Find any images, links, video or other information about the photogr.docx

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Find any images, links, video or other information about the photographer Diane Rosenblum and her body of work entitled “ Clouds for Comment ?” post on open forum also share your feelings about how this body of work incorporate social media use your own words . ) Also pick one image to describe and discuss in detail. (hint hint there is text placed in her images-- from where and why) .

Education

Find a Web site that has an “About Us” section or a “Press Release” section. Write a three to four (3-4) page paper addressing the following: 1. Identify the Web site, the sender, and perceived receiver. 2. Analyze the integrated business communication. 3. Assess the media richness of the section. 4. Describe how the message is framed. 5. Identify opinion leaders. 6. Make recommendations for improving word choice. .

Find a video of a political ad for a state or national candidate i.docx

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Find a video of a political ad for a state or national candidate in the recent US election--choose an ad from a candidate you are sympathetic with. Then carefully analyze the logic of the ad [post a link to the ad]. Kenneth Burke says communication always reflects, selects, and deflects; that is, we choose words and images that present a version of events that lines up with our beliefs, goals or purposes. That is, the "whole truth" of a human situation is always more complicated than any particular description of it. How exactly does the ad "select"; that is, use words and images to persuade us? What ideas or perspectives does the ad "deflect"; that is, what's left out? How are ideas presented in incomplete or possibly misleading ways? What do you think of ads that do this? Obviously, it's fair to promote a point of view you believe in; how do we know when the line has been crossed and persuasion goes too far? Finally, what image of the United States do you think this reflects/presents to the rest of the world? .

Find a successful public service project that exemplifies effect.docx

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Find a successful public service project that exemplifies effective collaboration with different stakeholders, and summarize the results of the project in a PowerPoint presentation. (Please see the Suggested Reading in Unit V for examples.) 1) Ensure the PowerPoint presentation includes the location, key stakeholders involved (and their roles), time period to complete the project, and the challenges involved in completing the project. 2) Also, provide a brief critique of the project: 3) Is there anything that you would have done differently? 4) What elements of the project stood out to you? 5) Your presentation must be a minimum of 15 slides, not including title and reference slides. Suggested Reading Click the link below to view a video that highlights a New York City project that turned a rail line into a highly visible and popular walking trail—it is now used by hundreds daily. Amoeba films. (2012, March 19). NYC highline park: Ten minute tour [Video file]. Retrieved from https://www.youtube.com/watch?v=RKLucigE1yI .

Find a successful public service project that exemplifies effective .docx

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Find a situation (a real-world situation or a fictional situation) with two or more parties of jurisdiction or interest over a specific project or policy (public/private partnership, intergovernmental). At least one of the parties needs to be a governing board or elected officials. In 750-1,000 words, do the following: Explain the participants in the situation. (power involved, interest, requirement) Explain the difference between influence and negotiation among the parties. Recommend strategies to build the relationship between the parties in preparation for negotiation (at least one of the parties needs to be elected officials or a governing board) Recommend different techniques of negotiation the parties can utilize to get their interest or requirements met. Discuss how each party determines the value of what they are negotiating for and what they are willing to give up. Discuss what would make each party agree to a decision within a negotiation or leave the table (how much leverage does each party have). Use two to three scholarly resources to support your explanations. .

First CivilizationsAfter your reading and research, you shou.docx

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First Civilizations After your reading and research, you should have a basic picture in your mind of what it might have been like to live in those ancient cultures. To help clarify that picture, for this discussion, you are asked to role-play the part of a resident in ancient Mesopotamia, China or India. respond to at least two of your peers' character posts. Instructions Initial Post Imagine you resided in Ancient Mesopotamia, China or India and that we modern people had recently figured out a way to chat with you. You can play the part of any fictional citizen, ruler, priest or what-have-you. Whatever role you do assume, introduce yourself to your (present) peers in a post that covers the following points: A. Say who you are and what you do in your chosen civilization. Use actual terms from the period to make this come alive. B. Explain why your people created permanent settlements. C . Explain what you believe is the single (only one) greatest cultural achievement or innovation which you, as an ancient human, will leave for your descendants and why? Make sure to incorporate historical evidence and use appropriate citations as necessary. about 200 - 300 words .

