Escherichia coli
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There is no doubt that the Magic™ Escherichia coli Expression Service is capable of providing the magical biopharmaceutical products with superior efficiency and productivity in the world, especially for the large-scale production. https://www.creativebiolabs.net/magic-escherichia-coli-expression-service.htm
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6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
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Thematic analysis in qualitative research is a time-consuming and systematic task, typically done using teams. Team members must ground their activities on common understandings of the major concepts underlying the thematic analysis, and define criteria for its development. However, conceptual misunderstandings, equivocations, and lack of adherence to criteria are challenges to the quality and speed of this process. Given the distributed and uncertain nature of this process, we wondered if the tasks in thematic analysis could be supported by readily available artificial intelligence chatbots. Our early efforts point to potential benefits: not just saving time in the coding process but better adherence to criteria and grounding, by increasing triangulation between humans and artificial intelligence. This tutorial will provide a description and demonstration of the process we followed, as two academic researchers, to develop a custom ChatGPT to assist with qualitative coding in the thematic data analysis process of immersive learning accounts in a survey of the academic literature: QUAL-E Immersive Learning Thematic Analysis Helper. In the hands-on time, participants will try out QUAL-E and develop their ideas for their own qualitative coding ChatGPT. Participants that have the paid ChatGPT Plus subscription can create a draft of their assistants. The organizers will provide course materials and slide deck that participants will be able to utilize to continue development of their custom GPT. The paid subscription to ChatGPT Plus is not required to participate in this workshop, just for trying out personal GPTs during it.
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Cytokines and their role in immune regulation.pptx
This presentation covers the content and information on "Cytokines " and their role in immune regulation .
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Escherichia coli
- 1. An effort to engineer E. coli for the production of difficult-to-express antibodies has been made. For example: 1) E. coli strains with mutations in the glutathione and thioredoxin reductase in combination with coexpression of cytoplasmic chaperones GroEL/ES, trigger factor, DnaK/J as well as signal sequence-less variants of periplasmic chaperones DsbC and Skp increased the yield of functional Fab (Figure. 2). 2) Coexpression of Erv1p sulfhydryl oxidase increased the yield of camelid single domain antibodies (VHH) in the cytoplasm. Despite these efforts, most antibody fragments are produced in the periplasm of E. coli using N-terminal leader sequences targeting the periplasmic Sec-secretion pathway. Figure. 1 Conventional antibody expression in E. coli. Most of the recombinant antibodies rely on the formation of disulfide bonds in order to reach their native structure. Periplasm is the only E. coli oxidizing compartment compatible with disulfide bond formation and consequently it has been considered the logic environment for antibody accumulation despite its small volume. Mutant strains (gor-/trxB-) with partially oxidizing cytoplasm represent an alternative (cytoplasmic accumulation) as well as expression systems that enable the antibody secretion in the medium or at the cell surface (antibody display). (Ario de Marco. Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application‑specific needs)