Recommended
Recommended
More Related Content
What's hot
What's hot (20)
Similar to Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immuno-inhibitory receptor singlec-8
Similar to Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immuno-inhibitory receptor singlec-8 (20)
Recently uploaded
Recently uploaded (20)
Isolation, identification, and characterization of the human airway ligand for the eosinophil and mast cell immuno-inhibitory receptor singlec-8
- 1. Manuela zorrilla castrillón Maylin yofaidy palacios murillo Universidad pontificia bolivariana- 3 semestre
- 2. 2
- 3. DMBT1: deleted in malignant brain tumors 1 SIGLEC-8: induces immune inhibition 3 Link: https://www.jacionline.org/article/S0091-6749(18)31741-X/fulltext
- 4. 4 Objective: Our aim was to identify the major Siglec-8 sialoglycan ligand on the mucus layer of human airways https://www.slideshare.net/RashmiPriyemSaravana/histology-of-trachea
- 5. MÉTODOS-Inmunohistoquímica 5 Se basa en la reacción antígeno anticuerpo para detectar Proteínas “Hacer visible un antígeno” https://www.cancerquest.org/es/para-los-pacientes/det eccion-y-diagnosis/inmunohistoquimica
- 6. MÉTODOS-Electroforesis 6 “Separación de proteínas” Separación de moléculas como las proteínas en base a su peso o carga eléctrica. ● Acrilamida y agarosa Link: electroforesis partes - Bing images
- 7. MÉTODOS-Blot 7 “Medir el tamaño de las proteínas” Se debe realizar una electroforesis y se incuban anticuerpos junto a las proteínas de interés ● Se identifica la proteína a la cual está dirigida el anticuerpo Linkwestern blotting - Búsqueda de Google
- 8. MÉTODOS- Ensayo de inhibición 8 “Evaluación de la actividad enzimática” Link: enzimas - Búsqueda de Google Se evaluó la reducción o eliminación del siglec-8 gracias a la actividad enzimática Sialidase, keratanase I, keratanase II
- 9. Resultados- inmunohistoquímica 9 Cartílago glándulas submucosas Agrecano está presente en el cartílago y los receptores ausentes en las glándulas submucosas = NO apoptosis de eosinófilos ni degranulación mastocítica
- 10. Resultados - Electroforesis 10 ● (A) Mayor expresión siglec-8 en muestra 52. ● (B) Reacción antígeno-anticuerpo Disminuye la expresión con anti-queratán sulfato
- 12. Resultados - ensayo de inhibición 12
- 13. Discussion 13 AUTOR WHAT HE SAID AGREE OR DISAGREE Kerr SC, et al. Siglec-8 is expressed selectively on eosinophils and mast cells on human inflamed airways, where cross-linking induces eosinophil apoptosis and inhibits mast cells Duan S, Paulson JC. The Siglec family of immune cell surface receptors and the extracellular sialoglycans to which they bind provide receptor- ligand interactions that modulate immune responses. Propster JM, et al. Siglec-8 is highly specific in its structural specificity for sialoglycan binding, requiring the precise juxtaposition of a sialic acid and a sulfate carried on the same subterminal galactose.
- 14. Conclusions 14 “Molecular biology is a branch of great importance for medicine, thanks to this it is possible to carry out in-depth studies on molecules, genes and, as in this case, some receptors that may be involved in very frequent diseases such as respiratory diseases, all this driven by technological advances that, although they have been of vital importance, are not yet at their peak. Despite all the knowledge that we have every day, new discoveries are made that constitute great advances for human beings and that drive their development”.
- 16. 16 Maylin yofaidy palacios murillo