A detailed presentation on very widely known genus of nematode MELOIDOGYNE.

2. MELOIDOGYNE JAVANICA
PRESENTED TO -
• PROF. SOBITA SIMON
• PROF. ABHILASHA B. LALL
PRESNTED BY -
• KRITI
B. SC. [HONS] HORTICULTURE
18BSHORH041

3. FIG. MELOIDOGYNE JAVANICA

4. TABLE OF CONTENT• TAXONOMY
• HOSTS
• SYMPTOMS
• DIAGNOSIS
Morphological characters
Biochemical
Molecular
• MANAGEMENT
Biochemical
Chemical
Cultural practices
• PATHOGENESIS
• INFECTED PLANTS
• REFRENCES

5. TAXONOMY
• KINGDOM : Animalia
• PHYLUM : Nematoda
• CLASS : Secementea
• ORDER : Tylenchida
• SUPER FAMILY : Tylenchoidea
• FAMILY : Hetroderidae
• GENUS : Meloidogyne
• SPECIES : M. javanica

6. HOSTS
• Meloidogyne javanica is a nematode pathogen which infects over 770
species of plants.
• The host of these pathogen includes crops of highly economic value.
• The host range includes tea, grapevine, fruit tree, cerals, ornamentals,
bell pepper, capsicum and ornamentals.
• Meloidogyne javanica is also considered as pathogen of agricultural
importance because they severly affect the major agricultural crops.
• It affects crops on worldwise basis.

7. SYMPTOMS
• Abnormal leaf colour
• Abnormal leaf form
• Wilting leaf galls
• Swollen roots
• Reduced root system
• Dwarfing
• Senesence

8. SYMPTOMS

9. DIAGNOSIS
• MORPHOLOGICAL DIAGNOSIS
• Head shape and stylet morphology of males are useful characteristics in the
identification of M. javanica.
• When specimens are placed in lateral position, the distance between the dorsal
esophageal gland orifice to the stylet base can be used to distinguish between
species of Meloidogyne.
• The shape of the perineal region, dorsal arch, dorsal striae, lateral lines and
phasmids are all useful characters in identification.

10. DIAGNOSIS
• BIOCHEMICAL DIAGNOSIS
• Biochemical diagnostics methods are used when diagnosing nematode diseases.
• One technique frequently utilized is isoenzyme phenotyping.
• This analysis is based on the mobility of enzymes in the extracted protein, which is
diagnostic of different species of Meloidogyne.

11. DIAGNOSIS
• MOLECUAR DIAGNOSIS
• There are many molecular techniques that are becoming increasingly more
common, as they are easy and quick and cheap.
• Species – specific PCR is commonly utilized ,which uses species specific primers to
target certain nematodes based on SCAR [ Sequence – Characterized amplified
region ].

12. MANAGMENT
• BIOCONTROL MANAGEMENT
• One management strategy is being used to control M. javanica is a plant growth –
promoting bacteria. This biocontrol is specifically utilized in tomatoes and bell
pepper, where fluorescent pseudomonas produce an antibiotic 2, 4 – D.
• Another biocontrol with proven success in controlling Meloidogyne javanica is the
fungus Tricoderma harzianum. The fungus is able to infect nematode eggs and
juveniles and destroy them, consequently decreasing nematode infection.

13. MANAGMENT
• CHEMICAL MANAGEMENT
• Chemical methods is used less due to the toxicity and contamination potential.
• The most effective nematicides used against this species is Rugby in a dosage of 8
ppm .
• A more recent development in the management of Meloidogyne javanica is seed
treatment
• Treating seeds with abamectin before planting to has been proven effective against
M. javanica.

14. MANAGMENT
• CULTURAL PRACTICES
• A cultural practice used to control Meloidogyne javanica is crop rotation with non –
host species or resistant cultivars.
• Rotation crops such as marigold, perennial grasses and Bermuda grass have been
successful in suppressing the disease caused by M. javanica.
• This management style is effective when the nematodes are host – specific, as
rotating with a non – host crop eliminates the pathogen’s ability to infect.

15. PATHOGENESIS

16. PATHOGENESIS
FIG. MELOIDOGYNE

17. INFECTED PLANTS
• Wheat
• Potatoes
• Bell pepper
• Capsicum
• Tea
• Grapevine
• Ornamental flowers

18. REFRENCES
www.wikipedia.nic.in
www.researchgateonline.com
www.ncbi.in
www.agris.fao.nic