The Jagiellonian Center of Innovation provides a broad range of services in the field of protemic analysis based on electrophoretic, chromatographic, and mass spectrometric techniques. With this method, it is possible to separate complex mixtures of compounds and generate high-resolution mass spectra with mass measurement accuracy of 5 ppm (HRMS). The electrospray technique (ESI) is suitable for gentle ionization of studied molecules, while fragmentation in the collision chamber enables identification of unknown compounds. In the area of mass spectrometry, the Laboratory offers the following analyses: - Determination of molecular weight of proteins/peptides - Identification of proteins and peptides based on popular databases (UniProt, NCBI) - Identification and localization of post-translational modifications (PTMs) - Label-free protein quantification. The proteomic services listed above are complemented by protein characterization by electrophoretic, immunoenzymatic, and physicochemical methods. A wide array of techniques allows for the separation of proteins in complex mixtures, identification based on their molecular weight, physicochemical characterization, and analysis of a selected protein. The Laboratory also performs protein purification with the use of low pressure chromatography. We offer a service of isolation and analysis of proteins from complex mixtures (e.g. milk, body fluids). The laboratory has the ability to purify proteins from various sources. We conduct research and development projects in the field of isolation and biochemical analysis of proteins. A number of methods are available in the laboratory that allow for biochemical characterization of proteins: - Gel electrophoresis (SDS-PAGE, Native-PAGE) – Mini-PROTEAN Tetra Cell (Bio Rad) - Western Blot – Mini Trans-Blot® Cell (Bio Rad) - Multiangle light scattering (MALS) coupled with chromatographic system – DAWN8+ detector (WYATT) – protein molecular weight analysis in solution - UV-VIS spectroscopy – Evolution 201 (Thermo Scientific) - Microscale thermophoresis (MST) – Monolith NT.115 (Nanotemper) – - Analysis of protein interactions Surface plasmon resonance (SPR) – Biacore X-100 (GE Healthcare) – analysis of protein complex binding kinetics Enzyme immunoassay - (ELISA) – qualitative/quantitative determination of protein using immunoenzymatic assay The laboratory has experience in conducting research and development studies in the field of biopharmaceuticals. Our team prepares the results for scientific publications. We offer substantive and practical support for start-ups in proteomic research.

1. Laboratory of Chromatog
and Mass Spectrometry
BIOCHEMICAL ANALYSIS
OF PROTEINS

2. The Jagiellonian Center of Innovation has 5 modern
and well-equipped laboratories where it carries out
research for the following industries:
 pharmaceutical
 medical devices
 cosmetic
 grocery
 dietary supplements
 and for scientific universities
LABORATORIES

3. We provide analytical and consulting services within the
scope of qualitative and quantitative analyses of
compounds using liquid chromatography (HPLC,
UHPLC), gas chromatography (GC and GC-MS), and
supercritical state chromatography (CO2) (SFC).
We offer:
 Quantitative analyses of mixtures of liquid organic
compounds (GC, UHPLC)
 Purity analyses of organic compounds (GC, GC-
MS, UHPLC)
 Quality analyses of organic compounds (GC, GC-
MS)
LABORATORY OF
CHROMATOGRAPHY AND MASS
SPECTROMETRY

4. The Jagiellonian Center of Innovation
provides a broad range of services in the field
of proteomic analysis based on
electrophoretic, chromatographic, and mass
spectrometric techniques.
BIOCHEMICAL ANALYSIS
OF PROTEINS

5.  We work with FPLC chromatographic.
 We offer a service of optimisation of
protein purification from complex
mixtures.
 We use all available chromatographic
methods for protein preparation.
 We develop purification protocols in
context of research articles and
process optimisation.
 We also offer substantive and practical
support in proteomic research.
PROTEIN PURIFICATION

6. Mass spectrometry analysis using Impact II QTOF
(Bruker) we offer:
 Protein molecular mass determination with 5 ppm
accuracy (HRMS)
 Protein identification using standard databases (UniProt,
NCBI) or provided sequence
 Identification and localisation of post-translational
modifications (PTMs)
 Protein identification using top-down analysis
 Characterisation of drug-antibody conjugates (ADCs)
 Host-cell proteins contamination detection (HCPs)
 Quantitative analysis of proteins using label free strategy
MASS SPECTROMETRY

7. We use variety of techniques for preparation and
protein analysis:
 Basic techniques such as gel electrophoresis and
Western Blot
 Multi angle light scattering coupled with chromatographic
system – protein molecular weight determination – Wyatt
DAWN HELEOS 8+
 UV-VIS spectroscopy
PROTEIN CHARACTERISATION

8. The Laboratory of Chromatography and
Mass Spectrometry of the Jagiellonian
Center of Innovation has a range of
equipment dedicated to a variety of
analytical tests. The Laboratory’s liquid and
gas chromatographs coupled with various
detectors allow the analysis of a wide range
of chemical compounds: from identification
to quantitative tests.
We also have dedicated equipment for tests
in the area of mass spectrometry,
LABORATORY EQUIPMENT

9.  UV-Vis DAD (190–900 nm), ELSD
 Corona Veo RS (operating temperature range:
+5oC to +100oC)
 MALS (determination of mass from 100 Da to
1,250 kDa)
 DLS
 Q-ToF mass spectrometer (ionization sources:
ESI, APCI) (range: 20 to 15,000 m/z)
 Fluorescent FLD (range: Ex 200 to 630; Em 220
to 650)
 Refractometric (RI) detector
UHPLC (DIONEX)
COUPLED TO
DETECTORS

10.  Chromatography systems (Shimadzu)
with autosampler for liquid sample
injection and HeadSpace attachment
 Detectors: MS detector and flame
ionization detector (FID)
GAS CHROMATOGRAPHY

11.  Analytical chromatograph (Waters)
coupled to PDA detector
 Preparative chromatography system
coupled with PDA and MS (single
quadrupole) detector
SUPERCRITICAL CO2 FLUID
CHROMATOGRAPHY (SFC)

12.  High-resolution mass spectrometer – Impact II
QTOF (Bruker) (ionization sources: ESI, APCI)
(mas range: 20 to 50,000 m/z)
 Mastersizer 3000 (with Malvern Aero S; Hydro
EV; Hydro Sight units)
 Suppository/globule disintegration tester
(Erweka)
 Tablet disintegration time tester (Erweka)
 Tester for API release from solid dosage forms
coupled
to UV-Vis spectrophotometer (Erweka)
 Flow-through release tester (Erweka)
 Tester for API release from solid dosage forms
(Hanson)
 Friability/abrasion tester (Erweka)
OTHER EQUIPMENT

13. Contact:
Jagiellonian Center of Innovation Sp. z o.o. (LTD)
Life Science Park
Michała Bobrzyńskiego 14 St.
30 – 348 Cracow, POLAND
T: +48 12 297 46 00
F: +48 12 297 46 46
E: jci@jci.pl
www.jci.pl
www.cbkjci.pl