http://www.genome-engineering.com/ Tips from genome engineering

1. TIPS

2. How to pick clones for a PCR
?

3. You need :
A plate
containing the PCR amplification mix
Primers, DNA pol, etc.
It exists on the market various ready-mix,
where only the primers (and bacteria) must be added.

4. You need :
A box of sterile tips
A "lid" box of tips

5. You need :
A petri dish
containing LB agar + antibiotic selection
Usually amp or kana
This petri dish must be gridded
and each square should be numbered.

6. You need :
A human can also
be helpfull

7. Pick a well isolated colony with a tip

8. No matter if you stick the point
into the agar

9. Scratch the tip with bacteria
on the Petri dish to a definite place ...
Little finger up !
It’s always hepfull

10. ... then put the tip to a definite place
of the PCR plate
For example,
the location #1 on the Petri dish
is the location A1 on the PCR plate,
#2 is A2,
#3 is A3,
etc.

11. Continue this process until you have
filled the entire plate of PCR or reach
the number of clones to analyze

12. Then, carefully remove
the lid.
The tips will be retained and will be
removed simultaneously from
the PCR plate.

13. Discard the lid and
put the PCR plate in
a thermocycler for
amplification

14. Music by Wasaru
Pictures from Cellectis bioresearch
Tip from Genome Engineering
The next one by you ?