This document is a presentation of a short-term industrial training completed at the Royal Research Center in Navsari, Gujarat, India. It describes the training undertaken to fulfill requirements for a Bachelor of Pharmacy degree from Mewar University. The training involved learning about instrumentation like HPLC and gaining hands-on experience operating equipment. Royal Research Center is introduced as a contract research organization with state-of-the-art facilities and equipment for analytical testing and research. The principles and types of chromatography, specifically HPLC, are explained along with the typical instrumentation components like pumps, columns, detectors, and data recorders.

1. A PRESENTATION OF SHORT- TERM INDUSTRIAL TRAINING
AT
Submitted to MEWAR UNIVERSITY in Partial Fulfillment of the requirement for the
award of the degree of BACHELOR OF PHARMACY
DEPARTMENT OF PHARMACY
SUBMITTED BY
AUWAL MOHAMMED KARGO
ROLL NO:-193840010015
ENROLL NO :- MUR1900313

2. blank

3. CANDIDATE DECLARATION
I, MOHAMMED AUWAL KARGO hereby declare that I have undertaken short term
industrial training at Royal Research Center, Navsari (Gujarat) during a period of May
month in partial fulfillment of requirements for the award of degree of B. Pharm at
MEWAR UNIVERSITY, GANGRAR. The work to be presented to the Department of
Pharmacy at MEWAR UNIVERSITY, GANGRAR is an authentic record of training
work.
MOHAMMED AUWAL KARGO MUR1900313
Faculty of Pharmaceutical Science
Mewar University, Chittorgarh.

4. ACKNOWLEDGEMENT
In the course of this training, I have received help from a number of people. First of
all, I would like to thanks them all.
I wish to express my sincere gratitude to Mr. Manish Mishra, Director of Royal
Research Center and Dr. Kamal Kumar Goyal, managing Director of Royal
Research Center providing me an opportunity to do my industrial training. I am
deeply indebted to Dr. Gaurav Kumar Sharma (HoD, Department of Pharmacy) and
Mr. Pankaj Chasta (Counsellor) whose encouragement made during professional
and personal fronts. I cannot forget the efforts made by them in persuasion during
training.
I also thank the Dr. Ashok Kr. Gadiya, chairman of Mewar University for
providing me the opportunity to embark this training and I am grateful to our training
and placement department for giving me an opportunity to do this training.

5. ABOUT THE COMPANY
Royal Research Center
RRC is an Indian Modern and Progressive Contract Research Organization (CRO), meeting the needs of clients with the
highest flexibilities, located in Navsari (GUJARAT). Our highly qualified dedicated staff works with state- of-the-art
facilities provides the most reliable and timely results. Our exceptionally fast turnaround times, rush testing, client
confidentiality, and customer support all contribute to our strengths as an internationally recognized and respected research
center.
RRC can provide you with online test results with the expertise and Education that you require throughout the different
steps of the manufacturing process, from raw materials to the finished products.
They have a well equipped State-of-the art research lab with highly sophisticated instrumentations to design, formulate and
evaluate drug delivery systems using in vitro experimental setups. RRC provides training to research professionals
working in Pharma and Biotech industry as well as to the students of Pharmacy (B. Pharmacy/M. Pharmacy). We provide
them a working platform/lab resource to complete their research projects related to their graduate and postgraduate courses
to sharpen their practical & instrumental skills as well as to add up the basic concepts, instrumental analysis, formulation
and evaluation, production, regulatory affair, skill development, experiments will be performed in small groups as well as
individually. At the end of training an interactive session or group discussion to be held to assess the training programme.

6. HPLC
High Performance Liquid
Chromatography

7. INTRODUCTION
Chromatography:
May be defined as a scientific technique for the
separation of a mixture by passing it in solution or
suspension through a medium in which the components
move at different rates.
It has a stationary and a mobile phases

8. TYPES OF CHROMATOGRAPHY
A. Based on modes chromatography
1. Normal phase mode
2. Reverse phase mode
B. Based on principle of separation
1. Adsorption chromatography
2. Ion exchange chromatography
3. Partition chromatography
4. Size exclusion

9. C. Based on elution technique
1. Isocratic separation
2. Gradient separation
D. Based on the scale of operation
1. Analytical HPLC
2. Preparative HPLC
E. Based on the type of analysis
1. Qualitative analysis
2. Quantitative analysis

10. HPLC CHROMATOGRAPHY
Is a type of chromatography that stands for
 High Performance Liquid Chromatography
 It has two phases, viz; Mobile and Stationary Phases
 A form of Liquid Chromatography used to separate compounds that are dissolved in solution
 HPLC instruments consist of a reservoir of mobile phase, a pump, an injector, a column,
detector and a data recording and processing device.
 Compounds are separated by injecting a sample mixture onto the column
 Separation is based on Partition and or adsorption of compounds towards stationary phase
and mobile phase depends upon the compounds affinity toward either phase.

11. Types of HPLC
 Normal Phase HPLC
In this type, the Stationary Phase is POLAR while the Mobile
Phase is NON-POLAR.
 Reverse Phase HPLC
Here, the Stationary is NON-POLAR while the Mobile Phase is
POLAR.

12. INSTRUMENTATION OF HPLC
Fig. 1: High Performance Liquid Chromatography

13. Fig. 2

14. HPLC Pump

15. Column

16. HPLC Injector

17. UV detector

18. PhotoDiode Array Detector PDA

19. Electrochemical Detectors
Thermal Conductivity Detector
Differential refractometer
Fluorescence detector
Other types of Detectors

20. Recorder
Chromatogram

21. Advantages of HPLC Chromatogram
 High sensitivity
 High performance
 Rapid process, hence time saving
 Can be used for qualitative as well as quantitative
analysis
 Can be used for both analytical and preparative
purpose

22. Automated HPLC

23. Manual HPLC

24. THANK YOU