First assignmentPrepare a perpetual inventory record using FIFO..docx

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First assignment Prepare a perpetual inventory record using FIFO. Prepare a perpetual inventory record using LIFO. Prepare a perpetual inventory record using average cost Second assignment Estimate the August 31 inventory using the gross profit method. Prepare the August income statement through gross profit for Whitewater Co. Calculate the Inventory Turnover Ratio for Whitewater for years 2009 and 2010. Provide a reason why the Inventory Turnover has changed from the previous year .

First Assignment World Cultures Assignment 1Paper, please see the .docx

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First Assignment: World Cultures Assignment 1/Paper, please see the assignment requirement, then check out the assignment example and then use the TEMPLATE given to complete the assignment (3 files). Original work only pleeeeeasee !! The Second Assignment: Criminal Law Assignment/Paper, please read the directions and do original work only (1 file), original work only !! Submit work by Saturday, July 27th 2019 by 9pm or sooner. .

First assignment 4-1Discussion Open Forum for Criminal Investig.docx

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First assignment 4-1 Discussion: Open Forum for Criminal Investigation During Weeks 4–7, you participate in an Open Forum Discussion concerning the criminal investigative analysis you are conducting for your assignments and Final Project. This is your opportunity to discuss aspects of the case and seek clarification about elements of evidence that will support your conclusion of the case and eventual report to the law enforcement agency that requested your services. When analyzing a case, it is essential that the analyst has access to all investigative material. As you review the case material, determine: Do you have all information relevant to the crime scene? Photos Reports Interviews Forensic Reports Do you have all information relevant to the medical examination? Autopsy Reports Autopsy Photos Forensic Reports Do you have all relevant information for the victim? In this Open Forum Discussion, you begin to collaborate with your colleagues to determine your initial thoughts about the case. Review the evidence of the case. What are your initial thoughts on the crime scene? What other things would you like to know about the victim? As you reviewed the documents, are there other areas about which you would like additional information? CASE STUDY I have addition file that need to be sent Second Assignment 2 Assignment: Investigating a Crime: Phase I – Organization and Summary of Information A local law enforcement agency has asked you to review the case evidence files of a crime and provide a report and presentation concerning the offender and the victim. All the evidence has been provided in the Learning Resources. It has been organized using a standard method for your convenience, but you might experiment with your own system of organization. The file titled “Document Index” will assist you locating specific evidence relevant to the case. For this Assignment, you will conduct Phase I of the process: Organization and Summary of Information. In a 2- to 3-page report: Provide the facts of the case concerning the offense, victim, and offender. Also, provide a summary of notes specific to the victim. This should include: Victim Current relationship status (e.g., single, married, divorced) Employment history Hobbies/routine activities Educational history Family history (e.g., parents, children) Personality issues Mental health issues Criminal history Substance/alcohol use abuse Use of social media .

First paragraph will Executive summary about our company 100 w.docx

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This document discusses a healthcare company called Health Network that provides three main products: HNetExchange for routing medical messages, HNetPay for managing payments and billing, and HNetConnect as an online doctor directory. It asks to identify risks, threats, weaknesses and the impacted domain for each of the seven IT domains: user, workstation, LAN, WAN-to-LAN, WAN, remote access, and system/application. It also provides instructions for weekly communication class assignments on introducing oneself and considering a future communication goal.

