This is a poster presented at the American Association for Aerosol Research, Fall 2010 in Portland, OR. It details some of our work on expression profiling of pathogenic bioaerosols.
This e-book is an accompaniment to the book "Creative Workshop: 80 Challenges to Sharpen Your Design Skills," more details here: http://www.davidsherwin.com/creative
"Creative Workshop" contains 80 creative challenges that will help any designer reach a breadth of stronger design solutions, in various media, within any set time period. Exercises range from creating a typeface in an hour, to designing a paper robot in an afternoon, to designing web pages and other interactive experiences. Each exercise includes compelling visual solutions from other designers and background stories to help designers increase their capacity to innovate.
Before the book, however, there was a quarter-long class where design students had to complete 80 projects in just 11 weeks. This Teacher's Guide describes the pedagogical methods behind the book, how to create your own Creative Workshop class or workshop series, as well as how to utilize challenges from the book most effectively in a classroom setting. This text is intended for teachers of design and creative thinking, but it may also be helpful for designers and creative managers.
VDP & PSL -- all it can do to help grow your businessMarketingHighway
Jetletter PSL VDP Vision of Capabilities
The most comprehensive, feature rich VDP software available at a reasonable price. Your business WILL grow when you use PSL
Sony Computer Entertainment Inc. owns trademarks related to "Blu-ray Disc" and the "Blu-ray Disc" logo. Unauthorized copying, reproduction, rental, public performance, or broadcast of this game may violate applicable laws.
The annual report summarizes NCR Corporation's performance in 2002. It discusses revenue, operating income, cash flow, and year-over-year improvements. Key business segments like Teradata Data Warehousing, Financial Self Service, and Systemedia are highlighted. The report also covers NCR's global business model, goals to improve profitability through cost reductions, and opportunities for growth in 2003.
The document discusses how business is evolving and the need for individuals to evolve as well. It introduces ITIL, COBIT and ISO 20000 frameworks for IT service management best practices. The document also discusses process management and maturity levels to help organizations improve processes and services.
The document discusses blue whales and their conservation. It notes that blue whales were nearly hunted to extinction but their numbers are slowly increasing thanks to international bans on commercial whaling. The future of blue whales remains uncertain and dependent on environmental protections and cultural changes.
This article summarizes five creative ways that students at Burlington County High School asked their dates to the senior prom. The stories include:
1. A student followed a trail of popcorn balls to find a sign asking her to prom.
2. Another student opened her front door to find a friend delivering a pizza with the question "PROM?" spelled out in pepperoni.
3. A member of a band drew a picture asking a friend to prom in chalk on her driveway.
4. A student found a drawing of Snoopy inside a flipbook, with the question "PROM?" at the end.
5. A reality show anchor displayed a sign on his school's
The e-commerce market in Italy was worth over €14 billion in 2010. Leisure such as gaming accounted for nearly half of the market share at 48.5%. Tourism accounted for 31.4% while consumer electronics represented 7.1% of the market. The complete study is available at the provided website.
This e-book is an accompaniment to the book "Creative Workshop: 80 Challenges to Sharpen Your Design Skills," more details here: http://www.davidsherwin.com/creative
"Creative Workshop" contains 80 creative challenges that will help any designer reach a breadth of stronger design solutions, in various media, within any set time period. Exercises range from creating a typeface in an hour, to designing a paper robot in an afternoon, to designing web pages and other interactive experiences. Each exercise includes compelling visual solutions from other designers and background stories to help designers increase their capacity to innovate.
Before the book, however, there was a quarter-long class where design students had to complete 80 projects in just 11 weeks. This Teacher's Guide describes the pedagogical methods behind the book, how to create your own Creative Workshop class or workshop series, as well as how to utilize challenges from the book most effectively in a classroom setting. This text is intended for teachers of design and creative thinking, but it may also be helpful for designers and creative managers.
