Gregor Mendel conducted experiments with pea plants between 1856-1863. Through his experiments, he discovered two fundamental laws of inheritance: the Law of Segregation and the Law of Independent Assortment. The Law of Segregation states that alleles segregate and are passed to gametes independently. The Law of Independent Assortment states that different genes assort independently during gamete formation. Mendel's work laid the foundation for modern genetics although it was not widely recognized until the early 20th century.
This presentation is carrying all summary about the history of genetics that who discover genes which scientist work on it and there work summary of all these things is given here and it is very helpful for the students of genetics whether they are students of plant genetics or animals.
Concept of Sex chromosomes and autosomes,
Inheritance of X- linked genes – eye colour in Drosophila,
Inheritance of colour blindness in humans,
Inheritance of Y-linked Genes -Holandric genes in humans,
Sex influenced genes – baldness in humans
Sex-limited genes - feathering in domestic fowl
This presentation is carrying all summary about the history of genetics that who discover genes which scientist work on it and there work summary of all these things is given here and it is very helpful for the students of genetics whether they are students of plant genetics or animals.
Concept of Sex chromosomes and autosomes,
Inheritance of X- linked genes – eye colour in Drosophila,
Inheritance of colour blindness in humans,
Inheritance of Y-linked Genes -Holandric genes in humans,
Sex influenced genes – baldness in humans
Sex-limited genes - feathering in domestic fowl
Chapter 15: Chromosomal Basis of InheritanceAngel Vega
KEY CONCEPTS
15.1 Morgan showed that Mendelian inheritance has its physical
basis in the behavior of chromosomes: Scientific inquiry
15.2 Sex-linked genes exhibit unique patterns of inheritance
15.3 Linked genes tend to be inherited together because they are located near each other on the same chromosome
15.4 Alterations of chromosome number or structure cause
some genetic disorders
15.5 Some inheritance patterns are exceptions to standard
Mendelian inheritance
Examples of Codominance. The best example, in this case, is the codominance blood type. ABO group is considered to be a codominant blood group where both father’s and mother’s blood group is expressed. It means that the properties of the blood groups exist in the ABO type.
Codominance is a relationship between two versions of a gene. Individuals receive one version of a gene, called an allele, from each parent. If the alleles are different, the dominant allele usually will be expressed, while the effect of the other allele, called recessive, is masked.
Some references are coming from the internet, i just copied it.. credits to the owner. some information are not mine as well as the slide i just download it from the internet. My report in my Masters.
Here, Genetic disorder and chromosomal abnormality discussed briefly. *Types of the genetic disorder *briefly discussed on different genetic diseases *chromosomal anomaly i.e. structural and numerical anomaly. etc.
Discuss the methods Mendel utilized in his research that led to his success in understanding the process of inheritance
The science community ignored the paper, possibly because it was ahead of the ideas of heredity and variation accepted at the time. In the early 1900s, 3 plant biologists finally acknowledged Mendel’s work. Unfortunately, Mendel was not around to receive the recognition as he had died in 1884.
Chapter 15: Chromosomal Basis of InheritanceAngel Vega
KEY CONCEPTS
15.1 Morgan showed that Mendelian inheritance has its physical
basis in the behavior of chromosomes: Scientific inquiry
15.2 Sex-linked genes exhibit unique patterns of inheritance
15.3 Linked genes tend to be inherited together because they are located near each other on the same chromosome
15.4 Alterations of chromosome number or structure cause
some genetic disorders
15.5 Some inheritance patterns are exceptions to standard
Mendelian inheritance
Examples of Codominance. The best example, in this case, is the codominance blood type. ABO group is considered to be a codominant blood group where both father’s and mother’s blood group is expressed. It means that the properties of the blood groups exist in the ABO type.
Codominance is a relationship between two versions of a gene. Individuals receive one version of a gene, called an allele, from each parent. If the alleles are different, the dominant allele usually will be expressed, while the effect of the other allele, called recessive, is masked.
Some references are coming from the internet, i just copied it.. credits to the owner. some information are not mine as well as the slide i just download it from the internet. My report in my Masters.
Here, Genetic disorder and chromosomal abnormality discussed briefly. *Types of the genetic disorder *briefly discussed on different genetic diseases *chromosomal anomaly i.e. structural and numerical anomaly. etc.
