5. Ovaries
FUNCTIONS:
• The production of gametes (gametogenesis –
oogenesis)
• =Endocrine: secretion of steroid hormones
(estrogens & progesterone). Estrogens promote
growth and maturation of internal and external sex
organs & are responsible for the typical female
characteristics that develop at the time of puberty.
They also act on mammary glands to promote
breasts development.
• Progesterone prepare sex organs, mainly the uterus,
for pregnancy by promoting secretory changes in the
endometrium . It prepare the mammary glands for
lactation.
6. OVARY
•
•
•
The surface of the ovary is covered with surface epithelium (is
known as the germinal epithelium ), a simple epithelium which
changes from squamous to cuboidal with age. Immediately
beneath this surface epithelium there is a dense connective tissue
sheath-tunica albuginea.
The ovaries are composed of an outer cortex-and inner medulla-
The cortex is composed of ovarian follicles-(developing oocytes-
with their associated follicular cells), interstitial gland cells and
stromal elements .ovarian follicles are in different stages of
development (least mature to most mature):
– Primordial
– Primary
– Secondary
– Mature (vesicular, Graafian follicle)
Medulla contains loose connective tissue, blood vessels, lymphatic
vessels & nerves.
7.
8.
9. Ovarian follicles-םיקיקז-
• 400.000 – 800.000 oocytes present at the
birth.
• During the reproductive life span, a women
produces only about 400-500 mature ovum.
• Most of them degenerate- ןווינby atresia-
98%) רבעמ
יא ).
• Provide the microenvironment for the
developing oocytes.
• Normally, only one follicle completes
maturation in each cycle.
10. Primordial follicles
• At first appear in the ovaries during the third month
of fetal development – תירבוע
תוחתפתה
• Are most numerous
• Are located at the periphery of the cortex
• Each consists of a primary oocyte and a single layer
of flattened follicular cells, the outer surface of which
is bounded by a basal lamina. The nucleus of the
oocyte is positioned eccentric-הנושמ- in the cell. It
appears very light and contains a prominent –טלוב-
nucleolus. Most organelles of the oocyte aggregate-
םידחאתמ in the centre of the cell, where they form the
vitelline body.- )ח>הציב )ןילטיו
13. Primary follicles
• Is the first stage of the growing follicle
• It consists of a primary oocyte with a single layer of
cuboidal /columnar follicular cells. As development
proceeds, the number of follicular cells increases by
mitosis forming several layers around the primary
oocyte (membrana granulosa).
• Between the primary oocyte & the adjacent-
ךומסfollicular cells appear zona pellucida (gel-
like substance that is rich in
glycosaminoglycans- ןובלח=ריר+ רכוסand
proteoglycans).
• Stromal cells, surrounding the follicle, form a sheath of
connective tissue cells, known as the theca folliculi.
• During each cycle, a few primary follicles will continue
to develop into secondary follicles.
17. Secondary follicle
•
•
•
•
It consists of primary oocyte, that is surrounded by zona pellucida
Membrana granulosa begins to secrete follicular fluid. Small
pockets of fluid between granulosa cells begin to appear.
Previously undifferentiated stromal cells now develop into two
distinct layers around the developing follicle: the theca interna
and the theca externa . Cells in the theca interna are large,
rounded and epithelial-like, in response to LH-stimulation they
synthesize and secrete the androgens that are precursors-- םידקמof
estrogens. The theca external is the layer of connective tissue
cells. It mainly contains smooth muscle cells and bundles of
collagen fibers.
The secondary-vesicular follicle is characterized by the presence
of pockets of follicular fluid within the membrana granulosa. As
the follicle continues to develop, the separate pockets fuse to form
one large pocket of fluid called the follicular antrum.
20. Graafian follicle
• As the secondary follicle increases in size, the antrum
also enlarges.
• The primary oocyte migrates at the periphery
• The granulosa cells form a thickened mound- –םימרענ
the cumulus oophorus
• The cells of the cumulus oophorus surround the oocyte &
remain with it at ovulation- –ץויב corona radiata
• At time of ovulation takes place the first meiotic
division, that gives rise to the secondary oocyte & the
first polar body.