Firm of the Future Application Paper.Apply concepts of the f.docx

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Firm A and Firm B enter into a five-year currency swap. Firm A sen.docx

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Firm A and Firm B enter into a five-year currency swap. Firm A sends B $10,000,000, and in return receives ¥1,200,000,000 from Firm B. Firm A must pay 3% on the yen (to Firm B) while Firm B must pay Firm A 4% on the dollars. One year later the new swap rates are 2.5% USD and 1.5% JPY. The spot rate is ¥110/$. Ignore bid/ask spreads. What is the value of this swap to Firm A and to Firm B? 5 Experience in Solving a Problem Summary overview of my experience in creative problem solving Creative problem solving is an important tool in any organization for keeping good working relations in the workplace. It involves all the parties concerned with the problem hence encouraging different forms of creating thinking like group solutions, and brainstorming among others[footnoteRef:1]. In the past, I have been involved in numerous situations that required creative problem-solving. I realized that creative problem solving plays a crucial role in the process of making sure employees are able to address the issues they face as they discharge their work obligations. In such situations, I could engage all the employees and ensure they all participate in the process of coming up with the best solution. This could entail employees having representatives because all employees could not be engaged. The representatives would give their opinions concerning the best way to address the problem. I remember one day we had issues with increased costs of operating and the organization had to come up with strategies that could help in enhancing efficiency. All the staff members in my department were engaged to suggest their solutions to the problem. This is the time I realized the power of working in groups when addressing a problem affecting all the employees. In the decision-making process, giving the parties involved the chance to give their opinions concerning the problem makes others gain some more insights concerning the issue at hand. Engagement in problem-solving provides the opportunity for the members affected by the problem to share on the best strategies that can be embraced to address the issue. The different solutions can be analyzed and evaluated with the aim of settling on the best solution that is innovative and creative. [1: Proctor, Tony. Creative problem solving for managers: developing skills for decision making and innovation. Routledge, 2014.] I consider creative problem solving to be an important way of helping the decision makers to think outside the box. This entails thinking outside confines of what is acceptable to obtain the best solution for the problem. Being innovative in analyzing and evaluating possible solutions leads to the most creative solutions[footnoteRef:2]. For example, horizontal and vertical thinking are major modes of thinking that can help one to think outside the box. As a result, the best ideas can be provided to assist in the process of solving a problem in a creative manner. It ta.

First among Equals The Effect of Team Leader Characteristics.docx

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First among Equals: The Effect of Team Leader Characteristics on the Internal Dynamics of Cross-Functional Product Development Teams � Shikhar Sarin and Gina Colarelli O’Connor Drawing on the path-goal theory of leadership, the present study examines the effect of team leader characteristics on an array of conflict resolution behavior, collaboration, and communication patterns of cross-functional new product development (NPD) teams. A hierarchical linear model analysis based on a survey of 246 members from 64 NPD teams suggests that participative management style and initiation of goal structure by the team leader exert the strongest influence on internal team dynamics. Both these leadership characteristics had a positive effect on functional conflict resolution, collaboration, and communication quality within the NPD team while discouraging dysfunctional conflict resolution and formal communications. Comparatively, team leader’s consideration, initiation of process structure, and position had a surprisingly weak effect on internal team dynamics. Further, the findings underscore the differential effects on various dimensions of team dynamics, the importance of controlling for project and team characteristics, and the use of multilevel modeling for studying nested phenomena related to NPD teams. Impli- cations of these findings are discussed. Introduction R ecognizing the long-term competitive advan- tage offered by successful new product development (NPD), organizations are rely- ing heavily on cross-functional teams to improve their NPD processes (Barczak and Wilemon, 1992; Griffin, 1997; McDonough, 2000; Sarin and Mahajan, 2001; Sarin and McDermott, 2003; Ulrich and Eppinger, 1995; Wind and Mahajan, 1997). Typically these teams, composed of individuals drawn from a variety of functional specialties within the organization, are responsible for taking a product from conceptualiza- tion to commercialization. Growing popularity and anecdotal evidence notwith- standing, the results achieved from the use of cross- functional teams in NPD efforts have been decidedly mixed (Barczak and Wilemon, 1989; Katzenbach and Smith, 1993; Sarin and Mahajan, 2001). Among other reasons, this lack of consistent success has been at- tributed to poor project leadership, which often fails to appreciate the diversity of cross-functional teams and mismanages team dynamics—essential components to the performance of any NPD team (Henke, Krachenberg, and Lyons, 1993; Parker, 1994; Robbins and Finley, 1995). Effective project leadership has been identified as one of the most important mechanisms not only for managing team dynamics but also for steering the teams successfully and efficiently through the new product development process (McDonough and Griffin, � The authors are grateful to Tony Di Benedetto for processing this manuscript. They would also like to thank Robert Baron and Stacey Hills for their hel.