VDP & PSL -- all it can do to help grow your businessMarketingHighway
Jetletter PSL VDP Vision of Capabilities
The most comprehensive, feature rich VDP software available at a reasonable price. Your business WILL grow when you use PSL
Sony Computer Entertainment Inc. owns trademarks related to "Blu-ray Disc" and the "Blu-ray Disc" logo. Unauthorized copying, reproduction, rental, public performance, or broadcast of this game may violate applicable laws.
The annual report summarizes NCR Corporation's performance in 2002. It discusses revenue, operating income, cash flow, and year-over-year improvements. Key business segments like Teradata Data Warehousing, Financial Self Service, and Systemedia are highlighted. The report also covers NCR's global business model, goals to improve profitability through cost reductions, and opportunities for growth in 2003.
The document discusses how business is evolving and the need for individuals to evolve as well. It introduces ITIL, COBIT and ISO 20000 frameworks for IT service management best practices. The document also discusses process management and maturity levels to help organizations improve processes and services.
The document discusses blue whales and their conservation. It notes that blue whales were nearly hunted to extinction but their numbers are slowly increasing thanks to international bans on commercial whaling. The future of blue whales remains uncertain and dependent on environmental protections and cultural changes.
This article summarizes five creative ways that students at Burlington County High School asked their dates to the senior prom. The stories include:
1. A student followed a trail of popcorn balls to find a sign asking her to prom.
2. Another student opened her front door to find a friend delivering a pizza with the question "PROM?" spelled out in pepperoni.
3. A member of a band drew a picture asking a friend to prom in chalk on her driveway.
4. A student found a drawing of Snoopy inside a flipbook, with the question "PROM?" at the end.
5. A reality show anchor displayed a sign on his school's
The e-commerce market in Italy was worth over €14 billion in 2010. Leisure such as gaming accounted for nearly half of the market share at 48.5%. Tourism accounted for 31.4% while consumer electronics represented 7.1% of the market. The complete study is available at the provided website.
The document outlines an agenda for a DPS Training in 2009 with Melissa Holcombe, Sarah Markland, and Tony Nunn at Tasmanian Polytechnic and Sublime Studio. The training covers various topics related to creative thinking and design including images, color, space, tweaking, fonts, and finishing. Key concepts discussed include creative ability, process, research, analysis, thumbnails, typography, composition, and style.
This document discusses different types of community support, including proximity, sharing experiences, and modeling identity. It focuses on providing comfort through spiritual, emotional, intellectual, and practical support in a context of shared humanity, morality, and security.
The document provides instructions for three physics problems:
1) Calculate the resistance of a lamp when the current is 0.20 A.
2) Complete a table showing how voltage, current, and resistance change when adjusting a rheostat.
3) Calculate the current drawn and resistance of a fluorescent lamp rated at 15W, 120V.
Intalio is a privately held company that develops the leading open source business process management platform. It offers affordable and integrated BPM software through a commercial open source model. The software provides enterprise-grade capabilities like process automation, integration and monitoring. Intalio has over 500 customers worldwide and partners with systems integrators to help more organizations benefit from business process improvement.
The tendinous cords in the ventricles help close the atrioventricular valves when the ventricles contract. The cords connect the valve leaflets to the ventricle muscle walls and pull the leaflets closed as the ventricles contract, preventing backflow of blood into the atria.
The document provides a summary of the evaluation of EU public procurement directives. It finds that the directives are effective in increasing transparency and competition, resulting in estimated savings of 2.1-4% of contract values. However, cross-border procurement remains low and there are differences in implementation among member states. The directives are also found to be relevant, efficient and consistent with other EU policies, but with room for improvement regarding costs and unintended consequences. Overall, the directives are seen to provide EU added value by facilitating the single market, though greater coordination is still needed due to implementation differences.