Discuss the methods Mendel utilized in his research that led to his success in understanding the process of inheritance
The science community ignored the paper, possibly because it was ahead of the ideas of heredity and variation accepted at the time. In the early 1900s, 3 plant biologists finally acknowledged Mendel’s work. Unfortunately, Mendel was not around to receive the recognition as he had died in 1884.
Genetics- Chapter 5 - Principles of inheritance and variation.docxAjay Kumar Gautam
Genetics is a branch of biology concerned with the study of genes, genetic variation, and heredity in organisms. Though heredity had been observed for millennia, Gregor Mendel, Moravian scientist and Augustinian friar working in the 19th century in Brno, was the first to study genetics scientifically. Mendel studied "trait inheritance", patterns in the way traits are handed down from parents to offspring over time. He observed that organisms (pea plants) inherit traits by way of discrete "units of inheritance". This term, still used today, is a somewhat ambiguous definition of what is referred to as a gene.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
2. 2
A Deck of Genes
Blending hypothesis holds that the trait
that the children receive from their
parents is simply a mixture of the parental
characters
Predicts a uniform population of individuals
will result from a freely mating population,
over many generations (not seen!!!)
Why are some traits observed again after
skipping a generation?
3. -It was generally accepted that the
hereditary traits of the offspring of any
species were merely the diluted blending of
whatever traits were present in the
“parents”
-Similarly, it was also thought that, over
generations, a hybrid would revert to its
original form
-Implication being that a hybrid could not
create new forms
3
4. 4
A Deck of Genes
“Particulate” Hypothesis of Inheritance
the GENE idea
Offsprings receive heritable units (gene)
which retain their separate identities
An individual’s collection of genes is much
more like a deck of cards than a pail of
paint
Without getting diluted, genes can be
shuffled and passed along through
generations
5. 5
Mendel stated that physical
traits are inherited as
“particles”
Mendel did not know that the
“particles” were actually
Chromosomes & DNA
Developed decades before
chromosomes were observed
under the microscope and the
significance of their behavior
was understood
Particulate Inheritance
7. 1822 - born in Brunn, Austria, now, Brno,(Czech Repub.)
-received agricultural training in school along with basic
education
- as an adolescent, he overcame financial hardship and
illness to excel in high school and, later, at the Olmutz
Philosophical Institute
1843 – entered an Augustinian monastery
- considered becoming a teacher but failed the
necessary exam
1851 – left the monastery to pursue two years of study
in physics and chemistry Univ. of Vienna
- important for his development as a scientist
(under the mentorship of Doppler and Unger)
1857 – began breeding garden peas to study inheritance
7
8. 8
Gregor Johann Mendel
Developed the laws
of inheritance
Presented his findings
in the Natural History
Society of Brunn –
1865
Paper entitled,
“Experiments in Plant
Hybridization” – 1866
German language.
9. 9
Gregor Johann Mendel
Between 1856 and
1863, Mendel
cultivated and
tested some 28,000
pea plants
He found that the
plants' offspring
retained traits of
the parents
17. 17
Mendel's work was not recognized until the
turn of the 20th century
1900 - Carl Correns, Hugo deVries, and Erich
von Tschermak rediscovered and confirmed
Called the “Father of Genetics“
18. 18
Alleles - two forms of a gene (dominant
or recessive)
Dominant - stronger of two genes
expressed in the hybrid; represented by
a capital letter (R)
Recessive - gene that shows up less
often in a cross; represented by a
lowercase letter (r)
Genetic Terminologies
19. 19
Genetic Terminologies
Hybridization – mating or crossing of
two true-breeding varieties
True-breeding – varieties that that
produce only one character over many
generations
P generation – true-breeding parents or
parental generation
20. 20
Monohybrid cross - cross involving a
single trait
e.g. flower color
Dihybrid cross - cross involving two
traits
e.g. flower color & plant height
Genetic Terminologies
21. 21
Homozygous genotype – When the
two alleles are the same
(dominant or 2 recessive genes)
e.g. TT or tt; also called pure
Heterozygous genotype – When
the 2 alleles are different- one
dominant & one recessive allele
(e.g. Tt)
Genetic Terminologies
22. 22
F1 generation – first filial generation
parent (hybrid offspring)
F2 generation – second filial generation
parent (hybrid offspring)
Genotype - gene combination for a trait
(e.g. RR, Rr, rr)
Genetic Terminologies
23. 23
Phenotype - the physical feature
resulting from a genotype (e.g. red,
white); may also refer to physiological
traits that relate directly to appearance
(e.g. non self-pollination)
Hemizygous – there is only one allele
instead of two
Genetic Terminologies
26. 26
Mendel’s Experiment
Mendel chose to track only those characters
that occurred in two distinct, alternative
forms
Also made sure that he started with only
true-breeding varieties
He cross-pollinated two contrasting, true-
breeding pea varieties (P generation),
producing F1 generation offsprings
Allowing these F1 hybrids to self-pollinate
(or to cross-pollinate with other F1 hybrids)
produces an F2 generation offsprings
27. 27
Mendel’s Experiment
Had he stopped with F1 generation, the basic
patterns of inheritance would not have been
discovered
His quantitative analysis of the F2 plants
from thousands of genetic crosses allowed
him to deduce two fundamental principles of
heredity
Law of Segregation
Law of Independent Assortment
29. 29
Mendel reasoned that the heritable factor
for white flowers did not disappear in the
F1 plants, but was somehow hidden, or
masked, when the purple flower was present
In his jargon, purple flower color is a
dominant trait, and the white flower color is
a recessive trait
30. 30
According to him, the reappearance of the
white flower plants in the F2 generation was
evidence that the white flowers had not
been diluted or destroyed by coexisting
with the purple flower factor in the F1
hybrids.
