This study examined the effects of adolescent isoflurane exposure on subsequent ethanol intake behaviors in rats. The results showed that rats exposed to isoflurane as adolescents spent more time drinking both ethanol and sucrose solutions in a two-bottle choice test compared to controls, indicating altered appetitive reinforcement and reward pathways. However, isoflurane exposure did not increase ethanol preference relative to sucrose. This was likely due to increased sucrose consumption in isoflurane-exposed rats. The study provides preliminary evidence that adolescent anesthetic exposure may alter ethanol behaviors through neurobiological changes in reward pathways.