Indian consumer trends are shifting as the population ages and income levels rise. Retirees are remaining active and pursuing entertainment options. Younger consumers are embracing new technologies and focused on health and security. As aspirations grow, consumers are drifting from tangible goods to prioritizing intangible experiences and leisure activities.
DesignS is a unified brand platform to promote Singapore design, consisting of a group partnership of design-centric institutes and associations in Singapore.
For updates, visit http://www.designs.com.sg/
The document summarizes interviews conducted as part of a project on conceptual models of procrastination. Three interviews were conducted where participants drew typical work patterns, mental models of procrastination, and answered 15 questions. The interviews resulted in 6 drawings and 3 transcripts. The team then synthesized the data individually by creating affinity diagrams for each interview. They then combined their individual models into two overall models, with one based on faces and diagrams and the other on sketched narratives.
The document appears to be from a magazine called YM008 discussing their latest issue and thanking readers for their continued support over the years. It promotes some of the feature stories in the issue including the cover story and asks readers to help spread the word about the magazine.
CSU ShortCourse Biomass And Bioenergy Jan 2008 Mpmmpattonmallory
This document discusses woody biomass utilization and sustainability. It provides an overview of forest resources and climate change, bioenergy production, biobased products, and ensuring sustainability. The key points are that biomass can help address climate change by displacing fossil fuels; various federal and state policies aim to promote biomass energy and products; and effective policy is needed to achieve sustainable use of forest resources for bioenergy and products while protecting environmental values.
Technology use and educational performanceFrancesc Pedró
1. Evidence shows technology use is connected to higher educational performance, but only when used at home, not in schools.
2. Socioeconomic status explains some of the variance in performance between countries regarding technology use.
3. Not all students approach technology use in the same way, and raising school use of technology may not necessarily improve performance if other factors are not addressed.
Wumart has several competitively distinct resources that form the basis of its competitive advantage regionally and domestically. These include its strategic relationships that provide access to prime retail locations, its early adoption of information technology, geographical advantages, its widely recognized brand image, and its strong corporate culture emphasizing respect, responsibility, innovation and attention to detail. Domestically, its control over its brand value and the difficulty for competitors to substitute its resources are also distinct competitive advantages.
This document is a psychrometric chart that shows temperature and humidity information at sea level under normal and low temperature conditions. It displays lines and scales for dry bulb temperature, wet bulb or saturation temperature, relative humidity, enthalpy, moisture content, and sensible heat factor.
The document provides information about a festival. It discusses wrapping up organization at the festival and discovering new experiences. The summary concludes by mentioning opening up the festival to the public.
This document outlines a job search plan with the following key steps:
1. Define goals for the type of job and company sought.
2. Let friends and professional contacts know about the job search.
3. Assess fit when considering opportunities by evaluating skills and interests.
This document discusses several plant growth processes regulated by hormones, including cell division promoted by auxins and transport, fruit ripening influenced by ethylene, and amylase production and bud inhibition related to gibberellins and cytokinins. It also references intercalary meristems, target cells, and deciduous plants.
Enterprise Collaboration: Can You Connect Social Learning and Business Perfor...Human Capital Media
How’s your social learning initiative going? If you are like most companies, you can easily match your situation with one of the following categories.
Category 1: We are just getting started and are unsure of how to proceed.
Category 2: We have started using social media for learning, but we don’t really have a strategy for how to leverage it.
Category 3: We have mastered the technology and its adoption and are seeing daily benefit.
If your situation falls into either Category 1 or Category 2, this session is a must for you. Learn how to put social learning into context and into action in your organization. If your situation is closest to Category 3, we encourage you to attend this session to share the successes of your experience with your peers. Either way, this session promises to connect you.
Eric Bruner, Chief Technologist, RWD Technologies
The document presents a model called the "Spiral of Knowledge" which depicts the evolution of knowledge over time. It shows knowledge progressing from the unknown to the known through research, recording, and application. The spiral shape represents how new insights build upon previous discoveries in an ongoing, cyclical process. In a few sentences, the document outlines the definition of management used in the General Enterprise Management approach.
