The document summarizes a student's research project examining the potential of the CB2 cannabinoid receptor as a target for neuroprotection in Parkinson's disease. The student conducted experiments showing that activating the CB2 receptor with a pharmacological agonist prevented neurodegeneration in an inflammation-driven rat model of Parkinson's. Preliminary analysis of human brain tissue also found microglial activation but was inconclusive about CB2 receptor expression. The student gained experience in laboratory techniques and contributed significantly to the project.
The document describes research investigating how the protein amyloid-beta (Aβ) may amplify itself in Alzheimer's disease. It finds that Aβ inhibits the enzyme ADAM10, reducing cleavage of amyloid precursor protein (APP) and allowing more amyloidogenic processing and Aβ production. It also finds that the protein netrin-1 can disrupt this process, reducing Aβ levels both in vitro and in mouse models. Testing netrin-1 and netrin-mimetic peptides as potential Alzheimer's therapeutics is discussed.
The document discusses a rice resequencing project between The Genome Analysis Centre and researchers in Vietnam. The project aims to sequence rice varieties with interesting phenotypes to help with rice breeding in Vietnam given climate changes. Researchers from Vietnam received training in bioinformatics at TGAC. 18 lanes of sequencing generated 1.3 terabytes of sequence data covering 30x on average for the varieties. SNPs were identified by aligning to reference genomes and filtering, finding on average 0.14% heterozygous SNPs. The results will be made available on TGAC's online browser to help with trait identification and rice improvement in Vietnam.
This document describes the development of a new diagnostic method called ProteAl for the rapid detection of Proteus bacteria. 2-methylbutanal was identified as a volatile organic compound biomarker specifically produced by Proteus. A fluorescent assay was developed using the reagent 5-dimethylaminonaphthalene-1-sulfonylhydrazine to detect 2-methylbutanal. This ProteAl assay could identify Proteus within 7 hours of growth and differentiated it from other common uropathogens. The production of 2-methylbutanal by Proteus was found to be regulated by the isoleucine metabolic pathway. Rational design of growth medium with increased isoleucine enhanced the yield of
Evaluation of infection course in mice induced by L. major in presence of pos...Nanomedicine Journal (NMJ)
This document describes a study that evaluated the infection course in mice induced by L. major parasites in the presence of positively charged liposomes containing CpG oligodeoxynucleotides (CpG ODN). Mice were inoculated with L. major plus various liposome formulations and lesion development and parasite burden were analyzed. The results showed that mice treated with DMPC liposomes containing CpG ODN developed significantly smaller lesions and had lower parasite levels in the spleen and lymph nodes compared to other treatment groups. Additionally, this treatment induced a Th1 immune response profile. Therefore, immune modulation using DMPC liposomes containing CpG ODN may be an effective approach to improve the safety of leishman
Presentation made by Terrence Town, PhD, at the December 12, 2012 webinar hosted by the Alzheimer Research Forum. http://www.alzforum.org/res/for/journal/detail.asp?liveID=207
This document summarizes research on developing an antibody-derived probe for in vivo imaging of tau pathology. Key points:
- Researchers generated libraries of single-chain antibody fragments (scFvs) from a tau-selective monoclonal antibody and identified several phospho-tau-selective scFvs.
- Peripheral injection of one scFv (scFv235) in transgenic tauopathy mice resulted in strong brain signal specifically in areas of tau pathology, correlating with tau aggregates. The signal was weaker for the parent antibody.
- The imaging signal for scFv235 colocalized with markers of endosomes, autophagosomes and lysosomes, suggesting interaction in degradation pathways.
- Such antibody-
This study analyzed corticostriatal projections (CStr) and corticospinal collaterals (CStc) anatomically and functionally. Viral tracing revealed CStc fibers branch within the striatum and synapse onto striatal nuclei. CStr axons also branch extensively and synapse within the striatum and other brain regions. Behaviorally, selectively ablating or silencing CStr showed no impact on motor skill acquisition or performance. Future work will identify shared targets of CStr and CStc, generate 3D models, and test other behavioral assays. This research elucidates the corticostriatal and corticospinal microcircuitry integral to motor function.
A New Frontier of Precision Medicine: Using PET for Image-Guided Neurointerve...InsideScientific
Mice are by far the most frequently used animal for modeling disease and developing therapeutic strategies including neurointerventions. However, due to its anatomical and physiological barriers, the brain is a difficult target for delivery of therapeutic agents. Systemic administration is plagued with marginal brain accumulation and high risk of off-target side effects.
In this webinar sponsored by Scintica Instrumentation, Dr. Piotr Walczak, Dr. Mirosław Janowski and Dr. Wojciech Lesniak address this challenge and discuss why advanced imaging is essential to perform image-guided neurointerventions.
First, Dr. Janowski provides rationale as to how imaging can be used to better understand how therapeutic agents are delivered to the brain and subsequently cleared. Next, Dr. Walczak reviews methodological and technological advances for improving precision and reproducibility of brain targeting in mice based on MRI and two-photon microscopy. Finally, Dr. Lesniak presents recently-published results using ARGUS PET/CT to quantify intra-artrial delivery of antibodies, nanobodies and poly(amidoamine) dendrimers.
Key Topics Include:
- Overview of Pre-Clinical PET/CT imaging
- Examples from applications including oncology, neurology, cardiology and dynamic imaging
- An introduction to SuperArgus PET/CT and what makes it unique
To learn more, go to:
https://insidescientific.com/webinar/precision-medicine-using-pet-image-guided-neurointervention/
The document describes research investigating how the protein amyloid-beta (Aβ) may amplify itself in Alzheimer's disease. It finds that Aβ inhibits the enzyme ADAM10, reducing cleavage of amyloid precursor protein (APP) and allowing more amyloidogenic processing and Aβ production. It also finds that the protein netrin-1 can disrupt this process, reducing Aβ levels both in vitro and in mouse models. Testing netrin-1 and netrin-mimetic peptides as potential Alzheimer's therapeutics is discussed.
