2. sars-cov-2
It is the virus that causes a respiratory
disease called coronavirus disease,
which first appeared in 2019 and then
represented a global challenge as it
reached the level of a pandemic.
INTRODUCTION
3. IntegrIns
They are heterodimeric
transmembrane proteins
composed of α and β
subunits.
The article investigates
the role of integrins,
particularly integrin αvβ1,
in facilitating the entry of
SARS-CoV-2 mediated by
ACE2.
INTRODUCTION
4. OBJECTIVE
This study aimed to investigate how integrins facilitate the
ACE2-mediated cellular entry of SARS-CoV-2.
5. CEll lines and cell
culture co-transfection
methods
Human cell lines:
293T
SW620
IOSE-80
MDA-MB-231
HepG2
PANC-1
HGC-27
BEAS-2B
Calu-3
Refers to the
simultaneous
transfection of two
separate nucleic acid
molecules into a cell,
allowing each to carry
out its function within
the same cell.
Mouse cells:
NIH3T3
6. pcr
WESTERN BLOTTING
methods
The mRNA expression analysis
was conducted using reverse
transcription quantitative real-
time PCR (RT-qPCR).
Is a laboratory technique used to
detect a specific protein in a
blood or tissue sample.
7. results
Shows the protein
expression level of integrin
ITGAV, ITGB1, and ACE2 in
indicated cell lines analyzed.
The results indicate the
presence and relative levels
of these proteins in the
tested cell lines.
8. results
Showed that SARS-CoV-2 RBD exhibited potent
binding to integrin αvβ1, whereas the association of
RGD (403–405)→AAA variant RBD with integrin αvβ1
was dramatically reduced.
Showed that the full-length S proteins (~180 kDa) were
detected in both the WT RGD and RGD (403–405)→AAA
pseudotyped virions, but the level of the mutant RGD
was markedly reduced compared with the WT one.
9. Authors What did the authors said Agreement
Yan et al., 2020
“The RGD motif in the spike protein of SARS-CoV-2 has been suggested to
be essential element for virus internalization”
Agree
Caccuri et al., 2021; Gao
et al., 2021; Luan et al.,
2020; Sigrist et al., 2020
“A number of studies have demonstrated that integrins on the cellsurface
may contribute to the high affnity of viral binding of the SARS-CoV-2, but
the exact role of integrins in ACE2-mediated SARSCoV-2 entry appears
controversial, varying from the independent cellular receptor competing
with ACE2 to the facilitator synergizing with ACE2”
Agree
Gullberg et al., 2013;
Hernandez ´ et al., 1996
“Interestingly, the RGD (403–405)→AAA mutation not only markedly
affected the viral entry, but also the cleavage of the spike protein of SASR-
CoV-2. Previous studies reported that Foot-and-mouth disease virus
(FMDV) was cleaved in situ by trypsin at the RGD motif”
Agree
DISCUSSION
10. In the context of this article, molecular biology is fundamental, since it
provides a detailed understanding of biological processes at the molecular
level, in this case it allows the identification of biomarkers to diagnose
diseases and the understanding of the body's response to diseases.
Molecular biology is crucial for the diagnosis, treatment and prevention of
diseases, and is also essential for advancing in personalized medicine, gene
therapy, and the development of more effective and specific treatments.
CONCLUSIONS