• If in the oviduct takes place the fertilization- הירפה-is
happened the second meiotic division & the secondary
oocyte transforms into the mature ovum.
• If there is no fertilization the secondary oocyte will die in
48 hours.
21. GRAAFIAN FOLLICL.E
theca e-xterna.-- - - - .
theca interna
'"antrum
"
corona
radJata.
..,.,.
■ +:-.. -
r . " - "
· , .ood vessels
:;.-)i.r-..;..,...._.• ._ - .. •·· ••
a09'lls
liha!
. me
a rad.la
·QV!lalkin
a
:'.v
. .
- antru.m."
Liquor
folliculi
_. • • ■
•
' I , I ' I
• J
M
A
T
I
U
IFUiG!AAAFl1
IUi FOII.LIOLE
22.
23.
24.
25. FOLLICULAR
CELLS 0R
GRANJ.':LdS;,
1:IQUOR
FOLLICULI
TH.ECA.
INTERNA
'tHECA
EXTERNA
None No1}6 'None
Single layer of flat
cells
N6,w-· No11e Norte
Single-layer of cu-
boidal cells
•,
PrioJRry oocyte Present and mi
crovilli of pri
mary oocyte
fotm gap junc
tions witJ1 610-
podia of co
rona radiata
cells
Several layers of
follicular ceJls
(now called
gnmulosa cells}
Spaces develop
betwe$:)n grni1u
lo a cells
None PreserH Pre;ent
Accumulate
in'.spai,es
,between
grantiJos
cell ·
.Pre
-.sent:.
- . . ,'
.·'
26. Ovulation -ץויב
• is a hormone-mediated process
resulting in the release of the
secondary oocyte
• takes place at the middle of
the menstrual cycle
• is stimulated by LH
• the factors include:
- increase in the volume
and pressure of the
follicular fluid
- contraction of smooth
muscle fibers in the theca
externa
27. Posterior wall
of uterus
Blastocysts
Morula Eight-cell
stage
Zygote Oocyte
penetrated
by sperm
Growing
follicle
Early primary
follicle
Corpus albicans
Mature corpus luteum
Atretic (degenerating) follicle
Endometrium
Mature
follicle
Blood
Released oocyte
Ruptured follicle
Connective tissue] Developing
Coagulated blood corpus
luteum
28. • In primordial, primary and
secondary follicles, primary oocytes
are arrested in prophase of first
meiotic division.
• The LH surge causes the oocyte to
complete the first meiotic division and
begin the second, which is arrested in
metaphase. It is now called a
secondary oocyte.
• If the secondary oocyte is fertilized,
the 2nd meiotic division will be
completed.
Meiosis of Oocytes
1st polar
body
may divide
to
produce
2 non-
functional
polar bodies
2nd polar
body
29. Corpus luteum (yellow body)
•
•
The collapsed follicle undergoes reorganization into the corpus
luteum after ovulation.
In the corpus luteum development are distinguished next phases:
proliferation-, morphological changes, flowering & involution.
•
•
Two types of luteal cells are identified:
- granulosa lutein cells, very large, centrally located cells derived
from the granulosa cells. The granulosa cells undergo hyperplasia
(proliferation), hypertrophy (enlargement) and are transformed
into granulosa lutein cells.
- theca lutein cells, smaller, peripherally located cells derived
from the cells of the theca interna layer
The resulting structure is highly vascular. If fertilization occurs,
the corpus luteum persists - דימתמand secretes progesterone and
estrogens. These hormones stimulate the growth and secretory
activity of the endometrium, to prepare it for the implantation of
the blastocyst. ערזה
לש
הטבנה
30. OVULATION AND CORPUS LUTEUM
OVULATION
CORPUSALBICANS
(connective tissue scar)
blood
vessels
31.