First Act of HamletAfter you have read Act I and the article, lis.docx

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First Act of Hamlet After you have read Act I and the article, list one example from Act I for each of the language patterns noted below. Also, provide answers for the questions included below. Syntax and Language unusual word arrangement omissions unusual words stichomythia: A line-for-line, verbal fencing match, used by the principal characters who retort sharply to each other, echoing their opponent's words and figures of speech. It is a play on words. What do you make of the fact that Hamlet's mother uses "thou", a familiar form of usage which she would use with her son and Hamlet's formal use of "you" when he would usually address her with the familiar form, "thou"? Does this tell the reader anything about the relationship between them at the moment? What other images and language devices has Shakespeare used here to show the conflict between Hamlet and Gertrude? (Act 1 Scene 2) Part B: Giving Advice In Act 1 Scene 3, much advice is given. Laertes provides brotherly advice to Ophelia regarding Hamlet’s affections, while Polonius gives fatherly advice to Laertes upon his return to school in France. Polonius then echoes Laertes’s advice to Ophelia regarding Hamlet. Analyze each piece of advice as follows: In one well-focused paragraph, explain the advice Laertes gives Ophelia regarding Hamlet’s affections. What does he counsel her against Hamlet? In one well-developed paragraph, contrast the advice given to Ophelia by Polonius and Laertes. Each counsels Ophelia against a relationship with Hamlet, but utilizes a different approach, citing different examples of why the relationship should not go forward. Explain. In one well-focused paragraph, compare the advice of your parent or guardian to that of Polonius’s advice to Laertes. .

First A Work in Progress submitting a 1,000 word position statem.docx

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First: A Work in Progress: submitting a 1,000 word position statement in which you declare an epistemological stance, and draw connections between your position and ideas presented in course readings and class discussions. these are the ideas from the whole class, + add some more about the important of validity and inquiry in research. Also, declare an epistemological stance as a pragmatism and subjective researcher, or reflect that I believe I will be a pragmatist and subjective researcher Reflection 1: Education research is defined as the scientific study field that evaluates education and the process of learning as well as the human attributes, interactions, institutions and organizations that determine educational results. Researchers in this field of study aims at describing, understanding and explaining the manner in which learning happens throughout the life of an individual as well as how the formal and informal circumstances affect every form of learning. As a doctoral student and a future researcher, this class helps me to understand and look beyond the information that I'm looking for. To understand the difference between a theory and facts .to be a researcher in the field of education you have to understand what educational prospect you have to follow, the present education, or what the future education will be. The idea of being a research for me is to add a new thing to the educational history, to find something that is missing or not covered the way it should be covered. As a special education doctoral student, and as a student in the epistemology class, I understand that my field is not that easy to research about because in many cases the environment and other factors may affect my results or it could change during the time. The human science and the educational field is related to the human part and what the situation around the study topic or what the society may reflect on about the topic. In Saudi Arabia, if we talked about special education, most of the time we couldn’t find a clear answer for our researchers because of the society or the families and what they needed or understood, which lead us to the most important thing. For me what I needed the most is for the society to understand why we are researching and why we are looking for an answer or new innovation in the educational field. The time is changing and the traditional way of learning is no longer effective, we need to create new ways to make the new generation learn and think. As a mission for the educational researcher, we should be open to other ideas, to look behind the facts, to read and learn more, this is the way to open up on the theory of learning and how to encourage ourselves to look more and discover new things for the future. Reflection2: The last couple of classes include to my knowledge a lot, and how to be an outstanding educator. From my point of view, the last classes were talking about the history of race and feminism. Thi.

