell-lung-treatment-pdq

7. RESULTS

8. RESULTS

9. Authors Author words Agree or disagree
M. Karami Fath Aberrant activation of the
PI3K/AKT pathway enhances
characteristics of tumors such as
increased proliferation, cell cycle
progression, metastasis, and
inhibition
of apoptosis
I. G. Kim, S. Y. Kim, S.
I.
PI3K is activated when cells are
stimulated by
external stimuli and
subsequently, PI3K catalyzes
the production of PIP3, which
binds to AKT and
phosphorylates it
A. D. Choudhury In lung cancer, the PI3K/Akt
signaling
pathway is frequently
overactivated

10. Conclusion
○ The WDR72 is a overexpressed protein in lung
cancer by knocking down the WDR72 we can
suppress the growing an metastasis of the lung
cancer via AKT/HIF-1α signaling pathway
○ The molecular biology is very important because
permit us to see and detect any type of unbalance
and show us the way of treatment of diagnosis
https://dean.clas.ufl.edu/interdisciplinary-studies/established-
concentrations/biochemistry-and-molecular-biology/