32. Corpus luteum (yellow body)
•
•
•
•
Are distinguished corpus luteum of pregnancy & corpus
luteum of menstruation-תסו- (is formed in the absence of
fertilization)
Corpus luteum of pregnancy is formed after fertilization and
implantation. Human chorionic gonadotropin- םיכשאו
תולחש
(hCG), secreted by the trophoblast- תירפומה
תיציבב
תינוציח
הבכש of
the chorion - תיסיסstimulates the corpus luteum & prevents its
degeneration.
hCG can be detected in the serum as early as 6 days after
conception & in the urine as early as 10-14 days of pregnancy.
Detection of hCG in the urine forms the basis of most
pregnancy tests.
33.
34. Fate of Corpus Luteum (CL)
without fertilization
• FSH and LH release is inhibited by the
progesterone and estradiol released by the CL .
• If fertilization and implantation do not occur,
the corpus luteum remains active only for 14
days. It degenerates and is replaced by
connective tissue forming a corpus albicans ,
that slowly decrease in size but never
disappears.
36. GnRH
LH maintains the
corpus luteum
Progesterone &
estrogen from CL
inhibits GnRH ,
slowing the release
of FSH and LH
Without LH, the
CL degenerates
Junqueira et al. 8th ed. Appleton and Lange
X X
39. Fate of Corpus Luteum with fertilization &
•
•
•
•
implantation
Corpus luteum of pregnancy is formed after fertilization and
implantation.
Human chorionic gonadotropin (hCG), secreted by the trophoblast of
the chorion stimulates the corpus luteum & prevents its degeneration
for ~ 6 months (hCG can be detected in the serum as early as 6 days after conception
& in the urine as early as 10-14 days of pregnancy. Detection of hCG in the urine forms
the basis of most pregnancy tests).
also estrogen, IGF-I and II (from ovary), LH, prolactin (from ant.
pituitary) and insulin contribute to formation and maintenance
CL.
– CL grows to a diameter of 5 cm
– Continues to secrete progesterone
– Decreases in size during last 3 months
Begins to secrete relaxin (causes dilation of cervix, softens
symphysis pubis ) in preparation for birth.
40. CORPUS LUTEUM OF PREGNANCY
•''
•
·..
•
t. '
<c:ORPUS.
ALBIC;NS· .,
..
t
·.
• ' .
•i'· •.
.''
.' '···
.
,.
'
• , ,;;_•.;,_,--,..
41. Ovarian cycle has 2 phases
• FOLLICULAR PHASE – consists of
the development of a primordial follicle
into a mature or Graafian follicle
(dominant follicle).
• LUTEAL PHASE – consists of the
formation of the corpus luteum, a
major-secreting gland.
• At the middle of the ovarian cycle the
OVULATION takes place.
43. Fate of Non-ovulated Follicles
• Although several secondary follicles are
growing during each cycle, only one is
ovulated
• Remainder of follicles in the cohort undergo
atresia
• Atresia occurs via genetically programmed
cell death (apoptosis) and can occur at any
stage of follicular development
44. Follicular atresia or Degeneration
• Such degenerate follicles are called atretic
follicles. Sometimes they appear to contain a
dark pink-staining material which is probably the
remains of the zona pellucida of the follicle.
• Large numbers of follicle undergo atresia during
fetal development, early postnatal life & puberty.
• After puberty, during a reproductive cycle a group
of follicles starts the maturation process;
normally, only one or two follicle complete its
maturation and are eventually ovulated.
45. 1. Oocyte showing signs of degeneration (multiple stages of this).
2. Stratum granulosa showing signs of disorganization.
3. Follicular cells separate from basal lamina and oocyte.
4. Glassy membrane (between granulosa and theca interna) is a
sign of advanced atresia.
46.
47. OVIDUCT
The uterine tubes (also called Fallopian tubes or oviducts):
Functions:
•
•
•
•
transport the ovum from the ovary to the site of fertilization
help transport spermatozoa from the site of deposition to the
site of fertilization
provide an appropriate environment for fertilization
transport the fertilized ovum (embryo) to the uterine horns
where implantation and further development may occur.
