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WELCOM
E
Extraction of Pectin from Lemon and Orange Fruits Peels and Its Utilization
in Jam Making.
Prepared by
Gohel Savan
321
Introductio
n
Materials &
method
Conclusion
4
conclusionResult &
discussion
Introduction
Oranges
Oranges are a very good source of vitamins,
especially vitamin C
grew first in South and East Asia but now grows in many parts
of the world.
Orange is most commonly grown fruit in the world.
From peels of Oranges “D-Limonene oil” & “Pectin” is
extracted.
Outer part of peel is source of “D-limonene Oil”.
Inner Part of peel known as “Albedo” is source of Pectin.
Lemon
 Lemon (Citrus × limon ) is one of the important citrus fruits
appreciably not only for its beautiful appearance and pleasing
flavor but also for excellent food qualities.
 the production area of lemon fruits in India is 258 (000'HA)
while the production is 2978 (000'MT) (NHB database-2015-
16).
 Lemon belongs to family Rutaceae. It is cultivated widely in
the tropical and subtropical regions.
 The origin of lemon fruit is Asia..
Pectin
 Pectin, in general, is a group of polysaccharides found in
nature in the primary cell walls of all seed bearing plants and
are invariably located in the middle lamella.
“D-galacturonic acid”
 Pectin Contains “neutral sugars” (xylose, galactose &
arabinose ) which is present in Side Chains..
 It was first isolated in 1825 by Henri Braconot.
One of the richest sources of pectin is lemon or orange
which contains about 30% of this polysaccharide.
Pectin is naturally found in a number of plants : lemon peel,
orange peel, apple pomace, carrots, sunflower-heads, guava,
mangoes and papaya.
Jam
Jam is a product made by boiling fruit pulp with sufficient
sugar to a reasonably thick consistency, firm enough to hold
the fruit tissues in position .
Lemon ,orange , Apple, pear, sapota (chiku),peach, papaya,
carrot, straw- berry, raspberry, mango, tomato, grapes and
muskmelon are used for preparation of jams.
It can be prepared from one kind of fruit or from two or more
kinds .
Jam contains 0.5-0.6 per cent acid and sugar should not be
more than 40 percent and TSS 68%.
Typical levels of pectin in plants are:
Apples - 1-1.5%
Apricots - 1%
Cherries - 0.5-3.5%
Carrots approx. - 1.4%
Citrus peels - 30%
Materials and methods
Extraction of Pectin
Orange and lemon were peeled and dried for four days and powdered.
Orange powder (500 g) and from lemon powder (500 g) were used.
Then 5 liter distilled water and 50 ml HCL were added for each blend and then
mixed and left for 24 hours, then filtered in separation device.
 One liter of filtrate was added to 1 liter ethanol (95%), the mixture was put into
centrifugation apparatus. Ten left one hour and filtered through Buchner funnel.
The filtrate was washed with 250 ml acetone for drying and filtrate was dried at
room temperature for 24 hours. The product was ground into fine powder and sieved
by 40 mesh sieve to separate pectin from fiber. The pectin powder was then
collected, weighed and packed in plastic container pending jam production.
Flow Sheet For Extraction
Orange & lemon
Peel Filter Cake Ethanol
Sample
Mother Liquor Water Bath Precipitation of
+ Filtration
Heating pectin
Citric Acid
DRY PECTIN Drying Jelly Pectin Centrifuge
Jelly Pectin Dry Pectin
Few Indian Major Players are as under pectin
Extraction
 Krishna pectins pvt. Ltd.
Devson Impex Private Limited
Gujarat General Food Chem Pvt. Ltd.
