This document summarizes the results of stress tests conducted by EMODnet Sea-basin Checkpoints on various biological data themes. For species trend analysis in the Baltic Sea, data was often missing or not available across all sampling locations. Biomass data for eutrophication indicators in the Baltic was also summarized. Species stress tests in other regions found data availability and appropriateness to be partly adequate for some areas but with many gaps. Migration data from rivers was limited or not in a centralized database. Non-indigenous species data was most complete for the Baltic but fragmented in other regions. Fisheries landing data quality varied between countries but coastal and discard data availability was still limited for comprehensive stock assessments.