The document discusses a rice resequencing project between The Genome Analysis Centre and researchers in Vietnam. The project aims to sequence rice varieties with interesting phenotypes to help with rice breeding in Vietnam given climate changes. Researchers from Vietnam received training in bioinformatics at TGAC. 18 lanes of sequencing generated 1.3 terabytes of sequence data covering 30x on average for the varieties. SNPs were identified by aligning to reference genomes and filtering, finding on average 0.14% heterozygous SNPs. The results will be made available on TGAC's online browser to help with trait identification and rice improvement in Vietnam.
This document describes the development of a new diagnostic method called ProteAl for the rapid detection of Proteus bacteria. 2-methylbutanal was identified as a volatile organic compound biomarker specifically produced by Proteus. A fluorescent assay was developed using the reagent 5-dimethylaminonaphthalene-1-sulfonylhydrazine to detect 2-methylbutanal. This ProteAl assay could identify Proteus within 7 hours of growth and differentiated it from other common uropathogens. The production of 2-methylbutanal by Proteus was found to be regulated by the isoleucine metabolic pathway. Rational design of growth medium with increased isoleucine enhanced the yield of
Evaluation of infection course in mice induced by L. major in presence of pos...Nanomedicine Journal (NMJ)
This document describes a study that evaluated the infection course in mice induced by L. major parasites in the presence of positively charged liposomes containing CpG oligodeoxynucleotides (CpG ODN). Mice were inoculated with L. major plus various liposome formulations and lesion development and parasite burden were analyzed. The results showed that mice treated with DMPC liposomes containing CpG ODN developed significantly smaller lesions and had lower parasite levels in the spleen and lymph nodes compared to other treatment groups. Additionally, this treatment induced a Th1 immune response profile. Therefore, immune modulation using DMPC liposomes containing CpG ODN may be an effective approach to improve the safety of leishman
Presentation made by Terrence Town, PhD, at the December 12, 2012 webinar hosted by the Alzheimer Research Forum. http://www.alzforum.org/res/for/journal/detail.asp?liveID=207
This document summarizes research on developing an antibody-derived probe for in vivo imaging of tau pathology. Key points:
- Researchers generated libraries of single-chain antibody fragments (scFvs) from a tau-selective monoclonal antibody and identified several phospho-tau-selective scFvs.
- Peripheral injection of one scFv (scFv235) in transgenic tauopathy mice resulted in strong brain signal specifically in areas of tau pathology, correlating with tau aggregates. The signal was weaker for the parent antibody.
- The imaging signal for scFv235 colocalized with markers of endosomes, autophagosomes and lysosomes, suggesting interaction in degradation pathways.
- Such antibody-
This study analyzed corticostriatal projections (CStr) and corticospinal collaterals (CStc) anatomically and functionally. Viral tracing revealed CStc fibers branch within the striatum and synapse onto striatal nuclei. CStr axons also branch extensively and synapse within the striatum and other brain regions. Behaviorally, selectively ablating or silencing CStr showed no impact on motor skill acquisition or performance. Future work will identify shared targets of CStr and CStc, generate 3D models, and test other behavioral assays. This research elucidates the corticostriatal and corticospinal microcircuitry integral to motor function.
A New Frontier of Precision Medicine: Using PET for Image-Guided Neurointerve...InsideScientific
Mice are by far the most frequently used animal for modeling disease and developing therapeutic strategies including neurointerventions. However, due to its anatomical and physiological barriers, the brain is a difficult target for delivery of therapeutic agents. Systemic administration is plagued with marginal brain accumulation and high risk of off-target side effects.
In this webinar sponsored by Scintica Instrumentation, Dr. Piotr Walczak, Dr. Mirosław Janowski and Dr. Wojciech Lesniak address this challenge and discuss why advanced imaging is essential to perform image-guided neurointerventions.
First, Dr. Janowski provides rationale as to how imaging can be used to better understand how therapeutic agents are delivered to the brain and subsequently cleared. Next, Dr. Walczak reviews methodological and technological advances for improving precision and reproducibility of brain targeting in mice based on MRI and two-photon microscopy. Finally, Dr. Lesniak presents recently-published results using ARGUS PET/CT to quantify intra-artrial delivery of antibodies, nanobodies and poly(amidoamine) dendrimers.
Key Topics Include:
- Overview of Pre-Clinical PET/CT imaging
- Examples from applications including oncology, neurology, cardiology and dynamic imaging
- An introduction to SuperArgus PET/CT and what makes it unique
To learn more, go to:
https://insidescientific.com/webinar/precision-medicine-using-pet-image-guided-neurointervention/
In vivo modulation of dopaminergic nigrostriatal pathways by cytisine derivat...Georgi Daskalov
This study examined the ability of the nicotinic receptor agonist cytisine and two halogenated derivatives (3-bromocytisine and 5-bromocytisine) to stimulate dopamine release in vivo and protect against dopamine depletion in a rat model of Parkinson's disease. Microdialysis experiments showed that cytisine, 5-bromocytisine, and nicotine were more effective than 3-bromocytisine at inducing striatal dopamine release. Administration of cytisine and 5-bromocytisine before and after an intranigral injection of 6-hydroxydopamine significantly prevented the decrease in striatal dopamine levels, but 3-bromocytisine did not provide protection. These results suggest that the efficacy
1. The study characterized Echinococcus granulosus genotypes from cyst samples collected from buffalo, sheep, and humans in Bangladesh using PCR of mitochondrial genes.
2. Two genotypes were identified: the common sheep strain G1 and the buffalo strain G3. Nine of 15 buffalo samples and 6 of 9 sheep samples tested positive for G1 using 12S rRNA, while 3 buffalo tested positive for G3 using COX1.
3. Sequence analysis revealed close identity between Bangladeshi isolates and reference sequences for G1 and G3 from other countries. This is the first molecular characterization of Echinococcus spp. in Bangladesh.
1. The study tested the toxicity of used coffee grounds on larvae of the mosquito Ochlerotatus notoscriptus at different concentrations. High concentrations induced high larval mortality within days, while low concentrations boosted survivorship.