Asha Ram & Sons Private Limited
Sr. no. Observation Quantity
1 Distilled Water 5 lit
2
Amount of Orange & lemon peels
powder 500 gm + 500 gm
3 Distillate obtained(Oil) 20 ml
4 Acetone 250 ml
Experimental Observations
Processing of Jam
Results and Discussion
Table 1. Amounts and percentages of pectin extracted from orange and
lemon
Quantity ,gm Weight of
pectin
Percentage of
pectin
Lemon 500 gm 31.5g 15.25 %
orange 500 gm 41.5g 20.75 %
Table 2. Chemical composition of orange pectin jam; lemon pectin
jam
OPJ: orange pectin jam; LPJ: lemon pectin jam; CPJ: commercial
pectin jam
Parameters Extracted pectin jam Commercial pectin
jam
OPJ LPJ CPJ
pH 4.20 3.50 3.90
TSS 60.0 61.0 57.0
Titrable acidity % 0.49 0.38 0.37
Moisture % 29.0 28.0 30.0
Ash % 0.36 0.35 0.32
Carbohydrate 62.0 66.02 61.83
Vitamin C (mg/100g) 35.0 51 22.0
Table 3. Minerals contents of orange pectin jam , lemon pectin jam
and
commercial pectin jam
OPJ: orange pectin jam; LPJ: lemon pectin jam; CPJ: commercial
pectin jam
Minerals OPJ LPJ CPJ
Sodium mg/100g 188 160 88
Potassium
mg/100g
148 153 86
Calcium mg/100g 35 24 17
Table 4. Sensory evaluation scores* of orange pectin jam , lemon pectin jam
commercial pectin jam
Jam Colour &
appearanc
e
texture flavour Overall
acceptability
OPJ 6.2b 6.8b 7.3b 7.1b
LPJ 7.1b 6.5b 8.2a 6.9b
CPJ 8.5a 8.2a 8.6a 8.4a
Conclusions
The low gel strength of the jam can be improved by the addition of pectin
during processing to attain the commercially acceptable gel strength or a
combination of fruits rich in pectin can be used to make up for the
deficiency.
The combination with other fruits could serve to improve the flavour and
the colour.
It is highly recommended to encourage production of jam using local raw
materials like (sugar beet) at household level and strict conditions must be
available while making jam at home.
Conclusions
References
References
http://www.wikipedia.info.
http://en.wikipedia.org/wiki/Pectin
AOAC. (2000). Association Of Official Analytical Chemists, Official Methods of Analysis
(17th Ed.). Arlington, VA. USA
Ruck J. A. (1969). Chemical Methods for Analysis of Fruits and Vegetable Products.
Canada Dept of Agric, Summerland B.C.: 14-33.
Pornsak S. (2003). Chemistry of Pectin and its Pharmaceutical Uses: A Review.
Silpakorn University international journal 3 (1–2): 206.
Joseph, Z. B. (1986). Sudan Food Composition Table, 2nd ed, National Chemical
Laboratories, Ministry of Health of Khartoum, Sudan.
THANK
YOU

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Savan

  • 2. Extraction of Pectin from Lemon and Orange Fruits Peels and Its Utilization in Jam Making. Prepared by Gohel Savan
  • 5. Oranges Oranges are a very good source of vitamins, especially vitamin C grew first in South and East Asia but now grows in many parts of the world. Orange is most commonly grown fruit in the world. From peels of Oranges “D-Limonene oil” & “Pectin” is extracted. Outer part of peel is source of “D-limonene Oil”. Inner Part of peel known as “Albedo” is source of Pectin.
  • 6. Lemon  Lemon (Citrus × limon ) is one of the important citrus fruits appreciably not only for its beautiful appearance and pleasing flavor but also for excellent food qualities.  the production area of lemon fruits in India is 258 (000'HA) while the production is 2978 (000'MT) (NHB database-2015- 16).  Lemon belongs to family Rutaceae. It is cultivated widely in the tropical and subtropical regions.  The origin of lemon fruit is Asia..
  • 7. Pectin  Pectin, in general, is a group of polysaccharides found in nature in the primary cell walls of all seed bearing plants and are invariably located in the middle lamella. “D-galacturonic acid”  Pectin Contains “neutral sugars” (xylose, galactose & arabinose ) which is present in Side Chains..  It was first isolated in 1825 by Henri Braconot. One of the richest sources of pectin is lemon or orange which contains about 30% of this polysaccharide. Pectin is naturally found in a number of plants : lemon peel, orange peel, apple pomace, carrots, sunflower-heads, guava, mangoes and papaya.
  • 8. Jam Jam is a product made by boiling fruit pulp with sufficient sugar to a reasonably thick consistency, firm enough to hold the fruit tissues in position . Lemon ,orange , Apple, pear, sapota (chiku),peach, papaya, carrot, straw- berry, raspberry, mango, tomato, grapes and muskmelon are used for preparation of jams. It can be prepared from one kind of fruit or from two or more kinds . Jam contains 0.5-0.6 per cent acid and sugar should not be more than 40 percent and TSS 68%.