Este documento trata sobre aeromicrobiología. Explica conceptos clave como bioaerosoles, que son partículas biológicas suspendidas en el aire. Describe los modos de transmisión respiratoria de los microorganismos, así como los procesos de lanzamiento, transporte y deposición de los bioaerosoles. También cubre factores que afectan la supervivencia microbiana en el aire, como la humedad y la temperatura. Por último, analiza aplicaciones de la aeromicrobiología y medidas de control de bioaerosoles
Fill volume as an indicator of surface heterogeneity in glass vials (expanded)matthewmicahhall
This presentation summarizes a recent study conducted at Alfred University that examined the corrosion of glass vials using a modified version of the hydrolytic resistance test. Vials made from converted glass tubing were filled with increasing incremental volumes of WFI and then autoclaved. The recovered liquid was analyzed for shifts in pH and the extractables were measured using ICP-OES. The results support the hypothesis that the heel region is a primary contributor to corrosion of glass vials made from converted tubing.
The document outlines an agenda for a DPS Training in 2009 with Melissa Holcombe, Sarah Markland, and Tony Nunn at Tasmanian Polytechnic and Sublime Studio. The training covers various topics related to creative thinking and design including images, color, space, tweaking, fonts, and finishing. Key concepts discussed include creative ability, process, research, analysis, thumbnails, typography, composition, and style.
This document discusses different types of community support, including proximity, sharing experiences, and modeling identity. It focuses on providing comfort through spiritual, emotional, intellectual, and practical support in a context of shared humanity, morality, and security.
The document provides instructions for three physics problems:
1) Calculate the resistance of a lamp when the current is 0.20 A.
2) Complete a table showing how voltage, current, and resistance change when adjusting a rheostat.
3) Calculate the current drawn and resistance of a fluorescent lamp rated at 15W, 120V.
Intalio is a privately held company that develops the leading open source business process management platform. It offers affordable and integrated BPM software through a commercial open source model. The software provides enterprise-grade capabilities like process automation, integration and monitoring. Intalio has over 500 customers worldwide and partners with systems integrators to help more organizations benefit from business process improvement.
The tendinous cords in the ventricles help close the atrioventricular valves when the ventricles contract. The cords connect the valve leaflets to the ventricle muscle walls and pull the leaflets closed as the ventricles contract, preventing backflow of blood into the atria.
The document provides a summary of the evaluation of EU public procurement directives. It finds that the directives are effective in increasing transparency and competition, resulting in estimated savings of 2.1-4% of contract values. However, cross-border procurement remains low and there are differences in implementation among member states. The directives are also found to be relevant, efficient and consistent with other EU policies, but with room for improvement regarding costs and unintended consequences. Overall, the directives are seen to provide EU added value by facilitating the single market, though greater coordination is still needed due to implementation differences.
Indian consumer trends are shifting as the population ages and income levels rise. Retirees are remaining active and pursuing entertainment options. Younger consumers are embracing new technologies and focused on health and security. As aspirations grow, consumers are drifting from tangible goods to prioritizing intangible experiences and leisure activities.
DesignS is a unified brand platform to promote Singapore design, consisting of a group partnership of design-centric institutes and associations in Singapore.
For updates, visit http://www.designs.com.sg/
The document summarizes interviews conducted as part of a project on conceptual models of procrastination. Three interviews were conducted where participants drew typical work patterns, mental models of procrastination, and answered 15 questions. The interviews resulted in 6 drawings and 3 transcripts. The team then synthesized the data individually by creating affinity diagrams for each interview. They then combined their individual models into two overall models, with one based on faces and diagrams and the other on sketched narratives.
The document appears to be from a magazine called YM008 discussing their latest issue and thanking readers for their continued support over the years. It promotes some of the feature stories in the issue including the cover story and asks readers to help spread the word about the magazine.