2. The results suggest used coffee grounds could be a cost-free and environmentally friendly larval control method, but extensive field trials are needed before use is advocated.
3. Nutrient depletion in the laboratory conditions likely caused high mortality in the controls, compromising the results, whereas providing food sustained higher survival rates in previous studies.
1) The document discusses various potential therapies for human rabies that have been studied experimentally, including ketamine, ribavirin, interferon, and therapeutic coma.
2) Experiments in a mouse model found that ketamine did not provide any benefits such as reduced mortality or viral load, and it has also failed to help in at least 8 reported human rabies cases.
3) Similarly, the "Willoughby protocol" of inducing therapeutic coma has not proven effective and should not be continued without further study, as the risks may outweigh any unproven benefits. More research is needed to identify truly promising treatments.
This study investigated how cocaine withdrawal affects anxiety and activity in the dorsal raphe (DR), a brain region involved in serotonin signaling and anxiety regulation. Mice were administered cocaine or saline in a binge-like pattern over 10 days. Anxiety-like behavior was assessed at different withdrawal timepoints using elevated plus maze and open field tests. Electrophysiological recordings from DR serotonin neurons found increased inhibitory currents during withdrawal, especially at 25 hours, indicating heightened GABA activity. Blocking 5-HT2C receptors in the DR prevented both the increased GABA activity and anxiety-like behavior during withdrawal. This suggests 5-HT2C receptors mediate anxiety produced by cocaine withdrawal, potentially by regulating GABA signaling in the DR serotonin system.
Tracing the Origins of Data and Ideas - Provenance Visualization for Biomedic...Nils Gehlenborg
A researcher presented a document on using data visualization and provenance to aid biomedical discovery. Some key points:
- There is significant reproducibility crisis in science according to a Nature survey of researchers.
- Provenance captures the origins and history of data and insights to improve reproducibility and understanding.
- Tools like StratomeX and CLUE were developed to visualize relationships in biomedical data from sources like The Cancer Genome Atlas to guide exploration and discovery.
- The presenter advocates for data-driven discovery, communication, and storytelling using visualization histories ("vistories") to better capture the full process and context of insights.
Michael Chevas, a photographer from Thailand, transformed celebrities from Southeast Asia into iconic Disney characters for 2015. The document features photos of celebrities from countries like Indonesia, Philippines, Thailand, and Malaysia posed as Disney princesses and villains like Cinderella, Mulan, Ariel, Belle, Merida, Jasmine, Maleficent, Tiana, Rapunzel, and Elsa. Each photo is accompanied by a short biography of the celebrity and a Disney quote.
El documento discute la noción de infancia y cómo ha cambiado a través del tiempo. Argumenta que la infancia no es un concepto natural sino histórico, y que en el pasado los niños no experimentaban los mismos sentimientos asociados a la infancia como dependencia y necesidad de amor. Explora cómo los adultos han institucionalizado la infancia y clasificado a los niños, aunque reconoce que factores como el comportamiento y desarrollo del niño también influyen en cómo termina su infancia.
El Estado tiene la responsabilidad de garantizar la educación gratuita y de calidad para toda la población. Los docentes deben brindar una enseñanza de calidad y valerse de los recursos disponibles para asegurar el aprendizaje de los estudiantes, como lo hizo la profesora en la película al esforzarse por encontrar a su estudiante desaparecido. Tanto el Estado como los maestros deben trabajar para que los niños no dejen de estudiar y reciban una educación óptima que se ajuste a los cambios en el sistema escol
P. Sreedharan is seeking a position as a QA/QC Coordinator or Quality Supervisor. He has over 4 years of experience in quality control roles. He holds qualifications in marine engineering, NDT testing, and computer science. Sreedharan is looking to join a progressive company where he can utilize his skills and experience while taking on higher responsibilities. He has experience in inspection, auditing, documentation, and ensuring compliance with quality standards.
El documento define varios términos pedagógicos y educativos, incluyendo utopía, pormenorización, parricidio, abdicación, monopolio, epifenómeno, hegemonía, matriz, escuela, pedagogía, didáctica, alianza, maestro, adaptación y contrato. Cada definición incluye el término, su significado y el autor de la definición.
Dokumen tersebut membahas tentang kewirausahaan kerajinan limbah tekstil, langkah-langkah merancang wirausaha kerajinan limbah, ciri-ciri seorang wirausaha seperti percaya diri, berani mengambil resiko, berorientasi pada tugas dan hasil, kepemimpinan, berorientasi ke masa depan, kreatifitas dan inovasi, serta contoh wirausaha kerajinan limbah tekstil seperti usaha Tomoi.
Dokumen ini membahas tentang kewirausahaan di bidang kerajinan tekstil. Mencakup definisi wirausaha, karakteristik entrepreneur, stimulus berwirausaha seperti potensi akulturasi budaya tekstil Indonesia, serta faktor-faktor penentu keberhasilan dan kegagalan berwirausaha.
This document reports on a study that tested the effects of isoxazole 9 (Isx-9), a small synthetic molecule, on adult hippocampal neurogenesis in rats. The study found that administering Isx-9 for 14 days potentiated cell proliferation and increased the number of immature neurons in the hippocampal dentate gyrus. Isx-9 treatment also completely reversed the reduction in cell proliferation and neuronal commitment observed in vehicle-treated animals that were subjected to repeated handling and injections. These findings demonstrate that Isx-9 has promising pro-neurogenic properties and could help mitigate stress-induced deficits in adult hippocampal neurogenesis.
Publish Your Papers In The Top Scientific Journalsguestd02fab
The document discusses how to publish scientific papers in top journals. It provides advice on what constitutes high-quality research and first-class papers, such as making major advances in important fields, developing new techniques, or challenging existing views. It also discusses the paper evaluation and review process, emphasizing the importance of convincing data, controls, and consideration of alternatives. Cover letters should highlight a paper's significance and suggest reviewers while avoiding conflicts of interest.