Instead, it had been hidden in the presence
of the purple flower
He also observed the same pattern of
inheritance in six other characters, each
represented by six different traits
32. 32
First, alternative versions of genes account
for variations in inherited characters
* Alleles – can be related to the DNA and
chromosomes
Mendel’s Model
33. 33
Second, for each
character, an organism
inherits two copies
(two alleles) of a gene,
one from each parent
* made this
assertion despite not
knowing about the
role, and even
existence of the
chromosomes
Mendel’s Model
34. 34
Third, if the two
alleles at a locus
differ, then one, the
dominant allele,
determines the
organism’s
appearance; the
other, the recessive
allele, has no
noticeable effect on
the organism’s
appearance
Mendel’s Model
35. 35
Fourth, is the law of segregation which holds that
the two alleles for a heritable character segregate
(separate from each other) during gamete
formation and end up in different gametes
* thus, an egg or a sperm gets only one of
the two alleles that are present in the
somatic cells of the organism making the
gamete
* in terms of chromosomes, this
segregation corresponds to the distribution
of the two members of a pair of homologous
chromosomes to different gametes in meiosis
Mendel’s Model
38. 38
Mendel’s First Three Postulates
1. UNIT FACTORS IN PAIRS
Genetic characters are controlled by unit
factors existing in pairs in individual organisms.
In the monohybrid cross involving tall and dwarf
stems, a specific unit factor exists for each
trait
Each diploid individual receives one factor from
each parent.
Because the factors occur in pairs, 3 possible
combinations are possible: 2 factors for tall
stems, or 1 of each factor
Every individual possesses one of 3 combinations,
which determines stem height.
39. 39
2. DOMINANCE/RECESSIVENESS
When 2 unlike unit factors responsible for a
single character are present in a single
individual, one 1 unit factor is dominant to the
other, which is recessive.
The trait not expressed is controlled by the
recessive unit factor.
The terms dominant and recessive are also used
to designate traits.
Tall stems are said to be dominant over
recessive dwarf stems.
40. 40
3. SEGREGATION
During the formation of gametes, the paired
units separate, or segregate, randomly so that
each gamete receives one or the other with
equal frequency.
If an individual contains a pair of unit factors
(e.g., both specific for tall), then all the
gametes receive one of that same kind of unit
factor (tall).
If an individual contains unlike unit factors
(e.g., one for tall and one for dwarf), then
each gamete has a 50% probability of receiving
either the tall or dwarf unit factor.
41. 41
The postulates provide a suitable explanation
for the results of the monohybrid crosses
He reasoned that P1 tall plants contained
identical paired unit factors, as did the P1
dwarf plants.
The gametes of tall plants all receive 1 tall unit
factor (segregation) and so is the case with the
dwarf plants
P1 x
Gametes x
DD dd
D d
42. 42
Following fertilization, all F1 plants receive one
unit factor from each parent (tall factor from
one and a dwarf factor from the other),
reestablishing the paired relationship.
But because tall is dominant to dwarf, all F1
plants are tall
P1: x
Gametes x
F1:
DD dd
D d
Dd
43. 43
When F1 plants form gametes, the postulate of
random segregation demands that each gamete
receives either the tall or the dwarf unit
factor.