CSU ShortCourse Biomass And Bioenergy Jan 2008 Mpmmpattonmallory
This document discusses woody biomass utilization and sustainability. It provides an overview of forest resources and climate change, bioenergy production, biobased products, and ensuring sustainability. The key points are that biomass can help address climate change by displacing fossil fuels; various federal and state policies aim to promote biomass energy and products; and effective policy is needed to achieve sustainable use of forest resources for bioenergy and products while protecting environmental values.
Technology use and educational performanceFrancesc Pedró
1. Evidence shows technology use is connected to higher educational performance, but only when used at home, not in schools.
2. Socioeconomic status explains some of the variance in performance between countries regarding technology use.
3. Not all students approach technology use in the same way, and raising school use of technology may not necessarily improve performance if other factors are not addressed.
Wumart has several competitively distinct resources that form the basis of its competitive advantage regionally and domestically. These include its strategic relationships that provide access to prime retail locations, its early adoption of information technology, geographical advantages, its widely recognized brand image, and its strong corporate culture emphasizing respect, responsibility, innovation and attention to detail. Domestically, its control over its brand value and the difficulty for competitors to substitute its resources are also distinct competitive advantages.
This document is a psychrometric chart that shows temperature and humidity information at sea level under normal and low temperature conditions. It displays lines and scales for dry bulb temperature, wet bulb or saturation temperature, relative humidity, enthalpy, moisture content, and sensible heat factor.
The document provides information about a festival. It discusses wrapping up organization at the festival and discovering new experiences. The summary concludes by mentioning opening up the festival to the public.
This document outlines a job search plan with the following key steps:
1. Define goals for the type of job and company sought.
2. Let friends and professional contacts know about the job search.
3. Assess fit when considering opportunities by evaluating skills and interests.
This document discusses several plant growth processes regulated by hormones, including cell division promoted by auxins and transport, fruit ripening influenced by ethylene, and amylase production and bud inhibition related to gibberellins and cytokinins. It also references intercalary meristems, target cells, and deciduous plants.
Enterprise Collaboration: Can You Connect Social Learning and Business Perfor...Human Capital Media
How’s your social learning initiative going? If you are like most companies, you can easily match your situation with one of the following categories.
Category 1: We are just getting started and are unsure of how to proceed.
Category 2: We have started using social media for learning, but we don’t really have a strategy for how to leverage it.
Category 3: We have mastered the technology and its adoption and are seeing daily benefit.
If your situation falls into either Category 1 or Category 2, this session is a must for you. Learn how to put social learning into context and into action in your organization. If your situation is closest to Category 3, we encourage you to attend this session to share the successes of your experience with your peers. Either way, this session promises to connect you.
Eric Bruner, Chief Technologist, RWD Technologies
The document presents a model called the "Spiral of Knowledge" which depicts the evolution of knowledge over time. It shows knowledge progressing from the unknown to the known through research, recording, and application. The spiral shape represents how new insights build upon previous discoveries in an ongoing, cyclical process. In a few sentences, the document outlines the definition of management used in the General Enterprise Management approach.
Este documento trata sobre aeromicrobiología. Explica conceptos clave como bioaerosoles, que son partículas biológicas suspendidas en el aire. Describe los modos de transmisión respiratoria de los microorganismos, así como los procesos de lanzamiento, transporte y deposición de los bioaerosoles. También cubre factores que afectan la supervivencia microbiana en el aire, como la humedad y la temperatura. Por último, analiza aplicaciones de la aeromicrobiología y medidas de control de bioaerosoles
Fill volume as an indicator of surface heterogeneity in glass vials (expanded)matthewmicahhall
This presentation summarizes a recent study conducted at Alfred University that examined the corrosion of glass vials using a modified version of the hydrolytic resistance test. Vials made from converted glass tubing were filled with increasing incremental volumes of WFI and then autoclaved. The recovered liquid was analyzed for shifts in pH and the extractables were measured using ICP-OES. The results support the hypothesis that the heel region is a primary contributor to corrosion of glass vials made from converted tubing.