UTF-8''Final Assessing post-synaptic partners of Dentate Granule Cells in a M...Grant Pizzo
1) The document describes an experiment assessing how dentate granule cell (DGC) axon terminals and their post-synaptic partners change in a rat model of temporal lobe epilepsy (TLE).
2) The researcher induced seizures in rats using pilocarpine and later injected retroviruses to label dividing DGCs. Tissue was then analyzed to identify post-synaptic partners of DGCs using antibodies targeting interneurons and mossy cells.
3) Preliminary results showed that antibodies for GAD67 and GluR2 successfully labeled the correct cell types, but need further refinement, while an mGluR1/5 antibody did not label interneurons in the dentate gyrus
This document summarizes research investigating the cellular and molecular mechanisms underlying cognitive enhancement induced by a peptide called FGL. The key findings are:
1. FGL activates fibroblast growth factor receptor (FGFR) signaling pathways in vivo and in vitro.
2. FGL enhances long-term potentiation (LTP) in hippocampal slices by facilitating delivery of AMPA receptors to synapses through activation of NMDA receptors.
3. Both the LTP enhancement and cognitive improvement produced by FGL are mediated by initial protein kinase C (PKC) activation followed by sustained calcium/calmodulin-dependent protein kinase II (CaMKII) activation.
4. These results link facilitation
This document summarizes a study that investigated whether melatonin treatment could modify depression-like and anxiety-like behaviors in a mouse model of seasonal affective disorder (SAD). Mice were exposed to short photoperiods to induce SAD-like behaviors and symptoms. Daily melatonin injections before lights off altered the expression patterns of circadian clock genes in the suprachiasmatic nucleus and genes related to serotonin neurotransmission in the dorsal raphe. Melatonin treatment also reduced depression-like behavior in forced swim tests. While melatonin did not strongly phase shift circadian rhythms, it increased the amplitude of oscillations in clock gene expression. The findings suggest modest adjustments to circadian rhythms from melat
Shamili Sammohi has extensive experience in in-vivo, in-vitro, and analytical techniques including mice surgeries, cell culture, Western blotting, and HPLC. She has worked as a research assistant at the University of Cincinnati studying nociception in mice and performed techniques like brain surgeries, DRG culture, immunohistochemistry, and electrophysiology. She holds an MS in Pharmacology and Toxicology from Wright State University and a BS in Pharmacy from Sahasra Institute of Pharmaceutical Sciences in India.
Methods For Improving The Cellular Uptake Of Dna Origami...Christina Santos
The presentation will focus on how the novel "Fifteen Dogs" has been taken up and promoted via Twitter. It will use Anne Freadman's concept of "uptake" to analyze how different Twitter users discuss and spread information about the novel on the platform, effectively promoting the work. The presentation will examine how Twitter serves as a means for novels to be "taken up" and transformed into promotional devices.
In vivo modulation of dopaminergic nigrostriatal pathways by cytisine derivat...Georgi Daskalov
This study examined the ability of the nicotinic receptor agonist cytisine and two halogenated derivatives (3-bromocytisine and 5-bromocytisine) to stimulate dopamine release in vivo and protect against dopamine depletion in a rat model of Parkinson's disease. Microdialysis experiments showed that cytisine, 5-bromocytisine, and nicotine were more effective than 3-bromocytisine at inducing striatal dopamine release. Administration of cytisine and 5-bromocytisine before and after an intranigral injection of 6-hydroxydopamine significantly prevented the decrease in striatal dopamine levels, but 3-bromocytisine did not provide protection. These results suggest that the efficacy
1. The study characterized Echinococcus granulosus genotypes from cyst samples collected from buffalo, sheep, and humans in Bangladesh using PCR of mitochondrial genes.
2. Two genotypes were identified: the common sheep strain G1 and the buffalo strain G3. Nine of 15 buffalo samples and 6 of 9 sheep samples tested positive for G1 using 12S rRNA, while 3 buffalo tested positive for G3 using COX1.
3. Sequence analysis revealed close identity between Bangladeshi isolates and reference sequences for G1 and G3 from other countries. This is the first molecular characterization of Echinococcus spp. in Bangladesh.
1. The study tested the toxicity of used coffee grounds on larvae of the mosquito Ochlerotatus notoscriptus at different concentrations. High concentrations induced high larval mortality within days, while low concentrations boosted survivorship.
2. The results suggest used coffee grounds could be a cost-free and environmentally friendly larval control method, but extensive field trials are needed before use is advocated.
3. Nutrient depletion in the laboratory conditions likely caused high mortality in the controls, compromising the results, whereas providing food sustained higher survival rates in previous studies.
1) The document discusses various potential therapies for human rabies that have been studied experimentally, including ketamine, ribavirin, interferon, and therapeutic coma.
2) Experiments in a mouse model found that ketamine did not provide any benefits such as reduced mortality or viral load, and it has also failed to help in at least 8 reported human rabies cases.
3) Similarly, the "Willoughby protocol" of inducing therapeutic coma has not proven effective and should not be continued without further study, as the risks may outweigh any unproven benefits. More research is needed to identify truly promising treatments.
This study investigated how cocaine withdrawal affects anxiety and activity in the dorsal raphe (DR), a brain region involved in serotonin signaling and anxiety regulation. Mice were administered cocaine or saline in a binge-like pattern over 10 days. Anxiety-like behavior was assessed at different withdrawal timepoints using elevated plus maze and open field tests. Electrophysiological recordings from DR serotonin neurons found increased inhibitory currents during withdrawal, especially at 25 hours, indicating heightened GABA activity. Blocking 5-HT2C receptors in the DR prevented both the increased GABA activity and anxiety-like behavior during withdrawal. This suggests 5-HT2C receptors mediate anxiety produced by cocaine withdrawal, potentially by regulating GABA signaling in the DR serotonin system.
Tracing the Origins of Data and Ideas - Provenance Visualization for Biomedic...Nils Gehlenborg
A researcher presented a document on using data visualization and provenance to aid biomedical discovery. Some key points:
- There is significant reproducibility crisis in science according to a Nature survey of researchers.
