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How foliage traits respond to the
vertical gradient and nutrient
amendment
Alexander Young
Climbing
vs
Shotgun
Sampling
Courtesy
of
Shinjini
Goswami
Relative growth rate was 13% higher in P plots in mid-age stands (p
= 0.02) and 15% higher in mature stands (p = 0.07). (Shinjini
Goswami 2017) unpublished.
Relative growth rate increase with P treated Trees
Question
- Which canopy traits would change if trees allocate
more biomass to aboveground biomass?
- Photosynthetic pigment content
- Leaf chemistry
- Leaf thickness (Specific Leaf Area)
Hypothesis:
- Specific leaf area will decrease with canopy positions at
different rates for different nutrient amendment treatments.
Specific Leaf Area: Surface area (cm^2) / mass (g)
Why would leaf thickness change?
•Palisade mesophyll
• Chloroplast center
Palisade mesophyll
Shade leaf
Sun leaf
https://en.wikipedia.org/wiki/Leaf
Methods
-SRT technique
-Only 4 trees (pilot)
-Every 2 meters sample
with pole pruner
Methods
-SRT technique
-Only 4 trees (pilot)
-Every 2 meters sample
with pole pruner
Leaves from 24 m
Leaves from 14 m
• Sun leaves have lower SLA
Smaller leaves, but weighed
proportionally more
• The phosphorus tree
maintained thin leaves
longer through the canopy.
Phosphorus: p = 0.03
CanopyPosition(m)
Future Directions
For the Summer
• Get replication! (Collect in 3 stands)
Future Lab Methods and Measurements
• Specific twig length
• Chlorophyll a/b spectroscopy
• Foliar Chemistry through vertical gradient
Acknowledgements
MELNHE 2017
• Ruth Yanai
• Dani Fernando
• Melany Fisk
• Trey Turnblacer
• Grace Haynes
• Claudia Victoroff
• Caitlin Buchheim
NSF-REU: Tardigrades in the Canopy
• Dr. Meg Lowman
• Dr. William Miller
USFS White Mtn. Natl. Forest
• Bartlett Exp. Forest
Discussion
Why would nutrient
treatment impact the
way trees deploy leaves
as a function of light
environment?

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How sun and shade leaves respond to N and P

  • 1. How foliage traits respond to the vertical gradient and nutrient amendment Alexander Young
  • 3. Courtesy of Shinjini Goswami Relative growth rate was 13% higher in P plots in mid-age stands (p = 0.02) and 15% higher in mature stands (p = 0.07). (Shinjini Goswami 2017) unpublished. Relative growth rate increase with P treated Trees
  • 4. Question - Which canopy traits would change if trees allocate more biomass to aboveground biomass? - Photosynthetic pigment content - Leaf chemistry - Leaf thickness (Specific Leaf Area) Hypothesis: - Specific leaf area will decrease with canopy positions at different rates for different nutrient amendment treatments.
  • 5. Specific Leaf Area: Surface area (cm^2) / mass (g)
  • 6. Why would leaf thickness change? •Palisade mesophyll • Chloroplast center Palisade mesophyll Shade leaf Sun leaf https://en.wikipedia.org/wiki/Leaf
  • 7. Methods -SRT technique -Only 4 trees (pilot) -Every 2 meters sample with pole pruner
  • 8. Methods -SRT technique -Only 4 trees (pilot) -Every 2 meters sample with pole pruner
  • 9. Leaves from 24 m Leaves from 14 m
  • 10. • Sun leaves have lower SLA Smaller leaves, but weighed proportionally more • The phosphorus tree maintained thin leaves longer through the canopy. Phosphorus: p = 0.03 CanopyPosition(m)
  • 11. Future Directions For the Summer • Get replication! (Collect in 3 stands) Future Lab Methods and Measurements • Specific twig length • Chlorophyll a/b spectroscopy • Foliar Chemistry through vertical gradient
  • 12. Acknowledgements MELNHE 2017 • Ruth Yanai • Dani Fernando • Melany Fisk • Trey Turnblacer • Grace Haynes • Claudia Victoroff • Caitlin Buchheim NSF-REU: Tardigrades in the Canopy • Dr. Meg Lowman • Dr. William Miller USFS White Mtn. Natl. Forest • Bartlett Exp. Forest
  • 13. Discussion Why would nutrient treatment impact the way trees deploy leaves as a function of light environment?