  • 9. Typical levels of pectin in plants are: Apples - 1-1.5% Apricots - 1% Cherries - 0.5-3.5% Carrots approx. - 1.4% Citrus peels - 30%
  • 11. Extraction of Pectin Orange and lemon were peeled and dried for four days and powdered. Orange powder (500 g) and from lemon powder (500 g) were used. Then 5 liter distilled water and 50 ml HCL were added for each blend and then mixed and left for 24 hours, then filtered in separation device.  One liter of filtrate was added to 1 liter ethanol (95%), the mixture was put into centrifugation apparatus. Ten left one hour and filtered through Buchner funnel. The filtrate was washed with 250 ml acetone for drying and filtrate was dried at room temperature for 24 hours. The product was ground into fine powder and sieved by 40 mesh sieve to separate pectin from fiber. The pectin powder was then collected, weighed and packed in plastic container pending jam production.
  • 12. Flow Sheet For Extraction Orange & lemon Peel Filter Cake Ethanol Sample Mother Liquor Water Bath Precipitation of + Filtration Heating pectin Citric Acid DRY PECTIN Drying Jelly Pectin Centrifuge
  • 14. Few Indian Major Players are as under pectin Extraction  Krishna pectins pvt. Ltd. Devson Impex Private Limited Gujarat General Food Chem Pvt. Ltd. Asha Ram & Sons Private Limited
  • 15. Sr. no. Observation Quantity 1 Distilled Water 5 lit 2 Amount of Orange & lemon peels powder 500 gm + 500 gm 3 Distillate obtained(Oil) 20 ml 4 Acetone 250 ml Experimental Observations
  • 18. Table 1. Amounts and percentages of pectin extracted from orange and lemon Quantity ,gm Weight of pectin Percentage of pectin Lemon 500 gm 31.5g 15.25 % orange 500 gm 41.5g 20.75 %
  • 19. Table 2. Chemical composition of orange pectin jam; lemon pectin jam OPJ: orange pectin jam; LPJ: lemon pectin jam; CPJ: commercial pectin jam Parameters Extracted pectin jam Commercial pectin jam OPJ LPJ CPJ pH 4.20 3.50 3.90 TSS 60.0 61.0 57.0 Titrable acidity % 0.49 0.38 0.37 Moisture % 29.0 28.0 30.0 Ash % 0.36 0.35 0.32 Carbohydrate 62.0 66.02 61.83 Vitamin C (mg/100g) 35.0 51 22.0
  • 20. Table 3. Minerals contents of orange pectin jam , lemon pectin jam and commercial pectin jam OPJ: orange pectin jam; LPJ: lemon pectin jam; CPJ: commercial pectin jam Minerals OPJ LPJ CPJ Sodium mg/100g 188 160 88 Potassium mg/100g 148 153 86 Calcium mg/100g 35 24 17
  • 21. Table 4. Sensory evaluation scores* of orange pectin jam , lemon pectin jam commercial pectin jam Jam Colour & appearanc e texture flavour Overall acceptability OPJ 6.2b 6.8b 7.3b 7.1b LPJ 7.1b 6.5b 8.2a 6.9b CPJ 8.5a 8.2a 8.6a 8.4a
  • 22.
  • 24. The low gel strength of the jam can be improved by the addition of pectin during processing to attain the commercially acceptable gel strength or a combination of fruits rich in pectin can be used to make up for the deficiency. The combination with other fruits could serve to improve the flavour and the colour. It is highly recommended to encourage production of jam using local raw materials like (sugar beet) at household level and strict conditions must be available while making jam at home. Conclusions
  • 26. References http://www.wikipedia.info. http://en.wikipedia.org/wiki/Pectin AOAC. (2000). Association Of Official Analytical Chemists, Official Methods of Analysis (17th Ed.). Arlington, VA. USA Ruck J. A. (1969). Chemical Methods for Analysis of Fruits and Vegetable Products. Canada Dept of Agric, Summerland B.C.: 14-33. Pornsak S. (2003). Chemistry of Pectin and its Pharmaceutical Uses: A Review. Silpakorn University international journal 3 (1–2): 206. Joseph, Z. B. (1986). Sudan Food Composition Table, 2nd ed, National Chemical Laboratories, Ministry of Health of Khartoum, Sudan.