- Provenance captures the origins and history of data and insights to improve reproducibility and understanding.
- Tools like StratomeX and CLUE were developed to visualize relationships in biomedical data from sources like The Cancer Genome Atlas to guide exploration and discovery.
- The presenter advocates for data-driven discovery, communication, and storytelling using visualization histories ("vistories") to better capture the full process and context of insights.
Michael Chevas, a photographer from Thailand, transformed celebrities from Southeast Asia into iconic Disney characters for 2015. The document features photos of celebrities from countries like Indonesia, Philippines, Thailand, and Malaysia posed as Disney princesses and villains like Cinderella, Mulan, Ariel, Belle, Merida, Jasmine, Maleficent, Tiana, Rapunzel, and Elsa. Each photo is accompanied by a short biography of the celebrity and a Disney quote.
El documento discute la noción de infancia y cómo ha cambiado a través del tiempo. Argumenta que la infancia no es un concepto natural sino histórico, y que en el pasado los niños no experimentaban los mismos sentimientos asociados a la infancia como dependencia y necesidad de amor. Explora cómo los adultos han institucionalizado la infancia y clasificado a los niños, aunque reconoce que factores como el comportamiento y desarrollo del niño también influyen en cómo termina su infancia.
El Estado tiene la responsabilidad de garantizar la educación gratuita y de calidad para toda la población. Los docentes deben brindar una enseñanza de calidad y valerse de los recursos disponibles para asegurar el aprendizaje de los estudiantes, como lo hizo la profesora en la película al esforzarse por encontrar a su estudiante desaparecido. Tanto el Estado como los maestros deben trabajar para que los niños no dejen de estudiar y reciban una educación óptima que se ajuste a los cambios en el sistema escol
P. Sreedharan is seeking a position as a QA/QC Coordinator or Quality Supervisor. He has over 4 years of experience in quality control roles. He holds qualifications in marine engineering, NDT testing, and computer science. Sreedharan is looking to join a progressive company where he can utilize his skills and experience while taking on higher responsibilities. He has experience in inspection, auditing, documentation, and ensuring compliance with quality standards.
El documento define varios términos pedagógicos y educativos, incluyendo utopía, pormenorización, parricidio, abdicación, monopolio, epifenómeno, hegemonía, matriz, escuela, pedagogía, didáctica, alianza, maestro, adaptación y contrato. Cada definición incluye el término, su significado y el autor de la definición.
Dokumen tersebut membahas tentang kewirausahaan kerajinan limbah tekstil, langkah-langkah merancang wirausaha kerajinan limbah, ciri-ciri seorang wirausaha seperti percaya diri, berani mengambil resiko, berorientasi pada tugas dan hasil, kepemimpinan, berorientasi ke masa depan, kreatifitas dan inovasi, serta contoh wirausaha kerajinan limbah tekstil seperti usaha Tomoi.
Dokumen ini membahas tentang kewirausahaan di bidang kerajinan tekstil. Mencakup definisi wirausaha, karakteristik entrepreneur, stimulus berwirausaha seperti potensi akulturasi budaya tekstil Indonesia, serta faktor-faktor penentu keberhasilan dan kegagalan berwirausaha.
This document reports on a study that tested the effects of isoxazole 9 (Isx-9), a small synthetic molecule, on adult hippocampal neurogenesis in rats. The study found that administering Isx-9 for 14 days potentiated cell proliferation and increased the number of immature neurons in the hippocampal dentate gyrus. Isx-9 treatment also completely reversed the reduction in cell proliferation and neuronal commitment observed in vehicle-treated animals that were subjected to repeated handling and injections. These findings demonstrate that Isx-9 has promising pro-neurogenic properties and could help mitigate stress-induced deficits in adult hippocampal neurogenesis.
Publish Your Papers In The Top Scientific Journalsguestd02fab
The document discusses how to publish scientific papers in top journals. It provides advice on what constitutes high-quality research and first-class papers, such as making major advances in important fields, developing new techniques, or challenging existing views. It also discusses the paper evaluation and review process, emphasizing the importance of convincing data, controls, and consideration of alternatives. Cover letters should highlight a paper's significance and suggest reviewers while avoiding conflicts of interest.
UTF-8''Final Assessing post-synaptic partners of Dentate Granule Cells in a M...Grant Pizzo
1) The document describes an experiment assessing how dentate granule cell (DGC) axon terminals and their post-synaptic partners change in a rat model of temporal lobe epilepsy (TLE).
2) The researcher induced seizures in rats using pilocarpine and later injected retroviruses to label dividing DGCs. Tissue was then analyzed to identify post-synaptic partners of DGCs using antibodies targeting interneurons and mossy cells.
3) Preliminary results showed that antibodies for GAD67 and GluR2 successfully labeled the correct cell types, but need further refinement, while an mGluR1/5 antibody did not label interneurons in the dentate gyrus
This document summarizes research investigating the cellular and molecular mechanisms underlying cognitive enhancement induced by a peptide called FGL. The key findings are:
1. FGL activates fibroblast growth factor receptor (FGFR) signaling pathways in vivo and in vitro.
2. FGL enhances long-term potentiation (LTP) in hippocampal slices by facilitating delivery of AMPA receptors to synapses through activation of NMDA receptors.
3. Both the LTP enhancement and cognitive improvement produced by FGL are mediated by initial protein kinase C (PKC) activation followed by sustained calcium/calmodulin-dependent protein kinase II (CaMKII) activation.
4. These results link facilitation
This document summarizes a study that investigated whether melatonin treatment could modify depression-like and anxiety-like behaviors in a mouse model of seasonal affective disorder (SAD). Mice were exposed to short photoperiods to induce SAD-like behaviors and symptoms. Daily melatonin injections before lights off altered the expression patterns of circadian clock genes in the suprachiasmatic nucleus and genes related to serotonin neurotransmission in the dorsal raphe. Melatonin treatment also reduced depression-like behavior in forced swim tests. While melatonin did not strongly phase shift circadian rhythms, it increased the amplitude of oscillations in clock gene expression. The findings suggest modest adjustments to circadian rhythms from melat
Shamili Sammohi has extensive experience in in-vivo, in-vitro, and analytical techniques including mice surgeries, cell culture, Western blotting, and HPLC. She has worked as a research assistant at the University of Cincinnati studying nociception in mice and performed techniques like brain surgeries, DRG culture, immunohistochemistry, and electrophysiology. She holds an MS in Pharmacology and Toxicology from Wright State University and a BS in Pharmacy from Sahasra Institute of Pharmaceutical Sciences in India.