Following fertilization during F1 selfing, four F2
combinations will result with equal frequency:
1. 1 tall/tall
2. 1 tall/dwarf
3. 1 dwarf/tall
4. 1 dwarf/dwarf
Therefore, the F2 is predicted to have ¾ tall and
¼ dwarf, or a ratio of 3:1
44. 44
4. INDEPENDENT ASSORTMENT
Two or more genes assort independently, i.e.,
each pair of alleles segregates independently of
each other pair of alleles, during gamete
formation.
Applies only to genes (allele pairs) located on
different chromosomes (non-homologous), or to
genes that are very far apart on the same
chromosome.
45. Sample Problems
1. Possible colors in cat are black and white, where white is
dominant. List the possible genotypes.
BB/Bb: White bb: black
2. List the possible genoytpes and phenotypes in rose color
flower where red color is dominant over white.
Genotype: WW/Wh: red ww: white
Phenotype: red and white
3. Classify the following as to homozygous and heterozygous.
a. bb: homo- c. Hh: hetero-
b. Rr: hetero- d. GG: homo-
4. Give the genotype for the following using T and t as the
alleles.
a. homozygous recessive: tt c. heterozygous:Tt
b. homozygous dominant:TT
45
47. 47
Test cross –
when an organism
of a known
dominant
phenotype but
unknown genotype
is crossed with a
homozygous
recessive
individual;
to determine
what alleles are
present in the
genotype
48. 48
Back cross - cross between F1 hybrid with
any of its parents or genetically similar to
its parent;
to achieve offspring with a genetic identity
which is closer to that of the parent
49. 49
Reciprocal cross – cross that involves the
reversal of the sex of the parents
to test the role of parental sex on
inheritance pattern
Out cross – cross when F1 progeny is
crossed with dominant parents;
to increase genetic diversity, thus, reducing
the probability of an individual being subject
to disease or genetic abnormalities
50. 50
Test cross:
P: _ _ x yy
F1: Yellow offsprings
YY x yy
All Yy
(All Yellow)
y y
Y Yy Yy
Y Yy Yy
Yy x yy
½ Yy
½ yy
y y
Y Yy Yy
y yy yy
YY
Yellow
Yy
Using pea plants, a plant with an unknown dominant
genotype yielded yellow offspring. Determine the
genotype of the unknown parent.
51. 51
Back cross:
P: TT x tt
F1: Tt Tall
Tt x TT
½ TT All Tall
½ Tt
T T
T TT TT
t Tt Tt
Tt x Tt
¼ TT ¾ Tall
½ Tt
¼ tt ¼ short
T t
T TT Tt
t Tt tt
52. 52
A B
AA BB
A A B B
AB
A A B B
B B
A AB AB
A AB AB
B B
A AB AB
A AB AB
Reciprocal
Cross
54. 54
Monohybrids - Individuals produced from a
cross of true-breeding parents
meaning, they are heterozygotes for the
trait in question
Monohybrid Cross - cross between
heterozygotes
cross between two organisms involving a
single character
56. 56
Monohybrid Cross
Heterozygous dominant x heterozygous
dominant
Genotypic ratio = 1 hd:2 het d:1recessive
Phenotypic raio = 3 dominant phenotype: 1
recessive phenotype
57. 57
Punnett Square
Each of the possible gametes is assigned a
column or a row:
The vertical column represents those of the
female parent, and the horizontal represent
those of the male parent
After assigning the gametes to the rows and
columns, the new generation is predicted by
entering the male and female gametic
information into each box thus, predicting every
possible resulting genotype
By filling out the the Punnett square, all
possible random fertilization events are listed.
60. Sample Problems
1. In dogs, wire hair (S) is dominant to smooth (s). In a cross of a
homozygous wire-haired dog with a smooth-haired dog, what would be
the phenotypes and genotypes of the offspring.
P: SS x ss
G: x
F1: ?
Genotypic ratio: All Ss
Phenotypic ratio: All wire-haired
60
S S
s Ss
Wire-haired
Ss
Wire-haired
s Ss
Wire-haired
Ss
Wire-haired
S s
61. Sample Problems
2. Wood rats are medium-sized rodents with lots of interesting
behaviors. Assuming that the trait of bringing home shiny objects (H) is
dominant to the trait of carrying home dull objects (h). Suppose 2
heterozygous individuals are crossed, what would be the genotypic and
phenotypic ratios?