This document discusses microorganisms that can occur in air, including viruses, bacteria, and fungi. It describes how they can exist in air suspended in dust or liquid droplets. The document outlines the health risks of airborne microorganisms, including infectious diseases, allergies, and poisoning from toxins. Various types of airborne diseases caused by viruses and bacteria are also mentioned.
The document discusses various microorganisms found in air, including bacteria and viruses. It notes that microorganisms can exist up to 1000 feet above the Earth's surface attached to particles, and that dry weather leads to a higher microbial load in air while wet weather washes microorganisms out of the air. It then summarizes several pathogenic bacteria and viruses that are transmitted via the air or respiratory routes, such as Streptococcus, diphtheria, pertussis, tuberculosis, influenza, and their effects on human health.
facts of bacteria in air, microbes in air, bacteria in air, discharge of microbes in air, discharge of bacteria in air, sources of microbes in air, sources of bacteria in air
This document discusses the microbiology of air, including aero-microbiology, transmission of airborne microorganisms, common bacterial and fungal species found in indoor and outdoor air, and airborne diseases like tuberculosis, meningitis, influenza, and histoplasmosis. It also covers physical stresses on microorganisms in the air and methods to control microorganisms, such as ultraviolet radiation, chemical agents, filtration, and laminar airflow systems.
This document discusses personas and how they can be used to guide the design of social software and citizen media applications. It defines personas as fictional representations of target users that help focus the design process. It also discusses communicating personas to stakeholders and using scenarios, like Bridget Jones' experience on a train listening to her iPod, to bring personas to life. The document is from a presentation on personas and citizen media at the World Usability Day conference.
The document discusses best practices for managing requirements in software projects. It recommends managing requirements as deliverables rather than documents. Requirements deliverables have versioning and life cycles managed through a repository tool. This allows for automatic versioning, baselining, and communication across stakeholders. Change control begins with the first baseline to ensure building the right product. Managing requirements this way can help deliver projects on time, on budget and meeting objectives, even with changing requirements.
The document discusses a case study of the Nakambe sub-basin in Burkina Faso. It outlines the presentation which covers the basin system and sub-systems, an overview of the Nakambe basin, external drivers of change, impacts of basin changes on aquatic ecosystems and livelihoods, analytical tools used, and proposed management interventions. The basin has experienced changes like land use change, water storage, and impacts to hydrology and aquatic ecosystems. Analytical tools like mapping and modeling were used to assess these impacts. Management interventions focus on sustainable development and equitable benefit-sharing of small water bodies.
This document discusses biomass as a renewable energy source. It defines biomass as non-fossil organic resources from which energy can be produced, including vegetation, organic wastes, and photosynthetic organisms. Biomass can be burned directly for heat or used to create steam for electricity generation. It also describes various biomass feedstocks like energy crops, agricultural residues, and municipal solid waste. The document outlines the benefits of biomass but also notes barriers to its increased use like lack of experience with the technologies and high initial capital costs.
The poem expresses the speaker's deep love and connection to their partner. It says that if their love stops little by little, the speaker will also stop loving them little by little. However, if each day their partner feels destined for the speaker with sweetness, climbing flowers to their lips, the speaker's love will be repeated within them and will live in their partner's arms as long as they live.
