This document discusses methods for determining cell viability. It defines viability as the capacity for replication over a given timeframe. Methods for counting viable cells include indirect dilution-based techniques where colonies are counted after culturing, and direct techniques like nalidixic acid treatment, fluorogenic dyes, and microradiography that identify viable cells without culturing. A variety of assays can also assess properties of viable cells like integrity, permeability, enzyme content, and energy status to evaluate effects on cell viability.
The MTT assay and the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color The main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays)
It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth
This document describes the MTT assay, a colorimetric assay used to measure cell viability and cytotoxicity. The MTT assay works by using the enzyme mitochondrial dehydrogenase in living cells to reduce the yellow tetrazolium dye MTT to purple insoluble formazan. The amount of formazan produced is directly proportional to the number of viable cells. The document outlines the principle, reagents, procedure, troubleshooting, advantages, and disadvantages of the MTT assay. Commonly available MTT assay kits are also listed.
This document discusses methods for determining cell viability. It defines viability as the capacity for replication over a given timeframe. Methods for counting viable cells include indirect dilution-based techniques where colonies are counted after culturing, and direct techniques like nalidixic acid treatment, fluorogenic dyes, and microradiography that identify viable cells without culturing. A variety of assays can also assess properties of viable cells like integrity, permeability, enzyme content, and energy status to evaluate effects on cell viability.
The MTT assay and the MTS assay are colorimetric assays for measuring the activity of enzymes that reduce MTT or close dyes (XTT, MTS, WSTs) to formazan dyes, giving a purple color The main application allows to assess the viability (cell counting) and the proliferation of cells (cell culture assays)
It can also be used to determine cytotoxicity of potential medicinal agents and toxic materials, since those agents would stimulate or inhibit cell viability and growth
This document describes the MTT assay, a colorimetric assay used to measure cell viability and cytotoxicity. The MTT assay works by using the enzyme mitochondrial dehydrogenase in living cells to reduce the yellow tetrazolium dye MTT to purple insoluble formazan. The amount of formazan produced is directly proportional to the number of viable cells. The document outlines the principle, reagents, procedure, troubleshooting, advantages, and disadvantages of the MTT assay. Commonly available MTT assay kits are also listed.
1. Nowoczesne metody utrzymania hemostazy w kardiochirurgii Maciej Brzeziński Klinika Kardiochirurgii i Chirurgii Naczyniowej Gdański Uniwersytet Medyczny