Genotypic ratio: ¼ DD; 2/4 Dd; ¼ dd (1:2:1)
Phenotypic ratio: ¾ Shiny objects: ¼ Dull objects (3:1)
61
D d
D DD Dd
d Dd dd
62. Sample Problems
3. Saguaro cacti are very tall cylindrical plant with two arms, one on each
side. You have the same species at home where one arm is longer than the
other. Assume that arm length is controlled by a single gene with arms of
the same length (A) being dominant to arms of the different lengths (a),
a. what is the genotype of your cactus?
b. If your cactus fertilizes one that is heterozygous
for arms of the same length, give the genotype of the
offspring.
GR: ½ Vv: ½ vv
PR: ½ same length :
½ different length
62
V v
v Vv vv
v Vv vv
63. Sample Problems
4. Suppose that long tails (L) in a species of blackbird were dominant to
short tails (l). A female short-tailed blackbird mates with a long-tailed
individual which had one true-breeding parent with a long tail and one
true-breeding parent with a short tail.
a. what is the genotype of the male blackbird? Ss
b. what is the genotype of their offspring? Ss : ss (1:1)
c. what is the phenotype of their offspring? 1 long tail :
1 short tail
63
s s
S Ss Ss
s ss ss
64. Sample Problems
5. Tongue-rolling ability is dominant to not being able to roll
the tongue. Suppose that a heterozygous tongue-roller
marries and mates somebody who cannot role his tongue.
What will be the genotypic and phenotypic ratios of the
offspring?
GR- Nn : nn (1:1)
PR- 1 tongue roller : 1
non-tongue roller
64
n n
N Nn Nn
n nn nn
65. 65
Example:
6. In cats, black fur color is dominant. Two
heterozygous cats with black fur mate
together. Show the Punnett square.
a. what is the probability that they will
produce a cat with black fur?
b. what is the probability that the baby cat
will be homozygous?
c. calculate the phenotypic and genotypic
ratios
67. 67
Example:
In cats, black fur color is dominant. Two
heterozygous cats with black fur mate
together. Show the Punnett square.
a. what is the probability that they will
produce a cat with black fur?
Black fur = ¾ = 75%
68. 68
Example:
In cats, black fur color is dominant. Two
heterozygous cats with black fur mate
together. Show the Punnett square.
b. what is the probability that the baby cat
will be homozygous?
Probability that baby is homozygous = 2/4
or 50%
69. 69
Example:
In cats, black fur color is dominant. Two
heterozygous cats with black fur mate
together. Show the Punnett square.
c. calculate the phenotypic and genotypic
ratios
Phenotypic ratio: 3 black fur : 1 brown fur
Genotypic ratio: 1:2:1 (1BB:2Bb:1bb)
70. 70
Example:
7. A homozygous wolf with blue eyes
mates with a heterozygous wolf with
brown eyes. Brown being dominant
to blue. Use a Punnet square.
a. what is the probability that they
will produce a wolf with blue eyes?
b. calculate the phenotypic and
genotypic ratios.
72. 72
a. P = 2/4 or 50%
b. Phenotype ratio:
Brown : Blue
2 : 2
1 : 1
Genotype ratio:
Bb : bb
2 : 2
1 : 1
73. 73
P1 Monohybrid Cross Review
Homozygous dominant x Homozygous
recessive
Offspring all Heterozygous
(hybrids)
Offspring called F1 generation
Genotypic & Phenotypic ratio is ALL
ALIKE
74. 74
F1 Monohybrid Cross Review
Heterozygous x heterozygous
Offspring:
25% Homozygous dominant RR
50% Heterozygous Rr
25% Homozygous Recessive rr
Offspring called F2 generation
Genotypic ratio is 1:2:1
Phenotypic Ratio is 3:1
77. 77
As a natural extension of the monohybrid cross,
Mendel also designed experiments in which he
examined two characters simultaneously
(dihybrid cross, or a two-factor cross).
Law of Independent Assortment
78. 78
Using the result of his dihybrid experiments,
Mendel developed what is now the law of
independent assortment
* two or more genes assort independently, i. e.,
during gamete formation, each pair of alleles
segregates independently of each other pair
of alleles
This law applies only to genes (allele pairs) located
on different chromosomes (e. i., on non-
homologous chromosomes), or, alternatively, to
genes that are very far apart on the same
chromosome
Law of Independent Assortment
79. 79
Alleles for different traits are distributed to
sex cells (& offspring) independently of one
another.
