Payload Attachment Chemistry and Payload Design
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This document summarizes some benefits of using an immunogen attachment strategy for linking proteins to gold nanoparticles. The strategy allows for (1) an easy and mild single-step surface modification without chemicals that could damage proteins. (2) The linker length between the particle and protein can be adjusted. (3) Proteins do not need to be exposed to chemicals, preserving their integrity and activity. (4) Any protein with a His-tag can be attached using metal chelating, avoiding extra costs.
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Payload Attachment Chemistry and Payload Design
- 4. ...and some bonus from the immunogen attachment strategy (1) Very easy single step, mild and clean surface modification strategy (mix GNP and NTA-derivative, incubate and wash/spin) (2) Linker length can be adjusted by using different t-carboxyl-alkane-thiol or t-carboxyl-ethylenglycol-alkane-thiol (dicarboxymethyl-lysin + any molecule with carboxy and thiol can be easily obtained and utilized) (3) Proteins do not have to be exposed to any chemicals so that the integrity and activities of attachment target can be guaranteed (some proteins do not survive chemical modification step of attachment chemistry) (4) Use of general metal chelating strategy allows us to attach virtually any co-stimulatory molecules (proteins) with His-tag. (We use His-tag for protein purification anyway, so there is no extracost involved) (5) Potentially, purification/attachment on the GNP directly from expressed protein (Maybe good for the large scale production as long as protein is pure enough)