Fingerprints may be one of the oldest forms of forensics, but how mu.docx

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Fingerprints may be one of the oldest forms of forensics, but how much does the average person really know. Consider how this week’s material may be relevant to your Week Six Final Paper. Prior to beginning this activity, please review the required resources: Read: An Introduction to Crime Scene Investigation Chapter 8: Fingerprint Evidence Fingerprint identification: How Far Have We Come? Review: How Crime Scene Analysts Collect and Preserve Fingerprints (Links to an external site.) Watch: Examination of evidence: Fingerprints and footprint (Links to an external site.) Where do fingerprints come from? (Links to an external site.) (Links to an external site.) Shining light on the evidence (Links to an external site.) Fingerprint analysis part 1: Types of fingerprints (Links to an external site.) Fingerprint analysis part 2: Fingerprint sub-classes (Links to an external site.) Fingerprint analysis part 3: Fingerprint minutia (Links to an external site.) Your report must focus on the discussion, collection, and analysis of fingerprints from a crime scene, and it must include the following: Explain the history of fingerprint analysis. Explain the characteristics of fingerprints. Describe how fingerprints are collected from a crime scene as well as how they are taken from a person. Explain how fingerprints are analyzed. Articulate what aspects of this week’s material you can use to complete your analysis of the crime scene in your Final Paper. The Fingerprint evidence paper, Must be approximately 1,000 words (approximately four double-spaced pages in length not including title and references pages), and formatted according to APA style as outlined in the Ashford Writing Center (Links to an external site.) . .

Fiore (2011) makes the statement, Its not what you said, but how y.docx

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Fiore (2011) makes the statement, "It's not what you said, but how you said it" (p. 64). Share an incident where you were on the receiving end of the non-verbal communication and how it impacted your reaction to the situation. Continue by sharing a time when your non-verbal communication sent the wrong message about something you were attempting to communicate. Focus on professional settings and the impact the non-verbal communication had on the outcome. REPLY TO MY CLASSMATE’S POST. EXPLAINING WHY YOU AGREE WITH HIS POST TO THE ABOVE DISCUSSION (A MINIMUM OF 150 WORDS EACH) CLASSMATE’S POST In the field of sociology, I as a student studied the human, but in my second field work in the field of anthropology I as a student was better able to study the human in society. In the field of education, I work as a Freedom Writer for Teacher Training Institute's and for the foundation we work through convention in the field of education for teacher teaching curriculum K-12. Each year we host teacher training programs for teachers locally, internationally, and nationally across America focusing on cross-cultural and universal experiences in the field of education. We focus on activities that lend itself to verbal and nonverbal communication. As Freedom Writers we role play one activity is to invite teachers to participant in an activity that focuses on Dad and Mom meeting their daughters first boyfriend. He knocks on the door they invite him in. After the initial meeting there is nervousness, insecurities, and fidgeting from boyfriend as Dad clears his throat the boyfriend consistently avoids eye contact during this activity. Mom on the receiving end of the non-verbal communication let's Dad lead out of fear of rebellion and their daughter never returning home after their first date. They sit down to eat dinner before allowing their daughter to leave and the boyfriend begins to perspire. Perspiring is a non-verbal sign that is non-threatening as he is trying to impress his girlfriend’s mother and Father. He brought her flowers and was polite at the end of diner they said yes and allowed him to take out their daughter. As they left with verbal communication thanking the mom and Dad in this activity the two began to smile expressing non-verbal communication after their approval. This is only one of many activities created for a safe environment and a safe learning outcome by The Freedom Writers and teacher training program in the field of education. This activity really works, is effective and does resonate with teacher student communications.0 .

