This document provides a quick guide for immunohistochemistry (IHC) staining using BioGenex Super Sensitive Polymer-HRP kits. It outlines the key steps in IHC staining including tissue baking, dewaxing, antigen retrieval, blocking steps, primary antibody incubation, polymer-HRP detection, DAB staining, hematoxylin counterstaining, and mounting. It also lists available detection systems and kit sizes for manual and automated staining.
Biochar is a very promising material for the "long term" storage of Carbon Dioxide, a greenhouse gas. It has been proved a very good soil amendment material, promoting the soil health, the microorganisms habitats, and water retention. This may be the perfect technology for the final disposition of solid waste, both from municipal and agricultural sources. Biochar is also a by-product in the 'green' renewable energy production. In my institution we are now studying the effects the molecular ecology of soil phosphorous mobilizing bacteria, where biochar might play an important role. Applications of biochar in engineered systems for bioremediation are also under development. Thanks to DGEST, ITS, and COECYT-Coahuila for the support.
Thermostability enhancement of Luciola mingrelica firefly luciferase by in vi...Mikhail Koksharov
Thermostability enhancement of Luciola mingrelica firefly luciferase by in vivo directed evolution
16th International Symposium on Bioluminescence and Chemiluminescence; Lyon, France; April 21, 2010
Biochar is a very promising material for the "long term" storage of Carbon Dioxide, a greenhouse gas. It has been proved a very good soil amendment material, promoting the soil health, the microorganisms habitats, and water retention. This may be the perfect technology for the final disposition of solid waste, both from municipal and agricultural sources. Biochar is also a by-product in the 'green' renewable energy production. In my institution we are now studying the effects the molecular ecology of soil phosphorous mobilizing bacteria, where biochar might play an important role. Applications of biochar in engineered systems for bioremediation are also under development. Thanks to DGEST, ITS, and COECYT-Coahuila for the support.
Thermostability enhancement of Luciola mingrelica firefly luciferase by in vi...Mikhail Koksharov
Thermostability enhancement of Luciola mingrelica firefly luciferase by in vivo directed evolution
16th International Symposium on Bioluminescence and Chemiluminescence; Lyon, France; April 21, 2010
Wild Type and Mutated BRCA - Differentiation of Breast Cancer - BioGenexVictoria Miller
https://info.biogenex.com/wild-type-and-mutated-brca-differentiation-of-breast-cancer-using-new-mirna-biomarker-panel
breast cancer, wild type breast cancer, BRCA mutated breast cancer , BRCA gene mutation.Wild Type and Mutated BRCA , Differentiation of Breast Cancer, brca mutated breast cancer, brca mutation breast cancer
Differentiation of triple-negative breast cancer - BioGenexVictoria Miller
Differentiation of triple-negative breast cancer, Types of triple negative breast cancer, Triple negative breast cancer, Triple negative: breast cancer, triple negative breast cancer.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Wild Type and Mutated BRCA - Differentiation of Breast Cancer - BioGenexVictoria Miller
https://info.biogenex.com/wild-type-and-mutated-brca-differentiation-of-breast-cancer-using-new-mirna-biomarker-panel
breast cancer, wild type breast cancer, BRCA mutated breast cancer , BRCA gene mutation.Wild Type and Mutated BRCA , Differentiation of Breast Cancer, brca mutated breast cancer, brca mutation breast cancer
Differentiation of triple-negative breast cancer - BioGenexVictoria Miller
Differentiation of triple-negative breast cancer, Types of triple negative breast cancer, Triple negative breast cancer, Triple negative: breast cancer, triple negative breast cancer.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
DERIVATION OF MODIFIED BERNOULLI EQUATION WITH VISCOUS EFFECTS AND TERMINAL V...Wasswaderrick3
In this book, we use conservation of energy techniques on a fluid element to derive the Modified Bernoulli equation of flow with viscous or friction effects. We derive the general equation of flow/ velocity and then from this we derive the Pouiselle flow equation, the transition flow equation and the turbulent flow equation. In the situations where there are no viscous effects , the equation reduces to the Bernoulli equation. From experimental results, we are able to include other terms in the Bernoulli equation. We also look at cases where pressure gradients exist. We use the Modified Bernoulli equation to derive equations of flow rate for pipes of different cross sectional areas connected together. We also extend our techniques of energy conservation to a sphere falling in a viscous medium under the effect of gravity. We demonstrate Stokes equation of terminal velocity and turbulent flow equation. We look at a way of calculating the time taken for a body to fall in a viscous medium. We also look at the general equation of terminal velocity.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
2. Baking DeWax
An-gen
Retrieval
Peroxide
Block
Power Block
Immunohistochemistry (IHC) Quick Guide#
Super SensitiveTM Polymer-HRP kits.
2
Primary
An-body
Polymer
HRP
DAB Hematoxylin Moun-ng
Overnight, 50-60oC
then
15-30 min, 60-80oC
Before staining
EZ-DeWax 3 x 3 min, RT For 95% of BioGenex an-bodies
Use EZ-AR -2 Elegance- 20 min, 95oC
See Catalog for an-body-specific
recommenda-on
5-10 min, RT 0-10 min, RT
For >90% of BioGenex
an-bodies: 30 min @ RT
See Datasheet for
an-body-specific
Incuba-on condi-ons
30 min, RT
or
20 min, 37oC
5-10 min, RT 1-3 min, RT Super Mount® Medium
Or
Xmount (for Automa-on)
Super
Enhancer##
20 min, RT
Ki-67 staining in Tonsil /ssue
# For manual use with FFPE -ssue slides. Washing steps omibed for simplicity- for full protocol see Poly-HRP kit datasheet. Op-miza-on
may be required due to varia-on in -ssues/processing.
