1. EpiTect Methyl qPCR System
Simple, Fast and Reliable DNA Methylation Analysis WITHOUT BISULFITE!
EpiTect® Methyl qPCR System
The Epitect Methyl qPCR System, using MethylScreenTM technology, is an innovative technology enabling fast and accurate detection of CpG island DNA methylation profiles of individual genes as
well as disease or pathway related gene panels. This technology
replaces the tedious & low-throughput bisulfite-based methods
with a simple restriction digest and quantitative PCR. The reliability and simplicity of the procedure make this technology an ideal
tool for profiling DNA methylation and biomarker development for
the study of stem cell growth and differentiation as well as cancer
and other human diseases.
How DNA Methylation PCR Arrays Works
1. DNA Digestion.
Input DNA +
Digestion Buffer
Mock
Simple, Fast and Reliable
No bisulfite conversion. Combine reliability of well-controlled
restriction digestions and quantitative PCR detection.
Relevance
Simultaneous detection of DNA methylation profiles of gene
panels relevant to specific cancer types.
Sensitive
Dependent
Double
Methylation sensitive
-
+
-
+
Methylation dependent
-
-
+
+
37 oC (6 hr - overnight)
2
2. Real-Time PCR.
Genome-Wide Coverage
We have PCR primers to detect the methylation status of all
your favorite genes.
Mock
Sensitive
Dependent
Double
Application
% Hypermethylated DNA
10%
20%
30%
40%
50%
60%
70%
>80%
Blood
MCF7
SKBR3
MDA-MB-231
Cell Lines
The heat map compares the hypermethylation status of 24 genes in the genomic
DNA of three breast cancer cell lines and blood genomic DNA as determined by
Human Breast Cancer Signature Panel EpiTect Methyl qPCR Arrays.
301.503.3187
fax:
Hypermethylated
Intermediately Methylated
Unmethylated
100
80
60
40
20
0
Gene 1
Gene 2
301.465.9859
Gene 3
Gene 4
Gene 5
Gene 6
EpiTect Methyl qPCR Array Principle
Mock
Unmethylated DNA
Real-Time PCR Signal
Epitect Methyl qPCR Arrays Validate Breast Cancer Gene
Methylation Status in Breast Cancer Cell Lines.
tel:
3. Data Analysis.
Total Input DNA [%]
MLH1
ATM
APC
CHFR
CAV1
CRBP1
FHIT
BRCA1
CDKN2A
CDKN1A
SFN
p14ARF
PYCARD
GADD45A
ZMYND10
GSTP1
CDH1
CALCA
MGMT
ABCB1
CDH13
WT1
p73
HIC1
<10%
24 Signature Breast Cancer Tumor Suppressor Genes
Breast Cancer Biomarker Validation
®
RT SYBR Green qPCR Mastermix
Detectable
Ct Value
Sensitive
No PCR
Signal
Hypermethylated DNA
Real-Time PCR Signal
Detectable
Ct Value
Detectable
Ct Value
Dependent
Detectable
Ct Value
No PCR
Signal
Double
No PCR
Signal
No PCR
Signal

2. EpiTect Methyl qPCR System
from The PCR Array Experts
Performance
Sensitivity Comparable to Bisulfite Sequencing
90
Percent of Total Input DNA [%]
Percent of Total Input DNA [%]
Results Comparable to Bisulfite Sequencing
100
Hypermethylated
Intermediately Methylated
Unmethylated
80
70
60
50
40
30
20
10
0
Bisulfite
Sequencing
MCF7
EpiTect
Methyl
Bisulfite
Sequencing
EpiTect
Methyl
SKBR3
Bisulfite
Sequencing
EpiTect
Methyl
100
90
Hypermethylated
Intermediately Methylated
Unmethylated
80
70
60
50
40
30
20
10
0
100
93.75
87.5
75
50
33.33
12.5
6.25
0
Percent SKBR3 Genomic DNA [%]
MB231
EpiTect Methyl qPCR Assays Yield Results Identical to
Bisulfite Sequencing. The methylation status of the cadherin
1 gene (CDH1) was determined using either bisulfite sequencing or
EpiTect Methyl qPCR Assays in three different breast cancer cell
lines known to have very different CDH1 methylation patterns.
EpiTect Methyl qPCR Assays Detect Hypermethylation
in Heterogeneous Samples Containing As Little As Five
Percent Tumor DNA. SKBR3 breast cancer cell line and normal
blood genomic DNA (encoding hypermethylated and unmethylated
HIC1, respectively) were mixed in different ratios. Using Human
HIC1 EpiTect Methyl qPCR Primers, the percentage of hypermethylated HIC1 relative to total promoter DNA in each mixture was
detectable even down to five percent.
EpiTect Methyl Products
Cancer-focused products
Pathway-focused products
Breast Cancer
Cancer miRNA
Colon Cancer
Epithelial to Mesenchymal Transition (EMT)
Gastric Cancer
Product
Leukemia and Lymphoma
Liver Cancer
Lung Cancer
Melanoma
Prostate Cancer
Tumor Suppressor Genes
Apoptosis
Cell Cycle
Cytokine Production
DNA Repair
Homeobox (HOX) Genes
Price
Pack Size
Inflammatory Response
Mental Disorders
Notch Signaling
Polycomb (PcG) Genes
Stem Cell Transcription Factors
Stress and Toxicity
T Cell Activation
T Helper Cell Differentiation
Tumor Suppressor Genes
Toll-like Receptor Signaling
TGF-β/BMP Signaling
Wnt Signaling
2 Free DNA Methylation PCR Arrays
Limited to 1 pack (2-plate) of free PCR
Arrays, per person, per lab. The purchase
of a DNA Methylation PCR Array Starter
Pack is required to qualify for the promotion. Offer expires December 31, 2011.
Catalog #
The EpiTect Methyl qPCR System uses the MethylScreenTM technology
provided under license from Orion Genomics, LLC.
©2011 QIAGEN, all rights reserved.
Trademarks: QIAGEN®, EpiTect®, DNeasy®, (QIAGEN Group); SYBR® (Molecular Probes, Inc.); MethylScreenTM (Orion Genomics, LLC.).
Request by calling: 888.503.3187 Use promotion code:
Please scan QR code or visit the link for promotion details:
SAB-Starter3
www.SABiosciences.com/MethylProfilerPilotStudy.php