Mathew Nordland is a senior at Purdue University studying biochemistry. He has worked as a research assistant since 2015 under Dr. Jody Banks investigating epigenetic gene silencing using techniques like PCR, qPCR, and CRISPR/Cas9. His research has focused on cloning RNAi constructs, assessing differential gene expression, and elucidating the chemical structure of a pheromone in the Ceratopteris sex determination pathway. Upon graduation in 2018, he plans to co-author two publications on his research regarding RNAi-mediated gene silencing and the structural elucidation of a target pheromone.

1. MATHEW NORDLAND
330 North GrantStreet, Lafayette,IN 47906 | 765-413-6199| mnordlan@purdue.edu
EDUCATION
Purdue University,WestLafayette, IN
BachelorsofSciencein Biochemistry 2018
GPA: 3.95
RESEARCH EXPERIENCE
Principle Investigator: Dr. Jody Banks,PurdueUniversity
Research Assistant June, 2015-Present
 Cloned RNAi constructs for epigeneticgeneticsilencing utilizing PCR, restriction digestion, ligation, and transformation techniques
 Assessed differential geneexpressionusing comparative ΔΔCT qPCR,RTPCR
 Administered biolisticbombardmentto integrateRNAi constructs into gametophytes via gold particle carriers
 EngineeredCRISPRCAS9constructs for epigeneticgenesilencing.
 Isolated ACEpheromoneusing XADhydrophobic resin, solvent partitioning and High PressureLiquid Chromatography
 ElucidatedACE ChemicalStructurevia Mass Spectrometryand Nuclear MagneticResonance
 Prepared plant tissuecultures for stable andtransient transformation ofconstructs
 Utilized fluorescence microscopy for photographs ofgametophytes
 Mediated greenhouse containing Ceratopteris, Pteris, andSelaginella
 Awarded IndependentFunding through Purdue ARPundergraduate research grant
 Advised PCR andnucleicacidisolation toundergraduate planttaxonomyclasses
FUTURE PUBLICATIONS
Estimated Publication–December2016
Co-Author
RNAi mediatedgenesilencing ofgenes intheCeratopteris sexdetermination pathway producing a
sex change phenotype
Estimated Publication–January 2016
First Co-Author
Structural elucidationoftarget pheromoneantheridiogenin the Ceratopteris sexdetermination
regulatory mechanism
VOLUNTEERING
Lafayette Jefferson SpecialNeeds Teaching Aid Fall 2014 -Present
David R. Feltner 72-Hour Bike-a-thon Fall 2014 & 2015
Emergency RoomVolunteer at IU Arnett June 2016-Present
SCHOLARSHIPS AND AWARDS
Triolgy HealthScholarship Fall 2014
Colburn Biochemistry Scholarship Spring 2015
Alva R. Bryant AG AlumniScholarship Spring 2015
Triolgy HealthScholarship Fall 2015
Zygmunt Family Biochemistry Scholarship Spring 2016
Border FamilyScholarship Spring 2016
Purdue ResearchGrant(ARP) Summer 2016
Triolgy HealthScholarship Fall 2016
REFERENCES
Dr. Jody Banks, Professor ofBotany and PlantPathology
banksj@purdue.edu 765-494-5895
Dr. Jeremy Lohman,Assistant ProfessorofBiochemistry
jlohman@purdue.edu 765-494-1087
Andrew Eller, Johns Hopkins ChemicalBiology
aeller1@jh.edu 765-210-9101