The uterine tubes can be divided into four major parts:
•
•
•
•
the infundibulum
the ampulla
the isthmus
the uterine or intramural segment
48. (intramural)
~ 1 cm long
(2/3 of
total length)
Bloom and Fawcett, 12th ed. Chapman and Hall
open to peritoneal cavity
Fimbriae
(fringed extensions)
49.
50. Oviduct
The oviduct is a typical
tubular organ composed
of:
• Tunica mucosa
• Tunica muscularis:
smooth muscle tissue, 2
layers: inner – thicker,
circular & outer –
thinner, longitudinal
• Tunica serosa:
connective tissue basis
that is covered by
mesothelium
51. Oviduct
• Tunica mucosa with a simple columnar
ciliated epithelium and a lamina propria.
There is no lamina muscularis mucosae in
the oviduct.
The tunica mucosa is highly branched and
folded, especially in the infundibulum and
ampulla.
52. Epithelium of the oviduct
Contains two types of cells:
• Ciliated cells; ciliary beating causes caudal fluid
flow, to move the oocyte toward the uterus; Estrogens
increase the rate of the cilliary beat. During luteolysis,
ciliated cells lose their cilia (deciliation). Estrogen
increases the proportion of ciliated cells.
• Non-ciliated secretory cells called "Peg cells“, are
less numerous than the ciliated cells. They produce
the oviductal fluid that provides nutrients to the egg
during its migration. Progesteron increases the
proportion of secretory cells.
57. Uterus
Functions:
1. serves to receive the sperm
2. transports sperm from site of deposition to uterine tubes for
fertilization
3. provides suitable environment for
a. implantation of the embryo
b. nourishment of the embryo & fetus during pregnancy
4. provides mechanical protection of the fetus
5. expels the mature fetus at the end of pregnancy
In the fundus and body of the uterus, the wall is divided into the:
•
•
•
Endometrium = tunica mucosa (basal & functional layers)
Myometrium = tunica muscularis
Perimetrium = tunica serosa
61. Perimetrium
• is the tunica serosa of the uterus. It has the
typical composition of loose connective
tissue with mesothelium, but contains a
large number of lymphatic vessels.
62. Myometrium
• Submucosal layer – smooth muscle
bundles are oriented parallel to the long axis
of the uterus
• Vascular layer – is the thickest layer.
Contains numerous large blood and
lymphatic vessels. Smooth muscle bundles
are oriented in a circular or spiral pattern
interlaced with each other.
• Supravascular layer – the arrangement of
smooth muscle bundles is the same as in the
submucosal layer
64. Endometrium
•
•
•
•
The epithelium is usually simple columnar
ciliated.
The lamina propria consists of loose
connective tissue full of neutrophils and
lymphocytes.
Uterine glands are simple tubular
nonbranched glands located in the lamina
propria.
Contains a system of blood vessels
65. The endometrium proliferates and then degenerates
during a menstrual cycle
• Throughout the reproductive life span, the endometrium
undergoes cyclic changes each month that prepare it for
the implantation of the embryo. Changes in the
secretory activity of the endometrium during the cycle
are correlated with the maturation of the ovarian
follicles.
• During reproductive life, the endometrium consists of 2
layers that differ in structure & function: functional
layer & basal layer.
• Functional layer – the thick part of the endometrium,
which is sloughed off at menstruation.
• Basal layer – serves as the source for regeneration of
the functional layer.
69. Spiral arteries: dependent
upon estradiol for growth,
progesterone for
maintenance
Capillary bed with dilated
portions of capillary wall
(lacunae)
70. Menstrual cycle
The endometrium is directly controlled by
OVARIAN hormones (estrogen,
progesterone), not by pituitary hormones
Menstrual cycle has 3 phases:
• Proliferative phase is regulated by estrogens.
• Secretory phase is under the control of
progesterone.
• Menstrual phase results from a decline in the
ovarian secretion of progesterone & estrogens.