* Genes are packaged into gametes in all
possible allelic combinations, as long as each
gamete has one allele for each gene.
This law can be illustrated using dihybrid
crosses.
Law of Independent Assortment
80. 80
If the hybrids must
transmit their alleles in
the same
combinations in which
the alleles were
inherited from the P
generation, then the
F1 hybrids will
produce only two
classes of gametes:
YR and yr (dependent
assortment)
81. 81
If the two pairs of
alleles segregate
independently of
each other, (genes
are packaged into
gametes in all
possible allelic
combinations, as
long as each gamete
has one allele for
each gene, F1 plant
will produce four
classes of gametes in
equal quantities: YR,
Yr, yR, and yr
(independent
assortment)
88. 88
Dihybrid Cross
Traits: Seed shape & Seed color
Alleles: W round
w wrinkled
G yellow
g green
WwGg x WwGg
WG Wg wG wg
All possible gamete combinations
FOIL
WG Wg wG wg
90. 90
Dihybrid Cross
Round/Yellow: 9
Round/green: 3
wrinkled/Yellow: 3
wrinkled/green: 1
9:3:3:1 phenotypic
ratio
WG Wg wG wg
WG
Wg
wG
wg
WWGG WWGg WwGG WwGg
WWGg WWgg WwGg Wwgg
WwGG WwGg wwGG wwGg
WwGg Wwgg wwGg wwgg
91. 91
Question:
How many gametes will be produced
for the following allele arrangements?
Note: 2n (n = # of heterozygotes)
1. RrYy
2. AaBbCCDd
3. MmNnOoPPQQRrssTtQq
92. 92
Answer:
1. RrYy: 2n = 22 = 4 gametes
RY Ry rY ry
2. AaBbCCDd: 2n = 23 = 8 gametes
ABCD ABCd AbCD AbCd
aBCD aBCd abCD abCD
3. MmNnOoPPQQRrssTtQq: 2n = 26 = 64
gametes
94. 94
Dihybrid Cross
Purple/Round: 9
Round/wrinkled: 3
white/Round: 3
white/wrinkled: 1
9:3:3:1 phenotypic
ratio
PI Pi pI pi
PI
Pi
pI
pi
PPRR PPRi PpRR PpRr
PPRr PPrr PpRr Pprr
PpRR PpRr ppRR ppRr
PpRr Pprr ppRr pprr
95. Exercises
1. Flower color & Pod shape
2. Stem length & Flower position
3. Seed color & Pod color
4. Pod shape & Stem length
5. Flower position & Seed color
6. Seed shape & Pod shape
7. Pod shape & Pod color
8. Flower color & Stem length
9. Flower position & Pod color
10. Seed shape & Flower color
95
96. 96
Summary of Mendel’s laws
LAW
PARENT
CROSS
OFFSPRING
DOMINANCE DD x dd
tall x short
100%
tall
SEGREGATION
Dd x Dd
tall x tall
75% tall
25% short
INDEPENDENT
ASSORTMENT
WwGg x WwGg
round & green
x
round & green
9/16 round seeds & green
pods
3/16 round seeds & yellow
pods
3/16 wrinkled seeds & green
pods
1/16 wrinkled seeds & yellow
pods
97. Summary of Mendel’s Hypothesis
1. Genes can have alternate versions called
alleles.
2. Each offspring inherits two alleles, one from
each parent.
3. If the two alleles differ, the dominant allele is
expressed. The recessive allele remains
masked unless the dominant allele is absent.
4. The two alleles for each trait separate during
gamete formation. This now called: Mendel's
Law of Segregation
97
98. 98
◊ The elegance of mendel’s experiments
was partly due to the complete
consistency between his observation and
hypotheses he developed.
◊ However, after Mendel’s work was
rediscovered, it became clear that
simple Medelian model did not
adequately predict experimental
observations in all situations.
99. 99
Dihybrid Cross
TtRr X TtRr
Each parent can produce 4 types of
gametes.
TR, Tr, tR, tr
Cross is a 4 X 4 with 16 possible
offspring.
100. 100
RESULTS
9 Tall, Red flower
3 Tall, white flower
3 short, Red flower
1 short, white flower
Or: 9:3:3:1