## Not Required when using OneStep Poly-HRP Detec-on System
4. 4
BioGenex Immunohistochemistry (IHC) Quick Guide
Super SensitiveTM Link-Label kits
7. (cont.) conjugated to a polymerized horse radish peroxidase (Poly-HRP).
The 2-step kit requires pre-incuba-on with Super Enhancer to enhance
binding. As the system is not based on the bio-n-avidin system, problems
associated with endogenous bio-n are completely eliminated. The
technology allows excellent cell penetra-on ability compared with other
polymer-HRPs.
8. DAB (HK542-XAKE) – DAB (3,3'-diaminobenzidine tetrahydrochloride) is
a chromogenic peroxidase substrate. HRP catalyzes the oxida-on of DAB
to form a brown precipitate which stains the -ssue in close proximity to
the HRP conjugated an-body. DAB is insoluble in alcohol, xylene or water
and therefore is compa-ble with most clearing agents and moun-ng
media.
9. Hematoxylin (HK100-5K, HK100-9K) – (BioGenex Mayer’s hematoxylin is a blue nuclear counterstain that makes the stained -ssues
more easily visible. It is alcohol and xylene insoluble and therefore compa-ble with most clearing agents and moun-ng media.
10. Moun/ng- Moun-ng seals the biological specimens and is an essen-al step before microscopic evalua-on. BioGenex SuperMount®
Permanent Moun-ng Medium (HK079-5K, HK079-7K) is a polymer based aqueous moun-ng media that does not require the use of a
coverslip. XMountTM Moun-ng Medium (HX035-YCD, HX035-10X) is a permanent moun-ng medium that has been op-mized for use
with BioGenex Xmatrx automa-on plasorm. BioGenex also offers Aqueous Moun-ng Medium (HK099-5K) - a glycerol-based moun-ng
medium that require the use of a coverslip. It is intended for short-term specimen storage and is compa-ble with most chromogens
and counterstains.
11. Washing steps – Typically 2-3 washes between each step are required. BioGenex Super Sensi-veTM Wash Buffer (HK583-5K) is a
phosphate buffered saline (PH 7.4) with surfactant and is the best choice in order to ensure op-mal staining. The buffer promotes
even spreading of an-bodies and other reagents to avoid inconsistent results.
A. An--MUC5AC
B. An--CD3 (T-Cell)
C. An--Glypican-3
5. For manual use (drop bobles) For i6000 Automa-on (Barcode labeled) For Xmatrx Automa-on (Elite - RFID tagged vials)
Detec-on Systems - Super Sensi-ve Polymer HRP (For mouse & rabbit an-bodies)
IHC Detec-on Systems - Links / Labels items-Manual
BioGenex Immunohistochemistry (IHC) Quick Guide
Super SensitiveTM Link-Label kits
SKU Size
Super
enhancer
Polymer-
HRP
DAB buffer
DAB
Chromo.
Peroxide
block
Power
block
Hematox.
EZ-AR Eleg.
1,2 & 3 Sol.
Nega/ve ctrl IgG
QD400-60KE
60
test
6 ml 6 ml 10 ml 2 ml 6 ml 6 ml 6 ml - 3 ml-Rabbit, 3 ml-Mouse
QD420-YIKE
500
test
50 ml 50 ml 50 ml 5 ml - - - - -
QD430-XAKE 1000 test 100 ml 100 ml 100 ml 10 ml - - - - -
QD440-XAKE 1000 test 100 ml 100 ml - - - - - - -
QD410-YAXE
i6000
200 test 20 ml 20 ml 5x10 ml 4ml 20 ml 20 ml 20 ml - -
QD550-YCXE
Xmatrx -Infinity
200 test 15 ml 15 ml 5x11 ml 4 ml 4x16 ml 21 ml 21 ml 16ml -
QD550-YCDE
Xmatrx-Elite
200 test 16 ml 16 ml
4x11 ml + 5 RFID
vials
7 ml 3x16 ml 3x16 ml 3x16 ml 3x16ml -
SKU Size Polymer-HRP DAB buffer DAB Chromo. Peroxide block Power block Hematox
EZ-AR Eleg.
1,2 & 3 Sol.
QD620-YIKE 500 test 50 ml 50 ml 5 ml - - - -
QD630-XAKE 1000 test 100 ml 100 ml 10 ml - - - -
QD610-YAXE
i6000
200 test 16 ml 4x11 ml 4 ml 3x16 ml 3x16 ml 3x16 ml -
QD610-YADE
Xmatrx-Elite
200 test 16 ml 4x11 ml + 5 RFID vials 7 ml 3x16 ml 3x16 ml 3x16 ml 3x16 ml
Detec-on Systems - Super Sensi-ve One-Step Polymer-HRP (For mouse & rabbit an-bodies)