The document summarizes a proposal for integrating Bangkok's mass transit system with its historic core districts to promote urban vitality and social cohesion. It begins by outlining the current fragmented conditions between the large-scale MTS and local urban fabric. The proposal then focuses on two districts located in an "urban dead void" that could potentially integrate with the historic core if redeveloped properly. Different types of streets and networks within the districts are analyzed. The vision is to use the MTS and underutilized streets to reconnect the districts, introducing mixed-use development and public transport options while preserving local heritage and networks. This would help balance development between the east and west sides of the river and address issues of segregation, sprawl
This document provides a list of pronouns in the 1st, 2nd, and 3rd person for both singular and plural forms in English. It includes subject and object pronouns as well as possessive determiners and pronouns.
ePortfolios: Documenting Life Long Learning of Professionals to Reflect Practice Excellence. Watson M. eHealth week 2010 (Barcelona: CCIB Convention Centre; 2010)
The document provides a summary of the author's professional experience in marketing communications. Over the past 15 years, the author has worked on a wide range of projects including advertising campaigns, website design, copywriting, and social media strategy. The author's experience spans both agency and in-house roles, and they are skilled in various digital marketing channels and platforms.
This document discusses Bureau Veritas' oil and gas services which focus on quality assurance and asset integrity management. It provides an overview of Bureau Veritas' technical centers network which brings engineering expertise to clients. It also highlights a client reference with Petrobras and Bureau Veritas' long-standing relationship providing QHSE management and innovative solutions. Risk-based inspection is discussed as a process to identify, assess, and map industrial risks. Fugitive emissions management is also summarized.
A survey asked respondents how confident they felt about using student response systems (SRS) in the classroom. The majority of respondents selected that they could know more about SRS or needed help, while a minority selected that they were very experienced or clueless about what an SRS is.
A survey asked respondents how confident they felt about using student response systems (SRS) in the classroom. The majority of respondents selected that they could know more about SRS or needed help, while a minority selected that they were very experienced or clueless about what an SRS is.
Green Packaging Asia provides green and sustainable packaging solutions. It has a team of designers and printers that work to create packaging with reduced environmental impact. The company aims to make the world more sustainable by partnering with customers on green packaging projects.
This document provides information about an upcoming personal branding training event including:
- Date: September 14, 2012 from 9am to 5pm
- Location: MDIS Dhoby Ghaut in Singapore
- Fee: $98 per person with discounts for members
- Topics covered: speaking with confidence, body language, authenticity and more
- Speakers include Jennifer Speake, Jose Wilkins, Vanessa Thomson and more
The document describes the implementation of Advanced Access scheduling at Triangle Family Practice. They were previously seeing 300 new patients per month with a 90 day wait for annual physicals. They decided to pilot Advanced Access to address high no-show rates, low patient and staff satisfaction, and inefficiencies. They closed to new patients and worked through the backlog. They changed policies to focus on same-day access and maximizing what is done at each visit. This improved access to providers, increased appointment acuity, and freed up future schedule space.
COH Online- The future of screening for distress in cancer settings (February11)Alex J Mitchell
This is a presentation I did at the us city of hope comprehensive cancer center in february 2011. The topic was future of screening for distress (and depression) in cancer; including an overview of recent screening findings.
Conversation Clusters: Grouping Conversation Through Human Computer DialogTony Bergstrom
The document discusses techniques for grouping conversations through human-computer dialog. It presents scenarios of group meetings where members discuss a design problem and different opinions are expressed. It notes challenges with speech recognition having 20-30% error rates and natural language processing being difficult. The document proposes leveraging human knowledge with computational methods to identify important moments in unstructured meeting transcripts.
Social media usage has increased dramatically globally, with over half of internet users engaging with social media platforms. Recruiters have traditionally relied on job boards for hiring, but social media provides new opportunities to source candidates, strengthen client relationships, and promote employer brands. Effective use of social media in recruitment can help increase candidate reach, assess company culture fit, and extend referral programs to a broader audience.
OpenID Foundation Japan Chapter AnnouncementDavid Recordon
The document discusses the growing adoption of OpenID as a single sign-on protocol for user authentication on the internet. It notes that over 10,000 websites now support OpenID login and that there are well over 250 million OpenIDs in use. OpenID allows users to log into multiple websites using a single digital identity and prevents websites from having access to user's login credentials. The OpenID Foundation was established to promote and develop OpenID as the framework for user-centric identity on the web.
