This document describes the synthesis and properties of phosphatidylhydroxyacetone (PHA), a new phospholipid formed by the fragmentation of cardiolipin under oxidative stress. Researchers first synthesized PHA from phosphatidylcholine using microbial phospholipase D, yielding about 80%. Analysis using NMR, IR, and thin-layer chromatography confirmed the identity and purity of PHA. PHA exhibits properties distinct from its precursor cardiolipin and may have new biological functions. Further study of PHA could provide insights into cardiolipin fragmentation processes in the body.