Find an example of a real-world project using the Internet. Iden.docx

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Firebase is a Real Time database system, that stores, synchroniz.docx

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Firebase is a Real Time database system, that stores, synchronizes and shares user data in milliseconds. It is easy to setup and configure with your application. It also provides many tools and services to the developers such as, “authentication services” which manages user access in a secure manner with many authentications such as, username & password and Facebook and Google accounts. “Cloud Storage” allows users to store & share their media information like images, videos, audio clips, and Real Time database service that synchronizes user data across devices in real time–thereby allowing developers to accomplish their tasks in an effective manner. MySQL is one of the relational database management systems provided by Oracle. It provides high levels of security, since it is managed by the database administrator, who can control & manage the entire database. It also provides a highly secure environment, with scalability that facilitates controlling large amounts of stored data while allowing the database expansion process in the future. While MySQL has many strengths,, it also has some limitations that affects development. Stability is one of the key weaknesses that makes MySQL less reliable than others, since it has limited functions & transactions that make the development process to be very limited. Sometimes MySQL may have poor performance scaling, which it makes the process of dealing with many simultaneous operations much more difficult. Another Backend database system is MongoDB. This is a NoSQL and non-relation database. It is a document database that stores data in a flexible JASON as documents, which allows users to store data of different types. Although MongoDB provides flexibility and many other services to the developer, it has many strengths and weakness. One of its strengths is its high speed, since MongoDB provide access to the document by indexing which makes the retrieving process much faster. MongoDB also provides quick accessibility to data, since it has features like replication and gridFS, which enhance the speed of the data availability. MongoDB also has some weaknesses that affect reliability. One of the weaknesses is that MongoDB does not support the joint, like other relational database systems, which makes adding and updating processes less efficient. But there is solution for it that affects the performance and the execution aspect. In the implementation section, I have explained and illustrated the kind of Backend system that has been used for the project along with my rationale. When the DBMS selection process was completed, developing and creating an iOS Mobile application process was started. The iOS Mobile Application is the base platform for the targeted system. It includes many features that gives the application several privileges & proprieties, so the selection process of the most effective features process has been applied (see Appendix table.1). When the development and creating process was compl.

Finish the term paper using the following outline. In addition to th.docx

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Finish the term paper using the following outline. In addition to the 4-6 pages of the paper itself, you must include a title page and a reference page. You are to follow APA Guidelines for citing and referencing sources. Your paper must be in your own words, representing original work. Paraphrases of others' work must include attributions to the authors. Limit quotations to an average of no more than 3-5 lines, and use quotations sparingly. It is always better to write the information in your own words than to directly quote. Title: Penalties and adjudication for various information and technology offenses. Thesis: Penalties and adjudication for various information and technology offenses are not playing the crucial role of securing the abolition of breaching the law, which has been set, to safely guide the key players in the platform. The law is not even ensuring that those people who temple with the originality of a given person work. i. Introduction a. Thesis b. The role of the thesis. ii. Expounding on the law and some of the changes needs to make it better. a. Background of the law b. Some of the recommendation that has been pointed out concerning the law by different stakeholders. c. The effects of the law on today's information and technology industry. iii. The main objectives of the law. a. The success and the future of IT in the country b. The goals of addressing the topic c. The significance of the law and the setbacks d. The significance of implement the law to the grass root. iv. The benefit of law to the industry a. The key traits of for success b. The goal of the law c. Efforts to introduce new laws and ensuring they have been followed. i. Significance of changing some articles in the law ii. Incorporating of organs to ensure that the effectiveness of the law d. Making sure that the law meets the national and international standards v. Recommendation from the writer's view. a. Some of the things that can make a difference in the information and technology sector. vi. Conclusion a. Summarizing the arguments and efforts made Reference Bottoms, A. (2001). Compliance and community penalties. Community penalties: Change and challenges, 87-116. Roth, M. P. (2010). Crime and punishment: A history of the criminal justice system. Nelson Education. .

