In vitro fertilization (IVF) is a process where egg cells are fertilized by sperm cells outside of the body. The first successful IVF procedure resulted in the birth of Louise Brown in England in 1978. IVF involves collecting egg and sperm cells, fertilizing the egg with sperm in a laboratory culture medium, and transferring the embryo into the uterus. IVF is used to help infertile couples have children and can also be used for genetic screening of embryos. While effective, IVF requires a high degree of medical expertise and is an expensive procedure.

1. Topic –IN VITRO FERTILIZATION
Presented by:-
Naveen Chaudhary
LPU ,JALANDHAR
M.Sc microbiology

2.  In vitro fertilization (IVF) is a process
by which egg cells are fertilized
by sperm cells outside the body.
Robert G Edwards. 25 July, 1978

3. Louise Brown,England

4. LOUISE BROWN,ENGLAND

5. First test tube baby in
india
Durga,kolkata

6. First IVF animal birth was
of rabbit in 1959 ,china

7. 1.Collection of oocytes
HCG:-Human chorionic gonadotrophin

8. 2 collection of sperms
centrifugation

9. 3. Fertilization
75000:1
18 hrs

10. Media used in IVF
Earl s Solution
Ham's F10
Whitten's medium
Whittingham s medium

11. 4.EMBROY TRANSFER
Teflon catheter,6-8 cells
3-5 days

12. APPLICATIONS
 Production of embryos from valuable
animals that are infertile.
 Genetic screening of embryos using the
techniques of sperm selection and PGD
before embryo transfer.
 To cure genetic diseases in embryos by
replacing faulty sections of DNA with
healthy DNA by GLT

13. LIMITATIONS
Cost
high degree expertise

14.  REFERENCES
 Awoni Awonuga Govindbhai Heidi Birchand Bert Stewart (2001)
,Contamination of EmbryoTransfer Catheter andTreatment Outcome in
InVitro Fertilization, Journal of assisted reproduction and genetics,vol
4,pp.234-345.
 2.Quinn P Kerin JFWarnes GM(2011),Improved pregnancy rate in
human in vitro fertilization with the use of a medium based on the
composition of human tubal fluid,The New England journal of
medicine,vol 44,pp. 483-485.
 3.S P sures, K m, Nai (2009), Side effect of in vitro fertilizabilty of mature
oocyte,Indian journal of biotechnology,vol 5, pp.34-39.
 4 .Michael Hansen, M.P.H., Jennifer J. Kuringczuk (2008) ,The Risk of
Major Birth Defects after Sperm Injection and inVitro Fertilization,The
New England journal of medicine, vol 6,pp.725-738.
 5.Josiane van der,Mark dhont,Zhan dao (2007) ,The efficacy of the
embryo transfer catheter in IVF and ICSI is operator-dependent: a
randomized clinical trial,vol 24,pp -880 887