Methods For Improving The Cellular Uptake Of Dna Origami...Christina Santos
The presentation will focus on how the novel "Fifteen Dogs" has been taken up and promoted via Twitter. It will use Anne Freadman's concept of "uptake" to analyze how different Twitter users discuss and spread information about the novel on the platform, effectively promoting the work. The presentation will examine how Twitter serves as a means for novels to be "taken up" and transformed into promotional devices.
Pablo Gracia is interested in molecular biophysics and how biomolecular structures influence biological systems. He enjoys collaborative academic research and using new ideas to solve longstanding biological mysteries. Gracia has experience in single-molecule fluorescence spectroscopy and applying it to study membrane protein folding mechanisms. He is currently working on membrane protein folding and the unfolded state of membrane proteins using this technique.
This study assessed sub-chronic neuropathological and biochemical changes in the mouse visual system 3 weeks after repetitive mild traumatic brain injury (r-mTBI). Histological analysis found increased cellularity and areas of demyelination in the optic nerves of r-mTBI mice compared to repetitive sham controls. The number of retinal ganglion cells was also decreased by about 25% in r-mTBI mice. Proteomic analysis of optic nerves showed changes indicating effects on neuronal processes like microtubule depolymerization and filament disassembly. Lipidomic analysis found changes in phospholipid species, including an increase in lysophosphatidylcholine, a demyelinating agent. The results indicate
This document contains summaries of three laboratory assignments:
1) A column chromatography laboratory that taught protein isolation techniques and showed lysozyme purification.
2) A neurobiology workshop that covered the nervous system, neurons, and included a sheep brain dissection.
3) An in-silico drug design workshop where students identified benzene clusters that could bind to and inhibit the HIV protease protein.
This study tested the hypothesis that combining increased intrinsic growth ability through PTEN deletion with reduced extrinsic growth inhibition through Nogo deletion may further improve axonal regeneration after spinal cord injury compared to manipulating either factor alone. The results showed that PTEN/Nogo codeletion enhanced corticospinal tract regeneration more than PTEN deletion alone but did not further increase sprouting compared to PTEN deletion. While regeneration and sprouting increased in PTEN-deleted and PTEN/Nogo-deleted mice, functional recovery was only temporarily improved or not improved, indicating additional strategies are needed to translate enhanced axonal growth into functional benefits.
Summary
Neurodevelopment is a complex process governed by both intrinsic and extrinsic signals. While historically studied by researching the brain, inputs from the periphery impact many neurological conditions. Indeed, emerging data suggests communication between the gut and the brain in anxiety,
depression, cognition and autism spectrum disorder (ASD). The development of a healthy, functional brain depends on key pre- and post-natal events that integrate environmental cues, such as molecular signals from the gut. These cues largely originate from the microbiome, the consortium of symbiotic bacteria that reside within all animals. Research over the past few years reveals that the gut microbiome plays a role in basic neurogenerative processes such as the formation of the blood-brainbarrier, myelination, neurogenesis, and microglia maturation, and also modulates many aspects of animal behavior. Herein, we discuss the biological intersection of neurodevelopment and the microbiome, and explore the hypothesis that gut bacteria are integral contributors to development and function of the nervous system, and the balance between mental health and disease.
Salvatore Parrino received his Ph.D in Molecular and Cellular Biology from Stony Brook University in 2015. His thesis focused on determining the molecular pathways that control Candida albicans morphological switching. He has extensive teaching experience, including as a graduate TA, instructor for high school biology labs, and assistant lecturer. Parrino has also mentored undergraduate researchers and published papers on novel roles for GlcNAc in cell signaling and cAMP-independent pathways that promote hyphal growth in C. albicans.
This study examined patterns of cortical activity using four different EEG testing protocols: an extended 8-minute baseline with alternating eyes open and closed, viewing a 10-minute dyadic social interaction video, and completing a personality survey. The protocols elicited different levels of activity in the alpha band and theta/beta ratio. Specifically, all protocols differed significantly in alpha activity, and the three eyes-open conditions still differed when just compared to each other. This suggests protocol design impacts EEG results and more representative real-world designs should be explored.
Translational Neuroscience Approach in psychiatry..pptxkrishray616
Translational neuroscience aims to bridge the gap between fundamental scientific research and clinical applications to treat neurological disorders. It focuses on applying discoveries from preclinical research, like animal and cell models, to develop new therapies. Translational neuroscience research occurs across both wet labs, which use experimental techniques, and dry labs, which analyze data through computational methods. The goal is to more quickly translate basic scientific findings into clinical applications that can improve patient outcomes. Identifying biomarkers that indicate disease mechanisms or predict treatment responses is an important part of translational research efforts.
1. Researchers injected mice with a virus that causes neurons activated during exploration of a novel context to express channelrhodopsin-2 (ChR2). They labeled dentate gyrus neurons activated during exploration of context A with ChR2.
2. On a later day, they optically stimulated the neurons labeled in context A while delivering a foot shock in a different context B, to test if this could generate a false fear memory for context A.
3. When later tested, mice that received optical stimulation of dentate gyrus neurons showed increased freezing in context A, where no shock was delivered, demonstrating the creation of a false fear memory through artificial means.
Mattias Gustafsson is a 43-year-old microbiologist with a Ph.D. from Lund University and 13 years of postdoctoral experience. He has extensive experience conducting biomedical research in microbiology and infection biology. Gustafsson has held several research roles and has been productive, publishing numerous scientific articles. He is curious, creative, and able to take on both leading and supportive roles in projects.