Find the company’s selected for the Week 2 assignments (Domin.docx

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Find the company’s selected for the Week 2 assignment's (Domino’s Pizza) annual report from SEC.gov or the investor relations section of the company's website. Be careful not to use quarterly reports. Prepare a PowerPoint presentation in which you describe the relationship between strategic and financial planning. Include the following. Use the Notes feature in PowerPoint to provide your analysis. A strategic planning initiative for the organization identified in the Week 2 assignment - Identify an initiative discussed in the organization's annual report. that occurred in the preceding three or four years How the initiative affects the organization's financial planning How the initiative carried through to the present and impacted the entity's financial performance How the initiative affects costs and revenues of the supply chain Ethical concerns related to the initiative Format your presentation consistent with APA guidelines. .

Find an article in the University Library or on the Internet tha.docx

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Find an article in the University Library or on the Internet that discusses a negotiation situation that has occurred in a global context (e.g., international organization or corporation, international acquisition, government-to-government negotiation, etc.). Analyze the implications of globalization and technology on the negotiation in a 1,050- to 1,400-word paper. Provide a copy of the article you used. Book used in class: Lewicki, R. (2014). Negotiation (7th ed.). McGraw-Hill Higher Education. .

Finding Km Values httpwww.brenda-enzymes.org F.docx

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Finding Km Values http://www.brenda-enzymes.org/ Finding Km Values • BRENDA – Kinetic activity database – Catalogs enzyme activity and other kinetics-focused papers – EC Number • #.#.#.#(##) – Identifies enzyme – Not species specific – Can also search for substrates & ligands Ex: Find Km for Succinyl-CoA Synthetase Tissue-Specific in Humans GDP-Forming: Anabolic Metabolis ADP-Forming: Catabolic Metabolis Finding Km ValuesFinding Km ValuesEx: Find Km for Succinyl-CoA SynthetaseSlide Number 4Slide Number 5 Review1-10000000001-10000000001-1-1000000001-100000000101-1-10000000001-10000000101-1 Draw the pathway 1-10000000001-10000000001-1-1000000001-100000000101-1-10000000001-10000000101-1 100000000-1010000000-1001000000-1000101000-100001-10000000000101-100000001-10 rref Rank: Nullity: Dimension: Free variables: Find J E+S ES E+P k1 k-1 k2 k-2 Previously we looked at rapid equilibrium (kp ~ k2) and therefor the [P] depended only on k2[ES] rate. Michaelis-Menten is useful in calculating enzyme kinetics of a system where a substrate can reversibly bind to an enzyme Under quasi-steady state assumption, we assume that the change of concentration of the enzyme and enzyme-substrate complex is equal to zero The maximum velocity is the rate of the reaction at which the enzyme is saturate with substrate Total enzyme is distributed between E and ES (ET = E + ES) How to derive Rate Equations Draw reaction scheme of all steps Use mass action kinetics to write ODEs for concentration changes such that the right hand side contains all producing and consuming reactions Determine total enzyme Use quasi-steady state assumptions and E(total) to derive algebraic equations for concentration of enzyme The reaction rate v is equal to the rate of product formation E+S ES E+P k1 k-1 k2 k-2 There enough substrate that ES concentration never really changes (E and ES reach equilibrium) Enzyme is neither produced nor consumed 5 From Lecture 11: Kinetics of enzymatic reactions Where is this from? What assumptions are made if it is quasi-steady state? Must show how this was attained in project Example of disease: Tuberculosis Caused by mycobacterum tuberculosis (MTB) MTB is an aerobic, nonmotile bacilus Can remain latent in its host One of the top ten causes of death around the world Multiple instances of total drug-resistant TB Virulence Pathway Phagocytosis by a macrophage is a multi-step procedure that ensures complete degradation Once a pathogen is engulfed, it enters a phagosome which then fuses with a lysosome (phagolysosome complex) The lysosome has all the needed components to digest the pathogen MTB is able to remain and reproduce in the phagosome and inhibit the formation of the phagolysosome As a secondary response, the lungs create granulomas to contain the pathogen Pathway of Interest The glyoxylate cycle (glyoxylate shunt) is an alternative anabolic pathway to the tricarboxylic acid c.

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