73. Proliferative phase
• under control of estrogenes
(follicular phase of ovarian
cycle) the stromal and
epithelial cells in the
stratum basalis begin to
proliferate
• epithelial cells in the basal
portion of glands rapidly
proliferate, reconstituting
the glands and migrating to
cover the denuded
endometrial surface
• stroma, glands, spiral
arteries grow toward lumen
77. Secretory phase
- under control of
progesterone (luteal
phase of ovarian cycle
- uterine glands coiled,
larger lumens
- secrete glycogen,
mucin
- arteries become more
coiled, extend nearly to the
surface of the endometrium
- stromal cells transform in
desidual cells (rich in
glycogen)
80. Menstrual phase
- the involution of the corpus
luteum results from a decrease
in blood levels of steroid
hormones, leading to an
ischemic phase.
- a reduction in the normal blood
supply-causing intermittent
ischemia - and the consequent
hypoxia determine the necrosis
of the functional layer of the
endometrium, which sloughs off
during the menstrual phase.
81. Menstruation
At end of luteal phase of ovarian cycle,
Progesterone Spiral arteries to constrict
84. Ectocervix
Cervical Os
Endocervix (glands)
-not sloughed off
during menstrual
phase
Fornix of vagina
next slide
The internal surface of the cervix (endocervix or cervical
canal) is covered by a simple columnar epithelium that secretes
mucous and invaginates into the cervical wall to form branched
tubular mucous-secreting glands which lubricate the vagina.
85. Endocervix with
branched tubular mucous
glands
Mucus:
• Thick during follicular and
luteal phases to impede
sperm and bacterial entry
• Increase in volume,
decreased viscosity
enhances sperm entry
during ovulation (due to high
levels of estradiol)
Cervical
Os
86. Cervical
Os
Wall of
Vagina
Pap smears
Dense CT
and
Smooth muscle
adapted from Bloom and Fawcett, 12th ed.
Chapman and Hall
The epithelium of ectocervix is stratified squamous epithelium continuous
with the vaginal epithelium. This transition zone is utilized for Pap smears, as it is
the primary site of cervical cancer.
88. Vagina
• The vagina serves and the receptacle for the penis during copulation and
also expels the fetus at birth, serving as the birth canal.
The vagina is a fibromuscular tube with a wall consisting of three layers:
the mucosa, muscularis and adventitia of the vagina.
•
•
• Mucosa: The stratified squamous epithelium (deep stratum basalis,
intermediate stratum spinosum, superficial layers of flat eosinophilic
cells which do contain keratin but which do not normally form a true
horny layer) rests on a very cellular lamina propria (many leukocytes).
Towards the muscularis some vascular cavernous spaces may be seen
(typical erectile tissue).
Muscularis: Inner circular and outer longitudinal layers of smooth
muscle are present. Inferiorly, the striated, voluntary bulbospongiosus
muscle forms a sphincter around the vagina.
Adventitia: The part of the adventitia bordering the muscularis is fairly
dense and contains many elastic fibers. Loose connective tissue with a
prominent venous plexus forms the outer part of the adventitia.
89. Vagina
•
•
•
•
•
The lamina propria contains many elastic fibers, a large
venous plexus and NO GLANDS.
Lubrication is provided by the cervical glands and by the
vestibular mucous glands (present at the opening [vestibule]
of the vagina; a.k.a. Bartholin’s glands).
The epithelial cells are continually desquamated and contain
much glycogen when estrogen levels are high (ovulation).
Bacteria in the vagina ferment the glycogen to form lactic
acid, thus lowering the pH. The acidic environment inhibits
the growth of some pathogenic microorganisms.
Post-menopausal women do not secrete much glycogen
because of their low estradiol levels; the subsequent higher
vaginal pH can lead to increased vaginal infections.
90.
91. Glycogen Lactic acid
Vagina
LAMINA PROPRIA:
Numerous
elastic fibers
Veins
No glands!
Lubricated by cervical
mucus and vestibular glands
Bacterial
fermentation
Non-keratinized
Non-keratinized
Ross et al. 2003 LWW
Lamina propria