INDUCED PLURIPOTENT STEM CELL AND GENOME ENGINEERINGharrisonjoshua
This document summarizes a presentation on induced pluripotent stem cells (iPSCs) and genome engineering. It defines iPSCs as adult cells that have been reprogrammed to an embryonic stem cell-like state through the introduction of certain genes. The document discusses how iPSCs can be generated and differentiated into various cell types, offering potential applications in disease modeling, drug development, and regenerative medicine. It also presents several case studies demonstrating how iPSC technology could be applied to develop treatments for retinal pigmentosa, frontotemporal dementia, and preclinical toxicology screening of new drugs.
Isolated central nervous system tuberculosis is uncommon in immunocompetent patients. It resembles a pyogenic brain abscess
clinically and radiologically and poses a problem in diagnosis and treatment. Here we described a case of recurrent frontal lobe
abscess, which was diagnosed as a tubercular abscess. There was no clinical or radiological evidence of active tuberculosis
elsewhere in the body. The diagnosis of tubercular abscess was confirmed by Mycobacterium tuberculosis by Polymerase Chain
Reaction (TB-PCR) in the abscess material aspirated via a burr hole.
Microdialysis is an integral part of preclinical research to determine extracellular fluid and blood concentrations of metabolites, hormones, drugs, etc, and is often used in quantifying the biochemistry of brain and peripheral tissues. However, it is a molecular-only technique and other imaging modalities are needed to provide the researcher with functional and anatomical information of the animal in vivo.
Cellvizio® LAB Fibered Fluorescence Microscope and Microdialysis Research
Kelly Moore - HRB Report FINAL
1. HRB Summer Student Scholarships2015
Student Report Form
HRB SUMMER STUDENT SCHOLARSHIP 2015
Scholars’ Report
(Please complete this form and email as an attachment to summerstudents@hrb.ie by Friday, 16 October 2015)
1. Name of student: Kelly Moore
2. Name of University/ Higher Education Institute where student is studying:
National University of Ireland, Galway
3. Degree course and stage reached:
4. 3rd year Undeminominated Science
5. Name and address of University/Higher Education Institute where research was undertaken:
National University of Ireland, Galway
6. Country in which research was undertaken:
7. Ireland
8. Name and address of supervisor (in Ireland):
Dr. Eilís Dowd,
Pharmacology & Therapeutics
National University of Ireland, Galway
7. Project Title:
The potential of theCB2 subtype ofcannabinoidreceptoraspharmacologicaltargetfordiseasemodification
and neuroprotection in Parkinson's disease
8. Project aims/Hypothesis: (maximum 100 words)
The aim of my project was to validate the CB2 subtype of cannabinoid receptor as a potential
pharmacological target for disease modification and neuroprotection in Parkinson's disease. The
specific objectives were 1) to determine if pharmacological targeting of the CB2 receptor would
prevent nigrostriatal neurodegeneration in a rat model of Parkinson’s disease,and 2) to determine
if the CB2 receptor is expressed in the substantia nigra of post mortem human patients' brains. The
hypothesis being tested in this study is that activation of the CB2 receptor willprevent Parkinsonian
neurodegeneration thus highlighting its potential as a target for disease modification in this
disease.
2. HRB Summer Student Scholarships2015
Student Report Form
9. Project Description: (maximum 1,000 words) to include abstract (summary); aims and objectives;
materials and methods; results; discussion; conclusion. Use tables, diagrams or photographs
where appropriate (these are not included in the word limit)
Background: The endocannabinoid system has recently emerged as a potential target to curb the
chronic self-sustaining cycle of neuroinflammation and neurodegeneration observed in
Parkinson’s disease (Ashton & Glass, 2007; Benito et al., 2008; Ehrhart et al., 2005; Kannarkat et
al., 2013; Tansey & Goldberg, 2010). Moreover, the Dowd group has recently shown that this
receptor significantly upregulated in an inflammation-driven rat model of this condition
(Concannon et al., 2015).
Aim: Therefore, the aim of this study was to determine if pharmacological activation of the CB2
receptor in this inflammation-driven model could prevent nigrostriatal neurodegeneration. We
also completed some preliminary studies to determine if the CB2 receptor is expressed in the
substantia nigra of post mortem human patients' brains in order to maximise the translational
relevance of our study.
Methods: Male Sprague Dawley rats were assigned to four groups: [1] Naive & Vehicle (n=4), [2]
Naive & CB2 agonist (n=4), [3] LPS lesion & Vehicle (n=10), [4] LPS lesion & CB2 agonist (n=10).
Animals in the lesion groups received a unilateral, intra-nigral LPS lesion (10 µg in 2 µl). Animals
were injected with either CB2 agonist (JWH-133, 1 mg/kg i.p.) or vehicle (sterile saline), 2 hr prior
to surgery, and daily for a further 13 days, and were then sacrificed 14 days post-surgery.
Quantitative immunohistochemical analysis was performed to assess neuroinflammation and
neurodegeneration in the nigrostriatal pathway. For the human brains, preliminary
immunohistochemical staining for microglia and the CB2 receptor was carried out on brain sections
procured from the Dublin Brain Bank.
Results: Injection of LPS into the rat substantia nigra caused significant nigral microgliosis (Fig. 1)
and nigrostriatal neurodegeneration (Fig. 3) but no astrocytic scarring (Fig. 2). Chronic JWH-133
attenuated LPS-induced dopaminergic neurodegeneration at the level of the cell bodies in the
substantia nigra. Interestingly, the CB2 agonist did not reduce microglial infiltration/proliferation
at the lesion site, indicating that the protective effects of JWH-133 treatment may involve other
anti-inflammatory mechanisms such as reduction in release of pro-inflammatory mediators from
these cells. In the human substantia nigra, clear microglial staining was observed but the CB2
staining was not conclusive (Fig. 4).
Conclusion: Overall, this study has shown that pharmacological targeting of the CB2 receptor
protects the nigrostriatal pathway from inflammation-driven dopaminergic neurodegeneration via
a mechanism that does not involve a reduction in microgliosis. This study indicates that targeting
the CB2 receptor may represent a viable target for anti-inflammatory disease modification in
Parkinson’s disease.