1. Hi-Fidelity RNA Recovery for the Expression Profiling of
Airborne Bordetella pertussis Response to Atmospheric
Environmental Stress
KEVIN M. MCCABE (1), Jane Turner (1), Tracy L Nicholson (3), Tod Merkel (2), Mark Hernandez (1)
(1) Civil, Environmental and Architectural Engineering Department, University of Colorado, Boulder, Colorado
(2) Laboratory of Respiratory Pathogens, United States Food and Drug Administration, Bethesda, Maryland
(3) National Animal Disease Center, United States Department of Agriculture, Ames, Iowa
ABSTRACT:
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Gene expression patterns can provide a fundamental understanding of how
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airborne microbes respond to atmospheric environmental stresses. To
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obtain in-situ transcription signatures, we present a method that
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successfully isolates RNA from bacterial bioaerosol using calibrated liquid
impingers containing cold Trizol as the sole capture medium. This
approach preserves microbial RNA as it exists in its airborne state,
facilitating the analysis of a true “aerosol expression profile.” Microbial cell
walls are disrupted upon contact with Trizol, which rapidly denatures RNA
and associated proteins. This effectively stops transcription, prevents RNA
degradation, and provides a stable “snapshot” of relative mRNA levels near
the instant of collection. This approach eliminates any artifacts associated
with responses to the physical stresses microbes experience during their
capture in conventional liquids, or on agar and filter surfaces.
From a phosphate buffer containing fetal bovine serum, log phase
SUMMARY OF METHODS:
Bordetella pertussis was aerosolized with a 6-jet Collison nebulizer into a
•Grow 20 agar spread plates from a
well-mixed, 0.8 m3 chamber maintained at 22°C and 85% RH. Bioaerosols
single colony in stages
reached a density > 2 x 1010 microbes/m3, with a mean cell residence time
•Resuspend in 20mL PBS + 1% FBS
between 10 and 40 minutes. Microbes were simultaneously collected in 5
in BGI 6-jet Collison Nebulizer
swirling liquid impingers (SKC biosamplers) containing 20mL Trizol (4°C)
•Humidify chamber to 75% RH
for 40 minutes. Evaporative losses of the aqueous Trizol component
•Nebulize 10min (RH up to 80-85%)
requires volumetric reconstitution with RNAse free water prior to RNA
•Collect aerosol in SKC liquid impingers
extraction. Total RNA yields were well in excess of 3 micrograms - the
containing 20mL Trizol while continuing
minimum mass required for microarray expression analysis.
to nebulize for 30min.
•Monitor and adjust RH in chamber
In conclusion, the use of Trizol as an impinger medium facilitates the
•Stop Nebulizer; continue collection (10 min)
efficient collection and isolation of microbial RNA such that microarray
•Reconstitute Trizol with RNAse free water
expression analysis can be conducted with confidence on bioaerosols
•Extract RNA with standard Trizol protocol
maintained under controlled environmental conditions.
RESULTS: CONCLUSIONS:
Liquid impingers containing Trizol as the sole collection fluid can be used to •Hi-fidelity RNA collected from B. pertussis aerosol has successfully been
collect and “fix” bioaerosol RNA expression profiles with high enough RNA
utilized for expression microarrays, with comparisons to the Pre- and Post-
yields for microarray based gene expression profiling (red line).
aerosolization population in the nebulizer serving as respective controls.
•At the time of printing, these data were being analyzed to identify significant
expression changes in B. pertussis in response to aerosolization.
•Preliminary microarray results suggests a significant change in the
expression of numerous B. pertussis genes in response to aerosolization and
atmospheric environmental stress.
3μg Total RNA •Confirmation of these data by quantitative reverse transcriptase polymerase
chain reaction of specific gene transcripts will follow.