3. HRB Summer Student Scholarships2015
Student Report Form
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Fig. 1 Immunohistochemical analysis for microglia. OX-42 immunohistochemistry revealed that LPS
induced nigral microgliosis but this was not reduced by the CB2 agonist JWH-133.
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4. HRB Summer Student Scholarships2015
Student Report Form
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Fig. 2 Immunohistochemical analysis for astrocytes. GFAP immunohistochemistry revealed that LPS did
not induce any nigral astrocytosis.
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5. HRB Summer Student Scholarships2015
Student Report Form
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Fig. 3 Immunohistochemical analysis for nigrostriatal degeneration. Tyrosine hydroxylase
immunohistochemistry revealed that LPS caused significant nigrostriatal neurodegeneration, and
importantly, this was prevented by the CB2 agonist JWH-133.
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6. HRB Summer Student Scholarships2015
Student Report Form
Fig. 4 Immunohistochemical staining of human brain sections. Iba1 staining revealed clear microgliosis
in the human substantia nigra (left photomicrograph). However, as no clear cellular morphology
was observed after CB2 receptor staining (right photomicrograph), we could not definitively
conclude that the CB2 receptor was expressed in the human Parkinsonian brain.
7. HRB Summer Student Scholarships2015
Student Report Form
10. Overview of Research Experience:
My overall experience as a HRB Summer Scholar was extremely positive. I gained invaluable
experience while working in the Dowd laboratory. In my first week, I got an excellent introduction
to the lab - I got to seehow a lab is run efficiently and I met allthe brilliant people who work and/or
are studying there. I got a broad exposure to various aspects of lab work and research, and I now
have a major appreciation for all the research carried out with a laboratory setting, the time put
in, and the cost of each project. I found the lab a very friendly environment, and staff and students
were more than willing to help when I had any difficulty. If I encountered a problem that I couldn’t
resolve myself, the other lab members were always very helpful and informative. However, they
also encouraged me to figure out some problems on my own, this helped with my own self
confidence and competence in the lab.
I also got great experience of working in a team and I got an insight into the importance of
teamwork. I got run my own immunohistochemistry assay to obtain new data and also got the
opportunity to analyse existing immunohistochemical slides already obtained. This gave me a
greater understanding of the bigger picture of the project. My project was part of an existing
project (funded by the HRB (HRA_POR/2012/12)) and will contribute significantly to the future
direction of the Dowd team. As my research showed that activation of the CB2 receptor could
prevent LPS-induced nigrostriatal neurodegeneration, this could have important implications for
the development of new drugs for Parkinson’s disease in the future.
By chatting to the research students and staff I was able to gain a greater understanding of the
world of research and I know have better knowledge of the work it entails.This gives me the ability
to make more educated decision on my own future career. From my eight weeks working in the
lab, I can now say with certainty that I would be interested in a career in research.
11. Describe the role played by you in the project:
I played a big role in project and gained significant new skills that will be invaluable to me in my
future career such as laboratory animal maintenance, brain sectioning, immunohistochemistry,
immunofluorescence, microscopy, Image J analysis, data presentation/statistical analysis, and
report writing. Specifically:
1. I got the opportunity also to observe and help with the maintenance of live animals in the lab.
2. I got to use the microtome to section rat brain samples at 30 mm.
3. I preformed the GFAP immunohistochemistry for astrocytes from start to finishindependently.
Moreover, I also captured the photomicrographs, used Image J to graph and analyse the data,
and I drew my own conclusions based on these findings.
4. I also got substantially more experience in data analysis and presentation as I reanalysed the
previously generated photomicrographs of the OX42 and tyrpsine hydroxylase
immunostaining.
5. I also worked with the human brain sections which I found particularly interesting. Specifically,
I completed the microglial and CB2 staining independently from start to finish.
12. References (maximum 8):
1. Ashton JC, Glass M.
8. HRB Summer Student Scholarships2015
Student Report Form
The cannabinoid CB2 receptor as a target for inflammation-dependent neurodegeneration.
Curr Neuropharmacol. 2007;5(2):73-80.
PMID:18615177
2. Benito C, Tolón RM, Pazos MR, Núñez E, Castillo AI, Romero J.
Cannabinoid CB2 receptors in human brain inflammation.
Br J Pharmacol. 2008 Jan;153(2):277-85. Epub 2007 Oct 15. Review.
PMID:17934510
3. Concannon RM, Okine BN, Finn DP, Dowd E.
Differential upregulation of the cannabinoid CB₂ receptor in neurotoxic and inflammation-driven
rat models of Parkinson's disease.
Exp Neurol. 2015 Jul;269:133-41. doi: 10.1016/j.expneurol.2015.04.007. Epub 2015 Apr 18.
PMID:25895887
4. Ehrhart J, Obregon D, Mori T, Hou H, Sun N, Bai Y, Klein T, Fernandez F, Tan J, Shytle RD.
Stimulation of cannabinoid receptor 2 (CB2) suppresses microglial activation.
J Neuroinflammation. 2005 Dec 12;2:29.
PMID:16343349
5. Kannarkat GT, Boss JM, Tansey MG.
The role of innate and adaptive immunity in Parkinson's disease.
J Parkinsons Dis. 2013;3(4):493-514. doi: 10.3233/JPD-130250. Review.
PMID:24275605
6. Tansey MG, Goldberg MS.
Neuroinflammation in Parkinson's disease: its role in neuronal death and implications for
therapeutic intervention.
Neurobiol Dis. 2010 Mar;37(3):510-8. doi: 10.1016/j.nbd.2009.11.004. Epub 2009 Nov 10. Review.
PMID:19913097
13. Please list any new skills acquired:
Brain sectioning
Immunohistochemistry
Immunofluorence
Photomicroscopy
Image J analysis
Data Analysis
14. Presentations, posters, conference abstracts, or publications arising from the research (if any):
n/a